Abstract:
:The binding of iron-loaded human transferrin at the surface of Neisseria meningitidis is mediated by two polypeptides, Tbp1 and Tbp2. Predicted Tbp amino acid sequences from N. meningitidis strains are highly divergent. This variability is particularly pronounced throughout the Tbp2 polypeptide. In this study, a highly structured and extremely stable Tbp2 domain of about 270 to 290 amino acids which is involved in the binding to transferrin and whose position is well conserved has been characterized. The conservation of such a remarkable structure in a very divergent protein domain (there is only 43% amino acid identity within this region) suggests that is plays an essential biological role and raises a number of questions regarding tbp2 evolution.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Vonder Haar RA,Legrain M,Kolbe HV,Jacobs Edoi
10.1128/jb.176.20.6207-6213.1994subject
Has Abstractpub_date
1994-10-01 00:00:00pages
6207-13issue
20eissn
0021-9193issn
1098-5530journal_volume
176pub_type
杂志文章abstract::Burkholderia thailandensis is a soil saprophyte that is closely related to the pathogen Burkholderia pseudomallei, the etiological agent of melioidosis in humans. The environmental niches and infection sites occupied by these bacteria are thought to contain only limited concentrations of oxygen, where they can generat...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00304-20
更新日期:2020-11-04 00:00:00
abstract::After frog auricles have been in contact with a suspension of bacteria or bacteria-free supernatant fluid, newly synthesized bacterial ribonucleic acid (RNA) is recovered in animal cells. It appears that the presence of bacterial deoxyribonucleic acid (DNA)-dependent RNA polymerase is necessary for the transcription o...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.114.1.114-120.1973
更新日期:1973-04-01 00:00:00
abstract::Cadmium-resistant Pseudomonas putida GAM-1, which was able to grow in concentrations of CdCl2 as high as 7 mM, was isolated from soil in a rice paddy. This bacterium harbored a DNA plasmid of about 52 kilobases. The plasmid (pGU100) transformed Escherichia coli C600 to cadmium resistance. A cadmium-resistant transform...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.165.1.334-335.1986
更新日期:1986-01-01 00:00:00
abstract::Twenty-four Lactobacillus plantarum supermotifs (LPSMs) with lengths from approximately 800 to 1,000 nucleotides were identified in the L. plantarum genome. LPSMs were conserved in other L. plantarum strains but not in other species. Secondary structure analysis predicted that LPSMs may fold into cruciform-like struct...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01672-08
更新日期:2009-05-01 00:00:00
abstract::During the progression of Mycoplasma hominis cultures from the early logarithmic phase to the stationary phase of growth, the total phospholipid content of the cell membranes decreased. Measurement of the amount of the various phospholipids during the growth cycle showed that a decrease in the phosphatidylglycerol (PG...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.121.2.631-639.1975
更新日期:1975-02-01 00:00:00
abstract::The deduced amino acid sequences of the flagellins of Pseudomonas syringae pv. tabaci and P. syringae pv. glycinea are identical; however, their abilities to induce a hypersensitive reaction are clearly different. The reason for the difference seems to depend on the posttranslational modification of the flagellins. To...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.22.6658-6665.2003
更新日期:2003-11-01 00:00:00
abstract::The dermatophyte Trichophyton schoenleinii elaborates an extracellular collagenase which is specific for native collagen and gelatin and is separable from an enzyme which is active against Azocoll. The fungal enzyme differs from the bacterial collagenases in its acid pH and small molecular weight. In addition, the fun...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.95.1.43-46.1968
更新日期:1968-01-01 00:00:00
abstract::The mutation sup-3 in Bacillus subtilis suppresses ochre (TAA) mutations at each of three codons in the 5' end of the cat-86 coding sequence. The suppressor is shown to insert lysine at ochre codons. The efficiency of suppression by sup-3 is about 15%, as determined by changing a cat-86 Lys codon (codon 12) to an ochr...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.10.5322-5324.1989
更新日期:1989-10-01 00:00:00
abstract::To isolate strains with new recA mutations that differentially affect RecA protein functions, we mutagenized in vitro the recA gene carried by plasmid mini-F and then introduced the mini-F-recA plasmid into a delta recA host that was lysogenic for prophage phi 80 and carried a lac duplication. By scoring prophage indu...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.5.2415-2423.1989
更新日期:1989-05-01 00:00:00
abstract::Extracytoplasmic function σ factors that are stress inducible are often sequestered in an inactive complex with a membrane-associated anti-σ factor. Mycobacterium tuberculosis membrane-associated anti-σ factors have a small, stable RNA gene A (ssrA)-like degron for targeted proteolysis. Interaction between the unfolda...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00748-18
更新日期:2019-02-25 00:00:00
abstract::Mutants defective in uptake or utilization of a given metabolite can readily be obtained from facultative auxotrophs (for that metabolite) by penicillin enrichment under nonpermissive conditions in the presence of a low level of the required metabolite. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.121.3.1216-1218.1975
更新日期:1975-03-01 00:00:00
abstract::Various methods of inducing autolysis of Escherichia coli cells were investigated, some being described here for the first time. For the autolysis of growing cells only induction methods interfering with the biosynthesis of peptidoglycan were taken into consideration, whereas with harvested cells autolysis was induced...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:
更新日期:1982-10-01 00:00:00
abstract::The genes encoding ammonia monooxygenase (amoCAB), hydroxylamine oxidoreductase (hao), and the c-type cytochrome c-554 (hcy) are present in multiple copies in the genome of Nitrosomonas europaea. The upstream regions of the two copies of amoC, the three copies of hao, and one copy of hcy were cloned and sequenced. Pri...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.3.1096-1100.2001
更新日期:2001-02-01 00:00:00
abstract::Escherichia coli VC30 is a temperature-sensitive mutant which is defective in autolysis. Strain VC30 lyses at 30 degrees C when treated with beta-lactam antibiotics or D-cycloserine or when deprived of diaminiopimelic acid. The same treatments inhibit growth of the mutant at 42 degrees C but do not cause lysis. Strain...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.159.1.385-387.1984
更新日期:1984-07-01 00:00:00
abstract::The ompB operon encodes OmpR and EnvZ, two proteins that are necessary for the expression and osmoregulation of the OmpF and OmpC porins in Escherichia coli. We have used in vitro and in vivo experiments to show that cyclic AMP and the cyclic AMP receptor protein (CRP) directly regulate ompB. ompB expression in an omp...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.3.664-670.1992
更新日期:1992-02-01 00:00:00
abstract::Toxin-antitoxin (TA) systems have been reported in the genomes of most bacterial species, and their role when located on the chromosome is still debated. TA systems are particularly abundant in the massive cassette arrays associated with chromosomal superintegrons (SI). Here, we describe the characterization of two su...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01389-12
更新日期:2013-05-01 00:00:00
abstract:UNLABELLED:Deacetylation of 7-aminocephalosporanic acid (7-ACA) at position C-3 provides valuable starting material for producing semisynthetic β-lactam antibiotics. However, few enzymes have been characterized in this process before now. Comparative analysis of the genome of the thermophilic bacterium Alicyclobacillus...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00471-15
更新日期:2015-11-02 00:00:00
abstract::In Salmonella typhimurium the two enzymes of proline catabolism, proline oxidase and Delta(1)-pyrroline-5-carboxylic acid dehydrogenase, are subject to catabolite repression when the cells are grown in the presence of glucose. Mutants partially relieved of catabolite repression (PutR) for the proline catabolic enzymes...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.111.2.375-382.1972
更新日期:1972-08-01 00:00:00
abstract::Streptococcus agalactiae (group B streptococcus [GBS]) is a major cause of infections in newborns, pregnant women, and immunocompromised patients. GBS strain CNCTC10/84 is a clinical isolate that has high virulence in animal models of infection and has been used extensively to study GBS pathogenesis. Two unusual featu...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00504-20
更新日期:2020-11-04 00:00:00
abstract::We investigated the capacity of the hyperthermophile Pyrococcus furiosus for DNA repair by measuring survival at high levels of 60Co gamma-irradiation. The P. furiosus 2-Mb chromosome was fragmented into pieces ranging from 500 kb to shorter than 30 kb at a dose of 2,500 Gy and was fully restored upon incubation at 95...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.14.4643-4645.1997
更新日期:1997-07-01 00:00:00
abstract::We have previously shown that the TolA protein is required for the correct surface expression of the Escherichia coli O7 antigen lipopolysaccharide (LPS). In this work, delta tolA and delta pal mutants of E. coli K-12 W3110 were transformed with pMF19 (encoding a rhamnosyltransferase that reconstitutes the expression ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.10.3359-3368.2005
更新日期:2005-05-01 00:00:00
abstract::The antibiotic d-cycloserine is an effective inhibitor of alanine racemase. The lack of inhibition by l-cycloserine of alanine racemase from Staphylococcus aureus led Roze and Strominger to formulate the cycloserine hypothesis. This hypothesis states that d-cycloserine has the conformation required of the substrates o...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.110.3.978-987.1972
更新日期:1972-06-01 00:00:00
abstract::A fusion between lacZ and ftsZ of Escherichia coli was constructed to obtain a beta-galactosidase-FtsZ fusion protein. This fusion protein was used to raise antibodies against cell division protein FtsZ. Six monoclonal antibodies were obtained, and they reacted with FtsZ from cytoplasm and membrane fractions. The epit...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.7.1886-1893.1994
更新日期:1994-04-01 00:00:00
abstract::The frizzy (frz) genes of Myxococcus xanthus are required to control directed motility during vegetative growth and fruiting body formation. FrzCD, a protein homologous to the methyl-accepting chemotaxis proteins from enteric bacteria, is modified by methylation in response to environmental conditions. Transfer of cel...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.15.4936-4940.1993
更新日期:1993-08-01 00:00:00
abstract::A delta tyrT::kan mutant from Escherichia coli K-12 (DTK-12) shows a transient growth lag that is caused by glycine starvation (U. Michelsen, M. Bösl, T. Dingermann, and H. Kersten, J. Bacteriol. 171:5987-5994, 1989). The same deletion, transduced into the relA1 spoT1 mutant CA274 to construct strain DTC274, caused co...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.1.221-231.1994
更新日期:1994-01-01 00:00:00
abstract::Postembedding staining of intracellular carbohydrates on thin sections of Staphylococcus aureus was studied by the silver methenamine and the wheat germ agglutinin-gold techniques. Staining of silver grains was observed on both the cell wall and the cross wall. The staining was interpreted to be due to teichoic acid. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.3.1358-1362.1987
更新日期:1987-03-01 00:00:00
abstract::A spontaneously arising regulatory mutant of the gluconate system in Escherichia coli was isolated. This mutant became constitutive, probably in one step, for gluconate high-affinity transport, gluconokinase, and gluconate-6-P dehydrase. The mutation involved (gntR18) is cotransducible with asd. Pseudorevertants, deri...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.114.2.469-473.1973
更新日期:1973-05-01 00:00:00
abstract::The peptidoglycan from two types of filamentous cell division mutants of Agmenellum quadruplicatum strain BG1 has been compared to that of the parent organism. Small variations in the total peptidoglycan composition on a dry-weight basis were found in the mutants. The reduced level of peptidoglycan in the serpentine m...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.111.2.614-621.1972
更新日期:1972-08-01 00:00:00
abstract::To identify the genes in coliphage 186 that are required for lysogeny, we isolated clear-plaque mutants. Complementation studies and DNA sequencing identified two genes, the cI gene for the immunity maintenance repressor and the cII gene, which is required only for the establishment of lysogeny. One mutant carried a c...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.16.5286-5288.1993
更新日期:1993-08-01 00:00:00
abstract::If bacteria are incapable of forming and incorporating proteins into the cytoplasmic membranes in all phases of the cell cycle, then not all cells from an asynchronous culture should be capable of growth when switched to a new carbon and energy source whose metabolism requires new membrane function. The transfer of an...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.124.1.435-444.1975
更新日期:1975-10-01 00:00:00