Promoters recognized by Escherichia coli RNA polymerase selected by function: highly efficient promoters from bacteriophage T5.

abstract：:The anaerobic spore former Clostridioides difficile causes significant diarrheal disease in humans and other mammals. Infection begins with the ingestion of dormant spores, which subsequently germinate within the host gastrointestinal tract. There, the vegetative cells proliferate and secrete two exotoxins, TcdA and T...

journal_title：Journal of bacteriology

authors： Edwards AN,Krall EG,McBride SM

更新日期：2020-01-02 00:00:00

abstract：:Avian-pathogenic Escherichia coli (APEC) strains cause extraintestinal diseases in avian species. Here, we present the draft genome of an APEC strain (SCI-07) from Brazil that was isolated from skin lesions (gelatinous edema) on the head and periorbital tissues of a laying hen with swollen head syndrome. ...

journal_title：Journal of bacteriology

authors： Rojas TC,Parizzi LP,Tiba MR,Chen L,Pereira GA,Sangal V,Yang J,Yu J,Dias da Silveira W

更新日期：2012-06-01 00:00:00

abstract：:The first generalized transducing bacteriophage reported for Bacillus megaterium has been characterized. Optimum conditions for lysate production and transduction procedures were established so that transducing frequencies of 8 x 10(-6) and higher are now possible. The phage, MP13, has a head diameter of 97 nm and a c...

journal_title：Journal of bacteriology

authors： Vary PS,Garbe JC,Franzen M,Frampton EW

更新日期：1982-03-01 00:00:00

abstract：:Members of the pPT23A plasmid family of Pseudomonas syringae play an important role in the interaction of this bacterial pathogen with host plants. Complete sequence analysis of several pPT23A family plasmids (PFPs) has provided a glimpse of the gene content and virulence function of these plasmids. We constructed a m...

journal_title：Journal of bacteriology

authors： Zhao Y,Ma Z,Sundin GW

更新日期：2005-03-01 00:00:00

abstract：UNLABELLED:Cell shape has been suggested to play an important role in the regulation of bacterial attachment to surfaces and the formation of communities associated with surfaces. We found that a cardiolipin synthase (Δcls) mutant of the rod-shaped bacterium Rhodobacter sphaeroides--in which synthesis of the anionic, h...

journal_title：Journal of bacteriology

authors： Lin TY,Santos TM,Kontur WS,Donohue TJ,Weibel DB

更新日期：2015-11-01 00:00:00

abstract：:The multifunctional 6-methylsalicylic acid synthase gene from Penicillium patulum was engineered for regulated expression in Streptomyces coelicolor. Production of significant amounts of 6-methylsalicylic acid by the recombinant strain was proven by nuclear magnetic resonance spectroscopy. These results suggest that i...

journal_title：Journal of bacteriology

authors： Bedford DJ,Schweizer E,Hopwood DA,Khosla C

更新日期：1995-08-01 00:00:00

abstract：:Harper, M. K. (U.S. Department of Agriculture, Washington, D.C.), H. R. Curran, and M. J. Pallansch. Properties of electrodialyzed bacterial spores. J. Bacteriol. 88:1338-1340. 1964.-Washed spores of Bacillus cereus, B. megaterium, and B. stearothermophilis suspended in distilled water were electrodialyzed at a potent...

journal_title：Journal of bacteriology

authors： HARPER MK,CURRAN HR,PALLANSCH MJ

更新日期：1964-11-01 00:00:00

abstract：:The effect of CheY and fumarate on switching frequency and rotational bias of the bacterial flagellar motor was analyzed by computer-aided tracking of tethered Escherichia coli. Plots of cells overexpressing CheY in a gutted background showed a bell-shaped correlation curve of Switching frequency and bias centering at...

journal_title：Journal of bacteriology

authors： Montrone M,Eisenbach M,Oesterhelt D,Marwan W

更新日期：1998-07-01 00:00:00

abstract：:Methanol dehydrogenase, the enzyme that oxidizes methanol to formaldehyde in gram-negative methylotrophs, contains the prosthetic group pyrroloquinoline quinone (PQQ). To begin to analyze how the synthesis of PQQ is coordinated with the production of other methanol dehydrogenase components, the transcription of one of...

journal_title：Journal of bacteriology

authors： Ramamoorthi R,Lidstrom ME

更新日期：1995-01-01 00:00:00

abstract：:We screened Salmonella typhimurium, Citrobacter freundii, Klebsiella pneumoniae, Shigella boydii, and many isolates of Escherichia coli for DNA sequences homologous to those encoding each of two unrelated type I restriction and modification systems (EcoK and EcoA). Both K- and A-related hsd genes were identified, but ...

journal_title：Journal of bacteriology

authors： Daniel AS,Fuller-Pace FV,Legge DM,Murray NE

更新日期：1988-04-01 00:00:00

abstract：:ICE6013 represents one of two families of integrative conjugative elements (ICEs) identified in the pan-genome of the human and animal pathogen Staphylococcus aureus Here we investigated the excision and conjugation functions of ICE6013 and further characterized the diversity of this element. ICE6013 excision was not ...

journal_title：Journal of bacteriology

authors： Sansevere EA,Luo X,Park JY,Yoon S,Seo KS,Robinson DA

更新日期：2017-03-28 00:00:00

abstract：:A sequenced collection of plasmid-borne random fusions of Escherichia coli DNA to a Photorhabdus luminescens luxCDABE reporter was used as a starting point to select a set of 689 nonredundant functional gene fusions. This group, called LuxArray 1.0, represented 27% of the predicted transcriptional units in E. coli. Hi...

journal_title：Journal of bacteriology

authors： Van Dyk TK,DeRose EJ,Gonye GE

更新日期：2001-10-01 00:00:00

abstract：:The phoP-phoQ-regulated pagC locus is essential for full virulence and survival within macrophages of Salmonella typhimurium. The protein product, DNA sequence, and transcript of pagC were determined. The pagC locus encodes a single 188-amino-acid membrane protein that is similar to the ail-encoded eucaryotic cell inv...

journal_title：Journal of bacteriology

authors： Pulkkinen WS,Miller SI

更新日期：1991-01-01 00:00:00

abstract：:We constructed a shuttle vector, pE5-2, which can replicate in both Bacteroides spp. and Escherichia coli. pE5-2 contains a cryptic Bacteroides plasmid (pB8-51), a 3.8-kilobase (kb) EcoRI-D fragment from the 41-kb Bacteroides fragilis plasmid pBF4, and RSF1010, an IncQ E. coli plasmid. pE5-2 was mobilized by R751, an ...

journal_title：Journal of bacteriology

authors： Shoemaker NB,Guthrie EP,Salyers AA,Gardner JF

更新日期：1985-05-01 00:00:00

abstract：:The mechanisms protecting nitrogenase in Gluconacetobacter diazotrophicus from damage by oxygen were studied. Evidence is provided suggesting that in G. diazotrophicus these mechanisms include respiratory protection as well as conformational protection in which a putative FeSII Shethna protein is involved. ...

journal_title：Journal of bacteriology

authors： Ureta A,Nordlund S

更新日期：2002-10-01 00:00:00

abstract：:In gram-negative organisms, enzymes belonging to the low-molecular-weight protein tyrosine phosphatase (LMPTP) family are involved in the regulation of important physiological functions, including stress resistance and synthesis of the polysaccharide capsule. LMPTPs have been identified also in gram-positive bacteria,...

journal_title：Journal of bacteriology

authors： Musumeci L,Bongiorni C,Tautz L,Edwards RA,Osterman A,Perego M,Mustelin T,Bottini N

更新日期：2005-07-01 00:00:00

abstract：:Branching enzyme (BE) catalyzes formation of the branch points in glycogen and amylopectin by cleavage of the alpha-1,4 linkage and its subsequent transfer to the alpha-1,6 position. We have identified a novel BE encoded by an uncharacterized open reading frame (TK1436) of the hyperthermophilic archaeon Thermococcus k...

journal_title：Journal of bacteriology

authors： Murakami T,Kanai T,Takata H,Kuriki T,Imanaka T

更新日期：2006-08-01 00:00:00

abstract：:Bacterial pathogenesis depends on changes in metabolic and virulence gene expression in response to changes within a pathogen's environment. The plague-causing pathogen, Yersinia pestis, requires expression of the gene encoding the Pla protease for progression of pneumonic plague. The catabolite repressor protein Crp,...

journal_title：Journal of bacteriology

authors： Ritzert JT,Lathem WW

更新日期：2018-05-09 00:00:00

abstract：:Biodegradation of metal-citrate complexes by Pseudomonas fluorescens depends on the nature of the complex formed between the metal and citric acid. Bidentate Fe(III)-, Ni-, and Zn-citrate complexes were readily biodegraded, but the tridentate Cd- and Cu-citrate, and U-citrate complexes were not. The biodegradation of ...

journal_title：Journal of bacteriology

authors： Joshi-Tope G,Francis AJ

更新日期：1995-04-01 00:00:00

abstract：:The major regulator controlling the physiological switch between aerobic and anaerobic growth conditions in Escherichia coli is the DNA binding protein FNR. To identify genes controlled by FNR, we used Affymetrix Antisense GeneChips to compare global gene expression profiles from isogenic MG1655 wild-type and Deltafnr...

journal_title：Journal of bacteriology

authors： Kang Y,Weber KD,Qiu Y,Kiley PJ,Blattner FR

更新日期：2005-02-01 00:00:00

abstract：:The biosynthesis of asparaginase II in Saccharomyces cerevisiae is subject to nitrogen catabolite repression. In the present study we examined the physiological effects of glutamate auxotrophy on cellular metabolism and on the nitrogen catabolite repression of asparaginase II. Glutamate auxotrophic cells, incubated wi...

journal_title：Journal of bacteriology

authors： Kang L,Keeler ML,Dunlop PC,Roon RJ

更新日期：1982-07-01 00:00:00

abstract：:Small subunit rDNA sequences were determined for 20 species of the genera Acetogenium, Clostridium, Thermoanaerobacter, Thermoanaerobacterium, Thermoanaerobium, and Thermobacteroides, 3 non-validly described species, and 5 isolates of anaerobic thermophilic bacteria, providing a basis for a phylogenetic analysis of th...

journal_title：Journal of bacteriology

authors： Rainey FA,Ward NL,Morgan HW,Toalster R,Stackebrandt E

更新日期：1993-08-01 00:00:00

abstract：:The opportunistic pathogen Pseudomonas aeruginosa produces multifunctional, polar, filamentous appendages termed type IV pili. Type IV pili are involved in colonization during infection, twitching motility, biofilm formation, bacteriophage infection, and natural transformation. Electrostatic surface analysis of modele...

journal_title：Journal of bacteriology

authors： van Schaik EJ,Giltner CL,Audette GF,Keizer DW,Bautista DL,Slupsky CM,Sykes BD,Irvin RT

更新日期：2005-02-01 00:00:00

abstract：:Chlamydia trachomatis is a human bacterial pathogen that multiplies only within an intracellular membrane-bound vacuole, the inclusion. C. trachomatis includes ocular and urogenital strains, usually causing infections restricted to epithelial cells of the conjunctiva and genital mucosa, respectively, and lymphogranulo...

journal_title：Journal of bacteriology

authors： Almeida F,Borges V,Ferreira R,Borrego MJ,Gomes JP,Mota LJ

更新日期：2012-12-01 00:00:00

abstract：:Hyaluronate stimulated the formation of exolipase by Serratia marcescens. This ability was abolished when all carboxyl groups of hyaluronate were methyl esterified. Additional studies suggested that the biological inactivity of esterified hyaluronate should be ascribed to the reduced conformational order of the molecu...

journal_title：Journal of bacteriology

authors： Jäger KE,Winkler UK

更新日期：1979-09-01 00:00:00

abstract：:Several mycobacterial strains, such as Mycobacterium flavescens, Mycobacterium gastri, Mycobacterium neoaurum, Mycobacterium parafortuitum, Mycobacterium peregrinum, Mycobacterium phlei, Mycobacterium smegmatis, Mycobacterium tuberculosis, and Mycobacterium vaccae, were found to grow on carbon monoxide (CO) as the sol...

journal_title：Journal of bacteriology

authors： Park SW,Hwang EH,Park H,Kim JA,Heo J,Lee KH,Song T,Kim E,Ro YT,Kim SW,Kim YM

更新日期：2003-01-01 00:00:00

abstract：:Pertussis toxin (PT), the major virulence factor of Bordetella pertussis, is composed of five different subunits whose genes are organized as an operon. We report the mapping of the promoter region of the PT operon and show that this promoter is only weakly active in Escherichia coli. Bordetella parapertussis and Bord...

journal_title：Journal of bacteriology

authors： Nicosia A,Rappuoli R

更新日期：1987-06-01 00:00:00

abstract：:Biofilm formation in Staphylococcus epidermidis is dependent upon the ica operon-encoded polysaccharide intercellular adhesin, which is subject to phase-variable and environmental regulation. The icaR gene, located adjacent to the ica operon, appears to be a member of the tetR family of transcriptional regulators. In ...

journal_title：Journal of bacteriology

authors： Conlon KM,Humphreys H,O'Gara JP

更新日期：2002-08-01 00:00:00

abstract：:In stationary phase, 95% of the fructosyltransferase (FTase) activity of Streptococcus salivarius ATCC 25975 was found associated with the cells. Within the first 15 min after inoculation into fresh medium, the specific activity of cell-associated FTase decreased by 92% of its initial value. After this period of initi...

journal_title：Journal of bacteriology

authors： Jacques NA,Wittenberger CL

更新日期：1981-12-01 00:00:00

abstract：:The direction of chromosome replication in a temperature-sensitive initiation mutant of Escherichia coli (CT28) is shown autoradiographically to be bidirectional. This mode of replication persists even when the rate of replication is reduced by slow growth in succinate minimal medium or in the presence of chlorampheni...

journal_title：Journal of bacteriology

authors： Rodriguez RL,Davern CI

更新日期：1976-01-01 00:00:00