Expression of a functional fungal polyketide synthase in the bacterium Streptomyces coelicolor A3(2).

abstract：:The lethal and mutagenic effects of various mutagens on Neisseria gonorrhoeae were investigated. Lethality studies demonstrated that N. gonorrhoeae was relatively sensitive to ethyl methanesulfonate, UV light, and methyl methanesulfonate. Although N. gonorrhoeae was readily mutated by ethyl methanesulfonate and N-meth...

journal_title：Journal of bacteriology

authors： Campbell LA,Yasbin RE

更新日期：1984-10-01 00:00:00

abstract：:S. Sawada and co-workers reported that a monoclonal antibody (MAb), E87, interacted with about 80% of Pseudomonas aeruginosa isolates, and they separated a rhamnose-rich polysaccharide as the probable antigen for MAb E87 from P. aeruginosa IFO 3080 (S. Sawada, T. Kawamura, Y. Masuho, and K. Tomibe, J. Infec. Dis. 152:...

journal_title：Journal of bacteriology

authors： Yokota S,Kaya S,Araki Y,Ito E,Kawamura T,Sawada S

更新日期：1990-10-01 00:00:00

abstract：:Cleared lysates of 13 species of filamentous cyanobacteria were examined for the presence of extrachromosomal DNA by using agarose gel electrophoresis and ethidium bromide staining. Seven of the 13 species contained extrachromosomal covalently closed circular DNA, and all but 1 species contained multiple elements. The...

journal_title：Journal of bacteriology

authors： Simon RD

更新日期：1978-10-01 00:00:00

abstract：:Bacteriophage T7 RNA polymerase is stable in Escherichia coli but very susceptible to cleavage by at least one endoprotease after cell lysis. The major source of this endoprotease activity was found to be localized to the outer membrane of the cell. A rapid whole-cell assay was developed to screen different strains fo...

journal_title：Journal of bacteriology

authors： Grodberg J,Dunn JJ

更新日期：1988-03-01 00:00:00

abstract：:A new Salmonella enterica serovar Typhimurium strain has been constructed to facilitate tightly regulated gene expression. Arabinose-inducible and glucose-repressible expression of a T7 RNA polymerase gene that has been integrated with an adjacent araC-P(BAD) control element into the bacterial chromosome allows dynami...

journal_title：Journal of bacteriology

authors： McKinney J,Guerrier-Takada C,Galán J,Altman S

更新日期：2002-11-01 00:00:00

abstract：:Undomesticated strains of Bacillus subtilis exhibit extensive colony spreading on certain soft agarose media: first the formation of dendritic clusters of cells, followed by spreading (pellicle-like) growth to cover the entire surface. These phases of colonization are dependent on the level of potassium ion (K(+)) but...

journal_title：Journal of bacteriology

authors： Kinsinger RF,Kearns DB,Hale M,Fall R

更新日期：2005-12-01 00:00:00

abstract：:In vitro, archaeal SRP54 binds SRP RNA in the absence of SRP19, suggesting the latter to be expendable in Archaea. Accordingly, the Haloferax volcanii SRP19 gene was deleted. Although normally transcribed at a level comparable to that of the essential SRP54 gene, SRP19 deletion had no effect on cell growth, membrane p...

journal_title：Journal of bacteriology

authors： Yurist S,Dahan I,Eichler J

更新日期：2007-01-01 00:00:00

abstract：:Cecropins are a family of small, basic antibacterial polypeptides which can be isolated from pupae of immunized Lepidoptera. They are active against both gram-negative and gram-positive bacteria. We studied a mutant of Escherichia coli, strain SB1004, which is more sensitive to cecropin D than is the parental strain. ...

journal_title：Journal of bacteriology

authors： Sidén I,Boman HG

更新日期：1983-04-01 00:00:00

abstract：:Wallis, Craig (Baylor University College of Medicine, Houston, Tex.), Joseph L. Melnick, and Fred Rapp. Effects of pancreatin on the growth of reovirus. J. Bacteriol. 92:155-160. 1966.-The influence of pancreatin and other proteolytic enzymes on the growth, plaque formation, and antigenicity of reovirus was studied. S...

journal_title：Journal of bacteriology

authors： Wallis C,Melnick JL,Rapp F

更新日期：1966-07-01 00:00:00

abstract：:The umuDC gene products, whose expression is induced by DNA-damaging treatments, have been extensively characterized for their role in SOS mutagenesis. We have recently presented evidence that supports a role for the umuDC gene products in the regulation of growth after DNA damage in exponentially growing cells, analo...

journal_title：Journal of bacteriology

authors： Murli S,Opperman T,Smith BT,Walker GC

更新日期：2000-02-01 00:00:00

abstract：:Individually, the ribosomal proteins L1, L23, L36, and S6 are not essential for cell proliferation of Bacillus subtilis, but the absence of any one of these ribosomal proteins causes a defect in the formation of the 70S ribosomes and a reduced growth rate. In mutant strains individually lacking these ribosomal protein...

journal_title：Journal of bacteriology

authors： Akanuma G,Yamazaki K,Yagishi Y,Iizuka Y,Ishizuka M,Kawamura F,Kato-Yamada Y

更新日期：2018-08-24 00:00:00

abstract：:Impurities believed to be polysaccharides have been found in mycobacterial deoxyribonucleic acid (DNA) preparations. Agar-gel diffusion of the DNA preparations against concanavalin A indicated the presence of three polysaccharides and was used to follow the purification procedures. The polysaccharides appeared to be t...

journal_title：Journal of bacteriology

authors： Hill EB,Wayne LG,Gross WM

更新日期：1972-12-01 00:00:00

abstract：:Antigen 43 has been identified as a unique protein complex in the outer membrane of Escherichia coli. The complex contains two different polypeptides, alpha (Mr, 60,000) and beta (Mr, 53,000), in equal stoichiometry (P. Owen, P. Caffrey, and L.-G. Josefsson, J. Bacteriol. 169:3770-3777, 1987). The alpha subunit was re...

journal_title：Journal of bacteriology

authors： Caffrey P,Owen P

更新日期：1989-07-01 00:00:00

abstract：:Chlamydial lipopolysaccharide (LPS) agglutinated mouse and rabbit erythrocytes but not human, guinea pig, or pronghorn antelope erythrocytes. Hemagglutination was not specific for Chlamydia spp., as rough LPSs from Coxiella burnetii and Escherichia coli also agglutinated erythrocytes from the same animal species. Nona...

journal_title：Journal of bacteriology

authors： Watkins NG,Caldwell HD,Hackstadt T

更新日期：1987-08-01 00:00:00

abstract：:Whole cells of Lactobacillus acidophilus are capable of degrading thymidine to thymine, suggesting the presence of thymidine phosphorylase (or thymidine hydrolase). This activity was also demonstrated in cell-free extracts. ...

journal_title：Journal of bacteriology

authors： Sawula RV,Zamenhof S,Zamenhof PJ

更新日期：1974-03-01 00:00:00

abstract：:Type III secretion systems (T3SSs) secrete needle components, pore-forming translocators, and the translocated effectors. In part, effector recognition by a T3SS involves their N-terminal amino acids and their 5' mRNA. To investigate whether similar molecular constraints influence translocator secretion, we scrutinize...

journal_title：Journal of bacteriology

authors： Amer AA,Åhlund MK,Bröms JE,Forsberg Å,Francis MS

更新日期：2011-12-01 00:00:00

abstract：:The Vibrio fischeri luminescence (lux) genes are regulated by the 250-amino-acid-residue LuxR protein and a V. fischeri metabolite termed autoinducer. The V. fischeri lux regulon consists of two divergently transcribed units. Autoinducer and LuxR activate transcription of the luxICDABE operon and autoregulate the luxR...

journal_title：Journal of bacteriology

authors： Choi SH,Greenberg EP

更新日期：1992-06-01 00:00:00

abstract：:The protein SpoIVB plays a key role in signaling in the final sigma(K) checkpoint of Bacillus subtilis. This regulatory mechanism coordinates late gene expression during development in this organism and we have recently shown SpoIVB to be a serine peptidase. SpoIVB signals by transiting a membrane, undergoing self-cle...

journal_title：Journal of bacteriology

authors： Hoa NT,Brannigan JA,Cutting SM

更新日期：2002-01-01 00:00:00

abstract：:The Brucella abortus virB locus contains 12 open reading frames, termed virB1 through virB12, which encode a type IV secretion system. Polar mutations in the virB locus markedly reduce the ability of B. abortus to survive in cultured macrophages or to persist in organs of mice. While a nonpolar deletion of the virB2 g...

journal_title：Journal of bacteriology

authors： den Hartigh AB,Rolán HG,de Jong MF,Tsolis RM

更新日期：2008-07-01 00:00:00

abstract：:Xylella fastidiosa is a xylem-limited nonflagellated bacterium that causes economically important diseases of plants by developing biofilms that block xylem sap flow. How the bacterium is translocated downward in the host plant's vascular system against the direction of the transpiration stream has long been a puzzlin...

journal_title：Journal of bacteriology

authors： Meng Y,Li Y,Galvani CD,Hao G,Turner JN,Burr TJ,Hoch HC

更新日期：2005-08-01 00:00:00

abstract：:All Escherichia coli strains so far examined possess a chromosomally encoded nanATEK-yhcH operon for the catabolism of sialic acids. These unique nine-carbon sugars are synthesized primarily by higher eukaryotes and can be used as carbon, nitrogen, and energy sources by a variety of microbial pathogens or commensals. ...

journal_title：Journal of bacteriology

authors： Kalivoda KA,Steenbergen SM,Vimr ER,Plumbridge J

更新日期：2003-08-01 00:00:00

abstract：:Streptococcus bovis JB1 cells energized with glucose transported glutamine at a rate of 7 nmol/mg of protein per min at a pH of 5.0 to 7.5; sodium had little effect on the transport rate. Because valinomycin-treated cells loaded with K and diluted into Na (pH 6.5) to create an artificial delta psi took up little gluta...

journal_title：Journal of bacteriology

authors： Chen GJ,Russell JB

更新日期：1989-06-01 00:00:00

abstract：:Coxiella burnetii, the etiological agent of acute and chronic Q fever in humans, is a naturally intracellular pathogen that directs the formation of an acidic Coxiella-containing vacuole (CCV) derived from the host lysosomal network. Central to its pathogenesis is a specialized type IVB secretion system (T4SS) that de...

journal_title：Journal of bacteriology

authors： Weber MM,Chen C,Rowin K,Mertens K,Galvan G,Zhi H,Dealing CM,Roman VA,Banga S,Tan Y,Luo ZQ,Samuel JE

更新日期：2013-09-01 00:00:00

abstract：:Plasmid ColIb-P9 of the I alpha incompatibility group is known to encode a DNA primase that acts in the conjugal transfer of the plasmid and can substitute for mutant dnaG gene product in vegetative replication of the Escherichia coli chromosome. The relevant genetic determinant (sog) has previously been cloned into a...

journal_title：Journal of bacteriology

authors： Dalrymple BP,Boulnois GJ,Wilkins BM,Orr E,Williams PH

更新日期：1982-07-01 00:00:00

abstract：:The exosporium of Bacillus anthracis is comprised of two distinct layers: a basal layer and a hair-like nap that covers the basal layer. The hair-like nap contains the glycoproteins BclA and, most likely, BclB. BclA and BclB are directed to assemble into the exosporium by motifs in their N-terminal domains. Here, we i...

journal_title：Journal of bacteriology

authors： Thompson BM,Hoelscher BC,Driks A,Stewart GC

更新日期：2011-10-01 00:00:00

abstract：:Here we present the genome sequence of Rhizobium grahamii CCGE502. R. grahamii groups with other newly described broad-host-range species, which are not very efficient Phaseolus vulgaris symbionts, with a wide geographic distribution and which constitutes a novel Rhizobium clade. ...

journal_title：Journal of bacteriology

authors： Althabegoiti MJ,Lozano L,Torres-Tejerizo G,Ormeño-Orrillo E,Rogel MA,González V,Martínez-Romero E

更新日期：2012-12-01 00:00:00

abstract：:Netropsin, a polypeptide antibiotic which binds specifically to adenylate-thymidylate-rich regions of deoxyribonucleic acid, inhibitis sporulation at about stage II, but does not inhibit growth of Bacillys subtilis. An analysis of the sporulation-associated enzymes aconitase, alkaline phosphatase, and glucose dehydrog...

journal_title：Journal of bacteriology

authors： Keilman GR,Brutis K,Tanimoto B,Doi RH

更新日期：1976-10-01 00:00:00

abstract：:The structural gene coding for the delta 5-3-ketosteroid isomerase (KSI) of Pseudomonas putida biotype B has been cloned, and its entire nucleotide sequence has been determined by a dideoxynucleotide chain termination method. A 2.1-kb DNA fragment containing the ksi gene was cloned from a P. putida biotype B genomic l...

journal_title：Journal of bacteriology

authors： Kim SW,Kim CY,Benisek WF,Choi KY

更新日期：1994-11-01 00:00:00

abstract：:The group A streptococcus (GAS), Streptococcus pyogenes, is an important human pathogen that causes infections ranging in severity from self-limiting pharyngitis to severe invasive diseases that are associated with significant morbidity and mortality. The pathogenic effects of GAS are mediated by the expression of vir...

journal_title：Journal of bacteriology

authors： Gusa AA,Gao J,Stringer V,Churchward G,Scott JR

更新日期：2006-07-01 00:00:00

abstract：:The occurrence of class II restriction endonucleases (enzymes that both recognize and cleave a specific nucleotide sequence in deoxyribonucleic acid [DNA] in Staphylococcus aureus has been investigated by analysis of crude extracts obtained from different propagating strains of the International Phage Typing System. O...

journal_title：Journal of bacteriology

authors： Stobberingh EE,Schiphof R,Sussenbach JS

更新日期：1977-08-01 00:00:00