Effects of homocitrate, homocitrate lactone, and fluorohomocitrate on nitrogenase in NifV- mutants of Azotobacter vinelandii.

Abstract:

:Azotobacter vinelandii DJ71, which contains a mutation in the nifV gene, was derepressed for nitrogenase in the presence of homocitrate. When dinitrogenase was isolated from this culture, it was found to be identical to the wild-type dinitrogenase. However, when the same NifV- strain was derepressed in the presence of erythrofluorohomocitrate, a homocitrate analog which produces a nitrogenase with wild-type properties in vitro, the isolated dinitrogenase was characteristic of the NifV- enzyme. These data show that homocitrate, but not fluorohomocitrate, is utilized by NifV- mutant cells. Fluorohomocitrate does not inhibit the uptake of homocitrate because the wild-type phenotype resulted when both compounds were added to the medium during nitrogenase derepression. Homocitrate lactone failed to cure the NifV- phenotype.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Madden MS,Paustian TD,Ludden PW,Shah VK

doi

10.1128/jb.173.17.5403-5405.1991

subject

Has Abstract

pub_date

1991-09-01 00:00:00

pages

5403-5

issue

17

eissn

0021-9193

issn

1098-5530

journal_volume

173

pub_type

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