Properties of Bacillus megaterium temperature-sensitive germination mutants.

abstract：:HypA and HypB are maturation proteins required for incorporation of nickel into the hydrogenase large subunit. To examine the functions of these proteins in nickel insertion, the hybF gene, which is a homolog of hypA essential for maturation of hydrogenases 1 and 2 from Escherichia coli, was overexpressed, and the pro...

journal_title：Journal of bacteriology

authors： Blokesch M,Rohrmoser M,Rode S,Böck A

更新日期：2004-05-01 00:00:00

abstract：:Bacillus subtilis strains carrying div-341 or sacU mutations, or both, have been characterized to reveal the roles of both genes in the initiation of sporulation, as well as in cell division and exoenzyme secretion. Both mutations were closely linked by transformation and caused the pleiotropic effects on sporulation ...

journal_title：Journal of bacteriology

authors： Sadaie Y,Kada T

更新日期：1985-08-01 00:00:00

abstract：:Microcin J25 (MccJ25) is a small peptide antibiotic produced by an Escherichia coli strain isolated from human feces. The genetic determinants for MccJ25 synthesis and immunity have been cloned from the low-copy-number wild-type plasmid pTUC1OO into the compatible vectors pBR322 and pACYC184. Physical and phenotypical...

journal_title：Journal of bacteriology

authors： Solbiati JO,Ciaccio M,Farías RN,Salomón RA

更新日期：1996-06-01 00:00:00

abstract：:Cellular vanadium metabolism was studied in Saccharomyces cerevisiae by isolating and characterizing vanadate [VO4(3-), V(V)]-resistant mutants. Vanadate growth inhibition was reversed by the removal of the vanadate from the medium, and vanadate resistance was found to be a recessive trait. Vanadate-resistant mutants ...

journal_title：Journal of bacteriology

authors： Willsky GR,Leung JO,Offermann PV Jr,Plotnick EK,Dosch SF

更新日期：1985-11-01 00:00:00

abstract：:We report the complete and annotated genome sequence of the nonpathogenic Listeria seeligeri SLCC3954 serovar 1/2b type strain harboring the smallest completely sequenced genome of the genus Listeria. ...

journal_title：Journal of bacteriology

authors： Steinweg C,Kuenne CT,Billion A,Mraheil MA,Domann E,Ghai R,Barbuddhe SB,Kärst U,Goesmann A,Pühler A,Weisshaar B,Wehland J,Lampidis R,Kreft J,Goebel W,Chakraborty T,Hain T

更新日期：2010-03-01 00:00:00

abstract：:In Pseudomonas aeruginosa, the CbrA/CbrB two-component system is instrumental in the maintenance of the carbon-nitrogen balance and for growth on carbon sources that are energetically less favorable than the preferred dicarboxylate substrates. The CbrA/CbrB system drives the expression of the small RNA CrcZ, which ant...

journal_title：Journal of bacteriology

authors： Abdou L,Chou HT,Haas D,Lu CD

更新日期：2011-06-01 00:00:00

abstract：:Sialidase activity varies widely among strains and tends to correlate with strain virulence in the avian pathogen Mycoplasma synoviae. To characterize the forms of selection acting on enzymes required for sialic acid scavenging and catabolism, the ratios of nonsynonymous (K(a)) to synonymous (K(s)) mutation frequency ...

journal_title：Journal of bacteriology

authors： May M,Brown DR

更新日期：2009-06-01 00:00:00

abstract：:The AraC homolog ToxT coordinately regulates virulence gene expression in Vibrio cholerae. ToxT is required for transcriptional activation of the genes encoding cholera toxin and the toxin coregulated pilus, among others. In this work we focused on the interaction of ToxT with the tcpA promoter and investigated the me...

journal_title：Journal of bacteriology

authors： Hulbert RR,Taylor RK

更新日期：2002-10-01 00:00:00

abstract：:The plasmid-encoded citrate determinant of the Lactococcus lactis subsp. lactis var. diacetylactis NCDO176 was cloned and functionally expressed in a Cit- Escherichia coli K-12 strain. From deletion derivative analysis, a 3.4-kilobase region was identified which encodes the ability to transport citrate. Analysis of pr...

journal_title：Journal of bacteriology

authors： David S,van der Rest ME,Driessen AJ,Simons G,de Vos WM

更新日期：1990-10-01 00:00:00

abstract：:A novel method is described that allows a direct determination of the location of a portion of the dipicolinic acid (DPA) in spores. Ultraviolet (UV) irradiation is shown to cause cross-linkage of DPA to spore proteins which have the characteristics of membrane proteins. We suggest that DPA resides in the inner foresp...

journal_title：Journal of bacteriology

authors： Germaine GR,Murrell WG

更新日期：1974-04-01 00:00:00

abstract：:A set of chemotaxis mutants of Bacillus subtilis was complemented by using SP beta c2 transducing bacteriophage either containing cloned segments of DNA or derived from abnormal excision of SP beta c2 dl2::Tn917 inserted into the chemotaxis region. Representative mutants were characterized in capillary assays for chem...

journal_title：Journal of bacteriology

authors： Ordal GW,Parker HM,Kirby JR

更新日期：1985-11-01 00:00:00

abstract：:An abundant 19.3-kDa Helicobacter pylori protein has been cloned, and the sequence is homologous with a ferritin-like protein produced by Escherichia coli K-12. Homologies are also present with a number of eucaryotic ferritins, as well as with the heme group-containing bacterioferritins. All amino acids involved in ch...

journal_title：Journal of bacteriology

authors： Frazier BA,Pfeifer JD,Russell DG,Falk P,Olsén AN,Hammar M,Westblom TU,Normark SJ

更新日期：1993-02-01 00:00:00

abstract：:Specific labeling of a single row of cellulose-synthesizing complexes (terminal complexes, TC subunits, TCs, or TC arrays) in Acetobacter xylinum by antibodies raised against a 93-kDa protein (the cyclic dignanylic acid-binding protein) has been demonstrated by using the sodium dodecyl sulfate (SDS)-freeze-fracture la...

journal_title：Journal of bacteriology

authors： Kimura S,Chen HP,Saxena IM,Brown RM Jr,Itoh T

更新日期：2001-10-01 00:00:00

abstract：:Transcription of the dnaA gene of the cyanobacterium Synechocystis sp. strain PCC 6803 is light dependent and yields a monocistronic mRNA, as determined by Northern analysis. Surprisingly, mutants with inactivated dnaA were viable. In batch cultures under standard conditions, the mutants grew like the wild type and di...

journal_title：Journal of bacteriology

authors： Richter S,Hagemann M,Messer W

更新日期：1998-09-01 00:00:00

abstract：:Lactate dehydrogenase (LDH) was studied in phage-propagating strains 29, 3A, 6, 81, and 42D of Staphylococcus aureus selected from the five groups in the International-Blair series. Cells were cultivated in Brain Heart Infusion (Difco) under nearly anaerobic conditions and were harvested near the end of the log phase....

journal_title：Journal of bacteriology

authors： Stockland AE,San Clemente CL

更新日期：1968-01-01 00:00:00

abstract：:Anabaena variabilis ATCC 29413 is a heterotrophic, nitrogen-fixing cyanobacterium that has been reported to fix nitrogen and reduce acetylene to ethane in the absence of molybdenum. DNA from this strain hybridized well at low stringency to the nitrogenase 2 (vnfDGK) genes of Azotobacter vinelandii. The hybridizing reg...

journal_title：Journal of bacteriology

authors： Thiel T

更新日期：1993-10-01 00:00:00

abstract：:Isotopic exchange of (35)S between penicillins and 6-amino-penicillanic acid (6-APA) was observed in cell-free extracts of Penicillium chrysogenum. Sulfhydryl-containing compounds were required for activity. Dithiothreitol, dithioerythritol, mercaptoethanol, and glutathione served as activators. The acyltransferase wa...

journal_title：Journal of bacteriology

authors： Pruess DL,Johnson MJ

更新日期：1967-11-01 00:00:00

abstract：:Escherichia coli K-12, polAl(-) is a mutant strain whose extracts are deficient in Kornberg deoxyribonucleic acid (DNA) polymerase activity. We have compared the mutant and parental strains on the basis of a number of responses to ultraviolet (UV) and X-irradiation. For both types of radiation, the mutant is more sens...

journal_title：Journal of bacteriology

authors： Paterson MC,Boyle JM,Setlow RB

更新日期：1971-07-01 00:00:00

abstract：:The allosteric pattern of control for 3-deoxy-d-arabino-heptulosonate 7-phosphate (DAHP) synthetase has previously been shown to be strongly conserved among the member species of a given genus in bacteria. The implications of this finding as a procedural tool of bacterial phylogeny were pursued by a study of two organ...

journal_title：Journal of bacteriology

authors： Jensen RA,Stenmark SL

更新日期：1970-03-01 00:00:00

abstract：:The tyrT (tRNA(1TYr)) locus of Escherichia coli B differs structurally from that of K-12 strains by the absence of 2 of 3.14 terminal repeat sequences. ...

journal_title：Journal of bacteriology

authors： Timms AR,Bridges BA

更新日期：1996-04-01 00:00:00

abstract：:Cell division of Escherichia coli K-12 strain PA3092 was inhibited by the addition of adenosine 3',5'-phosphate (cAMP), and the cellular morphology was changed from rods into filaments. Nucleoids in the filaments were regularly distributed and septum formation was perfectly inhibited. This inhibition of cell division ...

journal_title：Journal of bacteriology

authors： Utsumi R,Tanabe H,Nakamoto Y,Kawamukai M,Sakai H,Himeno M,Komano T,Hirota Y

更新日期：1981-09-01 00:00:00

abstract：:In this report we present the first description of the isolation and partial characterization of the deoxyribonucleic acid (DNA) polymerase activity from two species of Mycoplasmatales, Mycoplasma orale type 1 and M. hyorhinis. We have identified only a single DNA polymerase species in the mycoplasma crude extracts, a...

journal_title：Journal of bacteriology

authors： Mills LB,Stanbridge EJ,Sedwick WD,Korn D

更新日期：1977-11-01 00:00:00

abstract：:The competence-related phenotypes of mutations in each of the four open reading frames associated with the comE locus of Bacillus subtilis are described. comEA and comEC are required for transformability, whereas the products of comEB and of the overlapping comER, which is transcribed in the reverse direction, are dis...

journal_title：Journal of bacteriology

authors： Inamine GS,Dubnau D

更新日期：1995-06-01 00:00:00

abstract：:An IMP dehydrogenase gene was isolated from Candida albicans on a approximately 2.9-kb XbaI genomic DNA fragment. The putative Candida IMP dehydrogenase gene (IMH3) encodes a protein of 521 amino acids with extensive sequence similarity to the IMP dehydrogenases of Saccharomyces cerevisiae and various other organisms....

journal_title：Journal of bacteriology

authors： Köhler GA,White TC,Agabian N

更新日期：1997-04-01 00:00:00

abstract：:When 100 group A streptococcus isolates were screened, drs, a variant of sic, was identified in emm12 and emm55 isolates. Molecular characterization showed that the drs gene sequence is highly conserved, unlike the sic gene sequence. However, the variation in gene size observed was due to the presence of extra interna...

journal_title：Journal of bacteriology

authors： Sagar V,Kumar R,Ganguly NK,Menon T,Chakraborti A

更新日期：2007-04-01 00:00:00

abstract：:Mutants of Escherichia coli unable to synthesize a functional pyruvate formate-lyase (PFL) are severely impaired in their capacity to grow by glucose fermentation. In a functional complementation assay designed to isolate the pfl gene from Clostridium butyricum, we fortuitously identified a gene that did not encode a ...

journal_title：Journal of bacteriology

authors： Sawers G,Hesslinger C,Muller N,Kaiser M

更新日期：1998-07-01 00:00:00

abstract：:The genome of the Philadelphia-1 strain of Legionella pneumophila, the causative organism of Legionnaires' disease, encodes two virulence-associated type 4 secretion systems (T4SSs), the Dot/Icm type 4B (T4BSS) and the Lvh type 4A (T4ASS). Broth stationary-phase cultures of most dot/icm mutants are defective in entry ...

journal_title：Journal of bacteriology

authors： Bandyopadhyay P,Lang EA,Rasaputra KS,Steinman HM

更新日期：2013-08-01 00:00:00

abstract：:Sulfur-starved cells of Anacystis nidulans have an increased capacity to take up sulfate. The apparent Vmax for sulfate uptake increased at least 10-fold after 24 h of sulfur deprivation, whereas the K1/2 remained unchanged at approximately 1.35 microM. The initial rate of sulfate uptake increased between 2 and 6 h af...

journal_title：Journal of bacteriology

authors： Green LS,Grossman AR

更新日期：1988-02-01 00:00:00

abstract：:Methyl coenzyme M methylreductase from acetate-grown Methanosarcina thermophila TM-1 was purified 16-fold from a cell extract to apparent homogeneity as determined by native polyacrylamide gel electrophoresis. Ninety-four percent of the methylreductase activity was recovered in the soluble fraction of cell extracts. T...

journal_title：Journal of bacteriology

authors： Jablonski PE,Ferry JG

更新日期：1991-04-01 00:00:00

abstract：:D-Ribose isomerase was purified and crystallized from cells of Mycobacterium smegmatis grown on either D-ribose or L-rhamnose. Isomerase activity for both of these sugars remained together throughout the purification. The isomerase from L-rhamnose-grown cells had the same chemical and physical properties as the enzyme...

journal_title：Journal of bacteriology

authors： Izumori K,Mitchell M,Elbein AD

更新日期：1976-04-01 00:00:00