Deletion of the 389 N-terminal residues of the transcriptional activator AREA does not result in nitrogen metabolite derepression in Aspergillus nidulans.

Abstract:

:Utilizing a homologous gene replacement in order to retain the native promoter and 5' and 3' untranslated messenger regions (and thereby ensure physiological validity), we have shown that deletion of the N-terminal 389 amino acids of the transcriptional activator AREA does not result in nitrogen metabolite derepression in Aspergillus nidulans. Our results provide no evidence for a modulating interaction involving the N terminus of AREA and contrast with those of H. K. Lamb, A. L. Dodds, D. R. Swatman, E. Cairns, and A. R. Hawkins (J. Bacteriol. 179:6649-6656, 1997), who used nontargeted ectopic copies of a construct containing a heterologous promoter and untranslated regions. Results obtained with this deletion mutant, nevertheless, provide further evidence for the dispensability of large portions of AREA.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Caddick MX,Arst HN Jr

doi

10.1128/JB.180.21.5762-5764.1998

subject

Has Abstract

pub_date

1998-11-01 00:00:00

pages

5762-4

issue

21

eissn

0021-9193

issn

1098-5530

journal_volume

180

pub_type

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