Modulation of luminescence operon expression by N-octanoyl-L-homoserine lactone in ainS mutants of Vibrio fischeri.


:Population density-dependent expression of luminescence in Vibrio fischeri is controlled by the autoinducer N-3-oxohexanoyl-L-homoserine lactone (autoinducer 1 [AI-1]), which via LuxR activates transcription of the lux operon (luxICDABEG, encoding the putative autoinducer synthase [LuxI] and the luminescence enzymes). We recently identified a novel V. fischeri locus, ainS, necessary for the synthesis of a second autoinducer, N-octanoyl-L-homoserine lactone (AI-2), which via LuxR can activate lux operon transcription in the absence of AI-1. To define the regulatory role of AI-2, a luxI ainS double mutant was constructed; in contrast to the parental strain and a luxI mutant, the luxI ainS mutant exhibited no induction of luminescence and produced no detectable luminescence autoinducer, demonstrating that V. fischeri makes no luminescence autoinducers other than those whose synthesis is directed by luxI and ainS. A mutant defective only in ainS exhibited accelerated luminescence induction compared with that of the parental strain, indicating that AI-2 functions in V. fischeri to delay luminescence induction. Consistent with that observation, the exogenous addition of AI-2 inhibited induction in a dose-dependent manner in V. fischeri and Escherichia coli carrying the lux genes. AI-2 did not mediate luxR negative autoregulation, alone or in the presence of AI-1, and inhibited luminescence induction in E. coli regardless of whether luxR was under the control of its native promoter or a foreign one. Increasing amounts of AI-1 overcame the inhibitory effect of AI-2, and equal activation of luminescence required 25- to 45-fold-more AI-2 than AI-1. We conclude that AI-2 inhibits lux operon transcription. The data are consistent with a model in which AI-2 competitively inhibits the association of AI-1 with LuxR, forming a complex with LuxR which has a markedly lower lux operon-inducing specific activity than that of AI-1-LuxR. AI-2 apparently functions in V. fischeri to suppress or delay induction at low and intermediate population densities.


J Bacteriol


Journal of bacteriology


Kuo A,Callahan SM,Dunlap PV




Has Abstract


1996-02-01 00:00:00












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    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Ehrbar K,Hapfelmeier S,Stecher B,Hardt WD

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  • Two homologous genes coding for spore-specific proteins are expressed at different times during development of Myxococcus xanthus.

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    pub_type: 杂志文章


    authors: Teintze M,Thomas R,Furuichi T,Inouye M,Inouye S

    更新日期:1985-07-01 00:00:00

  • Circular and linear plasmids of Lyme disease spirochetes have extensive homology: characterization of a repeated DNA element.

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    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Zückert WR,Meyer J

    更新日期:1996-04-01 00:00:00

  • Biosynthesis of streptococcal cell walls: N-acetyl-D-muramic acid.

    abstract::Glucose-1-(14)C and acetylglucosamine-1-(14)C were added singly and together with equal amounts of the unlabeled reciprocal to Brain Heart Infusion and used for the culture of Streptococcus pyogenes. The labeling pattern of the rhamnose, glucosamine, and muramic acid in the cell wall supported an intermediary role for...

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    pub_type: 杂志文章


    authors: Barkulis SS,Boltralik JJ,Hankin H,Heymann H

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  • Possible utility of a fluorescent antibody technique in the serological identification of antagonistic Streptomyces.

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    journal_title:Journal of bacteriology

    pub_type: 杂志文章


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    更新日期:1962-01-01 00:00:00

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    journal_title:Journal of bacteriology

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  • Staphylococcal Muller phenomenon: relationship to the plasminogen-plasmin system.

    abstract::Quie, Paul G. (University of Minnesota, Minneapolis) and Lewis W. Wannamaker. Staphylococcal Muller phenomenon: relationship to the plasminogen-plasmin system. J. Bacteriol. 82:770-783. 1961.-The staphylococcal factor that produces particulate proteolysis (Muller phenomenon) in whole blood or hemoglobinserum agar plat...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章



    更新日期:1961-11-01 00:00:00

  • Uracil-DNA glycosylase of Thermoplasma acidophilum directs long-patch base excision repair, which is promoted by deoxynucleoside triphosphates and ATP/ADP, into short-patch repair.

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    journal_title:Journal of bacteriology

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    authors: Shimada A,Tamatukuri J,Ito E

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    pub_type: 杂志文章


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    pub_type: 杂志文章


    authors: Bleves S,Soscia C,Nogueira-Orlandi P,Lazdunski A,Filloux A

    更新日期:2005-06-01 00:00:00

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    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Leduc M,Frehel C,van Heijenoort J

    更新日期:1985-02-01 00:00:00

  • Occurrence of alpha-tocopherolquinone and alpha-tocopherolquinol in microorganisms.

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    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Hughes PE,Tove SB

    更新日期:1982-09-01 00:00:00

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    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Abraham SN,Land M,Ponniah S,Endres R,Hasty DL,Babu JP

    更新日期:1992-08-01 00:00:00