Analysis of DNA inversions in the shufflon of plasmid R64.

Abstract:

:The shufflon, a multiple DNA inversion system in the plasmid R64, consists of four DNA segments flanked and separated by seven 19-bp repeat sequences. Site-specific recombinations mediated by the rci product occur between each inverted repeat sequence, resulting in inversions of the four segments independently or in groups. The seven 19-bp repeat sequences are classified into four types (repeat-a, -b, -c, and -d), according to their 3-bp variable sequences. We individually cloned A, B, and C segments of the R64 shufflon and determined the in vivo inversion frequency of each segment. The inversion frequencies of three segments differed greatly. The inversion frequency declined in the following order: segments A, B, and C. Synthetic 19-mer oligonucleotides corresponding to both strands of repeat-a, -b, -c, and -d sequences were inserted into appropriate sites of pBR322. The rci-mediated DNA inversion occurred between two synthetic inverted repeats, indicating that the 19-bp inverted repeat sequences are the sole elements required in cis for the shufflon system. The inversion frequencies of DNA segments flanked by various sequences indicate that the four types of repeat sequences determine the inversion frequency of the four DNA segments of the R64 shufflon. Deletion of a DNA segment flanked by direct repeat sequences could not be detected.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Gyohda A,Funayama N,Komano T

doi

10.1128/jb.179.6.1867-1871.1997

subject

Has Abstract

pub_date

1997-03-01 00:00:00

pages

1867-71

issue

6

eissn

0021-9193

issn

1098-5530

journal_volume

179

pub_type

杂志文章
  • Coordination of Synthesis and Assembly of a Modular Membrane-Associated [NiFe]-Hydrogenase Is Determined by Cleavage of the C-Terminal Peptide.

    abstract:UNLABELLED:During biosynthesis of [NiFe]-hydrogenase 2 (Hyd-2) of Escherichia coli, a 15-amino-acid C-terminal peptide is cleaved from the catalytic large subunit precursor, pro-HybC. This peptide is removed only after NiFe(CN)2CO cofactor insertion by the Hyp accessory protein machinery has been completed, suggesting ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00437-15

    authors: Thomas C,Muhr E,Sawers RG

    更新日期:2015-09-01 00:00:00

  • Autolytic enzyme system of Streptococcus faecalis. IV. Electron microscopic observations of autolysin and lysozyme action.

    abstract::Cell walls (LOG walls) were isolated from cultures of Streptococcus faecalis ATCC 9790 in the exponential phase of growth. These walls were either allowed to undergo autolytic dissolution (in the presence or absence of trypsin) or wall autolysis was inactivated with sodium dodecylsulfate (SDS walls). Inactivated walls...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.96.5.1803-1810.1968

    authors: Shockman GD,Martin JT

    更新日期:1968-11-01 00:00:00

  • A bacitracin-resistant Bacillus subtilis gene encodes a homologue of the membrane-spanning subunit of the Bacillus licheniformis ABC transporter.

    abstract::Bacitracin is a peptide antibiotic nonribosomally produced by Bacillus licheniformis. The bcrABC genes which confer bacitracin resistance to the bacitracin producer encode ATP binding cassette (ABC) transporter proteins, which are hypothesized to pump out bacitracin from the cells. Bacillus subtilis 168, which has no ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.185.1.51-59.2003

    authors: Ohki R,Tateno K,Okada Y,Okajima H,Asai K,Sadaie Y,Murata M,Aiso T

    更新日期:2003-01-01 00:00:00

  • Cloning and expression of the exfoliative toxin B gene from Staphylococcus aureus.

    abstract::Exfoliative toxin type B is produced by bacteriophage group II strains of Staphylococcus aureus and is a causative agent of staphylococcal scalded-skin syndrome. In addition to exfoliative toxin B, most isolates also produce a bacteriocin and are immune to the action of the bacteriocin. These phenotypes, as well as re...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.166.2.574-580.1986

    authors: Jackson MP,Iandolo JJ

    更新日期:1986-05-01 00:00:00

  • Excision repair of thymine glycols, urea residues, and apurinic sites in Escherichia coli.

    abstract::The genetic requirements for the excision repair of thymine glycols, urea residues, and apurinic (AP) sites were examined by measuring the survival in Escherichia coli mutants of phi X174 replicative form (RF) I transfecting DNA containing selectively introduced lesions. phi X RF I DNA containing thymine glycols was i...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.170.8.3359-3366.1988

    authors: Laspia MF,Wallace SS

    更新日期:1988-08-01 00:00:00

  • Evidence for divalent cation (Ca2+)-stabilized oligomeric proteins and covalently bound protein-peptidoglycan complexes in the outer membrane of Rhizobium leguminosarum.

    abstract::Two unusual characteristics of some outer membrane proteins of Rhizobium leguminosarum are described. First, most of the major outer membrane proteins could only be visualized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis after lysozyme treatment of the isolated cell envelopes, suggesting a very strong,...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.7.3989-3995.1989

    authors: de Maagd RA,Wientjes FB,Lugtenberg BJ

    更新日期:1989-07-01 00:00:00

  • Genomic comparison of Mycobacterium avium subsp. paratuberculosis sheep and cattle strains by microarray hybridization.

    abstract::Microarray-based comparisons of three Mycobacterium avium subsp. paratuberculosis isolates, including one sheep strain and two cattle strains, identified three large genomic deletions in the sheep strain, totaling 29,208 bp and involving 24 open reading frames. These deletions may help explain some of the differences ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.188.6.2290-2293.2006

    authors: Marsh IB,Bannantine JP,Paustian ML,Tizard ML,Kapur V,Whittington RJ

    更新日期:2006-03-01 00:00:00

  • Production of nitric oxide and nitrous oxide during denitrification by Corynebacterium nephridii.

    abstract::Resting cells of Corynebacterium nephridii reduce nitrate, nitrite, and nitric oxide to nitrous oxide under anaerobic conditions. Nitrous oxide production from nitrite was optimal from pH 7.0 to 7.4. The stoichiometry of nitrous oxide production from nitrite was 99% of the theoretical-two moles of nitrite was used for...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.101.3.821-826.1970

    authors: Renner ED,Becker GE

    更新日期:1970-03-01 00:00:00

  • Reversibility of SOS-associated division inhibition in Escherichia coli.

    abstract::In Escherichia coli the SOS response, induced by DNA-damaging treatments, includes two systems of cell division inhibition, SfiA and SfiC, which are thought to prevent cell division by interacting with the division protein FtsZ. It is shown here that SfiA-mediated division inhibition is readily reversible, even in the...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.166.3.733-738.1986

    authors: Maguin E,Lutkenhaus J,D'Ari R

    更新日期:1986-06-01 00:00:00

  • Insertional inactivation of genes encoding components of the sodium-type flagellar motor and switch of Vibrio parahaemolyticus.

    abstract::Vibrio parahaemolyticus possesses two types of flagella, polar and lateral, powered by distinct energy sources, which are derived from the sodium and proton motive forces, respectively. Although proton-powered flagella in Escherichia coli and Salmonella enterica serovar Typhimurium have been extensively studied, the m...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.182.4.1035-1045.2000

    authors: Boles BR,McCarter LL

    更新日期:2000-02-01 00:00:00

  • Growth phase variation in cell and nucleoid morphology in a Bacillus subtilis recA mutant.

    abstract::The major role of RecA is thought to be in helping repair and restart stalled replication forks. During exponential growth, Bacillus subtilis recA cells exhibited few microscopically observable nucleoid defects. However, the efficiency of plating was about 12% of that of the parent strain. A substantial and additive d...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.9.2963-2968.2001

    authors: Sciochetti SA,Blakely GW,Piggot PJ

    更新日期:2001-05-01 00:00:00

  • Purification and properties of methyl coenzyme M methylreductase from acetate-grown Methanosarcina thermophila.

    abstract::Methyl coenzyme M methylreductase from acetate-grown Methanosarcina thermophila TM-1 was purified 16-fold from a cell extract to apparent homogeneity as determined by native polyacrylamide gel electrophoresis. Ninety-four percent of the methylreductase activity was recovered in the soluble fraction of cell extracts. T...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.8.2481-2487.1991

    authors: Jablonski PE,Ferry JG

    更新日期:1991-04-01 00:00:00

  • Characterization of a pseudomonad 2-nitrobenzoate nitroreductase and its catabolic pathway-associated 2-hydroxylaminobenzoate mutase and a chemoreceptor involved in 2-nitrobenzoate chemotaxis.

    abstract::Pseudomonas fluorescens strain KU-7 is a prototype microorganism that metabolizes 2-nitrobenzoate (2-NBA) via the formation of 3-hydroxyanthranilate (3-HAA), a known antioxidant and reductant. The initial two steps leading to the sequential formation of 2-hydroxy/aminobenzoate and 3-HAA are catalyzed by a NADPH-depend...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01098-06

    authors: Iwaki H,Muraki T,Ishihara S,Hasegawa Y,Rankin KN,Sulea T,Boyd J,Lau PC

    更新日期:2007-05-01 00:00:00

  • Mating pheromones of Saccharomyces kluyveri: pheromone interactions between Saccharomyces kluyveri and Saccharomyces cerevisiae.

    abstract::Saccharomyces kluyveri is a heterothallic yeast with two allelic mating types denoted as a-k and alpha-k by analogy with Saccharomyces cerevisiae and from the work described here. S. kluyveri produces mating pheromones analogous to those of S. cerevisiae, but which appear to have different specificity. S. kluyveri thu...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.138.1.146-154.1979

    authors: McCullough J,Herskowitz I

    更新日期:1979-04-01 00:00:00

  • Transcriptional regulation of the Staphylococcus aureus thioredoxin and thioredoxin reductase genes in response to oxygen and disulfide stress.

    abstract::In this report we describe the cloning, organization, and promoter analysis of the Staphylococcus aureus thioredoxin (trxA) and thioredoxin reductase (trxB) genes and their transcription in response to changes in oxygen concentration and to oxidative stress compounds. Northern analysis showed that the S. aureus trxA a...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.186.2.326-334.2004

    authors: Uziel O,Borovok I,Schreiber R,Cohen G,Aharonowitz Y

    更新日期:2004-01-01 00:00:00

  • CHANGES IN MOUSE PNEUMONITIS AGENT ASSOCIATED WITH DEVELOPMENT OF RESISTANCE TO CHLORTETRACYCLINE.

    abstract::Moulder, James W. (University of Chicago, Chicago, Ill.), Dorothy L. Novosel, and Ilse I. E. Tribby. Changes in mouse pneumonitis agent associated with development of resistance to chlortetracycline. J. Bacteriol. 89:17-22. 1965.-A chlortetracycline-resistant mutant of mouse pneumonitis agent, a member of the psittaco...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.89.1.17-22.1965

    authors: MOULDER JW,NOVOSEL DL,TRIBBY II

    更新日期:1965-01-01 00:00:00

  • Influence of methionine pool composition on the formation of methyl-deficient transfer ribonucleic acid in Saccharomyces cerevisiae.

    abstract::Methionine auxotrophs of Saccharomyces cerevisiae continue to synthesize ribonucleic acid (RNA) after methionine withdrawal. The newly synthesized transfer RNA (tRNA) is methyl-deficient in some strains, but not in all. Whether such tRNA will accumulate depends on the position of the block in the methionine pathway th...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.100.2.687-694.1969

    authors: Kjellin-Stråby K

    更新日期:1969-11-01 00:00:00

  • Yaba tumor poxvirus synthesis in vitro. I. Cytopathological, histochemical, and immunofluorescent studies.

    abstract::Yohn, David S. (Roswell Park Memorial Institute, Buffalo, N.Y.), Victoria A. Haendiges, and James T. Grace, Jr. Yaba tumor poxvirus synthesis in vitro. I. Cytopathological, histochemical, and immunofluorescent studies. J. Bacteriol. 91:1977-1985. 1966.-Yaba tumor poxvirus synthesis in BSC-1 cell culture was followed s...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.91.5.1977-1985.1966

    authors: Yohn DS,Haendiges VA,Grace JT Jr

    更新日期:1966-05-01 00:00:00

  • Transformation and transfection of Pseudomonas aeruginosa: effects of metal ions.

    abstract::The ability of different metal ions to promote transformation of Pseudomonas aeruginosa by deoxyribonucleic acid of the plasmid RP1 was examined. CaCl2, MgCl2, and MnCl2 were found to promote such transformation, although at different frequencies and with the optimum response at different concentrations. Only MgCl2 pr...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.140.1.37-42.1979

    authors: Mercer AA,Loutit JS

    更新日期:1979-10-01 00:00:00

  • Mating types and macrocyst formation in Dictyostelium.

    abstract::Macrocysts have been found among strains of Dictyostelium rosarium Raper and Cavender (K. B. Raper and J. C. Cavender, J. Elisha Mitchell Sci. Soc. 84:31-47, 1968). Strains that exhibit mating capabilities could be classified into one of three mating types: A1, A2, or A3. Each mating type was self-incompatible but cro...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.147.3.1049-1053.1981

    authors: Chang MT,Raper KB

    更新日期:1981-09-01 00:00:00

  • The leucine-responsive regulatory protein, Lrp, activates transcription of the fim operon in Salmonella enterica serovar typhimurium via the fimZ regulatory gene.

    abstract::The fim operon of Salmonella enterica serovar Typhimurium encodes type 1 fimbriae. The expression of fim is controlled in response to environmental signals through a complex regulatory cascade involving the proteins FimW, FimY, and FimZ and a genetic locus, fimU, that encodes a rare arginine tRNA. We discovered that a...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01388-07

    authors: McFarland KA,Lucchini S,Hinton JC,Dorman CJ

    更新日期:2008-01-01 00:00:00

  • Relation of catalase to substrate utilization by Mycoplasma pneumoniae.

    abstract::No catalase activity was detected in four strains of glucose-grown Mycoplasma pneumoniae at any time during the replication of the organism. Exogenous catalase dramatically increased the O(2) uptake with glycerol, presumably by releasing inhibition caused by hydrogen peroxide. The effect of added catalase on the O(2) ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.95.4.1425-1430.1968

    authors: Low IE,Eaton MD,Proctor P

    更新日期:1968-04-01 00:00:00

  • Genetic analysis of the tdcABC operon of Escherichia coli K-12.

    abstract::The biodegradative threonine dehydratase (tdc) operon was mapped at 68 min on the Escherichia coli K-12 chromosome. The order of markers in the clockwise direction was dnaG uxaA tdc argG. A tdc deletion was isolated and mapped to this region of the chromosome. By using a tdcB-lacZ fusion the clockwise direction of tra...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.170.11.5360-5363.1988

    authors: Schweizer HP,Datta P

    更新日期:1988-11-01 00:00:00

  • Isolation and preliminary characterization of Saccharomyces cerevisiae proline auxotrophs.

    abstract::Proline-requiring mutants of Saccharomyces cerevisiae were isolated. Each mutation is recessive and is inherited as expected for a single nuclear gene. Three complementation groups cold be defined which are believed to correspond to mutations in the three genes (pro1, pro2, and pro3) coding for the three enzymes of th...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.138.3.816-822.1979

    authors: Brandriss MC

    更新日期:1979-06-01 00:00:00

  • FpvIR control of fpvA ferric pyoverdine receptor gene expression in Pseudomonas aeruginosa: demonstration of an interaction between FpvI and FpvR and identification of mutations in each compromising this interaction.

    abstract::FpvR is a presumed cytoplasmic membrane-associated anti-sigma factor that controls the activities of extracytoplasmic function sigma factors PvdS and FpvI responsible for transcription of pyoverdine biosynthetic genes and the ferric pyoverdine receptor gene, fpvA, respectively. Using deletion analysis and an in vivo b...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.187.16.5648-5657.2005

    authors: Rédly GA,Poole K

    更新日期:2005-08-01 00:00:00

  • Characterization of a dominant, constitutive mutation, PHOO, for the repressible acid phosphatase synthesis in Saccharomyces cerevisiae.

    abstract::An apparent operator-constitutive mutation was discovered in the repressible acid phosphatase system in Saccharomyces cerevisiae. The site of mutation, designated PHOO, was found to be closely linked to the phoD locus. The mutant allele, PHOO, was semidominant over the wild-type allele and effective for the expression...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.120.2.608-617.1974

    authors: Toh-E A,Oshima Y

    更新日期:1974-11-01 00:00:00

  • Stabilization of the pertussis toxin secretion apparatus by the C terminus of PtlD.

    abstract::Pertussis toxin (PT) is secreted from Bordetella pertussis by a type IV secretion system, known as the Ptl transporter, that comprises nine different proteins, PtlA to PtlI. In this study, we found that PtlD is required for the stability of three Ptl proteins, PtlE, PtlF, and PtlH. A region limited to the C-terminal 7...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01106-08

    authors: Verma A,Cheung AM,Burns DL

    更新日期:2008-11-01 00:00:00

  • Isolation and preliminary characterization of auxotrophs of a filamentous Cyanobacterium.

    abstract::Auxotrophic mutants of the filamentous cyanobacterium Anabaena variabilis were isolated by a method in which, after mutagenesis and before penicllin enrichment, mutant and wild-type cells were separated by cavitation. Auxotrophs were identified by their inability to grow on minimal medium, and they were partially char...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.129.3.1556-1562.1977

    authors: Currier TC,Haury JF,Wolk CP

    更新日期:1977-03-01 00:00:00

  • Localization of c-di-GMP-binding protein with the linear terminal complexes of Acetobacter xylinum.

    abstract::Specific labeling of a single row of cellulose-synthesizing complexes (terminal complexes, TC subunits, TCs, or TC arrays) in Acetobacter xylinum by antibodies raised against a 93-kDa protein (the cyclic dignanylic acid-binding protein) has been demonstrated by using the sodium dodecyl sulfate (SDS)-freeze-fracture la...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.19.5668-5674.2001

    authors: Kimura S,Chen HP,Saxena IM,Brown RM Jr,Itoh T

    更新日期:2001-10-01 00:00:00

  • Peptide wrwycr inhibits the excision of several prophages and traps holliday junctions inside bacteria.

    abstract::Peptide inhibitors of phage lambda site-specific recombination were previously isolated by screening synthetic combinatorial peptide libraries. These inhibitors cause the accumulation of complexes between the recombinase and the Holliday junction intermediate of several highly divergent tyrosine recombinases. Peptide ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01559-08

    authors: Gunderson CW,Boldt JL,Authement RN,Segall AM

    更新日期:2009-04-01 00:00:00