Abstract:
:The shufflon, a multiple DNA inversion system in the plasmid R64, consists of four DNA segments flanked and separated by seven 19-bp repeat sequences. Site-specific recombinations mediated by the rci product occur between each inverted repeat sequence, resulting in inversions of the four segments independently or in groups. The seven 19-bp repeat sequences are classified into four types (repeat-a, -b, -c, and -d), according to their 3-bp variable sequences. We individually cloned A, B, and C segments of the R64 shufflon and determined the in vivo inversion frequency of each segment. The inversion frequencies of three segments differed greatly. The inversion frequency declined in the following order: segments A, B, and C. Synthetic 19-mer oligonucleotides corresponding to both strands of repeat-a, -b, -c, and -d sequences were inserted into appropriate sites of pBR322. The rci-mediated DNA inversion occurred between two synthetic inverted repeats, indicating that the 19-bp inverted repeat sequences are the sole elements required in cis for the shufflon system. The inversion frequencies of DNA segments flanked by various sequences indicate that the four types of repeat sequences determine the inversion frequency of the four DNA segments of the R64 shufflon. Deletion of a DNA segment flanked by direct repeat sequences could not be detected.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Gyohda A,Funayama N,Komano Tdoi
10.1128/jb.179.6.1867-1871.1997subject
Has Abstractpub_date
1997-03-01 00:00:00pages
1867-71issue
6eissn
0021-9193issn
1098-5530journal_volume
179pub_type
杂志文章abstract:UNLABELLED:During biosynthesis of [NiFe]-hydrogenase 2 (Hyd-2) of Escherichia coli, a 15-amino-acid C-terminal peptide is cleaved from the catalytic large subunit precursor, pro-HybC. This peptide is removed only after NiFe(CN)2CO cofactor insertion by the Hyp accessory protein machinery has been completed, suggesting ...
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journal_title:Journal of bacteriology
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1970-03-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.166.3.733-738.1986
更新日期:1986-06-01 00:00:00
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pub_type: 杂志文章
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2001-05-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.8.2481-2487.1991
更新日期:1991-04-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01098-06
更新日期:2007-05-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.138.1.146-154.1979
更新日期:1979-04-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.186.2.326-334.2004
更新日期:2004-01-01 00:00:00
abstract::Moulder, James W. (University of Chicago, Chicago, Ill.), Dorothy L. Novosel, and Ilse I. E. Tribby. Changes in mouse pneumonitis agent associated with development of resistance to chlortetracycline. J. Bacteriol. 89:17-22. 1965.-A chlortetracycline-resistant mutant of mouse pneumonitis agent, a member of the psittaco...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.89.1.17-22.1965
更新日期:1965-01-01 00:00:00
abstract::Methionine auxotrophs of Saccharomyces cerevisiae continue to synthesize ribonucleic acid (RNA) after methionine withdrawal. The newly synthesized transfer RNA (tRNA) is methyl-deficient in some strains, but not in all. Whether such tRNA will accumulate depends on the position of the block in the methionine pathway th...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.100.2.687-694.1969
更新日期:1969-11-01 00:00:00
abstract::Yohn, David S. (Roswell Park Memorial Institute, Buffalo, N.Y.), Victoria A. Haendiges, and James T. Grace, Jr. Yaba tumor poxvirus synthesis in vitro. I. Cytopathological, histochemical, and immunofluorescent studies. J. Bacteriol. 91:1977-1985. 1966.-Yaba tumor poxvirus synthesis in BSC-1 cell culture was followed s...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.91.5.1977-1985.1966
更新日期:1966-05-01 00:00:00
abstract::The ability of different metal ions to promote transformation of Pseudomonas aeruginosa by deoxyribonucleic acid of the plasmid RP1 was examined. CaCl2, MgCl2, and MnCl2 were found to promote such transformation, although at different frequencies and with the optimum response at different concentrations. Only MgCl2 pr...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.140.1.37-42.1979
更新日期:1979-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.147.3.1049-1053.1981
更新日期:1981-09-01 00:00:00
abstract::The fim operon of Salmonella enterica serovar Typhimurium encodes type 1 fimbriae. The expression of fim is controlled in response to environmental signals through a complex regulatory cascade involving the proteins FimW, FimY, and FimZ and a genetic locus, fimU, that encodes a rare arginine tRNA. We discovered that a...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01388-07
更新日期:2008-01-01 00:00:00
abstract::No catalase activity was detected in four strains of glucose-grown Mycoplasma pneumoniae at any time during the replication of the organism. Exogenous catalase dramatically increased the O(2) uptake with glycerol, presumably by releasing inhibition caused by hydrogen peroxide. The effect of added catalase on the O(2) ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.95.4.1425-1430.1968
更新日期:1968-04-01 00:00:00
abstract::The biodegradative threonine dehydratase (tdc) operon was mapped at 68 min on the Escherichia coli K-12 chromosome. The order of markers in the clockwise direction was dnaG uxaA tdc argG. A tdc deletion was isolated and mapped to this region of the chromosome. By using a tdcB-lacZ fusion the clockwise direction of tra...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.11.5360-5363.1988
更新日期:1988-11-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.138.3.816-822.1979
更新日期:1979-06-01 00:00:00
abstract::FpvR is a presumed cytoplasmic membrane-associated anti-sigma factor that controls the activities of extracytoplasmic function sigma factors PvdS and FpvI responsible for transcription of pyoverdine biosynthetic genes and the ferric pyoverdine receptor gene, fpvA, respectively. Using deletion analysis and an in vivo b...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.16.5648-5657.2005
更新日期:2005-08-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.120.2.608-617.1974
更新日期:1974-11-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2008-11-01 00:00:00
abstract::Auxotrophic mutants of the filamentous cyanobacterium Anabaena variabilis were isolated by a method in which, after mutagenesis and before penicllin enrichment, mutant and wild-type cells were separated by cavitation. Auxotrophs were identified by their inability to grow on minimal medium, and they were partially char...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.129.3.1556-1562.1977
更新日期:1977-03-01 00:00:00
abstract::Specific labeling of a single row of cellulose-synthesizing complexes (terminal complexes, TC subunits, TCs, or TC arrays) in Acetobacter xylinum by antibodies raised against a 93-kDa protein (the cyclic dignanylic acid-binding protein) has been demonstrated by using the sodium dodecyl sulfate (SDS)-freeze-fracture la...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.19.5668-5674.2001
更新日期:2001-10-01 00:00:00
abstract::Peptide inhibitors of phage lambda site-specific recombination were previously isolated by screening synthetic combinatorial peptide libraries. These inhibitors cause the accumulation of complexes between the recombinase and the Holliday junction intermediate of several highly divergent tyrosine recombinases. Peptide ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01559-08
更新日期:2009-04-01 00:00:00