Genomic comparison of Mycobacterium avium subsp. paratuberculosis sheep and cattle strains by microarray hybridization.

abstract：:We have isolated a hybrid plasmid, pDB(mei2)2, containing a 7.4-kilobases (kb) DNA fragment from a Schizosaccharomyces pombe genomic library which is able to complement the mei2 mutation of S. pombe. Integration of the cloned DNA sequence at the mei2 site on chromosome I demonstrated that it contained the mei2 gene. T...

journal_title：Journal of bacteriology

authors： Shimoda C,Uehira M,Kishida M,Fujioka H,Iino Y,Watanabe Y,Yamamoto M

更新日期：1987-01-01 00:00:00

abstract：:The competence-related phenotypes of mutations in each of the four open reading frames associated with the comE locus of Bacillus subtilis are described. comEA and comEC are required for transformability, whereas the products of comEB and of the overlapping comER, which is transcribed in the reverse direction, are dis...

journal_title：Journal of bacteriology

authors： Inamine GS,Dubnau D

更新日期：1995-06-01 00:00:00

abstract：:A 200-megadalton plasmid was mobilized from Rhizobium japonicum USDA 191 to other Rhizobium strains either that cannot nodulate soybeans or that form Fix- nodules on certain cultivars. The symbiotic properties of the transconjugants indicate that both soybean specificity for nodulation and cultivar specificity for nit...

journal_title：Journal of bacteriology

authors： Appelbaum ER,McLoughlin TJ,O'Connell M,Chartrain N

更新日期：1985-07-01 00:00:00

abstract：:The imp genes, responsible for the UV protection and mutation effects of the I incompatibility group plasmid TP110, have been cloned into vector plasmids, and their products have been analyzed. The genetic information required for expression of these properties was carried in a continuous DNA sequence of approximately...

journal_title：Journal of bacteriology

authors： Glazebrook JA,Grewal KK,Strike P

更新日期：1986-10-01 00:00:00

abstract：:The amounts of glycogen and trehalose have been measured in cells of a prototrophic diploid yeast strain subjected to a variety of nutrient limitations. Both glycogen and trehalose were accumulated in cells deprived specifically of nirogen, sulfur, or phosphorus, suggesting that reserve carbohydrate accumulation is a ...

journal_title：Journal of bacteriology

authors： Lillie SH,Pringle JR

更新日期：1980-09-01 00:00:00

abstract：:DNA sequence analysis revealed that the putative yhdJ DNA methyltransferase gene of Escherichia coli is 55% identical to the Nostoc sp. strain PCC7120 gene encoding DNA methyltransferase AvaIII, which methylates adenine in the recognition sequence, ATGCAT. The yhdJ gene was cloned, and the enzyme was overexpressed and...

journal_title：Journal of bacteriology

authors： Kossykh VG,Lloyd RS

更新日期：2004-04-01 00:00:00

abstract：:Members of all four families of methanogenic bacteria were analyzed for polyamine concentrations. High-performance liquid chromatography analysis of dansylated cell extracts revealed typical polyamine patterns for each family. Members of Methanobacteriaceae (family I) were characterized by very low polyamine concentra...

journal_title：Journal of bacteriology

authors： Scherer P,Kneifel H

更新日期：1983-06-01 00:00:00

abstract：:Gundersen, Wenche B. (Oslo University, Oslo, Norway). New type of streptomycin resistance resulting from action of the episomelike mutator factor in Escherichia coli. J. Bacteriol. 86:510-516. 1963.-Analyses have been performed to elucidate the genetic nature of the streptomycin resistance that results from the action...

journal_title：Journal of bacteriology

authors： GUNDERSEN WB

更新日期：1963-09-01 00:00:00

abstract：:The relationship between specific growth rate of Escherichia coli and the concentration of limiting nutrient (glucose or phosphate or tryptophan) has been determined for populations in a steady state. At high concentrations the specific growth rate is independent of the concentration of nutrient, but at low concentrat...

journal_title：Journal of bacteriology

authors： Shehata TE,Marr AG

更新日期：1971-07-01 00:00:00

abstract：:The PRO1 gene of Saccharomyces cerevisiae encodes the 428-amino-acid protein gamma-glutamyl kinase (ATP:L-glutamate 5-phosphotransferase, EC 2.7.2.11), which catalyzes the first step in proline biosynthesis. Amino acid sequence comparison revealed significant homology between the yeast and Escherichia coli gamma-gluta...

journal_title：Journal of bacteriology

authors： Li W,Brandriss MC

更新日期：1992-06-01 00:00:00

abstract：:The deacetylcephalosporin C synthetase (hydroxylase) gene from Streptomyces clavuligerus has been cloned and sequenced. The open reading frame codes for a protein with an Mr of 34,584. The hydroxylase gene (cefF) is closely linked to the epimerase gene (cefD) and the expandase gene (cefE) and is transcribed in the opp...

journal_title：Journal of bacteriology

authors： Kovacevic S,Miller JR

更新日期：1991-01-01 00:00:00

abstract：:Phosphoenolpyruvate carboxylase (EC 4.1.1.31) from Azotobacter vinelandii, like the corresponding enzyme from other organisms, is activated by acetyl coenzyme A and inhibited by l-aspartate. Both modifiers affect primarily the affinity of the enzyme for phosphoenolpyruvate. This is the first enzyme with a strictly ana...

journal_title：Journal of bacteriology

authors： Liao CL,Atkinson DE

更新日期：1971-04-01 00:00:00

abstract：:The distribution of bacterial L-ornithine: alpha-ketoglutarate delta-aminotransferase (L-ornithine:2-oxo-acid aminotransferase [EC 2.6.1.13]) was investigated, and Bacillus sphaericus (IFO 3525) was found to have the highest activity of the enzyme, which was inducibly formed by addition of L-ornithine or L-arginine to...

journal_title：Journal of bacteriology

authors： Yasuda M,Tanizawa K,Misono H,Toyama S,Soda K

更新日期：1981-10-01 00:00:00

abstract：:Many of the gene products that participate in nitrogen metabolism are sensitive to nitrogen catabolite repression (NCR), i.e., their expression is decreased to low levels when readily used nitrogen sources such as asparagine are provided. Previous work has shown this NCR sensitivity requires the cis-acting UASNTR elem...

journal_title：Journal of bacteriology

authors： Coffman JA,el Berry HM,Cooper TG

更新日期：1994-12-01 00:00:00

abstract：:The distribution of a pulse of teichoic acid-specific radiolabel between wall and membrane teichoic acids in pneumococci was constant over a subsequent chase period, suggesting that wall and membrane teichoic acids are biosynthesized simultaneously and independently. ...

journal_title：Journal of bacteriology

authors： Briles EB,Tomasz A

更新日期：1975-04-01 00:00:00

abstract：:The repressive effects of exogenous cytidine on growing cells was examined in a specially constructed strain in which the pool sizes of endogenous uridine 5'-diphosphate and uridine 5'-triphosphate cannot be varied by the addition of uracil and/or uridine to the medium. Five enzymes of the pyrimidine biosynthetic path...

journal_title：Journal of bacteriology

authors： Kelln RA,Kinahan JJ,Foltermann KF,O'Donovan GA

更新日期：1975-11-01 00:00:00

abstract：:Although nitrate stimulated the capacity of Clostridium thermoautotrophicum and Clostridium thermoaceticum to oxidize (utilize) substrates under heterotrophic conditions, it inhibited autotrophic H2-CO2-dependent growth. Under basal medium conditions, nitrate was also inhibitory to the use of one-carbon substrates (i....

journal_title：Journal of bacteriology

authors： Fröstl JM,Seifritz C,Drake HL

更新日期：1996-08-01 00:00:00

abstract：:We have cloned a lytic enzyme, PlyPH, with a specific lytic effect on Bacillus anthracis strains. PlyPH remains active between pH 4 and 10.5, and a single dose rescued a significant percentage of mice infected intraperitoneally with an attenuated B. anthracis strain. We propose PlyPH as a novel therapeutic agent. ...

journal_title：Journal of bacteriology

authors： Yoong P,Schuch R,Nelson D,Fischetti VA

更新日期：2006-04-01 00:00:00

abstract：:S1 nuclease mapping and Northern (RNA) hybridization revealed that the rpoDA gene encoding the principal sigma subunit of Pseudomonas aeruginosa PAO1 is transcribed as a monocistronic mRNA of 2 kb and that the transcription from the rpoDA promoter (PC) starts 32 bases upstream from the first nucleotide of the initiati...

journal_title：Journal of bacteriology

authors： Fujita M,Tanaka K,Takahashi H,Amemura A

更新日期：1993-02-01 00:00:00

abstract：:The bacterial response to stress is controlled by two proteins, RelA and SpoT. RelA generates the alarmone (p)ppGpp under amino acid starvation, whereas SpoT is responsible for (p)ppGpp hydrolysis and for synthesis of (p)ppGpp under a variety of cellular stress conditions. It is widely accepted that RelA is associated...

journal_title：Journal of bacteriology

authors： Jiang M,Sullivan SM,Wout PK,Maddock JR

更新日期：2007-09-01 00:00:00

abstract：:Aerosols of Mycoplasma pneumoniae were studied at several relative humidities at a controlled temperature of 27 C. Production of an experimentally reproducible aerosol required preatomization of the organism in its suspending fluid and was dependent on the type of fluid used in atomization as well as on the procedures...

journal_title：Journal of bacteriology

authors： Wright DN,Bailey GD,Hatch MT

更新日期：1968-10-01 00:00:00

abstract：:A DNA fragment from Xanthomonas campestris pv. campestris that partially restored the carbohydrate fermentation pattern of a cya crp Escherichia coli strain was cloned and expressed in E. coli. The nucleotide sequence of this fragment revealed the presence of a 700-base-pair open reading frame that coded for a protein...

journal_title：Journal of bacteriology

authors： de Crecy-Lagard V,Glaser P,Lejeune P,Sismeiro O,Barber CE,Daniels MJ,Danchin A

更新日期：1990-10-01 00:00:00

abstract：:The brucellae are the etiological agents of brucellosis, a worldwide-distributed zoonosis. These bacteria are facultative intracellular parasites and thus are able to adjust their metabolism to the extra- and intracellular environments encountered during an infectious cycle. However, this aspect of Brucella biology is...

journal_title：Journal of bacteriology

authors： Zúñiga-Ripa A,Barbier T,Conde-Álvarez R,Martínez-Gómez E,Palacios-Chaves L,Gil-Ramírez Y,Grilló MJ,Letesson JJ,Iriarte M,Moriyón I

更新日期：2014-08-15 00:00:00

abstract：:The AOX1 gene, which encodes an alternative oxidase, was isolated from the genomic DNA library of Candida albicans. The gene encodes a polypeptide consisting of 379 amino acids with a calculated molecular mass of 43,975 Da. The aox1/aox1 mutant strain did not show cyanide-resistant respiration under normal conditions ...

journal_title：Journal of bacteriology

authors： Huh WK,Kang SO

更新日期：1999-07-01 00:00:00

abstract：:By extracellular labeling of peptides of intact Corynebacterium diphtheriae, followed by fractionation of the cells and chain completion by isolated polysomes, it is shown that diphtheria toxin is formed and secreted cotranslationally by membrane-bound polysomes; free polysomes from none. Moreover, when the chains on ...

journal_title：Journal of bacteriology

authors： Smith WP,Tai PC,Murphy JR,Davis BD

更新日期：1980-01-01 00:00:00

abstract：:We determined the nucleotide sequence of a 1.9-kilobase fragment of Pseudomonas paucimobilis SYK6 chromosomal DNA that included genes encoding protocatechuate 4,5-dioxygenase, the enzyme responsible for the aromatic ring fission of protocatechuate. Two open reading frames of 417 and 906 base pairs were found that had ...

journal_title：Journal of bacteriology

authors： Noda Y,Nishikawa S,Shiozuka K,Kadokura H,Nakajima H,Yoda K,Katayama Y,Morohoshi N,Haraguchi T,Yamasaki M

更新日期：1990-05-01 00:00:00

abstract：:Gel retardation experiments indicated the presence in Pseudomonas aeruginosa cell extracts of an arginine-inducible DNA-binding protein that interacts with the control regions for the car and argF operons, encoding carbamoylphosphate synthetase and anabolic ornithine carbamoyltransferase, respectively. Both enzymes ar...

journal_title：Journal of bacteriology

authors： Park SM,Lu CD,Abdelal AT

更新日期：1997-09-01 00:00:00

abstract：:The complete Bacillus subtilis genome contains two genes with the potential to encode glutamate dehydrogenase (GlutDH) enzymes. Mutations in these genes were constructed and characterized. The rocG gene proved to encode a major GlutDH whose synthesis was induced in media containing arginine or ornithine or, to a lesse...

journal_title：Journal of bacteriology

authors： Belitsky BR,Sonenshein AL

更新日期：1998-12-01 00:00:00

abstract：:Streptomyces clavuligerus produces at least five different clavam metabolites, including clavulanic acid and the methionine antimetabolite, alanylclavam. In vitro transposon mutagenesis was used to analyze a 13-kb region upstream of the known paralogue gene cluster. The paralogue cluster includes one group of clavulan...

journal_title：Journal of bacteriology

authors： Zelyas NJ,Cai H,Kwong T,Jensen SE

更新日期：2008-12-01 00:00:00

abstract：:AraC protein, a transcriptional regulator of the L-arabinose operon in Escherichia coli, is dimeric. Each monomer consists of a domain for DNA binding plus transcription activation and a domain for dimerization plus arabinose binding. These are connected to one another by a linker region of at least 5 amino acids. Her...

journal_title：Journal of bacteriology

authors： Eustance RJ,Schleif RF

更新日期：1996-12-01 00:00:00