Abstract:
:We have cloned a lytic enzyme, PlyPH, with a specific lytic effect on Bacillus anthracis strains. PlyPH remains active between pH 4 and 10.5, and a single dose rescued a significant percentage of mice infected intraperitoneally with an attenuated B. anthracis strain. We propose PlyPH as a novel therapeutic agent.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Yoong P,Schuch R,Nelson D,Fischetti VAdoi
10.1128/JB.188.7.2711-2714.2006keywords:
subject
Has Abstractpub_date
2006-04-01 00:00:00pages
2711-4issue
7eissn
0021-9193issn
1098-5530pii
188/7/2711journal_volume
188pub_type
杂志文章abstract::The terminal oxidase content of Bacillus firmus OF4, a facultative alkaliphile that grows well over the pH range of 7.5 to 10.5, was studied by difference spectroscopy. Evidence was found for three terminal oxidases under different growth conditions. The growth pH and the stage of growth profoundly affected the expres...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.16.5010-5016.1991
更新日期:1991-08-01 00:00:00
abstract::Sortases are transamidases that covalently link proteins to the peptidoglycan of gram-positive bacteria. The genome of the pathogenic bacterium Listeria monocytogenes encodes two sortases genes, srtA and srtB. The srtA gene product anchors internalin and some other LPXTG-containing proteins to the listerial surface. H...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.186.7.1972-1982.2004
更新日期:2004-04-01 00:00:00
abstract::The CpxA-CpxR two-component signal transduction pathway of Escherichia coli was studied in a mutant (pss-93) lacking phosphatidylethanolamine (PE). Several properties of this mutant are comparable to phenotypes of cpxA point mutants, indicating that this two-component pathway is activated in PE-deficient cells. In con...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.4.1029-1034.1997
更新日期:1997-02-01 00:00:00
abstract::This study examined the catabolism of xanthone by an Arthrobacter sp. (strain GFB100) capable of growth on xanthone as its main source of carbon and energy. An early catabolic intermediate was 3,4-dihydroxyxanthone. This compound was isolated from the growth medium of a mutant strain of the Arthrobacter sp. which lack...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.167.3.818-827.1986
更新日期:1986-09-01 00:00:00
abstract::A set of oligonucleotide primers capable of initiating enzymatic amplification (polymerase chain reaction) on a phylogenetically and taxonomically wide range of bacteria is described along with methods for their use and examples. One pair of primers is capable of amplifying nearly full-length 16S ribosomal DNA (rDNA) ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.2.697-703.1991
更新日期:1991-01-01 00:00:00
abstract::Assays of CDP-glycerol:poly(glycerophosphate) glycerophosphotransferase (CGPTase) (EC 2.7.8.12) in membranes isolated from Bacillus subtilis 168 wild type and 11 strains bearing conditional lethal thermosensitive mutations in tagB, tagD, or tagF revealed that CGPTase deficiency was associated only with mutant tagF all...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.2.646-649.1992
更新日期:1992-01-01 00:00:00
abstract::Nishida, Shoki (Kanazawa University, Kanazawa, Japan), and Gizo Nakagawara. Relationship between toxigenicity and sporulating potency of Clostridium novyi. J. Bacteriol. 89:993-995. 1965.-The less toxigenic the strains, the stronger was the sporulating potency of Clostridium novyi strains isolated. This was confirmed ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.89.4.993-995.1965
更新日期:1965-04-01 00:00:00
abstract::The fermentative alcohol dehydrogenase of Escherichia coli is encoded by the adhE gene, which is induced under anaerobic conditions but repressed in air. Previous work suggested that induction of adhE might depend on NADH levels. We therefore directly measured the NAD+ and NADH levels for cultures growing aerobically ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.20.6013-6018.1996
更新日期:1996-10-01 00:00:00
abstract::The group A streptococcus (GAS) Streptococcus pyogenes is known to cause self-limiting purulent infections in humans. The role of GAS pili in host cell adhesion and biofilm formation is likely fundamental in early colonization. Pilus genes are found in the FCT (fibronectin-binding protein, collagen-binding protein, an...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.06283-11
更新日期:2012-02-01 00:00:00
abstract::A gene (aad) coding for an aldehyde/alcohol dehydrogenase (AAD) was identified immediately upstream of the previously cloned ctfA (J. W. Cary, D. J. Petersen, E. T. Papoutsakis, and G. N. Bennett, Appl. Environ. Microbiol. 56:1576-1583, 1990) of Clostridium acetobutylicum ATCC 824 and sequenced. The 2,619-bp aad codes...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.3.871-885.1994
更新日期:1994-02-01 00:00:00
abstract::A newly isolated bacterium, Pseudomonas geniculata N1, can efficiently degrade nicotine. Here we present a 4.51-Mb assembly of its genome, which is the first sequence of the P. geniculata group. The sequence contains the genes related to nicotine catabolism and may provide insights into its molecular mechanism for N-h...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00625-12
更新日期:2012-07-01 00:00:00
abstract::Among 30 conjugative plasmids of enteric bacteria from 23 incompatibility (Inc) groups, we found 19 (from 12 Inc groups) which can complement defects caused by a defective single-stranded DNA-binding protein of Escherichia coli K-12. The genes which are responsible for the complementation from three of these plasmids ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.162.1.235-241.1985
更新日期:1985-04-01 00:00:00
abstract::Sialidase activity varies widely among strains and tends to correlate with strain virulence in the avian pathogen Mycoplasma synoviae. To characterize the forms of selection acting on enzymes required for sialic acid scavenging and catabolism, the ratios of nonsynonymous (K(a)) to synonymous (K(s)) mutation frequency ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00142-09
更新日期:2009-06-01 00:00:00
abstract::The bacteriocin produced by a strain of Staphylococcus aureus has been isolated and designated staphylococcin (414), and a study was made of its chemical, physical, and biological properties. The staphylococcin is released in appreciable quantities after breakage of the cells and can be purified through differential c...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.104.1.117-125.1970
更新日期:1970-10-01 00:00:00
abstract::A Boivin preparation of Brucella abortus, unlike common enterobacterial endotoxins, failed to depress water intake or increase numbers of hemolysin-producing spleen cells in mice, or to cause delayed inflammatory reactions in rabbit skin. Reactivity to the B. abortus endotoxin was found only in animals which were prev...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.95.2.286-290.1968
更新日期:1968-02-01 00:00:00
abstract::We have constructed a suicide vector, pU1800, containing the transposable element TnphoA (Tn5 IS50L::phoA), for the purpose of producing protein fusions in vivo between the Escherichia coli alkaline phosphatase (APase) and proteins of the facultative photoheterotroph, Rhodobacter sphaeroides. We introduced TnphoA into...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.8.4385-4394.1989
更新日期:1989-08-01 00:00:00
abstract::The light organ of the squid Euprymna scolopes is specifically colonized to a high density by the marine bacterium Vibrio fischeri. To date, only a few factors contributing to the specificity of this symbiosis have been identified. Using a genetic screen for random transposon mutants defective in initiating the symbio...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.3.835-842.2001
更新日期:2001-02-01 00:00:00
abstract::Citrate metabolism in Leuconostoc mesenteroides subspecies mesenteroides is associated with the generation of a proton motive force by a secondary mechanism (C. Marty-Teysset, C. Posthuma, J. S. Lolkema, P. Schmitt, C. Divies, and W. N. Konings, J. Bacteriol. 178:2178-2185, 1996). The pathway consists of four steps: (...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.21.6209-6215.1996
更新日期:1996-11-01 00:00:00
abstract::Various methods of inducing autolysis of Escherichia coli cells were investigated, some being described here for the first time. For the autolysis of growing cells only induction methods interfering with the biosynthesis of peptidoglycan were taken into consideration, whereas with harvested cells autolysis was induced...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:
更新日期:1982-10-01 00:00:00
abstract::Glyoxal is toxic and mutagenic α-oxoaldehyde generated in vivo as an oxidation by-product of sugar metabolism. We selected glyoxal-resistant mutants from an Escherichia coli strain lacking major glyoxal-detoxifying genes, gloA and yqhD, by growing cells in medium containing a lethal concentration of glyoxal. The mutan...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.06062-11
更新日期:2012-04-01 00:00:00
abstract:UNLABELLED:Succinic semialdehyde (SSA), an important metabolite of γ-aminobutyric acid (GABA), is a ligand of the repressor AttJ regulating the expression of the attJ-attKLM gene cluster in the plant pathogen Agrobacterium tumefaciens. While the response of A. tumefaciens to GABA and the function of attKLM have been ex...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00373-15
更新日期:2016-01-11 00:00:00
abstract::Twelve strains of Agrobacterium radiobacter isolated from naturally occurring crown galls or soil were found to be avirulent on sunflower, tomato, Kalanchoe, and carrot. Eleven strains contained plasmids of molecular weights 77 X 10(6) to 182 X 10(6) as determined by electron microscopy. One strain contained only a sm...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.129.1.76-80.1977
更新日期:1977-01-01 00:00:00
abstract::The ftf gene, coding for the cell-bound beta-D-fructosyltransferase (FTF) of Streptococcus salivarius ATCC 25975, has been analyzed, and its deduced amino acid sequence has been compared with that of the secreted FTF of Streptococcus mutans and the levansucrases (SacBs) of Bacillus species. A unique proline-rich regio...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.14.4520-4527.1993
更新日期:1993-07-01 00:00:00
abstract::Eight metH mutants in Salmonella typhimurium with closely linked sites of mutation which could grow only on methionine were isolated from a metE mutant deficient in N(5)-methyltetrahydropteroyltriglutamate-homocysteine transmethylase; their deficiency in cobalamin-dependent N(5)-methyltetrahydrofolate-homocysteine tra...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.100.1.377-382.1969
更新日期:1969-10-01 00:00:00
abstract::In Escherichia coli, the phosphorylation and dephosphorylation of isocitrate dehydrogenase (IDH) are catalyzed by a bifunctional protein kinase/phosphatase. We have determined the nucleotide sequence of aceK, the gene encoding IDH kinase/phosphatase. This gene consists of a single open reading frame of 1,734 base pair...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.6.2763-2769.1988
更新日期:1988-06-01 00:00:00
abstract::We cloned and sequenced a fragment of the Bacillus stearothermophilus NUB36 chromosome that contains two open reading frames (ORFs) whose products were detected only in cells of cultures grown in complex medium at high temperature. The nucleotide sequence of the two ORFs exhibited significant identity to the sequence ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.15.4877-4888.1991
更新日期:1991-08-01 00:00:00
abstract::A procedure for protoplast formation in Escherichia coli is described. Removal of the cell wall was confirmed by examination of cells in thin-section preparations. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.128.2.668-670.1976
更新日期:1976-11-01 00:00:00
abstract::In vivo assembly of the Klebsiella aerogenes urease nickel metallocenter requires the presence of UreD, UreF, and UreG accessory proteins and is further facilitated by UreE. Prior studies had shown that urease apoprotein exists in an uncomplexed form as well as in a series of UreD-urease (I.-S. Park, M.B. Carr, and R....
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.18.5417-5421.1996
更新日期:1996-09-01 00:00:00
abstract::A new carboxypeptidase (carboxypeptidase S) was found in a Saccharomyces cerevisiae strain lacking carboxypeptidase Y (D. H. Wolf and U. Weiser, Eur. J. Biochem. 73:553-556, 1977). Mutants devoid of carboxypeptidase S activity were isolated from a mutant strain that was also deficient in carboxypeptidase Y. Four mutan...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.147.2.418-426.1981
更新日期:1981-08-01 00:00:00
abstract::Genetic diversity and relationships in 74 Helicobacter pylori isolates recovered from patients assigned to distinct clinical categories were estimated by examination of allelic variation in six genes encoding metabolic housekeeping enzymes by multilocus enzyme electrophoresis. Seventy-three distinct allele profiles, r...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.13.3934-3938.1996
更新日期:1996-07-01 00:00:00