Molecular cloning of the protocatechuate 4,5-dioxygenase genes of Pseudomonas paucimobilis.

abstract：:The Haemophilus influenzae Rd rec-1+ gene was cloned from a partial chromosomal digest into a plasmid vector as a 20-kilobase-pair (kbp) BstEII fragment and then subcloned. The smallest subclone with rec-1+ activity carried a 3.1-kbp EcoRI fragment. The identity of the rec-I gene in these clones was confirmed by trans...

journal_title：Journal of bacteriology

authors： Stuy JH

更新日期：1989-08-01 00:00:00

abstract：:The pathogenic bacterium Bacillus anthracis has become the subject of intense study as a result of its use in a bioterrorism attack in the United States in September and October 2001. Previous studies suggested that B. anthracis Ames Ancestor, the original Ames fully virulent plasmid-containing isolate, was the ideal ...

journal_title：Journal of bacteriology

authors： Ravel J,Jiang L,Stanley ST,Wilson MR,Decker RS,Read TD,Worsham P,Keim PS,Salzberg SL,Fraser-Liggett CM,Rasko DA

更新日期：2009-01-01 00:00:00

abstract：:Enterococcus faecalis is associated with a high proportion of nosocomial infections; however, little is known of the ability of this organism to proliferate in vivo. The ability of RNase B, a model glycoprotein with a single N-glycosylation site occupied by a family of high-mannose-type glycans (Man(5)- to Man(9)-GlcN...

journal_title：Journal of bacteriology

authors： Roberts G,Tarelli E,Homer KA,Philpott-Howard J,Beighton D

更新日期：2000-02-01 00:00:00

abstract：:In Neurospora crassa, histidine starvation of histidine mutants resulted in derepression of histidine, tryptophan, and arginine biosynthetic enzymes. The same tripartite derepression occurred in wild-type strain 74A when it was grown in medium supplemented with 3-amino-1,2,4-triazole, an inhibitor of histidine biosynt...

journal_title：Journal of bacteriology

authors： Carsiotis M,Jones RF,Wesseling AC

更新日期：1974-09-01 00:00:00

abstract：:The shufflon, a multiple DNA inversion system in the plasmid R64, consists of four DNA segments flanked and separated by seven 19-bp repeat sequences. Site-specific recombinations mediated by the rci product occur between each inverted repeat sequence, resulting in inversions of the four segments independently or in g...

journal_title：Journal of bacteriology

authors： Gyohda A,Funayama N,Komano T

更新日期：1997-03-01 00:00:00

abstract：:Purified L-asparaginase from Serratia marcescens had an apparent-weight average molecular weight of 171,000 to 180,000 as determined by electrophoresis on polyacrylamide gels and by sedimentation equilibrium at low speed in an analytical ultracentrifuge. A subunit molecular weight of 31,500 +/- 1,500 was estimated for...

journal_title：Journal of bacteriology

authors： Stern ML,Phillips AW,Gottlieb AJ

更新日期：1976-02-01 00:00:00

abstract：:A sequence-specific modification methylase (M . SinI) was isolated and purified from Escherichia coli harboring a derivative of recombinant plasmid pSI4 (see accompanying manuscript: C. Karreman and A. de Waard, J. Bacteriol. 170:2527-2532, 1988), which contains a Salmonella infantis DNA insert. The enzyme uniquely me...

journal_title：Journal of bacteriology

authors： Karreman C,de Waard A

更新日期：1988-06-01 00:00:00

abstract：:The nitrogenase proteins from eight organisms have been highly purified, and a survey of their cross-reactions shows that the nitrogenase proteins from a wide variety of organisms can interact with one another. An active cross-reaction is the complementary functioning of the MoFe protein and the Fe protein from differ...

journal_title：Journal of bacteriology

authors： Emerich DW,Burris RH

更新日期：1978-06-01 00:00:00

abstract：:Mild alkaline extraction of native poly-beta-hydroxybutyrate (PHB) granules results in the solubilization of a protein fraction. Both the solubilized protein fraction and the extracted granules are essentially devoid of PHB synthetase activity unless recombined. The protein fraction has been separated by chromatograph...

journal_title：Journal of bacteriology

authors： Griebel RJ,Merrick JM

更新日期：1971-11-01 00:00:00

abstract：:The coccoid cyanobacterium Chroococcidiopsis dominates microbial communities in the most extreme arid hot and cold deserts. These communities withstand constraints that result from multiple cycles of drying and wetting and/or prolonged desiccation, through mechanisms which remain poorly understood. Here we describe th...

journal_title：Journal of bacteriology

authors： Billi D,Friedmann EI,Helm RF,Potts M

更新日期：2001-04-01 00:00:00

abstract：:The antigenically related coaggregation receptor polysaccharides (RPS) of Streptococcus oralis strains C104 and SK144 mediate recognition of these bacteria by other members of the dental plaque biofilm community. In the present study, the structure of strain SK144 RPS was established by high resolution NMR spectroscop...

journal_title：Journal of bacteriology

authors： Yang J,Ritchey M,Yoshida Y,Bush CA,Cisar JO

更新日期：2009-03-01 00:00:00

abstract：:Genetic and segregation analysis of Escherichia coli strains containing a partial duplication of the trp operon reveal that the 2.5-min-long region trpD-purB is duplicated in tandem in the chromosome. The adjacent loci cysB and fabD are not duplicated. Although one copy of the duplicated region is longer than the maxi...

journal_title：Journal of bacteriology

authors： Simonian MH,Goldstein RV,Mosteller RD

更新日期：1978-02-01 00:00:00

abstract：:Paenibacillus mucilaginosus is a ubiquitous functional bacterium in microbial fertilizer. Here we report the complete sequence of P. mucilaginosus 3016. Multiple sets of functional genes have been found in the genome. To the best of our knowledge, this is the first announcement about the complete genome sequence of a ...

journal_title：Journal of bacteriology

authors： Ma M,Wang Z,Li L,Jiang X,Guan D,Cao F,Chen H,Wang X,Shen D,Du B,Li J

更新日期：2012-05-01 00:00:00

abstract：:Smith, Doyle C. (Kansas State University, Manhattan), V. D. Foltz, and T. H. Lord. Two lytic factors involved in induced synergistic hemolysis in normally nonhemolytic staphylococci. J. Bacteriol. 88:1700-1704. 1964.-Synergistic hemolysis of "normally nonhemolytic" staphylococci results from the combination of two fac...

journal_title：Journal of bacteriology

authors： SMITH DC,FOLTZ VD,LORD TH

更新日期：1964-12-01 00:00:00

abstract：:Dowell, C. E. (The University of Texas, Dallas) and E. D. Rosenblum. Staphylococcal transducing particle. J. Bacteriol. 84:1076-1079. 1962.-When novobiocin-resistant transductants were isolated under conditions that permitted superinfection, almost all the clones were lysogenic for the transducing phage. If superinfec...

journal_title：Journal of bacteriology

authors： Dowell CE,Rosenblum ED

更新日期：1962-11-01 00:00:00

abstract：:We isolated pili from Escherichia coli SS142. These pili had a diameter of 6 nm and an average length of 400 nm. They were composed of subunits with a molecular weight of 18,000. Their amino acid composition was determined; methionine and proline were not detected. The isolated pili retained mannose-resistant hemagglu...

journal_title：Journal of bacteriology

authors： Mett H,Kloetzlen L,Vosbeck K

更新日期：1983-02-01 00:00:00

abstract：:Functional domain structures of pyocins AP41, S1, and S2 were assigned by examining the functions of chimeric pyocins and deletion derivatives. Pyocins AP41, S1, and S2 are essentially composed of three domains, the receptor-binding domain, the translocation domain, and the DNase domain, in that order from the N termi...

journal_title：Journal of bacteriology

authors： Sano Y,Kobayashi M,Kageyama M

更新日期：1993-10-01 00:00:00

abstract：:After inoculation of a stationary-phase culture of Nitrosomonas europaea into fresh growth solution, the cell-associated orthophosphate increased rapidly to 800 mumoles/g (wet weight), whereas the acid-insoluble long-chain polyphosphate content decreased rapidly to 22 mumoles/g. As growth proceeded, the orthophosphate...

journal_title：Journal of bacteriology

authors： Terry KR,Hooper AB

更新日期：1970-07-01 00:00:00

abstract：:Certain oral treponemes express a highly proteolytic phenotype and have been associated with periodontal diseases. The periodontal pathogen Treponema denticola produces dentilisin, a serine protease of the subtilisin family. The two-gene operon prcA-prtP is required for expression of active dentilisin (PrtP), a putati...

journal_title：Journal of bacteriology

authors： Correia FF,Plummer AR,Ellen RP,Wyss C,Boches SK,Galvin JL,Paster BJ,Dewhirst FE

更新日期：2003-12-01 00:00:00

abstract：:A number of heterologous plasmid deoxyribonucleic acids (DNAs) coding for erythromycin, tylosin, lincomycin, tetracycline, or chloramphenicol resistance have been introduced into Streptococcus pneumoniae via genetic transformation with frequencies that varied between 10(-5) to as high as 5 x 10(-1) per colony-forming ...

journal_title：Journal of bacteriology

authors： Barany F,Tomasz A

更新日期：1980-11-01 00:00:00

abstract：:lac operon fusions to DNA damage-inducible (din) loci were generated in Salmonella typhimurium LT2. Many of these din fusions were efficiently repressed by cloned Escherichia coli LexA, while others were not; all required RecA for induction. Several din fusions exhibited strong inducibility and will be useful in devel...

journal_title：Journal of bacteriology

authors： Smith CM,Arany Z,Orrego C,Eisenstadt E

更新日期：1991-06-01 00:00:00

abstract：:The heat shock response of Bradyrhizobium japonicum is controlled by a complex network involving two known regulatory systems. While some heat shock genes are controlled by a highly conserved inverted-repeat structure (CIRCE), others depend on a sigma 32-type heat shock sigma factor. Using Western blot (immunoblot) an...

journal_title：Journal of bacteriology

authors： Narberhaus F,Weiglhofer W,Fischer HM,Hennecke H

更新日期：1996-09-01 00:00:00

abstract：:The general stress regulon of Bacillus subtilis comprises approximately 200 genes and is under the control of the alternative sigma factor σ(B). The activation of σ(B) occurs in response to multiple physical stress stimuli as well as energy starvation conditions. The expression of the general stress proteins provides ...

journal_title：Journal of bacteriology

authors： Reder A,Höper D,Gerth U,Hecker M

更新日期：2012-07-01 00:00:00

abstract：:A DNA fragment from Xanthomonas campestris pv. campestris that partially restored the carbohydrate fermentation pattern of a cya crp Escherichia coli strain was cloned and expressed in E. coli. The nucleotide sequence of this fragment revealed the presence of a 700-base-pair open reading frame that coded for a protein...

journal_title：Journal of bacteriology

authors： de Crecy-Lagard V,Glaser P,Lejeune P,Sismeiro O,Barber CE,Daniels MJ,Danchin A

更新日期：1990-10-01 00:00:00

abstract：:Diacyl phthiocerol esters and their congeners are mycobacterial virulence factors. The biosynthesis of these complex lipids remains poorly understood. Insight into their biosynthesis will aid the development of rationally designed drugs that inhibit their production. In this study, we investigate a biosynthetic step r...

journal_title：Journal of bacteriology

authors： Onwueme KC,Vos CJ,Zurita J,Soll CE,Quadri LE

更新日期：2005-07-01 00:00:00

abstract：:The mecA-27r gene, which encodes PBP2a-27r, was modified by site-specific mutagenesis, resulting in replacement of the N-terminal membrane anchor with a short chelating peptide (CP-PBP2a-27r). CP-PBP2a-27r retained the same binding affinity for beta-lactam antibiotics as the wild-type enzyme. Approximately 95% pure CP...

journal_title：Journal of bacteriology

authors： Wu CY,Blaszczak LC,Smith MC,Skatrud PL

更新日期：1994-03-01 00:00:00

abstract：:ATP-dependent glucokinase is suggested to have evolved from a hypothetical polyphosphate (polyP)-dependent glucokinase (polyP-GK) via a bifunctional polyP/ATP glucokinase (polyP/ATP-GK). Here we showed that polyP-GK is present in a polyP-accumulating bacterium, Microlunatus phosphovorus. The polyP-GK produced glucose-...

journal_title：Journal of bacteriology

authors： Tanaka S,Lee SO,Hamaoka K,Kato J,Takiguchi N,Nakamura K,Ohtake H,Kuroda A

更新日期：2003-09-01 00:00:00

abstract：:Lyons, A. J., Jr. (Northern Regional Research Laboratory, Peoria, Ill.) and T. G. Pridham. Proposal to designate strain ATCC 3004 (IMRU 3004) as the neotype strain of Streptomyces albus (Rossi-Doria) Waksman and Henrici. J. Bacteriol. 83:370-380. 1962.-It is proposed that strain ATCC 3004 (IMRU 3004) be designated hen...

journal_title：Journal of bacteriology

authors： LYONS AJ Jr,PRIDHAM TG

更新日期：1962-02-01 00:00:00

abstract：:The genetic context, phylogeny, and biochemistry of a gene flanking the H(2)-forming methylene-H(4)-methanopterin dehydrogenase gene (hmdA), here designated hmdB, indicate that it is a new member of the radical S-adenosylmethionine enzyme superfamily. In contrast to the characteristic CX(3)CX(2)C or CX(2)CX(4)C motif ...

journal_title：Journal of bacteriology

authors： McGlynn SE,Boyd ES,Shepard EM,Lange RK,Gerlach R,Broderick JB,Peters JW

更新日期：2010-01-01 00:00:00

abstract：:Successful completion of the bacteriophage P2 lytic cycle requires phage-induced lysis of its Escherichia coli host, a process that is poorly understood. Genetic analysis of lysis-deficient mutants defined a single locus, gene K, which lies within the largest late transcription unit of P2 and maps between head gene L ...

journal_title：Journal of bacteriology

authors： Ziermann R,Bartlett B,Calendar R,Christie GE

更新日期：1994-08-01 00:00:00