Abstract:
:The brucellae are the etiological agents of brucellosis, a worldwide-distributed zoonosis. These bacteria are facultative intracellular parasites and thus are able to adjust their metabolism to the extra- and intracellular environments encountered during an infectious cycle. However, this aspect of Brucella biology is imperfectly understood, and the nutrients available in the intracellular niche are unknown. Here, we investigated the central pathways of C metabolism used by Brucella abortus by deleting the putative fructose-1,6-bisphosphatase (fbp and glpX), phosphoenolpyruvate carboxykinase (pckA), pyruvate phosphate dikinase (ppdK), and malic enzyme (mae) genes. In gluconeogenic but not in rich media, growth of ΔppdK and Δmae mutants was severely impaired and growth of the double Δfbp-ΔglpX mutant was reduced. In macrophages, only the ΔppdK and Δmae mutants showed reduced multiplication, and studies with the ΔppdK mutant confirmed that it reached the replicative niche. Similarly, only the ΔppdK and Δmae mutants were attenuated in mice, the former being cleared by week 10 and the latter persisting longer than 12 weeks. We also investigated the glyoxylate cycle. Although aceA (isocitrate lyase) promoter activity was enhanced in rich medium, aceA disruption had no effect in vitro or on multiplication in macrophages or mouse spleens. The results suggest that B. abortus grows intracellularly using a limited supply of 6-C (and 5-C) sugars that is compensated by glutamate and possibly other amino acids entering the Krebs cycle without a critical role of the glyoxylate shunt.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Zúñiga-Ripa A,Barbier T,Conde-Álvarez R,Martínez-Gómez E,Palacios-Chaves L,Gil-Ramírez Y,Grilló MJ,Letesson JJ,Iriarte M,Moriyón Idoi
10.1128/JB.01663-14subject
Has Abstractpub_date
2014-08-15 00:00:00pages
3045-57issue
16eissn
0021-9193issn
1098-5530pii
JB.01663-14journal_volume
196pub_type
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更新日期:1981-06-01 00:00:00
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journal_title:Journal of bacteriology
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更新日期:1966-10-01 00:00:00
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pub_type: 杂志文章
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更新日期:1986-07-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2004-09-01 00:00:00
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更新日期:2000-08-01 00:00:00
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更新日期:1969-09-01 00:00:00
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更新日期:1968-09-01 00:00:00
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更新日期:1966-02-01 00:00:00
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journal_title:Journal of bacteriology
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更新日期:1961-12-01 00:00:00
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pub_type: 杂志文章
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更新日期:1987-01-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1998-07-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2005-01-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.85.4.895-900.1963
更新日期:1963-04-01 00:00:00
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pub_type: 杂志文章
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更新日期:2011-10-01 00:00:00