Stringent control of peptidoglycan biosynthesis in Escherichia coli K-12.

Abstract:

:[3H]Diaminopimelic acid (Dap) was incorporated exclusively into peptidoglycan by Escherichia coli strains auxotrophic for both lysine and Dap. The rate of [3H]Dap incorporation by stringent (rel+) strains was significantly decreased when cells were deprived of required amino acids. The addition of chloramphenicol to amino acid-starved rel+ cultured stimulated both peptidoglycan and ribonucleic acid synthesis. In contrast, a relaxed (relA) derivative incorporated [3H]Dap at comparable rates in the presence or absence of required amino acids. Physiologically significant concentrations of guanosine 5'-diphosphate 3'-diphosphate (ppGpp) inhibited the in vitro synthesis of both carrier lipid-linked intermediate and peptidoglycan catalyzed by a particulate enzyme system. The degree of inhibition was dependent on the concentration of ppGpp in the reaction mixture. Thus, the results of in vivo and in vitro studies indicate that peptidoglycan synthesis is stringently controlled in E. coli.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Ishiguro EE,Ramey WD

doi

10.1128/JB.127.3.1119-1126.1976

subject

Has Abstract

pub_date

1976-09-01 00:00:00

pages

1119-26

issue

3

eissn

0021-9193

issn

1098-5530

journal_volume

127

pub_type

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