Buoyant density, growth rate, and the cell cycle in Streptococcus faecium.

Abstract:

:The buoyant density in rapidly growing Streptococcus faecium 9790 cells varies over the cell cycle, in contrast to the density in Escherichia coli. Buoyant density in S. faecium was measured by using Percoll (Pharmacia Fine Chemicals, Piscataway, N.J.) density gradients. We found that the mean and coefficient of variation of the population density increased with growth rate; and within a population, the mean cell volume, which was measured electronically, increased with density. These results were compared with electron microscopic measurements of the size distributions of cell wall growth sites within each fraction of the density gradient. As the density increased within a population, the frequency of large cells increased and the frequency of newly initiated cell wall growth sites increased. These effects were more marked as the growth rate increased. Next, these data were regrouped by cell size by using the size of the central growth site as an index of cell cycle stage. Each frequency value was weighted by the proportion of the population represented by that density fraction. Then, the average buoyant density was calculated for each value of cell size. In all cell populations, the density decreased and then increased as the central site enlarged. Peripheral growth sites were initiated as density reached a maximum. At faster growth rates, density increased more steeply, and new peripheral growth sites opened up at a higher frequency. We suggest that the rate at which density increases during the cell cycle correlates with the initiation of new cell wall growth sites.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Glaser D,Higgins M

doi

10.1128/jb.171.2.669-673.1989

subject

Has Abstract

pub_date

1989-02-01 00:00:00

pages

669-73

issue

2

eissn

0021-9193

issn

1098-5530

journal_volume

171

pub_type

杂志文章
  • BIOTIN BIOSYNTHESIS. I. BIOTIN YIELDS AND BIOTIN VITAMERS IN CULTURES OF PHYCOMYCES BLAKESLEEANUS.

    abstract::Eisenberg, M. A. (Columbia University, New York, N.Y.). Biotin biosynthesis. I. Biotin yields and biotin vitamers in cultures of Phycomyces blakesleeanus. J. Bacteriol. 86:673-680. 1963.-The addition of pimelic acid to a well-aerated medium resulted in a 10- to 12-fold increase in the biotin production of Phycomyces b...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.86.4.673-680.1963

    authors: EISENBERG MA

    更新日期:1963-10-01 00:00:00

  • A dominant sulfhydryl-containing protein in the outer membrane of Neisseria gonorrhoeae.

    abstract::By using a method that labels sulfhydryl-containing proteins in situ, we have detected a major outer membrane protein of Neisseria gonorrhoeae at 41 kDa. A protein of this molecular mass has not previously been shown to be a major outer membrane protein in gonococci. In addition, a minor protein rich in cysteinyl resi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.4.1173-1175.1993

    authors: Norrod EP,Browne SL,Feldweg A,Leonard J

    更新日期:1993-02-01 00:00:00

  • Specialized transduction with lambda plac5: dependence on recB.

    abstract::Genetically disabled lambda plac5 transducing phage derivatives were used to study the recB dependence of recombination during specialized transduction. The frequency of transduction was normalized to colony-forming units, and the end product of recombination was monitored by scoring for addition and substitution tran...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.150.3.1485-1488.1982

    authors: Porter RD,Welliver RA,Witkowski TA

    更新日期:1982-06-01 00:00:00

  • Identification and Characterization of the Major Porin of Desulfovibrio vulgaris Hildenborough.

    abstract::Due in large part to their ability to facilitate the diffusion of a diverse range of solutes across the outer membrane (OM) of Gram-negative bacteria, the porins represent one of the most prominent and important bacterial membrane protein superfamilies. Notably, for the Gram-negative bacterium Desulfovibrio vulgaris H...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00286-17

    authors: Zeng L,Wooton E,Stahl DA,Walian PJ

    更新日期:2017-10-31 00:00:00

  • Alpha-alpha'-dipyridyl or ortho-phenanthroline stimulation of the soluble reduced nicotinamide adenine dinucleotide oxidase from Bacillus subtilis spores and dipicolinic acid inhibition of the stimulated enzymes.

    abstract::Soluble reduced nicotinamide adenine dinucleotide oxidase activity in extracts of Bacillus subtilis spores was stimulated by the addition of not only flavine mononucleotide (FMN) or flavine adenine dinucleotide (FAD) but also alpha-alpha'-dipyridyl or o-phenanthroline. These chelating agents showed stronger effect on ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.117.3.1017-1022.1974

    authors: Tochikubo K

    更新日期:1974-03-01 00:00:00

  • Lon Protease Removes Excess Signal Recognition Particle Protein in Escherichia coli.

    abstract::Correct targeting of membrane proteins is essential for membrane integrity, cell physiology, and viability. Cotranslational targeting depends on the universally conserved signal recognition particle (SRP), which is a ribonucleoprotein complex comprised of the protein component Ffh and the 4.5S RNA in Escherichia coli ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00161-20

    authors: Sauerbrei B,Arends J,Schünemann D,Narberhaus F

    更新日期:2020-06-25 00:00:00

  • Heterologous expression and purification of active divercin V41, a class IIa bacteriocin encoded by a synthetic gene in Escherichia coli.

    abstract::Divercin V41, a class IIa bacteriocin with strong antilisterial activity, is produced by Carnobacterium divergens V41. To express a recombinant version of divercin V41, we constructed a synthetic gene that encodes the mature divercin V41 peptide and then overexpressed the gene in pET-32b by using the T7 RNA polymerase...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.186.13.4276-4284.2004

    authors: Richard C,Drider D,Elmorjani K,Marion D,Prévost H

    更新日期:2004-07-01 00:00:00

  • Divergence among genes encoding the elongation factor Tu of Yersinia Species.

    abstract::Elongation factor Tu (EF-Tu), encoded by tuf genes, carries aminoacyl-tRNA to the ribosome during protein synthesis. Duplicated tuf genes (tufA and tufB), which are commonly found in enterobacterial species, usually coevolve via gene conversion and are very similar to one another. However, sequence analysis of tuf gen...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01067-08

    authors: Isabel S,Leblanc E,Boissinot M,Boudreau DK,Grondin M,Picard FJ,Martel EA,Parham NJ,Chain PS,Bader DE,Mulvey MR,Bryden L,Roy PH,Ouellette M,Bergeron MG

    更新日期:2008-11-01 00:00:00

  • Two novel membrane proteins, TcpD and TcpE, are essential for conjugative transfer of pCW3 in Clostridium perfringens.

    abstract::The anaerobic pathogen Clostridium perfringens encodes either toxin genes or antibiotic resistance determinants on a unique family of conjugative plasmids that have a novel conjugation region, the tcp locus. Studies of the paradigm conjugative plasmid from C. perfringens, the 47-kb tetracycline resistance plasmid pCW3...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.02466-14

    authors: Wisniewski JA,Teng WL,Bannam TL,Rood JI

    更新日期:2015-02-15 00:00:00

  • Magnesium starvation of Aerobacter aerogenes. II. Rates of nucleic acid synthesis and methods for their measurement.

    abstract::The rates of synthesis of Aerobacter aerogenes nucleic acids were estimated during incubation of the bacteria in a Mg(++)-free medium. Deoxyribonucleic acid (DNA) synthesized during Mg(++) starvation, or in the preceding exponential growth, remained acid-precipitable for 2.5 hr before breaking down to acid-soluble pro...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.93.1.345-356.1967

    authors: Kennell D,Kotoulas A

    更新日期:1967-01-01 00:00:00

  • Substrate specificity of naphthalene dioxygenase: effect of specific amino acids at the active site of the enzyme.

    abstract::The three-component naphthalene dioxygenase (NDO) enzyme system carries out the first step in the aerobic degradation of naphthalene by Pseudomonas sp. strain NCIB 9816-4. The three-dimensional structure of NDO revealed that several of the amino acids at the active site of the oxygenase are hydrophobic, which is consi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.182.6.1641-1649.2000

    authors: Parales RE,Lee K,Resnick SM,Jiang H,Lessner DJ,Gibson DT

    更新日期:2000-03-01 00:00:00

  • Yield coefficients of Thiobacillus neapolitanus in continuous culture.

    abstract::Thiobacillus neapolitanus, when grown in continuous culture with thiosulfate limiting growth, possessed an apparent maximal molar growth yield of 8.0 g (dry weight) per mole of thiosulfate. The substrate requirement for energy of maintenance was the highest yet reported, amounting to 21.8 mmoles of thiosulfate per g p...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.93.3.874-878.1967

    authors: Hempfling WP,Vishniac W

    更新日期:1967-03-01 00:00:00

  • Influences of various amino acids on tryptophan-mediated control of the tryptophan biosynthetic enzymes in Escherichia coli.

    abstract::Lysates of Escherichia coli Ymel obtained from cultures grown in the absence of tryptophan in minimal medium supplemented with 0.1% casein hydrolysate show an approximate fivefold increase in steady-state specific activity of both anthranilate synthetase and tryptophan synthetase A protein relative to cultures grown i...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.108.3.1181-1191.1971

    authors: Stubbs JD,Stubbs EA

    更新日期:1971-12-01 00:00:00

  • IS1549 from Mycobacterium smegmatis forms long direct repeats upon insertion.

    abstract::A new insertion element, IS1549, was identified serendipitously from Mycobacterium smegmatis LR222 during experiments using a vector designed to detect the excision of IS6110 from between the promoter region and open reading frame (ORF) of an aminoglycoside phosphotransferase gene. Six of the kanamycin-resistant isola...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.5.1037-1043.1998

    authors: Plikaytis BB,Crawford JT,Shinnick TM

    更新日期:1998-03-01 00:00:00

  • Localization of P42 and F(1)-ATPase α-subunit homolog of the gliding machinery in Mycoplasma mobile revealed by newly developed gene manipulation and fluorescent protein tagging.

    abstract::Mycoplasma mobile has a unique mechanism that enables it to glide on solid surfaces faster than any other gliding mycoplasma. To elucidate the gliding mechanism, we developed a transformation system for M. mobile based on a transposon derived from Tn4001. Modification of the electroporation conditions, outgrowth time,...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01418-13

    authors: Tulum I,Yabe M,Uenoyama A,Miyata M

    更新日期:2014-05-01 00:00:00

  • DNA supercoiling and suppression of the leu-500 promoter mutation.

    abstract::top mutations (formerly supX) eliminate DNA topoisomerase I activity and suppress the leu-500 promoter mutation in Salmonella typhimurium (K. M. Overbye, S. K. Basu, and P. Margolin, Cold Spring Harbor Symp. Quant. Biol. 47:785-791, 1983). Sublethal doses of coumermycin which reduce intracellular levels of supercoilin...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.164.2.947-949.1985

    authors: Pruss GJ,Drlica K

    更新日期:1985-11-01 00:00:00

  • Structure and biological activities of beta toxin from Staphylococcus aureus.

    abstract::Beta toxin is a neutral sphingomyelinase secreted by certain strains of Staphylococcus aureus. This virulence factor lyses erythrocytes in order to evade the host immune system as well as scavenge nutrients. The structure of beta toxin was determined at 2.4-A resolution using crystals that were merohedrally twinned. T...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00741-07

    authors: Huseby M,Shi K,Brown CK,Digre J,Mengistu F,Seo KS,Bohach GA,Schlievert PM,Ohlendorf DH,Earhart CA

    更新日期:2007-12-01 00:00:00

  • Identification of a protein of 15,000 daltons related to isoleucine-valine biosynthesis in Escherichia coli K-12.

    abstract::The effect of the ilvG671, ilvG468, and ilvG603 mutations (phenotype, IlvG+ Valr; formerly ilvO) upon proteins synthesized was determined by infection of irradiated Escherichia coli K-12 cells, using specifically constructed derivatives of lambda dilv phage. These ilvG alleles are similar to the previously studied ilv...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.151.1.127-134.1982

    authors: Gray JE,Wallen JW,Calhoun DH

    更新日期:1982-07-01 00:00:00

  • Characterization of the antigenic subunits of the envelope protein of Yersinia pestis.

    abstract::Chemical, physical, and immunological properties of the envelope antigen of Yersinia pestis strains have been investigated. The antigen consists of two components with isoelectric points (pI) of 4.6 and 4.8. One component (pI 4.6) is a protein bound to a small carbohydrate moiety identified as an oligomeric galactan; ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.117.1.48-55.1974

    authors: Bennett LG,Tornabene TG

    更新日期:1974-01-01 00:00:00

  • Interference with valine and isoleucine biosynthesis by cyclic hydroxamic acids.

    abstract::Hogg, Robert W. (University of Illinois, Urbana), Chitra S. Biswas, and Harry P. Broquist. Interference with valine and isoleucine biosynthesis by cyclic hydroxamic acids. J. Bacteriol. 90:1265-1270. 1965.-The introduction of a hydroxyl group in a number of common barbiturates, a substitution which converts these comp...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.90.5.1265-1270.1965

    authors: Hogg RW,Biswas CS,Broquist HP

    更新日期:1965-11-01 00:00:00

  • Effects of single-strand DNases ExoI, RecJ, ExoVII, and SbcCD on homologous recombination of recBCD+ strains of Escherichia coli and roles of SbcB15 and XonA2 ExoI mutant enzymes.

    abstract::To assess the contributions of single-strand DNases (ssDNases) to recombination in a recBCD+ background, we studied 31 strains with all combinations of null alleles of exonuclease I (delta xon), exonuclease VII (xseA), RecJ DNase (recJ), and SbcCD DNase (sbcCD) and exonuclease I mutant alleles xonA2 and sbcB15. The xs...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01052-07

    authors: Thoms B,Borchers I,Wackernagel W

    更新日期:2008-01-01 00:00:00

  • Proton circulation in Vibrio costicola.

    abstract::The importance of proton movements was assessed in the moderate halophile Vibrio costicola. When anaerobic cells in acidic buffer (pH 6.5) were given an O2 pulse, protons were extruded regardless of the presence of Na+. At pH 8.5, however, V. costicola produced an acidic response to an O2 pulse in the absence of Na+ a...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.161.2.681-686.1985

    authors: Hamaide F,Kushner DJ,Sprott GD

    更新日期:1985-02-01 00:00:00

  • INTRACELLULAR BEHAVIOR OF HISTOPLASMA CAPSULATUM.

    abstract::Howard, Dexter H. (University of California, Los Angeles). Intracellular behavior of Histoplasma capsulatum. J. Bacteriol. 87:33-38. 1964.-The tinctorial characteristics and intracellular generation time of Histoplasma capsulatum within mouse histiocytes maintained in tissue culture were studied. The appearance of via...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.87.1.33-38.1964

    authors: HOWARD DH

    更新日期:1964-01-01 00:00:00

  • Interaction of native and mutant MecI repressors with sequences that regulate mecA, the gene encoding penicillin binding protein 2a in methicillin-resistant staphylococci.

    abstract::Methicillin resistance in staphylococci is mediated by PBP2a, a penicillin binding protein with low affinity for beta-lactam antibiotics. The gene encoding PBP2a, mecA, is transcriptionally regulated in some clinical isolates by mecR1 and mecI, genes divergently transcribed from mecA that encode a signal transducer an...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.8.2160-2166.1998

    authors: Sharma VK,Hackbarth CJ,Dickinson TM,Archer GL

    更新日期:1998-04-01 00:00:00

  • Influence of lipid components of Mycoplasma laidlawii membranes on osmotic fragility of cells.

    abstract::Razin, S. (University of Connecticut, Storrs), M. E. Tourtellotte, R. N. McElhaney, and J. D. Pollack. Influence of lipid components of Mycoplasma laidlawii membranes on osmotic fragility of cells. J. Bacteriol. 91:609-616. 1966.-Lipid composition of Mycoplasma laidlawii membranes could be significantly changed by var...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.91.2.609-616.1966

    authors: Razin S,Tourtellotte ME,McElhaney RN,Pollack JD

    更新日期:1966-02-01 00:00:00

  • Expression of a uniquely regulated extracellular polysaccharide confers a large-capsule phenotype to Bacteroides fragilis.

    abstract::Bacteroides fragilis synthesizes eight distinct capsular polysaccharides, more than any described bacterium outside the order Bacteroidales. Here, we show that this organism also produces a high-molecular-weight extracellular polysaccharide (EPS). Expression of the EPS results in the formation of a large polysaccharid...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01519-07

    authors: Chatzidaki-Livanis M,Coyne MJ,Roche-Hakansson H,Comstock LE

    更新日期:2008-02-01 00:00:00

  • Reductive activation of the methyl coenzyme M methylreductase system of Methanobacterium thermoautotrophicum delta H.

    abstract::When titanium(III) citrate was used as electron donor for the reduction of methyl coenzyme M by the methyl coenzyme M methylreductase system of Methanobacterium thermoautotrophicum delta H, component A1 was no longer required. The simpler system thus obtained required components A2, A3, and C as well as catalytic amou...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.170.9.3946-3952.1988

    authors: Rouvière PE,Bobik TA,Wolfe RS

    更新日期:1988-09-01 00:00:00

  • Pigment production from tryptophan by an Achromobacter species.

    abstract::Duerre, John A. (University of North Dakota, Grand Forks), and Patrick J. Buckley. Pigment production from tryptophan by an Achromobacter species. J. Bacteriol. 90:1686-1691. 1965.-A microorganism was isolated from the soil near the University of North Dakota. Biochemical and morphological characteristics indicated th...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.90.6.1686-1691.1965

    authors: Duerre JA,Buckley PJ

    更新日期:1965-12-01 00:00:00

  • Multifaceted Interfaces of Bacterial Competition.

    abstract::Microbial communities span many orders of magnitude, ranging in scale from hundreds of cells on a single particle of soil to billions of cells within the lumen of the gastrointestinal tract. Bacterial cells in all habitats are members of densely populated local environments that facilitate competition between neighbor...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章,评审

    doi:10.1128/JB.00275-16

    authors: Stubbendieck RM,Straight PD

    更新日期:2016-07-28 00:00:00

  • Fructose 1,6-bisphosphate aldolase activity is essential for synthesis of alginate from glucose by Pseudomonas aeruginosa.

    abstract::We have isolated a mutant of Pseudomonas aeruginosa deficient in fructose 1,6-bisphosphate aldolase activity. This mutant, similar to the mutants deficient in any of the Entner-Doudoroff pathway enzymes, does not allow appreciable alginate formation from glucose and gluconate, but allows alginate synthesis from mannit...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.161.1.458-460.1985

    authors: Banerjee PC,Vanags RI,Chakrabarty AM,Maitra PK

    更新日期:1985-01-01 00:00:00