Deoxyribonucleic acid sequence organization of a yeast plasmid.

Abstract:

:Two-micrometer deoxyribonucleic acid (DNA), a circular plasmid of Saccharomyces cerevisiae, contains two nontandem repeated sequences which are inverted with respect to one another. These repeated sequences together account for 21% of the molecule length. Restriction endonuclease analysis and electron microscopy demonstrated the existence of two forms of 2-mum DNA differing in the orientation of the interstitial segments bounded by the inverted repeated sequences. The two forms of 2-mum DNA could result from an intramolecular reciprocal recombination between inverted repeat elements. A map containing the restriction endonuclease sites and the units of the inverted repeat has been constructed.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Livingston DM,Klein HL

doi

10.1128/JB.129.1.472-481.1977

subject

Has Abstract

pub_date

1977-01-01 00:00:00

pages

472-81

issue

1

eissn

0021-9193

issn

1098-5530

journal_volume

129

pub_type

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