Polymer-Producing Species of Arthrobacter.

abstract：:Surface protein antigens of Mycoplasma hyopneumoniae were identified by direct antibody-surface binding or by radioimmunoprecipitation of surface 125I-labeled proteins with a series of monoclonal antibodies (MAbs). Surface proteins p70, p65, p50, and p44 were shown to be integral membrane components by selective parti...

journal_title：Journal of bacteriology

authors： Wise KS,Kim MF

更新日期：1987-12-01 00:00:00

abstract：:Growth of a derivative of Escherichia coli K-10 was strongly inhibited by 2 times 10(-4) M L-5(1-amino-2-methylbutyl)-tetrazole (isoleucine tetrazole). Growth inhibition was reversed by isoleucine, threonine, glycyl-L-isoleucine, or glycyl-L-threonine, and, in a valine-resistant mutant, by L-valine. Partial reversal o...

journal_title：Journal of bacteriology

authors： Willshaw GA,Tristram H

更新日期：1975-09-01 00:00:00

abstract：:The cytoplasmic accumulation of exogenous betaine stimulates the growth of Lactococcus lactis cultivated under hyperosmotic conditions. We report that L. lactis possesses a single betaine transport system that belongs to the ATP-binding cassette (ABC) superfamily of transporters. Through transposon mutagenesis, a muta...

journal_title：Journal of bacteriology

authors： Obis D,Guillot A,Gripon JC,Renault P,Bolotin A,Mistou MY

更新日期：1999-10-01 00:00:00

abstract：:The gene encoding a 45-kDa protein (45K) of Chlamydia trachomatis serovar F was cloned, sequenced, and overexpressed in Escherichia coli. Alignment of the deduced peptide sequence with E. coli elongation factor Tu (EF-Tu) demonstrated 69% identity. The 45K was recognized by a Chlamydia genus-specific monoclonal antibo...

journal_title：Journal of bacteriology

authors： Zhang YX,Shi Y,Zhou M,Petsko GA

更新日期：1994-02-01 00:00:00

abstract：:We report that transformation of Streptomyces lividans with cloned DNA of the SLP1 genetic element results in integration of the element at the same chromosomal locus (attB) normally occupied by SLP1 in its original host, Streptomyces coelicolor, and in S. lividans that has received SLP1 by mating. We constructed SLP1...

journal_title：Journal of bacteriology

authors： Omer CA,Stein D,Cohen SN

更新日期：1988-05-01 00:00:00

abstract：:Mutations in the araC gene of Escherichia coli B/r were isolated which alter both activation of the araBAD operon expression and autoregulation. The mutations were isolated on an araC-containing plasmid by hydroxylamine mutagenesis of plasmid DNA. The mutant phenotype selected was the inability to autoregulate. The DN...

journal_title：Journal of bacteriology

authors： Cass LG,Wilcox G

更新日期：1986-06-01 00:00:00

abstract：:Acetosyringone, a phenolic inducer of the virulence (vir) genes of Agrobacterium tumefaciens, inhibited the growth of the nopaline-type strains T37 and C58 incubated under acidic conditions. In the course of a 6-day incubation with acetosyringone, avirulent clones were produced in different proportions by strains T37 ...

journal_title：Journal of bacteriology

authors： Fortin C,Nester EW,Dion P

更新日期：1992-09-01 00:00:00

abstract：:Pseudomonas aeruginosa and many other bacteria can utilize biogenic polyamines, including diaminopropane (DAP), putrescine (Put), cadaverine (Cad), and spermidine (Spd), as carbon and/or nitrogen sources. Transcriptome analysis in response to exogenous Put and Spd led to the identification of a list of genes encoding ...

journal_title：Journal of bacteriology

authors： Yao X,He W,Lu CD

更新日期：2011-08-01 00:00:00

abstract：:Seven mutants of Haemophilus influenzae were isolated by the criterion of sensitivity to ultraviolet (UV) inactivation of colony formation. These mutants and the wild type were characterized with regard to X-ray inactivation of colony formation, UV induction of division inhibition, the ability of the eight strains to ...

journal_title：Journal of bacteriology

authors： Setlow JK,Brown DC,Boling ME,Mattingly A,Gordon MP

更新日期：1968-02-01 00:00:00

abstract：:C(1), a lytic bacteriophage infecting group C streptococci, is one of the earliest-isolated phages, and the method of bacterial classification known as phage typing was defined by using this bacteriophage. We present for the first time a detailed analysis of this phage by use of electron microscopy, protein profiling,...

journal_title：Journal of bacteriology

authors： Nelson D,Schuch R,Zhu S,Tscherne DM,Fischetti VA

更新日期：2003-06-01 00:00:00

abstract：:Methanohalophilus strain Z7302 was previously isolated from a hypersaline environment and grows over a range of NaCl concentrations from 1.7 to 4.4 M. We examined the relationships between cell growth rate, cell volume, and intracellular solute concentrations with increasing salinity. This extremely halophilic methano...

journal_title：Journal of bacteriology

authors： Lai MC,Gunsalus RP

更新日期：1992-11-01 00:00:00

abstract：:Phosphate acetyltransferase (PTA) and acetate kinase (AK) of the hyperthermophilic eubacterium Thermotoga maritima have been purified 1,500- and 250-fold, respectively, to apparent homogeneity. PTA had an apparent molecular mass of 170 kDa and was composed of one subunit with a molecular mass of 34 kDa, suggesting a h...

journal_title：Journal of bacteriology

authors： Bock AK,Glasemacher J,Schmidt R,Schönheit P

更新日期：1999-03-01 00:00:00

abstract：:Unique lipids found in Azotobacter vinelandii cysts are derived primarily from beta-hydroxybutyrate used to induce encystment. Tracer studies with beta-[14C]hydroxybutyrate showed that the biosynthesis of these compounds during encystment began at 8 to 12 h after induction and reached maximal levels after 2 days, Seve...

journal_title：Journal of bacteriology

authors： Su CJ,Sadoff HL

更新日期：1981-07-01 00:00:00

abstract：:Mycolic acids are a key component of the mycobacterial cell wall, providing structure and forming a major permeability barrier. In Mycobacterium tuberculosis mycolic acids are synthesized by type I and type II fatty acid synthases. One of the enzymes of the type II system is encoded by fabG1. We demonstrate here that ...

journal_title：Journal of bacteriology

authors： Parish T,Roberts G,Laval F,Schaeffer M,Daffé M,Duncan K

更新日期：2007-05-01 00:00:00

abstract：:The butyrogenic genes from Clostridium difficile DSM 1296(T) have been cloned and expressed in Escherichia coli. The enzymes acetyl-coenzyme A (CoA) C-acetyltransferase, 3-hydroxybutyryl-CoA dehydrogenase, crotonase, phosphate butyryltransferase, and butyrate kinase and the butyryl-CoA dehydrogenase complex composed o...

journal_title：Journal of bacteriology

authors： Aboulnaga el-H,Pinkenburg O,Schiffels J,El-Refai A,Buckel W,Selmer T

更新日期：2013-08-01 00:00:00

abstract：:The aquatic bacterium Caulobacter crescentus attaches to solid surfaces through an adhesive holdfast located at the tip of its polar stalk, a thin cylindrical extension of the cell membrane. In this paper, the elastic properties of the C. crescentus stalk and holdfast assembly were studied by using video light microsc...

journal_title：Journal of bacteriology

authors： Li G,Smith CS,Brun YV,Tang JX

更新日期：2005-01-01 00:00:00

abstract：:Two low-molecular-weight basic proteins, termed A and B proteins, comprise about 15% of the protein of dormant spores of Bacillus megaterium. Irradiation of intact dormant spores with ultraviolet light results in covalent cross-linking of the A and B proteins to other spore macromolecules. The cross-linked A and B pro...

journal_title：Journal of bacteriology

authors： Setlow B,Setlow P

更新日期：1979-08-01 00:00:00

abstract：:Relatedness in species of Erwinia was assessed by determining the extent of reassociation in heterologous deoxyribonucleic acid preparations. Thermal elution chromatography on hydroxyapatite was used to separate reassociated nucleotide sequences from nonreassociated sequences and to determine the thermal stability of ...

journal_title：Journal of bacteriology

authors： Brenner DJ,Fanning GR,Steigerwalt AG

更新日期：1972-04-01 00:00:00

abstract：:SigB is an alternative sigma factor that controls a large regulon in Staphylococcus aureus. Activation of SigB requires RsbU, a protein phosphatase 2C (PP2C)-type phosphatase. In a closely related organism, Bacillus subtilis, RsbU activity is stimulated upon interaction with RsbT, a kinase, which following an activati...

journal_title：Journal of bacteriology

authors： Pané-Farré J,Jonas B,Hardwick SW,Gronau K,Lewis RJ,Hecker M,Engelmann S

更新日期：2009-04-01 00:00:00

abstract：:Nakata, H. M. (Washington State University, Pullman). Effect of pH on intermediates produced during growth and sporulation of Bacillus cereus. J. Bacteriol. 86:577-581. 1963.-Cultures of Bacillus cereus strain T, grown in an unbuffered glucose-yeast extract-mineral salts medium and in the same medium buffered at pH 6....

journal_title：Journal of bacteriology

authors： NAKATA HM

更新日期：1963-09-01 00:00:00

abstract：:Lysyl-transfer ribonucleic acid synthetase (EC 6.1.1.6) was identified as four polypeptide spots after two-dimensional polyacrylamide gel electrophoresis of whole-cell lysates of Escherichia coli. Identification was made by migration with partially purified enzyme preparations, by peptide map patterns, by mutant analy...

journal_title：Journal of bacteriology

authors： Hirshfield IN,Bloch PL,Van Bogelen RA,Neidhardt FC

更新日期：1981-04-01 00:00:00

abstract：:The type III secretion system (T3SS) encoded by Salmonella pathogenicity island 2 (SPI-2) is involved in systemic infection and intracellular replication of Salmonella enterica serovar Typhimurium. In this study, we investigated the function of SsaE, a small cytoplasmic protein encoded within the SPI-2 locus, which sh...

journal_title：Journal of bacteriology

authors： Miki T,Shibagaki Y,Danbara H,Okada N

更新日期：2009-11-01 00:00:00

abstract：:The filamentous cyanobacterium Anabaena (Nostoc) sp. strain PCC 7120 produces specialized cells for nitrogen fixation called heterocysts. Previous work showed that the group 2 sigma factor sigE (alr4249; previously called sigF) is upregulated in differentiating heterocysts 16 h after nitrogen step-down. We now show th...

journal_title：Journal of bacteriology

authors： Mella-Herrera RA,Neunuebel MR,Kumar K,Saha SK,Golden JW

更新日期：2011-04-01 00:00:00

abstract：:The alkaline phosphatase (orthophosphoric monoester phosphydrolase, EC 3.1.3.1) of Bacillus licheniformis MC14 was studied in an attempt to determine the number of subunits contained in the 120,000-molecular-weight native enzyme. Two moles of arginine was liberated per mole of native enzyme by carboxypeptidases A and ...

journal_title：Journal of bacteriology

authors： Hulett FM,Schaffel SD,Campbell LL

更新日期：1976-11-01 00:00:00

abstract：:Temperature-sensitive nalA mutants of Escherichia coli have been used to investigate the structure and functions of deoxyribonucleic acid (DNA) gyrase. Extracts of one such mutant (nalA43) had thermosensitive DNA gyrase subunit A activity but normal gyrase subunit B activity, proving definitively that nalA is the stru...

journal_title：Journal of bacteriology

authors： Kreuzer KN,Cozzarelli NR

更新日期：1979-11-01 00:00:00

abstract：:Oligotropha carboxidovorans OM5(T) (DSM 1227, ATCC 49405) is a chemolithoautotrophic bacterium with the capability to utilize carbon monoxide, carbon dioxide, and hydrogen. It is also capable of heterotrophic growth under appropriate environmental conditions. Here we report the annotated genome sequence of the circula...

journal_title：Journal of bacteriology

authors： Paul D,Bridges S,Burgess SC,Dandass Y,Lawrence ML

更新日期：2008-08-01 00:00:00

abstract：:Krebs cycle enzyme activity in Bacillus subtilis was examined under aerobic and anaerobic conditions. Citrate synthase and aconitase activities in cells grown anaerobically in the presence of nitrate were reduced by as much as 10- and 30-fold, respectively, from levels observed under aerobic culture conditions. The ma...

journal_title：Journal of bacteriology

authors： Nakano MM,Zuber P,Sonenshein AL

更新日期：1998-07-01 00:00:00

abstract：:A procedure was developed for purification of ornithine transcarbamylase (OTCase) to near homogeneity from Bacillus subtilis 168. The purified native enzyme existed as a mixture of dimeric, tetrameric, and hexameric forms, but was converted to the dimer in the presence of 2-mercaptoethanol. The molecular weight of the...

journal_title：Journal of bacteriology

authors： Neway JO,Switzer RL

更新日期：1983-08-01 00:00:00

abstract：:Filaments of a thermosensitive Bacillus megaterium mutant showed an altered macromolecular composition compared with salt-cured mutant cells and parental cells. Filaments contained more peptidoglycan, polyglucose, poly-beta-hydroxy-butyrate, and deoxyribonucleic acid per unit of protein. The ribonucleic acid-to-protei...

journal_title：Journal of bacteriology

authors： Yan LP,Hitchins AD

更新日期：1980-10-01 00:00:00

abstract：:Nucleoside diphosphate kinase (Ndk) is a ubiquitous enzyme which functions in balancing the nucleotide pool of the cell. We have recently reported that in addition to being intracellular in both mucoid and nonmucoid Pseudomonas aeruginosa, Ndk is also secreted into the extracellular environment by mucoid P. aeruginosa...

journal_title：Journal of bacteriology

authors： Kamath S,Chen ML,Chakrabarty AM

更新日期：2000-07-01 00:00:00