Glycine betaine and potassium ion are the major compatible solutes in the extremely halophilic methanogen Methanohalophilus strain Z7302.

abstract：:Penicillin-binding protein 4a (PBP4a) from Bacillus subtilis was overproduced and purified to homogeneity. It clearly exhibits DD-carboxypeptidase and thiolesterase activities in vitro. Although highly isologous to the Actinomadura sp. strain R39 DD-peptidase (B. Granier, C. Duez, S. Lepage, S. Englebert, J. Dusart, O...

journal_title：Journal of bacteriology

authors： Duez C,Vanhove M,Gallet X,Bouillenne F,Docquier J,Brans A,Frère J

更新日期：2001-03-01 00:00:00

abstract：:Pathways of mannitol biosynthesis and utilization in Aspergillus candidus NRRL 305 were studied in cell-free extracts of washed mycelia prepared by sonic and French pressure cell treatments. A nicotinamide adenine dinucleotide-linked mannitol-1-phosphate (M1P) dehydrogenase was found in French pressure cell extracts o...

journal_title：Journal of bacteriology

authors： Strandberg GW

更新日期：1969-03-01 00:00:00

abstract：:The Rickettsia prowazekii sigma factor was overexpressed, purified, and used to reconstitute RNA polymerase holoenzyme species. R. prowazekii RNA polymerase-promoter complexes were unstable and remained dissociable and heparin sensitive under conditions in which the corresponding Escherichia coli complexes were not. T...

journal_title：Journal of bacteriology

authors： Aniskovitch LP,Winkler HH

更新日期：1995-11-01 00:00:00

abstract：:Many surface proteins of pathogenic gram-positive bacteria are linked to the cell wall envelope by a mechanism requiring a C-terminal sorting signal with an LPXTG motif. Surface proteins of Streptococcus pneumoniae harbor another motif, YSIRK-G/S, which is positioned within signal peptides. The signal peptides of some...

journal_title：Journal of bacteriology

authors： Bae T,Schneewind O

更新日期：2003-05-01 00:00:00

abstract：:Englesberg, E. (University of Pittsburgh, Pittsburgh, Pa.), R L. Anderson, R. Weinberg, N. Lee, P. Hoffee, G. Huttenhauer, and H. Boyer. l-Arabinose-sensitive, l-ribulose 5-phosphate 4-epimerase-deficient mutants of Escherichia coli. J. Bacteriol. 84:137-146. 1962-l-Arabinose-negative mutants of Escherichia coli B/r, ...

journal_title：Journal of bacteriology

authors： ENGLESBERG E,ANDERSON RL,WEINBERG R,LEE N,HOFFEE P,HUTTENHAUER G,BOYER H

更新日期：1962-07-01 00:00:00

abstract：:A DNA probe from an internal fragment of the exotoxin A structural gene was used to study the effects of selected culture conditions on steady-state levels of exotoxin-specific mRNA in Pseudomonas aeruginosa. Cells grown under conditions of iron deprivation began to synthesize and excrete the exotoxin A polypeptide du...

journal_title：Journal of bacteriology

authors： Lory S

更新日期：1986-12-01 00:00:00

abstract：:Uninduced cultures of Saccharomyces cerevisiae exhibit high basal levels of allantoinase, allantoicase, and ureidoglycolate hydrolase, the enzymes responsible for degrading allantoin to urea. As a result, these activities increase only 4- to 8-fold upon induction, whereas the urea-degrading enzymes, urea carboxylase a...

journal_title：Journal of bacteriology

authors： Zacharski CA,Cooper TG

更新日期：1978-08-01 00:00:00

abstract：:The first alkaline phosphatase (APase) structural gene mutant of Bacillus subtilis 168 was constructed by using a clone identified by hybridization to a synthetic degenerative oligonucleotide. The design of the probe was based on the first 29 amino acids of the sequenced mature APase III protein, which had been isolat...

journal_title：Journal of bacteriology

authors： Bookstein C,Edwards CW,Kapp NV,Hulett FM

更新日期：1990-07-01 00:00:00

abstract：:Virgibacillus halodenitrificans 1806 is an endospore-forming halophilic bacterium isolated from salterns in Korea. Here, we report the draft genome sequence of V. halodenitrificans 1806, which may reveal the molecular basis of osmoadaptation and insights into carbon and anaerobic metabolism in moderate halophiles. ...

journal_title：Journal of bacteriology

authors： Lee SJ,Lee YJ,Jeong H,Lee SJ,Lee HS,Pan JG,Kim BC,Lee DW

更新日期：2012-11-01 00:00:00

abstract：:Agrobacterium radiobacter NCIB 11883 was grown in lactose-limited continuous culture at a dilution rate of 0.045/h. Washed cells transported [14C]lactose and [methyl-14C]beta-D-thiogalactoside, a nonmetabolisable analog of lactose, at similar rates and with similar affinities (Km for transport, less than 1 microM). Tr...

journal_title：Journal of bacteriology

authors： Greenwood JA,Cornish A,Jones CW

更新日期：1990-04-01 00:00:00

abstract：:Methanogenesis from the non-physiological C1 donors thioproline, thiazolidine, hexamethylenetetramine, formaldehyde (HCHO), and HOCH2-S-coenzyme M (CoM) was catalyzed by cell extracts of Methanobacterium thermoautotrophicum under a hydrogen atmosphere. Tetrahydromethanopterin (H4MPT) and HS-CoM were required in the re...

journal_title：Journal of bacteriology

authors： Escalante-Semerena JC,Wolfe RS

更新日期：1985-02-01 00:00:00

abstract：:The presence of acetate and pyruvate groups in Klebsiella capsular polysaccharides may be demonstrated and estimated quantitatively by running the proton magnetic resonance spectrum of the polysaccharide (as sodium salt) in deuterium oxide at 95 C. Such spectra also permit an assessment to be made of the number of alp...

journal_title：Journal of bacteriology

authors： Bebault GM,Choy YM,Dutton GG,Funnell N,Stephen AM,Yang MT

更新日期：1973-03-01 00:00:00

abstract：:The genes that encode the two subunits of Bacillus subtilis phenylalanyl-tRNA synthetase were cloned from alpha lambda library of chromosomal B. subtilis DNA by specific complementation of a thermosensitive Escherichia coli pheS mutation. Both genes (we named them pheS and pheT, analogous to the corresponding genes of...

journal_title：Journal of bacteriology

authors： Brakhage AA,Putzer H,Shazand K,Röschenthaler RJ,Grunberg-Manago M

更新日期：1989-02-01 00:00:00

abstract：:We report the first study of tRNA modification in psychrotolerant archaea, specifically in the archaeon Methanococcoides burtonii grown at 4 and 23 degrees C. For comparison, unfractionated tRNA from the archaeal hyperthermophile Stetteria hydrogenophila cultured at 93 degrees C was examined. Analysis of modified nucl...

journal_title：Journal of bacteriology

authors： Noon KR,Guymon R,Crain PF,McCloskey JA,Thomm M,Lim J,Cavicchioli R

更新日期：2003-09-01 00:00:00

abstract：:The galP1 promoter is responsible for galactose-dependent, glucose-sensitive transcription of the galactose utilization operon of Streptomyces coelicolor and Streptomyces lividans. We describe the characterization of mutations that were positioned directly upstream of the apparent transcription start site of galP1 and...

journal_title：Journal of bacteriology

authors： Mattern SG,Brawner ME,Westpheling J

更新日期：1993-03-01 00:00:00

abstract：:The Bacillus subtilis ferric uptake regulator (Fur) protein is the major sensor of cellular iron status. When iron is limiting for growth, derepression of the Fur regulon increases the cellular capacity for iron uptake and mobilizes an iron-sparing response mediated in large part by a small noncoding RNA named FsrA. F...

journal_title：Journal of bacteriology

authors： Smaldone GT,Revelles O,Gaballa A,Sauer U,Antelmann H,Helmann JD

更新日期：2012-05-01 00:00:00

abstract：:Derivatives of Pseudomonas sp. B13 which had acquired the capability to utilize 4-chloro- and 3,5-dichlorobenzoate as a consequence of the introduction of genes of the TOL plasmid of Pseudomonas putida mt-2 were studied. The utilization of these substrates, a property not shared by the parent strains, was shown to dep...

journal_title：Journal of bacteriology

authors： Reineke W,Knackmuss HJ

更新日期：1980-05-01 00:00:00

abstract：:The interaction of Streptococcus pyogenes (group A streptococcus [GAS]) with its human host requires several surface proteins. In this study, we isolated mutations in a gene required for the surface localization of protein F by transposon mutagenesis of the M6 strain JRS4. This gene (srtA) encodes a protein homologous...

journal_title：Journal of bacteriology

authors： Barnett TC,Scott JR

更新日期：2002-04-01 00:00:00

abstract：:The stable inheritance of the broad-host-range plasmid RK2 is due at least in part to functions within a region located at coordinates 32.8 to 35.9 kb, termed the RK2 par locus. This locus encodes four previously identified genes in two operons (parCBA and parD; M. Gerlitz, O. Hrabak, and H. Schwab, J. Bacteriol. 172:...

journal_title：Journal of bacteriology

authors： Roberts RC,Helinski DR

更新日期：1992-12-01 00:00:00

abstract：:The reversion behavior of pleiotropic carbohydrate mutants, previously designated as ctr, was studied. The mutants revert to complete restoration of the wild-type phenotype, as well as to a spectrum of partial wild-type phenotypes. Lac(+) reversions were found in the lac region (11 min) and some Mal(+) reversions occu...

journal_title：Journal of bacteriology

authors： Wang RJ,Morse HG,Morse ML

更新日期：1970-12-01 00:00:00

abstract：:Conditions for continuous culture of Escherichia coli K-12 His- Thi- under glucose limitation were established. Both the capacity for respiration, at D greater than 0.2/h, and specific activity of superoxide dismutase increased as a function of specific growth rate, whereas peroxidase and catalase were either invarian...

journal_title：Journal of bacteriology

authors： Hassan HM,Fridovich I

更新日期：1977-05-01 00:00:00

abstract：:Cyclic 2,3-diphosphoglycerate synthetase (cDPGS) catalyzes the synthesis of cyclic 2,3-diphosphoglycerate (cDPG) by formation of an intramolecular phosphoanhydride bond in 2,3-diphosphoglycerate. cDPG is known to be accumulated to high intracellular concentrations (>300 mM) as a putative thermoadapter in some hyperthe...

journal_title：Journal of bacteriology

authors： Matussek K,Moritz P,Brunner N,Eckerskorn C,Hensel R

更新日期：1998-11-01 00:00:00

abstract：:The crystal structure of the Na(+)-coupled melibiose permease of Salmonella enterica serovar Typhimurium (MelBSt) demonstrates that MelB is a member of the major facilitator superfamily of transporters. Arg residues at positions 295, 141, and 363 are involved in interdomain interactions at the cytoplasmic side by gove...

journal_title：Journal of bacteriology

authors： Amin A,Ethayathulla AS,Guan L

更新日期：2014-09-01 00:00:00

abstract：:Periplasmic membrane fusion proteins (MFPs) are essential components of multidrug efflux pumps and type I protein secretion systems of gram-negative bacteria. Located in the periplasm, MFPs function by creating a physical link between inner membrane transporters and outer membrane channels. The most conserved sequence...

journal_title：Journal of bacteriology

authors： Ge Q,Yamada Y,Zgurskaya H

更新日期：2009-07-01 00:00:00

abstract：:The pattern of peptidoglycan (murein) segregation in cells of Escherichia coli with impaired activity of the morphogenetic proteins penicillin-binding protein 2 and RodA has been investigated by the D-cysteine-biotin immunolabeling technique (M. A. de Pedro, J. C. Quintela, J.-V. Höltje, and H. Schwarz, J. Bacteriol. ...

journal_title：Journal of bacteriology

authors： de Pedro MA,Donachie WD,Höltje JV,Schwarz H

更新日期：2001-07-01 00:00:00

abstract：:The current work by Jain et al. (S. Jain, A. M. Chang, M. Singh, J. S. McLean, et al., J Bacteriol 201:e00683-18, 2019, https://doi.org/10.1128/JB.00683-18) reports the cloning of the lipid A deacylase gene of Porphyromonas gingivalis and the phenotypic characterization of the enzyme. Attempts to clone the gene and th...

journal_title：Journal of bacteriology

authors： Progulske-Fox A

更新日期：2019-05-08 00:00:00

abstract：:The mecA-27r gene from Staphylococcus aureus 27r encodes penicillin-binding protein 2a (PBP2a-27r), which causes this strain to be methicillin resistant. Removal or replacement of the N-terminal transmembrane domain had no effect on binding of penicillin, but removal of portions of the putative transglycosylase domain...

journal_title：Journal of bacteriology

authors： Wu CY,Alborn WE Jr,Flokowitsch JE,Hoskins J,Unal S,Blaszczak LC,Preston DA,Skatrud PL

更新日期：1994-01-01 00:00:00

abstract：:Mycobacterium tuberculosis utilizes the methylerythritol phosphate (MEP) pathway for biosynthesis of isopentenyl diphosphate and its isomer, dimethylallyl diphosphate, precursors of all isoprenoid compounds. This pathway is of interest as a source of new drug targets, as it is absent from humans and disruption of the ...

journal_title：Journal of bacteriology

authors： Eoh H,Brown AC,Buetow L,Hunter WN,Parish T,Kaur D,Brennan PJ,Crick DC

更新日期：2007-12-01 00:00:00

abstract：:Rickettsia japonica strain YH, isolated in 1984 in Japan, is the type strain of R. japonica, the tick-borne agent of Japanese spotted fever. Here, we report the 1.33-Mb genome of this rickettsial species. ...

journal_title：Journal of bacteriology

authors： Dong X,El Karkouri K,Robert C,Raoult D,Fournier PE

更新日期：2012-12-01 00:00:00

abstract：:A gene (aad) coding for an aldehyde/alcohol dehydrogenase (AAD) was identified immediately upstream of the previously cloned ctfA (J. W. Cary, D. J. Petersen, E. T. Papoutsakis, and G. N. Bennett, Appl. Environ. Microbiol. 56:1576-1583, 1990) of Clostridium acetobutylicum ATCC 824 and sequenced. The 2,619-bp aad codes...

journal_title：Journal of bacteriology

authors： Nair RV,Bennett GN,Papoutsakis ET

更新日期：1994-02-01 00:00:00