The C-terminal domain of AcrA is essential for the assembly and function of the multidrug efflux pump AcrAB-TolC.

Abstract:

:Periplasmic membrane fusion proteins (MFPs) are essential components of multidrug efflux pumps and type I protein secretion systems of gram-negative bacteria. Located in the periplasm, MFPs function by creating a physical link between inner membrane transporters and outer membrane channels. The most conserved sequence of MFPs is located in their distal C-terminal domain. However, neither the structure nor the function of this domain is known. In this study, we investigated the structural and functional role of the C-terminal domain of Escherichia coli AcrA, a periplasmic component of the multidrug efflux pump AcrAB-TolC. Using trypsin proteolysis, we identified the proteolytically labile sites in the C-terminal domain (amino acid residues 315 to 397) of AcrA in vitro. We next used these sites as a map to evaluate the structural integrity of this domain of AcrA inside the periplasm. We found that the C-terminal domain of AcrA is protected from trypsin when the tripartite efflux pump AcrAB-TolC is assembled. In contrast, this domain remains proteolytically labile in cells producing only one of the AcrB or TolC components of the complex. Site-directed mutagenesis of 12 highly conserved amino acid residues of the C-terminal domain of AcrA showed that a single G363C substitution dramatically impairs the multidrug efflux activity of AcrAB-TolC. The G363C mutant interacts with both AcrB and TolC but fails to properly assemble into a functional complex. We conclude that the C-terminal domain of AcrA plays an important role in the assembly and function of AcrAB-TolC efflux pump.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Ge Q,Yamada Y,Zgurskaya H

doi

10.1128/JB.00204-09

subject

Has Abstract

pub_date

2009-07-01 00:00:00

pages

4365-71

issue

13

eissn

0021-9193

issn

1098-5530

pii

JB.00204-09

journal_volume

191

pub_type

杂志文章
  • Osmotic remedial response in a galactose-negative mutant of Saccharomyces cerevisiae.

    abstract::We investigated an osmotic remedial mutant of Saccharomyces which was deficient in galactose-1-phosphate uridyl transferase. Both the rate of growth and the transferase activity of the mutant culture were dependent on the osmotic activity of the growth medium. Organic and ionic solutes proved to be equally effective i...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.95.3.1103-1110.1968

    authors: Bassel J,Douglas HC

    更新日期:1968-03-01 00:00:00

  • Use of an inducible regulatory protein to identify members of a regulon: application to the regulon controlled by the leucine-responsive regulatory protein (Lrp) in Escherichia coli.

    abstract::Procedures were developed to facilitate the identification of genes that belong to a given regulon and characterization of their responses to the regulator. The regulon controlled by the Escherichia coli leucine-responsive regulatory protein (Lrp) was studied by isolating random transcriptional fusions to lacZ, using ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.20.6254-6263.1997

    authors: Bhagwat SP,Rice MR,Matthews RG,Blumenthal RM

    更新日期:1997-10-01 00:00:00

  • Bordetella pertussis major outer membrane porin protein forms small, anion-selective channels in lipid bilayer membranes.

    abstract::The major outer membrane protein of molecular weight 40,000 (the 40K protein) of a virulent isolate of Bordetella pertussis was purified to apparent homogeneity. The purified protein formed an oligomer band (of apparent molecular weight 90,000) on sodium dodecyl sulfate-polyacrylamide gels after solubilization at low ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.166.1.212-216.1986

    authors: Armstrong SK,Parr TR Jr,Parker CD,Hancock RE

    更新日期:1986-04-01 00:00:00

  • Characterization of Pseudomonas aeruginosa derepressed R-plasmids.

    abstract::A genetic study of conjugal transmissibility of two R-plasmids was undertaken in Pseudomonas aeruginosa. Conjugally derepressed mutants of the R-plasmids were isolated, and examination of 11 independent mutants revealed that 10 were recessive to the wild-type transfer repressor, whereas 1 mutant was cis dominant. Cros...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.130.2.596-603.1977

    authors: Chandler PM,Krishnapillai V

    更新日期:1977-05-01 00:00:00

  • Regulation of the cadA cadmium resistance determinant of Staphylococcus aureus plasmid pI258.

    abstract::Regulation of the cadA cadmium and zinc resistance determinant of Staphylococcus aureus plasmid pI258 was demonstrated by using gene fusions and direct measurements of transcription. In growth experiments, cells harboring the intact cadA operon were induced with different cations and challenged by an inhibitory concen...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.23.7643-7649.1991

    authors: Yoon KP,Misra TK,Silver S

    更新日期:1991-12-01 00:00:00

  • Assimilatory sulfate reduction in an Escherichia coli mutant lacking thioredoxin activity.

    abstract::An investigation of sulfate reduction in B tsnC*7004, a mutant of Escherichia coli lacking thioredoxin, is reported. Although thioredoxin is indispensable for the adenosine 3'-phosphate 5'-phosphosulfate (PAPS) sulfotransferase reaction under the usual conditions of assay in extracts of wild-type cells, the mutant gre...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.134.1.131-138.1978

    authors: Tsang ML,Schiff JA

    更新日期:1978-04-01 00:00:00

  • Surface protein composition of Aeromonas hydrophila strains virulent for fish: identification of a surface array protein.

    abstract::The surface protein composition of members of a serogroup of Aeromonas hydrophila which exhibit high virulence for fish was examined. Treatment of whole cells of representative strain A. hydrophila TF7 with 0.2 M glycine buffer (pH 4.0) resulted in the release of sheets of a tetragonal surface protein array. Sodium do...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.170.2.499-506.1988

    authors: Dooley JS,Trust TJ

    更新日期:1988-02-01 00:00:00

  • Characterization of enzymes of the branched-chain amino acid biosynthetic pathway in Methanococcus spp.

    abstract::Methanococcus aeolicus, Methanococcus maripaludis, and Methanococcus voltae contain similar levels of four enzymes of branched-chain amino acid biosynthesis: acetohydroxy acid synthase, acetohydroxy acid isomeroreductase, dihydroxy acid dehydratase, and transaminase B. Following growth at low partial pressures of H2-C...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.6.2086-2092.1991

    authors: Xing RY,Whitman WB

    更新日期:1991-03-01 00:00:00

  • Transcription of the ileS operon in the archaeon Methanobacterium thermoautotrophicum Marburg.

    abstract::In the thermophilic archaeon Methanobacterium thermoautotrophicum Marburg, the structural gene for isoleucyl-tRNA synthetase (ileS) is flanked upstream by orf401 and downstream by purL. orf401 encodes a 43.5-kDa protein with an unknown function. Northern (RNA) hybridization and S1 nuclease protection experiments showe...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.18.5945-5952.1993

    authors: Jenal U,Thurner C,Leisinger T

    更新日期:1993-09-01 00:00:00

  • Iron respiration-driven proton translocation in aerobic bacteria.

    abstract::Washed cell suspensions of Aquaspirillum magnetotacticum MS-1, A. itersonii E12639, Bacillus subtilis 6633, and Escherichia coli CSH27 translocated protons in response to the added oxidant O2 or NO3-, with triphenylmethylphosphonium bromide as the permeant ion. Iron respiration-driven proton translocation was observed...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.167.2.729-731.1986

    authors: Short KA,Blakemore RP

    更新日期:1986-08-01 00:00:00

  • New nalidixic acid resistance mutations related to deoxyribonucleic acid gyrase activity.

    abstract::In Escherichia coli K-12 mutants which had a new nalidixic acid resistance mutation at about 82 min on the chromosome map, cell growth was resistant to or hypersusceptible to nalidixic acid, oxolinic acid, piromidic acid, pipemidic acid, and novobiocin. Deoxyribonucleic acid gyrase activity as tested by supercoiling o...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.148.2.450-458.1981

    authors: Yamagishi J,Furutani Y,Inoue S,Ohue T,Nakamura S,Shimizu M

    更新日期:1981-11-01 00:00:00

  • Binding of Spo0A stimulates spoIIG promoter activity in Bacillus subtilis.

    abstract::The spoIIG promoter is used by RNA polymerase containing sigma A (E sigma A), the primary form of RNA polymerase found in vegetative cells in Bacillus subtilis. However, the spoIIG promoter is active only after the onset of sporulation. Activation of the spoIIG promoter requires the product of the spo0A gene (Spo0A). ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.5.1448-1453.1992

    authors: Satola SW,Baldus JM,Moran CP Jr

    更新日期:1992-03-01 00:00:00

  • The legacy of HfrH: mutations in the two-component system CreBC are responsible for the unusual phenotype of an Escherichia coli arcA mutant.

    abstract::Strains derived from HfrH carrying the arcA2 null mutation exhibit a higher respiratory rate, enhanced glucose consumption, and a more-reduced intracellular redox state than arcA deletion mutants of a different lineage. The phenotype of the arcA2 mutants was due to the presence of a creC constitutive mutation introduc...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00040-08

    authors: Nikel PI,de Almeida A,Pettinari MJ,Méndez BS

    更新日期:2008-05-01 00:00:00

  • Type 1 fimbriae contribute to catheter-associated urinary tract infections caused by Escherichia coli.

    abstract::Biofilm formation on catheters is thought to contribute to persistence of catheter-associated urinary tract infections (CAUTI), which represent the most frequent nosocomial infections. Knowledge of genetic factors for catheter colonization is limited, since their role has not been assessed using physicochemical condit...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00985-13

    authors: Reisner A,Maierl M,Jörger M,Krause R,Berger D,Haid A,Tesic D,Zechner EL

    更新日期:2014-03-01 00:00:00

  • CHROMOSOMAL LOCATION OF THYMINE AND ARGININE GENES IN ESCHERICHIA COLI AND AN F' INCORPORATING THEM.

    abstract::Ishibashi, Masahide (Osaka University, Osaka, Japan), Yoshinobu Sugino, and Yukinori Hirota. Chromosomal location of thymine and arginine genes in Escherichia coli and an F' incorporating them. J. Bacteriol. 87:554-561. 1964.-The gene responsible for thymine requirement or independence in Escherichia coli K-12 is loca...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.87.3.554-561.1964

    authors: ISHIBASHI M,SUGINO Y,HIROTA Y

    更新日期:1964-03-01 00:00:00

  • Annotated genome sequence of Lactobacillus pentosus MP-10, which has probiotic potential, from naturally fermented Aloreña green table olives.

    abstract::Lactobacillus pentosus MP-10 was isolated from brines of naturally fermented Aloreña green table olives. MP-10 has potential probiotic traits, including inhibition of human pathogenic bacteria, survival at low pH (1.5), and bile salt tolerance (3%). Here, we report for the first time the annotated genome sequence of L...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.05171-11

    authors: Abriouel H,Benomar N,Pulido RP,Cañamero MM,Gálvez A

    更新日期:2011-09-01 00:00:00

  • Translation of trpG in Bacillus subtilis is regulated by the trp RNA-binding attenuation protein (TRAP).

    abstract::trpG of Bacillus subtilis encodes a glutamine amidotransferase subunit that is involved in the synthesis of both folic acid and L-tryptophan. Expression of trpG is negatively regulated by tryptophan even though this gene is located within a folic acid biosynthetic operon. Examination of both transcriptional and transl...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.15.4272-4278.1995

    authors: Yang M,de Saizieu A,van Loon AP,Gollnick P

    更新日期:1995-08-01 00:00:00

  • Dual negative control of spx transcription initiation from the P3 promoter by repressors PerR and YodB in Bacillus subtilis.

    abstract::The spx gene encodes an RNA polymerase-binding protein that exerts negative and positive transcriptional control in response to oxidative stress in Bacillus subtilis. It resides in the yjbC-spx operon and is transcribed from at least five promoters located in the yjbC regulatory region or in the yjbC-spx intergenic re...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01520-06

    authors: Leelakriangsak M,Kobayashi K,Zuber P

    更新日期:2007-03-01 00:00:00

  • Molecular cloning of structural and immunity genes for megacins A-216 and A-19213 in Bacillus megaterium.

    abstract::A host-vector system was developed for molecular cloning in Bacillus megaterium and used to clone the structural and immunity genes for megacins A-216 and A-19213. Recombinant clones that expressed immunity only or both immunity to and production of each megacin were obtained. Restriction mapping of native megacinogen...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.160.3.854-859.1984

    authors: Von Tersch MA,Carlton BC

    更新日期:1984-12-01 00:00:00

  • SUPPRESSION OF ROUS SARCOMA VIRUS GROWTH IN TISSUE CULTURES BY MYCOPLASMA ORALE.

    abstract::Somerson, Norman L. (National Institute of Allergy and Infectious Diseases, Bethesda, Md.), and M. K. Cook. Suppression of Rous sarcoma virus growth in tissue cultures by Mycoplasma orale. J. Bacteriol. 90:534-540. 1965.-An agent which produced cell destruction in human diploid and chick-embryo fibroblasts was isolate...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.90.2.534-540.1965

    authors: SOMERSON NL,COOK MK

    更新日期:1965-08-01 00:00:00

  • Magnesium starvation of Aerobacter aerogenes. II. Rates of nucleic acid synthesis and methods for their measurement.

    abstract::The rates of synthesis of Aerobacter aerogenes nucleic acids were estimated during incubation of the bacteria in a Mg(++)-free medium. Deoxyribonucleic acid (DNA) synthesized during Mg(++) starvation, or in the preceding exponential growth, remained acid-precipitable for 2.5 hr before breaking down to acid-soluble pro...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.93.1.345-356.1967

    authors: Kennell D,Kotoulas A

    更新日期:1967-01-01 00:00:00

  • A study of iterative type II polyketide synthases, using bacterial genes cloned from soil DNA: a means to access and use genes from uncultured microorganisms.

    abstract::To examine as randomly as possible the role of the beta-ketoacyl and acyl carrier protein (ACP) components of bacterial type II polyketide synthases (PKSs), homologs of the chain-length-factor (CLF) genes were cloned from the environmental community of microorganisms. With PCR primers derived from conserved regions of...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.23.7360-7368.1997

    authors: Seow KT,Meurer G,Gerlitz M,Wendt-Pienkowski E,Hutchinson CR,Davies J

    更新日期:1997-12-01 00:00:00

  • Immunochemistry of the Group-Specific Polysaccharide of Nocardia brasiliensis.

    abstract::Estrada-Parra, Sergio (Escuela Nacional de Ciencias Biológicas, México, D.F., México), Abel Zamora, and L. F. Bojalil. Immunochemistry of the group-specific polysaccharide of Nocardia brasiliensis. J. Bacteriol. 90:571-574. 1965.-The group-specific polysaccharide of Nocardia brasiliensis was further purified, yielding...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.90.3.571-574.1965

    authors: Estrada-Parra S,Zamora A,Bojalil LF

    更新日期:1965-09-01 00:00:00

  • Overproduction of the rbo gene product from Desulfovibrio species suppresses all deleterious effects of lack of superoxide dismutase in Escherichia coli.

    abstract::In an attempt to isolate the superoxide dismutase (SOD) gene from the anaerobic sulfate-reducing bacterium Desulfoarculus baarsii, a DNA fragment was isolated which functionally complemented an Escherichia coli mutant (sodA sodB) deficient in cytoplasmic SODs. This region carries two open reading frames with sequences...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.23.6736-6742.1996

    authors: Pianzzola MJ,Soubes M,Touati D

    更新日期:1996-12-01 00:00:00

  • Ultraviolet- and X-ray-induced responses of a deoxyribonucleic acid polymerase-deficient mutant of Escherichia coli.

    abstract::Escherichia coli K-12, polAl(-) is a mutant strain whose extracts are deficient in Kornberg deoxyribonucleic acid (DNA) polymerase activity. We have compared the mutant and parental strains on the basis of a number of responses to ultraviolet (UV) and X-irradiation. For both types of radiation, the mutant is more sens...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.107.1.61-67.1971

    authors: Paterson MC,Boyle JM,Setlow RB

    更新日期:1971-07-01 00:00:00

  • Interactions between CdsD, CdsQ, and CdsL, three putative Chlamydophila pneumoniae type III secretion proteins.

    abstract::Chlamydophila pneumoniae is a gram-negative obligate intracellular bacterial pathogen that causes pneumonia and bronchitis and may contribute to atherosclerosis. The developmental cycle of C. pneumoniae includes a morphological transition from an infectious extracellular elementary body (EB) to a noninfectious intrace...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01997-07

    authors: Johnson DL,Stone CB,Mahony JB

    更新日期:2008-04-01 00:00:00

  • Functional genomics enables identification of genes of the arginine transaminase pathway in Pseudomonas aeruginosa.

    abstract::Arginine utilization in Pseudomonas aeruginosa with multiple catabolic pathways represents one of the best examples of the metabolic versatility of this organism. To identify genes involved in arginine catabolism, we have employed DNA microarrays to analyze the transcriptional profiles of this organism in response to ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00261-07

    authors: Yang Z,Lu CD

    更新日期:2007-06-01 00:00:00

  • Membrane topology and DNA-binding ability of the Streptococcal CpsA protein.

    abstract::Many streptococcal pathogens require a polysaccharide capsule for survival in the host during systemic infection. The highly conserved CpsA protein is proposed to be a transcriptional regulator of capsule production in streptococci, although the regulatory mechanism is unknown. Hydropathy plots of CpsA predict an inte...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01098-10

    authors: Hanson BR,Lowe BA,Neely MN

    更新日期:2011-01-01 00:00:00

  • Directed evolution of toluene ortho-monooxygenase for enhanced 1-naphthol synthesis and chlorinated ethene degradation.

    abstract::Trichloroethylene (TCE) is the most frequently detected groundwater contaminant, and 1-naphthol is an important chemical manufacturing intermediate. Directed evolution was used to increase the activity of toluene ortho-monooxygenase (TOM) of Burkholderia cepacia G4 for both chlorinated ethenes and naphthalene oxidatio...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.184.2.344-349.2002

    authors: Canada KA,Iwashita S,Shim H,Wood TK

    更新日期:2002-01-01 00:00:00

  • Genome sequence of Reyranella massiliensis, a bacterium associated with amoebae.

    abstract::Reyranella massiliensis is an Alphaproteobacterium member of the class Rhodospirillaceae, growing in amoebae. We sequenced the genome of type strain 521(T). It is composed of a 5,792,218-bp chromosome and encodes 5,675 protein-coding genes and 53 RNA genes, including 3 rRNA genes. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01260-12

    authors: Pagnier I,Croce O,Robert C,Raoult D,La Scola B

    更新日期:2012-10-01 00:00:00