Type 1 fimbriae contribute to catheter-associated urinary tract infections caused by Escherichia coli.

Abstract:

:Biofilm formation on catheters is thought to contribute to persistence of catheter-associated urinary tract infections (CAUTI), which represent the most frequent nosocomial infections. Knowledge of genetic factors for catheter colonization is limited, since their role has not been assessed using physicochemical conditions prevailing in a catheterized human bladder. The current study aimed to combine data from a dynamic catheterized bladder model in vitro with in vivo expression analysis for understanding molecular factors relevant for CAUTI caused by Escherichia coli. By application of the in vitro model that mirrors the physicochemical environment during human infection, we found that an E. coli K-12 mutant defective in type 1 fimbriae, but not isogenic mutants lacking flagella or antigen 43, was outcompeted by the wild-type strain during prolonged catheter colonization. The importance of type 1 fimbriae for catheter colonization was verified using a fimA mutant of uropathogenic E. coli strain CFT073 with human and artificial urine. Orientation of the invertible element (IE) controlling type 1 fimbrial expression in bacterial populations harvested from the colonized catheterized bladder in vitro suggested that the vast majority of catheter-colonizing cells (up to 88%) express type 1 fimbriae. Analysis of IE orientation in E. coli populations harvested from patient catheters revealed that a median level of ∼73% of cells from nine samples have switched on type 1 fimbrial expression. This study supports the utility of the dynamic catheterized bladder model for analyzing catheter colonization factors and highlights a role for type 1 fimbriae during CAUTI.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Reisner A,Maierl M,Jörger M,Krause R,Berger D,Haid A,Tesic D,Zechner EL

doi

10.1128/JB.00985-13

subject

Has Abstract

pub_date

2014-03-01 00:00:00

pages

931-9

issue

5

eissn

0021-9193

issn

1098-5530

pii

JB.00985-13

journal_volume

196

pub_type

杂志文章
  • Genetic determinants of Silicibacter sp. TM1040 motility.

    abstract::Silicibacter sp. TM1040 is a member of the marine Roseobacter clade of Alphaproteobacteria that forms symbioses with unicellular eukaryotic phytoplankton, such as dinoflagellates. The symbiosis is complex and involves a series of steps that physiologically change highly motile bacteria into cells that readily form bio...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00429-09

    authors: Belas R,Horikawa E,Aizawa S,Suvanasuthi R

    更新日期:2009-07-01 00:00:00

  • The Rnf Complex Is an Energy-Coupled Transhydrogenase Essential To Reversibly Link Cellular NADH and Ferredoxin Pools in the Acetogen Acetobacterium woodii.

    abstract::The Rnf complex is a respiratory enzyme that catalyzes the oxidation of reduced ferredoxin to the reduction of NAD+, and the negative free energy change of this reaction is used to generate a transmembrane ion gradient. In one class of anaerobic acetogenic bacteria, the Rnf complex is believed to be essential for ener...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00357-18

    authors: Westphal L,Wiechmann A,Baker J,Minton NP,Müller V

    更新日期:2018-10-10 00:00:00

  • Effect of glutamine on enzymes of nitrogen metabolism in Bacillus subtilis.

    abstract::An earlier study of the regulation of glutamate synthase (GOGAT) in Bacillus subtilis (Deshpande et al., Bichem. Biophys. Res. Commun. 95:55--60, 1980) revealed an inverse relationship between the specific activity of this essential ammonia-assimilatory enzyme and the intracellular pool of glutamine: GOGAT activity de...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.145.2.768-774.1981

    authors: Deshpande KL,Katze JR,Kane JF

    更新日期:1981-02-01 00:00:00

  • lpt6, a gene required for addition of phosphoethanolamine to inner-core lipopolysaccharide of Neisseria meningitidis and Haemophilus influenzae.

    abstract::We previously described a gene, lpt3, required for the addition of phosphoethanolamine (PEtn) at the 3 position on the beta-chain heptose (HepII) of the inner-core Neisseria meningitidis lipopolysaccharide (LPS), but it has long been recognized that the inner-core LPS of some strains possesses PEtn at the 6 position (...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.186.20.6970-6982.2004

    authors: Wright JC,Hood DW,Randle GA,Makepeace K,Cox AD,Li J,Chalmers R,Richards JC,Moxon ER

    更新日期:2004-10-01 00:00:00

  • Protoplast formation in Escherichia coli.

    abstract::A procedure for protoplast formation in Escherichia coli is described. Removal of the cell wall was confirmed by examination of cells in thin-section preparations. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.128.2.668-670.1976

    authors: Weiss RL

    更新日期:1976-11-01 00:00:00

  • A novel testosterone catabolic pathway in bacteria.

    abstract::Forty years ago, Coulter and Talalay (A. W. Coulter and P. Talalay, J. Biol. Chem. 243:3238-3247, 1968) established the oxygenase-dependent pathway for the degradation of testosterone by aerobes. The oxic testosterone catabolic pathway involves several oxygen-dependent reactions and is not available for anaerobes. Sin...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00331-11

    authors: Leu YL,Wang PH,Shiao MS,Ismail W,Chiang YR

    更新日期:2011-09-01 00:00:00

  • Dissimilatory reduction of bisulfite by Desulfovibrio vulgaris.

    abstract::The reduction of bisulfite by Desulfovibrio vulgaris was investigated. Crude extracts reduced bisulfite to sulfide without the formation (detection) of any intermediates such as trithionate or thiosulfate. When the particulate fractions was removed from crude extracts by high-speed centrifugation, the soluble supernat...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.136.3.916-923.1978

    authors: Drake HL,Akagi JM

    更新日期:1978-12-01 00:00:00

  • Functional relationship among the gene clusters encoding F7(1), F7(2), F9, and F11 fimbriae of human uropathogenic Escherichia coli.

    abstract::The P fimbrial gene clusters encoding the serologically different F7(1), F7(2), F9, and F11 fimbriae were compared functionally. The results show that these gene clusters are closely related. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.167.1.407-410.1986

    authors: Van Die I,Van Megen I,Zuidweg E,Hoekstra W,De Ree H,Van den Bosch H,Bergmans H

    更新日期:1986-07-01 00:00:00

  • Characterization of late acyltransferase genes of Yersinia pestis and their role in temperature-dependent lipid A variation.

    abstract::Yersinia pestis is an important human pathogen that is maintained in flea-rodent enzootic cycles in many parts of the world. During its life cycle, Y. pestis senses host-specific environmental cues such as temperature and regulates gene expression appropriately to adapt to the insect or mammalian host. For example, Y....

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.188.4.1381-1388.2006

    authors: Rebeil R,Ernst RK,Jarrett CO,Adams KN,Miller SI,Hinnebusch BJ

    更新日期:2006-02-01 00:00:00

  • DifA, a methyl-accepting chemoreceptor protein-like sensory protein, uses a novel signaling mechanism to regulate exopolysaccharide production in Myxococcus xanthus.

    abstract::DifA is a methyl-accepting chemotaxis protein (MCP)-like sensory transducer that regulates exopolysaccharide (EPS) production in Myxococcus xanthus. Here mutational analysis and molecular biology were used to probe the signaling mechanisms of DifA in EPS regulation. We first identified the start codon of DifA experime...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00944-10

    authors: Xu Q,Black WP,Nascimi HM,Yang Z

    更新日期:2011-02-01 00:00:00

  • The SPOR Domain, a Widely Conserved Peptidoglycan Binding Domain That Targets Proteins to the Site of Cell Division.

    abstract::Sporulation-related repeat (SPOR) domains are small peptidoglycan (PG) binding domains found in thousands of bacterial proteins. The name "SPOR domain" stems from the fact that several early examples came from proteins involved in sporulation, but SPOR domain proteins are quite diverse and contribute to a variety of p...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章,评审

    doi:10.1128/JB.00118-17

    authors: Yahashiri A,Jorgenson MA,Weiss DS

    更新日期:2017-06-27 00:00:00

  • Subcellular localization of a small sporulation protein in Bacillus subtilis.

    abstract::SpoVM is an unusually small (26-residue-long) protein that is produced in the mother cell chamber of the sporangium during the process of sporulation in Bacillus subtilis. We investigated the subcellular localization of SpoVM, which is believed to be an amphipathic alpha-helix, by using a fusion of the sporulation pro...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.185.4.1391-1398.2003

    authors: van Ooij C,Losick R

    更新日期:2003-02-01 00:00:00

  • Role of CodY in regulation of the Bacillus subtilis hut operon.

    abstract::Bacillus subtilis mutants deficient in amino acid repression of the histidine utilization (hut) operon were isolated by transposon mutagenesis. Genetic characterization of these mutants indicated that they most likely contained transposon insertions within the codVWXY operon. The codY gene is required for nutritional ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.13.3779-3784.1996

    authors: Fisher SH,Rohrer K,Ferson AE

    更新日期:1996-07-01 00:00:00

  • The Bacillus subtilis dacB gene, encoding penicillin-binding protein 5*, is part of a three-gene operon required for proper spore cortex synthesis and spore core dehydration.

    abstract::Studies of gene expression using fusions to lacZ demonstrated that the Bacillus subtilis dacB gene, encoding penicillin-binding protein 5*, is in an operon with two downstream genes, spmA and spmB. Mutations affecting any one of these three genes resulted in the production of spores with reduced heat resistance. The c...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.16.4721-4729.1995

    authors: Popham DL,Illades-Aguiar B,Setlow P

    更新日期:1995-08-01 00:00:00

  • Antipain lethality to Escherichia coli: dependence upon cyclic adenosine 3',5'-monophosphate and its receptor protein.

    abstract::Antipain kills Escherichia coli K-12 cells in an exponential manner beginning 1 h after its addition. Mutant strains, delta cya and crp, which are unable to synthesize cyclic adenosine 3',5'-monophosphate (cAMP) and the cAMP receptor protein, respectively, are not affected. Addition of cAMP (5 mM) to antipain-treated ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.139.2.690-693.1979

    authors: Swenson PA

    更新日期:1979-08-01 00:00:00

  • Cell walls and lysis of Mortierella parvispora hyphae.

    abstract::Walls of Mortierella parvispora, Pullularia pullulans, Absidia repens, Fusarium oxysporum, and of several Penicillium species varied in their susceptibilities to digestion by glucanase and chitinase. Polysaccharides were present in the residues remaining after enzymatic digestion. Acid hydrolysates of the walls contai...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.97.3.1056-1061.1969

    authors: Pengra RM,Cole MA,Alexander M

    更新日期:1969-03-01 00:00:00

  • DIFFERENTIATION OF PATHOGENIC AND SAPROPHYTIC LEPTOSPIRES WITH 8-AZAGUANINE.

    abstract::Johnson, Russell C. (University of Minnesota, Minneapolis), and Palmer Rogers. Differentiation of pathogenic and saprophytic leptospires with 8-azaguanine. J. Bacteriol. 88:1618-1623. 1964.-The use of the purine analogue, 8-azaguanine, as a differential agent for the separation of pathogenic and saprophytic leptospire...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.88.6.1618-1623.1964

    authors: JOHNSON RC,ROGERS P

    更新日期:1964-12-01 00:00:00

  • Unique lipids in Azotobacter vinelandii cysts: synthesis, distribution, and fate during germination.

    abstract::Unique lipids found in Azotobacter vinelandii cysts are derived primarily from beta-hydroxybutyrate used to induce encystment. Tracer studies with beta-[14C]hydroxybutyrate showed that the biosynthesis of these compounds during encystment began at 8 to 12 h after induction and reached maximal levels after 2 days, Seve...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.147.1.91-96.1981

    authors: Su CJ,Sadoff HL

    更新日期:1981-07-01 00:00:00

  • IF3-mediated suppression of a GUA initiation codon mutation in the recJ gene of Escherichia coli.

    abstract::A mutational change of the initiation codon to GUA was found to reduce, but not abolish, expression of the recJ gene of Escherichia coli. Specific mutations in translational initiation factor IF3 have been isolated as second-site suppressors of this GUA initiation codon mutation. One of these, infC135, with an arginin...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.21.6705-6713.1997

    authors: Haggerty TJ,Lovett ST

    更新日期:1997-11-01 00:00:00

  • Sugar transport through maltoporin of Escherichia coli: role of the greasy slide.

    abstract::The lining of the maltodextrin-specific maltoporin (LamB) channel exhibits a string of aromatic residues, the greasy slide, part of which has been shown previously by crystallography to be involved in substrate binding. To probe the functional role of the greasy slide, alanine scanning mutagenesis has been performed o...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.184.11.2994-2999.2002

    authors: Van Gelder P,Dumas F,Bartoldus I,Saint N,Prilipov A,Winterhalter M,Wang Y,Philippsen A,Rosenbusch JP,Schirmer T

    更新日期:2002-06-01 00:00:00

  • Isolation and preliminary characterization of auxotrophs of a filamentous Cyanobacterium.

    abstract::Auxotrophic mutants of the filamentous cyanobacterium Anabaena variabilis were isolated by a method in which, after mutagenesis and before penicllin enrichment, mutant and wild-type cells were separated by cavitation. Auxotrophs were identified by their inability to grow on minimal medium, and they were partially char...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.129.3.1556-1562.1977

    authors: Currier TC,Haury JF,Wolk CP

    更新日期:1977-03-01 00:00:00

  • Use of a mariner-based transposon mutagenesis system to isolate Clostridium perfringens mutants deficient in gliding motility.

    abstract::Clostridium perfringens is an anaerobic Gram-positive pathogen that causes many human and animal diseases, including food poisoning and gas gangrene. C. perfringens lacks flagella but possesses type IV pili (TFP). We have previously shown that C. perfringens can glide across an agar surface in long filaments composed ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01288-12

    authors: Liu H,Bouillaut L,Sonenshein AL,Melville SB

    更新日期:2013-02-01 00:00:00

  • Characterization of a novel thermostable carboxylesterase from Geobacillus kaustophilus HTA426 shows the existence of a new carboxylesterase family.

    abstract::The gene GK3045 (741 bp) from Geobacillus kaustophilus HTA426 was cloned, sequenced, and overexpressed into Escherichia coli Rosetta (DE3). The deduced protein was a 30-kDa monomeric esterase with high homology to carboxylesterases from Geobacillus thermoleovorans NY (99% identity) and Geobacillus stearothermophilus (...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01060-08

    authors: Montoro-García S,Martínez-Martínez I,Navarro-Fernández J,Takami H,García-Carmona F,Sánchez-Ferrer A

    更新日期:2009-05-01 00:00:00

  • Direction of deoxyribonucleic acid replication in Escherichia coli under various conditions of cell growth.

    abstract::The direction of chromosome replication in a temperature-sensitive initiation mutant of Escherichia coli (CT28) is shown autoradiographically to be bidirectional. This mode of replication persists even when the rate of replication is reduced by slow growth in succinate minimal medium or in the presence of chlorampheni...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.125.1.346-352.1976

    authors: Rodriguez RL,Davern CI

    更新日期:1976-01-01 00:00:00

  • MalFGK complex assembly and transport and regulatory characteristics of MalK insertion mutants.

    abstract::MalK is a peripheral cytoplasmic membrane protein that has multiple activities in Escherichia coli. It associates with integral cytoplasmic membrane proteins MalF and MalG to form the maltose transport complex (MalFGK), a member of the ATP-binding cassette (ABC) superfamily of proteins. In addition, MalK participates ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.4.1337-1343.1997

    authors: Lippincott J,Traxler B

    更新日期:1997-02-01 00:00:00

  • Proline-dependent regulation of Clostridium difficile Stickland metabolism.

    abstract::Clostridium difficile, a proteolytic Gram-positive anaerobe, has emerged as a significant nosocomial pathogen. Stickland fermentation reactions are thought to be important for growth of C. difficile and appear to influence toxin production. In Stickland reactions, pairs of amino acids donate and accept electrons, gene...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01492-12

    authors: Bouillaut L,Self WT,Sonenshein AL

    更新日期:2013-02-01 00:00:00

  • Synthesis and localization of the Salmonella SPI-1 type III secretion needle complex proteins PrgI and PrgJ.

    abstract::An essential component of type III secretion systems (TTSS) is a supramolecular structure termed the needle complex. In Salmonella enterica, at least four proteins make up this structure: InvG, PrgH, PrgK, and PrgI. Another protein, PrgJ, is thought to play a role in the assembly of this structure, but its function is...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.185.11.3480-3483.2003

    authors: Sukhan A,Kubori T,Galán JE

    更新日期:2003-06-01 00:00:00

  • Hairy root: plasmid encodes virulence traits in Agrobacterium rhizogenes.

    abstract::Agrobacterium rhizogenes strain 15834, which incites hairy root disease in plants, harbors three large plasmids: pAr15834a (107 x 10(6) daltons), pAr15834b (154 x 10(6) daltons), and pAr15834c (258 x 10(6) daltons). Kanamycin-resistant transconjugants were selected in a cross of kanamycin-resistant derivate of strain ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.141.3.1134-1141.1980

    authors: White FF,Nester EW

    更新日期:1980-03-01 00:00:00

  • DECREASED PERMEABILITY AS THE MECHANISM OF ARSENITE RESISTANCE IN PSEUDOMONAS PSEUDOMALLEI.

    abstract::Beppu, Michiko (University of Tokyo, Tokyo, Japan), and Kei Arima. Decreased permeability as the mechanism of arsenite resistance in Pseudomonas pseudomallei. J. Bacteriol. 88:151-157. 1964.-The mechanism of arsenite resistance of Pseudomonas pseudomallei strain 54, isolated from soil, was studied by use of radioactiv...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.88.1.151-157.1964

    authors: BEPPU M,ARIMA K

    更新日期:1964-07-01 00:00:00

  • Fine structure of Vibrio cholerae during toxin production.

    abstract::The fine structural changes associated with cell growth and toxin production have been examined in Vibrio cholerae strain 569B. No morphological alterations in the cell envelope are apparent during logarithmic growth with thin-section techniques. However, internal swelling, suggesting alteration of cell envelope perme...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.100.3.1393-1401.1969

    authors: Kennedy JR,Richardson SH

    更新日期:1969-12-01 00:00:00