Abstract:
:Biofilm formation on catheters is thought to contribute to persistence of catheter-associated urinary tract infections (CAUTI), which represent the most frequent nosocomial infections. Knowledge of genetic factors for catheter colonization is limited, since their role has not been assessed using physicochemical conditions prevailing in a catheterized human bladder. The current study aimed to combine data from a dynamic catheterized bladder model in vitro with in vivo expression analysis for understanding molecular factors relevant for CAUTI caused by Escherichia coli. By application of the in vitro model that mirrors the physicochemical environment during human infection, we found that an E. coli K-12 mutant defective in type 1 fimbriae, but not isogenic mutants lacking flagella or antigen 43, was outcompeted by the wild-type strain during prolonged catheter colonization. The importance of type 1 fimbriae for catheter colonization was verified using a fimA mutant of uropathogenic E. coli strain CFT073 with human and artificial urine. Orientation of the invertible element (IE) controlling type 1 fimbrial expression in bacterial populations harvested from the colonized catheterized bladder in vitro suggested that the vast majority of catheter-colonizing cells (up to 88%) express type 1 fimbriae. Analysis of IE orientation in E. coli populations harvested from patient catheters revealed that a median level of ∼73% of cells from nine samples have switched on type 1 fimbrial expression. This study supports the utility of the dynamic catheterized bladder model for analyzing catheter colonization factors and highlights a role for type 1 fimbriae during CAUTI.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Reisner A,Maierl M,Jörger M,Krause R,Berger D,Haid A,Tesic D,Zechner ELdoi
10.1128/JB.00985-13subject
Has Abstractpub_date
2014-03-01 00:00:00pages
931-9issue
5eissn
0021-9193issn
1098-5530pii
JB.00985-13journal_volume
196pub_type
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abstract::Forty years ago, Coulter and Talalay (A. W. Coulter and P. Talalay, J. Biol. Chem. 243:3238-3247, 1968) established the oxygenase-dependent pathway for the degradation of testosterone by aerobes. The oxic testosterone catabolic pathway involves several oxygen-dependent reactions and is not available for anaerobes. Sin...
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journal_title:Journal of bacteriology
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更新日期:1986-07-01 00:00:00
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更新日期:2003-02-01 00:00:00
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doi:10.1128/jb.178.13.3779-3784.1996
更新日期:1996-07-01 00:00:00
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journal_title:Journal of bacteriology
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doi:10.1128/jb.177.16.4721-4729.1995
更新日期:1995-08-01 00:00:00
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更新日期:1979-08-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JB.97.3.1056-1061.1969
更新日期:1969-03-01 00:00:00
abstract::Johnson, Russell C. (University of Minnesota, Minneapolis), and Palmer Rogers. Differentiation of pathogenic and saprophytic leptospires with 8-azaguanine. J. Bacteriol. 88:1618-1623. 1964.-The use of the purine analogue, 8-azaguanine, as a differential agent for the separation of pathogenic and saprophytic leptospire...
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更新日期:2013-02-01 00:00:00
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更新日期:1997-02-01 00:00:00
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journal_title:Journal of bacteriology
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更新日期:2013-02-01 00:00:00
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journal_title:Journal of bacteriology
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doi:10.1128/JB.141.3.1134-1141.1980
更新日期:1980-03-01 00:00:00
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更新日期:1964-07-01 00:00:00
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更新日期:1969-12-01 00:00:00