Abstract:
:Clostridium perfringens is an anaerobic Gram-positive pathogen that causes many human and animal diseases, including food poisoning and gas gangrene. C. perfringens lacks flagella but possesses type IV pili (TFP). We have previously shown that C. perfringens can glide across an agar surface in long filaments composed of individual bacteria attached end to end and that two TFP-associated proteins, PilT and PilC, are needed for this. To discover additional gene products that play a role in gliding, we developed a plasmid-based mariner transposon mutagenesis system that works effectively in C. perfringens. More than 10,000 clones were screened for mutants that lacked the ability to move away from the edge of a colony. Twenty-four mutants (0.24%) were identified that fit the criteria. The genes containing insertions that affected gliding motility fell into nine different categories. One gene, CPE0278, which encodes a homolog of the SagA cell wall-dependent endopeptidase, acquired distinct transposon insertions in two independent mutants. sagA mutants were unable to form filaments due to a complete lack of end-to-end connections essential for gliding motility. Complementation of the sagA mutants with a wild-type copy of the gene restored gliding motility. We constructed an in-frame deletion mutation in the sagA gene and found that this mutant had a phenotype similar to those of the transposon mutants. We hypothesize that the sagA mutant strains are unable to form the molecular complexes which are needed to keep the cells in an end-to-end orientation, leading to separation of daughter cells and the inability to carry out gliding motility.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Liu H,Bouillaut L,Sonenshein AL,Melville SBdoi
10.1128/JB.01288-12subject
Has Abstractpub_date
2013-02-01 00:00:00pages
629-36issue
3eissn
0021-9193issn
1098-5530pii
JB.01288-12journal_volume
195pub_type
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.6.2533-2536.1988
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journal_title:Journal of bacteriology
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doi:10.1128/JB.94.4.1016-1024.1967
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1992-11-01 00:00:00
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journal_title:Journal of bacteriology
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更新日期:1969-10-01 00:00:00
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更新日期:1987-02-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1983-06-01 00:00:00
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journal_title:Journal of bacteriology
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.166.3.818-823.1986
更新日期:1986-06-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2004-02-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.7.3541-3548.1990
更新日期:1990-07-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.118.3.880-889.1974
更新日期:1974-06-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1997-05-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2012-09-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.5.2835-2841.1989
更新日期:1989-05-01 00:00:00
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journal_title:Journal of bacteriology
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doi:10.1128/jb.172.4.1719-1724.1990
更新日期:1990-04-01 00:00:00
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journal_title:Journal of bacteriology
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doi:10.1128/JB.149.3.941-947.1982
更新日期:1982-03-01 00:00:00
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journal_title:Journal of bacteriology
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更新日期:1964-08-01 00:00:00
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更新日期:1993-05-01 00:00:00
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pub_type: 杂志文章
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更新日期:2003-02-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1981-03-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2004-09-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1998-07-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1984-06-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.6.2013-2024.2001
更新日期:2001-03-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.1.224-235.1990
更新日期:1990-01-01 00:00:00
abstract::Calcium-dependent exonuclease activity was produced by sporulating cells of Bacillus subtilis 168. Nuclease activity was released into the culture medium at approximately the same time as sporulation proteases, and production of these enzymes was tied to DNA replication. Results suggest that nuclease production is a f...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.149.2.768-770.1982
更新日期:1982-02-01 00:00:00