Hairy root: plasmid encodes virulence traits in Agrobacterium rhizogenes.

Abstract:

:Agrobacterium rhizogenes strain 15834, which incites hairy root disease in plants, harbors three large plasmids: pAr15834a (107 x 10(6) daltons), pAr15834b (154 x 10(6) daltons), and pAr15834c (258 x 10(6) daltons). Kanamycin-resistant transconjugants were selected in a cross of kanamycin-resistant derivate of strain 15834 and an avirulent recipient. The transconjugants belonging to one class were virulent and contained all three donor plasmids. These transconjugants also acquired sensitivity to the bacteriocin agrocin 84. The loss of plasmids from virulent transconjugants during growth at 37 degrees C indicated that virulence genes reside on pAr15834b, whereas agrocin 84 sensitivity genes reside on pAr15834a. The pathology induced by the virulent transconjugants containing only pAr15834b was identical to that produced by the wild-type strain of A. rhizogenes. Restriction endonuclease fragment analysis of plasmids from the transconjugants and the donor revealed that pAr15834c is a cointegrate of pAr15834a and pAr15834b. Kanamycin-resistant transconjugants belonging to a second class were avirulent and contained an altered form of pAr15834b. Strain 15834 can utilize octopine. However, this trait was not detected in any of the transconjugants. Octopine is not synthesized by infected plant tissue.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

White FF,Nester EW

doi

10.1128/JB.141.3.1134-1141.1980

subject

Has Abstract

pub_date

1980-03-01 00:00:00

pages

1134-41

issue

3

eissn

0021-9193

issn

1098-5530

journal_volume

141

pub_type

杂志文章
  • Magnesium starvation of Aerobacter aerogenes. II. Rates of nucleic acid synthesis and methods for their measurement.

    abstract::The rates of synthesis of Aerobacter aerogenes nucleic acids were estimated during incubation of the bacteria in a Mg(++)-free medium. Deoxyribonucleic acid (DNA) synthesized during Mg(++) starvation, or in the preceding exponential growth, remained acid-precipitable for 2.5 hr before breaking down to acid-soluble pro...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.93.1.345-356.1967

    authors: Kennell D,Kotoulas A

    更新日期:1967-01-01 00:00:00

  • Autogenous transcriptional regulation of the regA gene, encoding an AraC-Like, essential virulence regulator in Citrobacter rodentium.

    abstract::We identified several promoters responsible for the expression of regA, which encodes a global virulence regulator in Citrobacter rodentium. Expression of some of the promoters was strongly autoactivated by RegA in conjunction with bicarbonate. Biochemical and mutational analyses were used to determine the consensus s...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01224-10

    authors: Tan A,Yang J,Tauschek M,Praszkier J,Robins-Browne RM

    更新日期:2011-04-01 00:00:00

  • The adsorption protein genes of Xanthomonas campestris filamentous phages determining host specificity.

    abstract::Gene III (gIII) of phiLf, a filamentous phage specifically infecting Xanthomonas campestris pv. campestris, was previously shown to encode a virion-associated protein (pIII) required for phage adsorption. In this study, the transcription start site for the gene and the N-terminal sequence of the protein were determine...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.181.8.2465-2471.1999

    authors: Lin NT,Liu TJ,Lee TC,You BY,Yang MH,Wen FS,Tseng YH

    更新日期:1999-04-01 00:00:00

  • Genetic determinants of a nickel-specific transport system are part of the plasmid-encoded hydrogenase gene cluster in Alcaligenes eutrophus.

    abstract::Nickel-deficient (Nic-) mutants of Alcaligenes eutrophus requiring high levels of nickel ions for autotrophic growth with hydrogen were characterized. The Nic- mutants carried defined deletions in the hydrogenase gene cluster of the indigenous pHG megaplasmid. Nickel deficiency correlated with a low level of the nicke...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.3.1340-1345.1989

    authors: Eberz G,Eitinger T,Friedrich B

    更新日期:1989-03-01 00:00:00

  • HMW1 is required for stability and localization of HMW2 to the attachment organelle of Mycoplasma pneumoniae.

    abstract::The cytoskeletal proteins HMW1 and HMW2 are components of the terminal organelle of the cell wall-less bacterium Mycoplasma pneumoniae. HMW1 is required for a tapered, filamentous morphology but exhibits accelerated turnover in the absence of HMW2. Here, we report that a reciprocal dependency exists between HMW1 and H...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.186.24.8221-8228.2004

    authors: Willby MJ,Balish MF,Ross SM,Lee KK,Jordan JL,Krause DC

    更新日期:2004-12-01 00:00:00

  • Whole-genome sequence of Streptococcus pseudopneumoniae isolate IS7493.

    abstract::Streptococcus pseudopneumoniae is a member of the viridans group streptococci (VGS) whose pathogenic significance is unclear. We announce the complete genome sequence of S. pseudopneumoniae IS7493. The genome sequence will assist in the characterization of this new organism and facilitate the development of accurate d...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.06075-11

    authors: Shahinas D,Tamber GS,Arya G,Wong A,Lau R,Jamieson F,Ma JH,Alexander DC,Low DE,Pillai DR

    更新日期:2011-11-01 00:00:00

  • Effects of carbon source and growth rate on cell wall composition of Bacillus subtilis subsp. niger.

    abstract::A study was made to determine whether factors other than the availability of phosphorus were involved in the regulation of synthesis of teichoic and teichuronic acids in Bacillus subtilis subsp. niger WM. First, the nature of the carbon source was varied while the dilution rate was maintained at about 0.3 h-1. Irrespe...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.144.1.238-246.1980

    authors: Kruyssen FJ,de Boer WR,Wouters JT

    更新日期:1980-10-01 00:00:00

  • Method for restricting incorporation of radioactivity from 3 H-thymidine into deoxyribonucleic acid only during outgrowth of spores of Bacillus cereus T.

    abstract::When heat-activated spores of Bacillus cereus T (thy(-)) were germinated and grown in medium containing (3)H-thymidine, a significant amount of radioactivity was incorporated into ribonucleic acid and deoxyribonucleic acid (DNA). A method was developed to restrict the incorporation of radioactivity from (3)H-thymidine...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.109.2.599-605.1972

    authors: Rana RS,Halvorson HO

    更新日期:1972-02-01 00:00:00

  • Distribution of Tn551 insertion sites responsible for auxotrophy on the Staphylococcus aureus chromosome.

    abstract::A method was devised to efficiently select isolates of Staphylococcus aureus 8325 in which Tn551, a transposon originating on the pI258 plasmid responsible for erythromycin resistance (Emr), had translocated to the host chromosome. This method consisted of selecting for Emr at 43 degrees C with a strain in which the p...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.145.1.479-488.1981

    authors: Pattee PA

    更新日期:1981-01-01 00:00:00

  • Molecular characterization of the Salmonella enterica serovar Typhi Vi-typing bacteriophage E1.

    abstract::Some bacteriophages target potentially pathogenic bacteria by exploiting surface-associated virulence factors as receptors. For example, phage have been identified that exhibit specificity for Vi capsule producing Salmonella enterica serovar Typhi. Here we have characterized the Vi-associated E1-typing bacteriophage u...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01654-07

    authors: Pickard D,Thomson NR,Baker S,Wain J,Pardo M,Goulding D,Hamlin N,Choudhary J,Threfall J,Dougan G

    更新日期:2008-04-01 00:00:00

  • Organization of the genes for protein synthesis elongation factors Tu and G in the cyanobacterium Anacystis nidulans.

    abstract::The genes for protein synthesis elongation factors Tu and G were cloned from the cyanobacterium Anacystis nidulans. The locations of these genes were mapped within the cloned DNA fragment by hybridization with Escherichia coli probes. The organization of the cloned fragment and the DNA flanking it in the A. nidulans c...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.166.1.78-82.1986

    authors: Mickel FS,Spremulli LL

    更新日期:1986-04-01 00:00:00

  • Partial characterization of lipid A of intraperiplasmically grown Bdellovibrio bacteriovorus.

    abstract::The lipid A components of substrate cell origin incorporated by Bdellovibrio bacteriovorus during intraperiplasmic growth (D. R. Nelson and S. C. Rittenberg, J. Bacteriol. 147:860-868, 1981) were shown to be integrated into its lipopolysaccharide structure. Lipid A isolated from bdellovibrios grown on Escherichia coli...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.147.3.869-874.1981

    authors: Nelson DR,Rittenberg SC

    更新日期:1981-09-01 00:00:00

  • Determining the DNA sequence elements required for binding integration host factor to two different target sites.

    abstract::Binding sites for the Escherichia coli protein integration host factor (IHF) include a set of conserved bases that can be summarized by the consensus sequence WATCAANNNNTTR (W is dA or dT, R is dA or dG, and N is any nucleotide). However, additional 5'-proximal bases, whose common feature is a high dA+dT content, are ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.10.2999-3006.1994

    authors: Hales LM,Gumport RI,Gardner JF

    更新日期:1994-05-01 00:00:00

  • Propanediol utilization genes (pdu) of Salmonella typhimurium: three genes for the propanediol dehydratase.

    abstract::The propanediol utilization (pdu) operon of Salmonella typhimurium encodes proteins required for the catabolism of propanediol, including a coenzyme B12-dependent propanediol dehydratase. A clone that expresses propanediol dehydratase activity was isolated from a Salmonella genomic library. DNA sequence analysis showe...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.21.6633-6639.1997

    authors: Bobik TA,Xu Y,Jeter RM,Otto KE,Roth JR

    更新日期:1997-11-01 00:00:00

  • Amino acid substitution in the lactose carrier protein with the use of amber suppressors.

    abstract::Five lacY mutants with amber stop codons at known positions were each placed into 12 different suppressor strains. The 60 amino acid substitutions obtained in this manner were tested for growth on lactose-minimal medium plates and for transport of lactose, melibiose, and thiomethylgalactoside. Most of the amino acid s...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.16.5436-5441.1992

    authors: Huang AM,Lee JI,King SC,Wilson TH

    更新日期:1992-08-01 00:00:00

  • The Sakaguchi reaction product quenches phycobilisome fluorescence, allowing determination of the arginine concentration in cells of Anabaena strain PCC 7120.

    abstract::The filamentous cyanobacterium Anabaena fixes nitrogen in specialized cells called heterocysts. The immediate product of fixation, ammonia, is known to be assimilated by addition to glutamate to make glutamine. How fixed nitrogen is transported along the filament to the 10 to 20 vegetative cells that separate heterocy...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01512-12

    authors: Ke S,Haselkorn R

    更新日期:2013-01-01 00:00:00

  • Characterization of the Synechocystis strain PCC 6803 penicillin-binding proteins and cytokinetic proteins FtsQ and FtsW and their network of interactions with ZipN.

    abstract::Because very little is known about cell division in noncylindrical bacteria and cyanobacteria, we investigated 10 putative cytokinetic proteins in the unicellular spherical cyanobacterium Synechocystis strain PCC 6803. Concerning the eight penicillin-binding proteins (PBPs), which define three classes, we found that S...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00620-09

    authors: Marbouty M,Mazouni K,Saguez C,Cassier-Chauvat C,Chauvat F

    更新日期:2009-08-01 00:00:00

  • Nitrogen catabolite repression in a glutamate auxotroph of Saccharomyces cerevisiae.

    abstract::The biosynthesis of asparaginase II in Saccharomyces cerevisiae is subject to nitrogen catabolite repression. In the present study we examined the physiological effects of glutamate auxotrophy on cellular metabolism and on the nitrogen catabolite repression of asparaginase II. Glutamate auxotrophic cells, incubated wi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.151.1.29-35.1982

    authors: Kang L,Keeler ML,Dunlop PC,Roon RJ

    更新日期:1982-07-01 00:00:00

  • Amphilphilic nature of spiralin, the major protein of the Spiroplasma citri cell membrane.

    abstract::Spiralin could not be solubilized in the absence of detergents, and it was shown by charge-shift crossed immunoelectrophoresis that this protein was capable of binding detergents under nondenaturing conditions. These properties indicate the amphiphilic nature of spiralin, which therefore should be regarded as an intri...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.140.2.738-741.1979

    authors: Wróblewski H

    更新日期:1979-11-01 00:00:00

  • Evidence for a chemiosmotic model of dehalorespiration in Desulfomonile tiedjei DCB-1.

    abstract::Desulfomonile tiedjei DCB-1, a sulfate-reducing bacterium, conserves energy for growth from reductive dehalogenation of 3-chlorobenzoate by an uncharacterized chemiosmotic process. Respiratory electron transport components were examined in D. tiedjei cells grown under conditions for reductive dehalogenation, pyruvate ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.181.1.40-46.1999

    authors: Louie TM,Mohn WW

    更新日期:1999-01-01 00:00:00

  • Developmental control of glucosamine and galactosamine levels during conidation in Neurospora crassa.

    abstract::The glucosamine and galactosamine content of mycelia was measured in cultures of Neurospora crassa grown on the surface of dialysis membranes. The glucosamine content was relatively constant throughout the different regions of the mycelial mat. The galactosamine content, however, was always lower in the growing-front ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.122.3.1071-1075.1975

    authors: Schmit JC,Brody S

    更新日期:1975-06-01 00:00:00

  • A 53-base-pair inverted repeat negatively regulates expression of the adjacent and divergently oriented cytochrome P450(BM-1) gene and its regulatory gene, bm1P1, in Bacillus megaterium.

    abstract::To study the role of the cis-acting element(s) in controlling the expression of the cytochrome P450(BM-1) gene and its upstream regulatory gene, bm1P1, in Bacillus megaterium, various deletion derivatives were constructed. A 53-bp inverted repeat located midway between the P450(BM-1) gene and bm1P1 gene was found in v...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.1.280-283.1997

    authors: Shaw GC,Sung CC,Liu CH,Kao HS

    更新日期:1997-01-01 00:00:00

  • Identification of [2Fe-2S] clusters in microbial ferrochelatases.

    abstract::The terminal enzyme of heme biosynthesis, ferrochelatase (EC 4.99.1.1), catalyzes the insertion of ferrous iron into protoporphyrin IX to form protoheme. Prior to the present work, [2Fe-2S] clusters have been identified and characterized in animal ferrochelatases but not in plant or prokaryotic ferrochelatases. Herein...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.184.9.2460-2464.2002

    authors: Dailey TA,Dailey HA

    更新日期:2002-05-01 00:00:00

  • A Salmonella typhimurium mutant dependent upon carbamyl aspartate for resistance to 5-fluorouracil is specifically affected in ubiquinone biosynthesis.

    abstract::The isolation and properties of a mutant dependent upon exogenous carbamyl aspartate for resistance to 5-fluorouracil are described. The mutant was deficient in the synthesis of ubiquinone and accumulated a quinone provisionally identified as the ubiquinone precursor 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinone. T...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.145.2.1095-1098.1981

    authors: Zak VL,Kelln RA

    更新日期:1981-02-01 00:00:00

  • Bacillus subtilis mutant allele sup-3 causes lysine insertion at ochre codons: use of sup-3 in studies of translational attenuation.

    abstract::The mutation sup-3 in Bacillus subtilis suppresses ochre (TAA) mutations at each of three codons in the 5' end of the cat-86 coding sequence. The suppressor is shown to insert lysine at ochre codons. The efficiency of suppression by sup-3 is about 15%, as determined by changing a cat-86 Lys codon (codon 12) to an ochr...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.10.5322-5324.1989

    authors: Mulbry WW,Ambulos NP Jr,Lovett PS

    更新日期:1989-10-01 00:00:00

  • Differential proteomic analysis of the Bacillus anthracis secretome: distinct plasmid and chromosome CO2-dependent cross talk mechanisms modulate extracellular proteolytic activities.

    abstract::The secretomes of a virulent Bacillus anthracis strain and of avirulent strains (cured of the virulence plasmids pXO1 and pXO2), cultured in rich and minimal media, were studied by a comparative proteomic approach. More than 400 protein spots, representing the products of 64 genes, were identified, and a unique patter...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.188.10.3551-3571.2006

    authors: Chitlaru T,Gat O,Gozlan Y,Ariel N,Shafferman A

    更新日期:2006-05-01 00:00:00

  • Mechanism of D-cycloserine action: alanine racemase from Escherichia coli W.

    abstract::The antibiotic d-cycloserine is an effective inhibitor of alanine racemase. The lack of inhibition by l-cycloserine of alanine racemase from Staphylococcus aureus led Roze and Strominger to formulate the cycloserine hypothesis. This hypothesis states that d-cycloserine has the conformation required of the substrates o...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.110.3.978-987.1972

    authors: Lambert MP,Neuhaus FC

    更新日期:1972-06-01 00:00:00

  • The carboxy-terminal portion of the CheA kinase mediates regulation of autophosphorylation by transducer and CheW.

    abstract::The CheA kinase is a central protein in the signal transduction network that controls chemotaxis in Escherichia coli. CheA receives information from a transmembrane receptor (e.g., Tar) and CheW proteins and relays it to the CheB and CheY proteins. The biochemical activities of CheA proteins truncated at various dista...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.7.2097-2101.1993

    authors: Bourret RB,Davagnino J,Simon MI

    更新日期:1993-04-01 00:00:00

  • Mu-like prophage strong gyrase site sequences: analysis of properties required for promoting efficient mu DNA replication.

    abstract::The bacteriophage Mu genome contains a centrally located strong gyrase site (SGS) that is required for efficient prophage replication. To aid in studying the unusual properties of the SGS, we sought other gyrase sites that might be able to substitute for the SGS in Mu replication. Five candidate sites were obtained by...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.186.14.4575-4584.2004

    authors: Oram M,Pato ML

    更新日期:2004-07-01 00:00:00

  • Chromosomal integration, tandem amplification, and deamplification in Pseudomonas putida F1 of a 105-kilobase genetic element containing the chlorocatechol degradative genes from Pseudomonas sp. Strain B13.

    abstract::Analysis of chlorobenzene-degrading transconjugants of Pseudomonas putida F1 which had acquired the genes for chlorocatechol degradation (clc) from Pseudomonas sp. strain B13 revealed that the clc gene cluster was present on a 105-kb amplifiable genetic element (named the clc element). In one such transconjugant, P. p...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.17.4360-4369.1998

    authors: Ravatn R,Studer S,Springael D,Zehnder AJ,van der Meer JR

    更新日期:1998-09-01 00:00:00