Abstract:
:DifA is a methyl-accepting chemotaxis protein (MCP)-like sensory transducer that regulates exopolysaccharide (EPS) production in Myxococcus xanthus. Here mutational analysis and molecular biology were used to probe the signaling mechanisms of DifA in EPS regulation. We first identified the start codon of DifA experimentally; this identification extended the N terminus of DifA for 45 amino acids (aa) from the previous bioinformatics prediction. This extension helped to address the outstanding question of how DifA receives input signals from type 4 pili without a prominent periplasmic domain. The results suggest that DifA uses its N-terminus extension to sense an upstream signal in EPS regulation. We suggest that the perception of the input signal by DifA is mediated by protein-protein interactions with upstream components. Subsequent signal transmission likely involves transmembrane signaling instead of direct intramolecular interactions between the input and the output modules in the cytoplasm. The basic functional unit of DifA for signal transduction is likely dimeric as mutational alteration of the predicted dimeric interface of DifA significantly affected EPS production. Deletions of 14-aa segments in the C terminus suggest that the newly defined flexible bundle subdomain in MCPs is likely critical for DifA function because shortening of this bundle can lead to constitutively active mutations.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Xu Q,Black WP,Nascimi HM,Yang Zdoi
10.1128/JB.00944-10subject
Has Abstractpub_date
2011-02-01 00:00:00pages
759-67issue
3eissn
0021-9193issn
1098-5530pii
JB.00944-10journal_volume
193pub_type
杂志文章abstract::Chloroflexus, a newly described genus of filamentous, photosynthetic, gliding bacteria, oxidizes sulfide anaerobically under photoautotrophic or photoheterotrophic growh conditions and deposits elemental sulfur outside the cell. The formation of sulfur granules outside the cell supports the idea that this organism is ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.122.2.782-784.1975
更新日期:1975-05-01 00:00:00
abstract::The gene specifying a membrane-bound nucleoside diphosphate sugar hydrolase of Salmonella typhimurium was mapped near the metA locus by using intergeneric crosses between this bacterium and Escherichia coli. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.137.3.1428-1429.1979
更新日期:1979-03-01 00:00:00
abstract::A transformable strain of Bacillus subtilis 6160, a derivative of B. subtilis 168, produces three kinds of casein hydrolytic enzymes (alkaline protease, neutral protease, and esterase) in a culture medium. B. natto IAM 1212 produces 15 to 20 times as much total proteolytic activity as does B. subtilis. Extracellular p...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.119.1.82-91.1974
更新日期:1974-07-01 00:00:00
abstract::Mycoplasma pneumoniae exhibits a novel form of gliding motility that is mediated by the terminal organelle, a differentiated polar structure. Given that genes known to be involved in gliding in other organisms are absent in M. pneumoniae, random transposon mutagenesis was employed to generate mutants with gliding-defi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.02277-12
更新日期:2013-04-01 00:00:00
abstract::A novel genetic scheme allowed us to isolate extragenic suppressor mutations that restored mutant OmpF assembly. One group of these mutations, termed asmB for assembly suppressor mutation B, permitted mutant OmpF assembly in a non-allele-specific manner. Genetic mapping analyses placed the asmB mutations at the 2-min ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.17.5138-5143.1996
更新日期:1996-09-01 00:00:00
abstract::Manganese is accumulated in Bacillus subtilis by a highly specific active transport system. This trace element "pump" is insensitive to added magnesium or calcium and preferentially accumulates manganese in the presence of cobalt, iron, and copper. Manganese uptake in B. subtilis is inhibited by cyanide, azide, pentac...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.113.3.1363-1372.1973
更新日期:1973-03-01 00:00:00
abstract::Kinsky, Stephen C. (Washington University, St. Louis, Mo.). Induction and repression of nitrate reductase in Neurospora crassa. J. Bacteriol. 82:898-904. 1961.-Techniques are described for studying induced enzyme formation in Neurospora crassa. The effects of various parameters (time, pH, nitrate concentration, etc.) ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.82.6.898-904.1961
更新日期:1961-12-01 00:00:00
abstract::The pH dependence of transport of the neutral amino acids L-serine and L-alanine by membrane vesicles of Streptococcus cremoris have been studied in detail. The rates of four modes of facilitated diffusion (e.g., influx, efflux, exchange, and counterflow) of L-serine and L-alanine increase with increasing H+ concentra...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.6.2748-2754.1987
更新日期:1987-06-01 00:00:00
abstract::Sinorhizobium meliloti is a soil-dwelling alphaproteobacterium that engages in a nitrogen-fixing root nodule symbiosis with leguminous plants. Cell surface polysaccharides are important both for adapting to stresses in the soil and for the development of an effective symbiotic interaction. Among the polysaccharides ch...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00501-17
更新日期:2018-01-10 00:00:00
abstract::ShdA is a large outer membrane protein of the autotransporter family whose passenger domain binds the extracellular matrix proteins fibronectin and collagen I, possibly by mimicking the host ligand heparin. The ShdA passenger domain consists of approximately 1,500 amino acid residues that can be divided into two regio...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.186.15.4931-4939.2004
更新日期:2004-08-01 00:00:00
abstract::Klebsiella strain RE1544 contains two lac operons, one on the chromosome and one on a lac plasmid. A mutant of RE1544, in which the lacZ genes of both operons produce no active enzyme, was found to synthesize a beta-galactosidase that hydrolyzes ortho-nitrophenyl-beta-D-galactopyranoside but not lactose. Synthesis of ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.132.1.219-223.1977
更新日期:1977-10-01 00:00:00
abstract::HlyD, a member of the membrane fusion protein family, is essential for the secretion of the RTX hemolytic toxin HlyA from Escherichia coli. Random point mutations affecting HlyA secretion were obtained, distributed in most periplasmic regions of the HlyD molecule. Analysis of the secretion phenotypes of different muta...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.21.7471-7480.2005
更新日期:2005-11-01 00:00:00
abstract::Two-dimensional gel analyses were made of proteins synthesized in Escherichia coli during various O2- -generating conditions. Nine proteins were constitutively synthesized over wild-type levels in superoxide dismutase (sodA sodB) double mutants. Addition of redox cycling agents such as paraquat and plumbagin at variou...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.3.1476-1484.1989
更新日期:1989-03-01 00:00:00
abstract::The ribosomal proteins from nine species of prokaryotes have been compared by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate. The stained gels were scanned spectrophotometrically, the weight and number average molecular weights were calculated, and the detailed distribution of the protein...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.111.2.474-480.1972
更新日期:1972-08-01 00:00:00
abstract::Microcin J25 (MccJ25) is a small peptide antibiotic produced by an Escherichia coli strain isolated from human feces. The genetic determinants for MccJ25 synthesis and immunity have been cloned from the low-copy-number wild-type plasmid pTUC1OO into the compatible vectors pBR322 and pACYC184. Physical and phenotypical...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.12.3661-3663.1996
更新日期:1996-06-01 00:00:00
abstract::Smith, Paul F. (University of Pennsylvania, Philadelphia) and George H. Rothblat. Comparison of pleuropneumonia-like and L-type organisms. J. Bacteriol. 83:500-506. 1962.-The content of total lipid and nonsaponifiable lipid depended upon the type of organism; the greatest amounts were found in pleuropneumonia-like org...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.83.3.500-506.1962
更新日期:1962-03-01 00:00:00
abstract::Motility of bacteria like Salmonella enterica is a highly regulated process that responds to a variety of internal and external stimuli. A hierarchy of three promoter classes characterizes the Salmonella flagellar system, and the onset of flagellar gene expression depends on the oligomeric regulatory complex and class...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00728-13
更新日期:2013-09-01 00:00:00
abstract::The effect of plasmid CAM-OCT on responses to UV irradiation was compared in Pseudomonas aeruginosa, in Pseudomonas putida, and in Pseudomonas putida mutants carrying mutations in UV response genes. CAM-OCT substantially increased both survival and mutagenesis in the two species. P. aeruginosa strains without CAM-OCT ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.2.975-982.1989
更新日期:1989-02-01 00:00:00
abstract::A major phenotypic trait of the Mycobacterium avium complex is the ability to produce rough and smooth colony variants. The chemical basis of this morphological variation is the loss of an antigenic surface structure, termed glycopeptidolipid (GPL), by rough variants. Using M. avium serovar 2 strain 2151 as a model sy...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.21.6177-6182.2000
更新日期:2000-11-01 00:00:00
abstract::Burkholderia thailandensis is a soil saprophyte that is closely related to the pathogen Burkholderia pseudomallei, the etiological agent of melioidosis in humans. The environmental niches and infection sites occupied by these bacteria are thought to contain only limited concentrations of oxygen, where they can generat...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00304-20
更新日期:2020-11-04 00:00:00
abstract::We show that hisT function is required for normal growth of Escherichia coli K-12, since a lack of hisT-mediated pseudouridine tRNA modification causes a uracil requirement that interferes with cell division. We also show that hisT transcription is positively growth rate regulated in exponentially growing bacteria and...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.22.7395-7400.1991
更新日期:1991-11-01 00:00:00
abstract::To isolate strains with new recA mutations that differentially affect RecA protein functions, we mutagenized in vitro the recA gene carried by plasmid mini-F and then introduced the mini-F-recA plasmid into a delta recA host that was lysogenic for prophage phi 80 and carried a lac duplication. By scoring prophage indu...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.5.2415-2423.1989
更新日期:1989-05-01 00:00:00
abstract::Escherichia coli has a formate hydrogenlyase system which allows it to maintain an electron balance during anaerobic growth by passing electrons from formate to H+ ions, thus generating H2. The Mu d1(Ap lac) bacteriophage was used to generate mutants that were defective in passing electrons from formate to benzyl viol...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.158.1.180-186.1984
更新日期:1984-04-01 00:00:00
abstract::A number of gram-negative bacteria have a quorum-sensing system and produce N-acyl-l-homoserine lactone (AHL) that they use them as a quorum-sensing signal molecule. Pantoea ananatis is reported as a common colonist of wheat heads at ripening and causes center rot of onion. In this study, we demonstrated that P. anana...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01054-07
更新日期:2007-11-01 00:00:00
abstract::The hook-basal body (HBB) is a key intermediate structure in the flagellar assembly pathway in Salmonella typhimurium. The FlgM protein inhibits the flagellum-specific transcription factor sigma28 in the absence of the intact HBB structure and is secreted out of the cell following HBB completion. The flk gene encodes ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.20.5384-5397.1998
更新日期:1998-10-01 00:00:00
abstract::The overlapping fdhA and fdhB genes of Methanobacterium formicicum, which encode the alpha and beta subunits, respectively, of formate dehydrogenase, were cotranscribed as part of an approximately 4.5-kb transcript. An additional gene (fdhC) upstream of fdhA was cotranscribed with fdhA and fdhB. The deduced amino acid...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.15.4997-5004.1992
更新日期:1992-08-01 00:00:00
abstract::Cnm is a surface-associated protein present in a subset of Streptococcus mutans strains that mediates binding to extracellular matrices, intracellular invasion, and virulence. Here, we showed that cnm transcription is controlled by the global regulators CovR and VicRKX. In silico analysis identified multiple putative ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00141-18
更新日期:2018-11-06 00:00:00
abstract::The Bacillus subtilis anti-TRAP protein regulates the ability of the tryptophan-activated TRAP protein to bind to trp operon leader RNA and promote transcription termination. AT synthesis is regulated both transcriptionally and translationally by uncharged tRNA(Trp). In this study, we examined the roles of AT synthesi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01820-07
更新日期:2008-03-01 00:00:00
abstract::The opportunistic pathogen Pseudomonas aeruginosa utilizes a type III secretion system (T3SS) to evade phagocytosis and damage eukaryotic cells. Transcription of the T3SS regulon is controlled by ExsA, a member of the AraC/XylS family of transcriptional regulators. These family members generally consist of an approxim...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00002-09
更新日期:2009-06-01 00:00:00
abstract::When titanium(III) citrate was used as electron donor for the reduction of methyl coenzyme M by the methyl coenzyme M methylreductase system of Methanobacterium thermoautotrophicum delta H, component A1 was no longer required. The simpler system thus obtained required components A2, A3, and C as well as catalytic amou...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.9.3946-3952.1988
更新日期:1988-09-01 00:00:00