Synthesis and localization of the Salmonella SPI-1 type III secretion needle complex proteins PrgI and PrgJ.

Abstract:

:An essential component of type III secretion systems (TTSS) is a supramolecular structure termed the needle complex. In Salmonella enterica, at least four proteins make up this structure: InvG, PrgH, PrgK, and PrgI. Another protein, PrgJ, is thought to play a role in the assembly of this structure, but its function is poorly understood. We have analyzed the expression and localization of PrgJ and the needle protein PrgI in different S. enterica serovar Typhimurium mutant strains. We found that the levels of PrgI and PrgJ were significantly reduced in a TTSS-deficient invA mutant strain and that the decreased levels were due to protein instability. In addition, we found that PrgJ, although associated with the needle complex in wild-type S. enterica serovar Typhimurium, was absent from needle complexes obtained from an invJ mutant strain, which exhibits very long needle substructures. We suggest that PrgJ is involved in capping the needle substructure of the needle complex.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Sukhan A,Kubori T,Galán JE

doi

10.1128/jb.185.11.3480-3483.2003

keywords:

subject

Has Abstract

pub_date

2003-06-01 00:00:00

pages

3480-3

issue

11

eissn

0021-9193

issn

1098-5530

journal_volume

185

pub_type

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