Stable denaturation of chromosomal DNA from Saccharomyces cerevisiae during meiosis.

Abstract:

:Partial denaturation of Saccharomyces cerevisiae chromosomal DNA was found to occur spontaneously during meiosis. Short regions of strand separation (300 base pairs long) were seen in DNA molecules prepared for electron microscopy by the aqueous spreading technique. These regions were clustered along the DNA. The time course of their appearance indicated that the denatured regions were present during the periods of premeiotic DNA replication and recombination. A similar pattern of denaturation was also detected in the DNA from vegetatively grown cells of a conditional cdc8 mutant, which is defective in DNA replication.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Klein HL,Byers B

doi

10.1128/JB.134.2.629-635.1978

subject

Has Abstract

pub_date

1978-05-01 00:00:00

pages

629-35

issue

2

eissn

0021-9193

issn

1098-5530

journal_volume

134

pub_type

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