Transposon Tn917lacZ mutagenesis of Bacillus subtilis: identification of two new loci required for motility and chemotaxis.

abstract：:Two proteins that bind penicillin were observed in Escherichia coli infected with lambda phages 141, 142, 650, and 651 from the Kohara genomic library. These phages carry chromosomal DNA fragments that do not contain any known penicillin binding protein (PBP) genes, indicating that unrecognized gene products were exhi...

journal_title：Journal of bacteriology

authors： Henderson TA,Young KD,Denome SA,Elf PK

更新日期：1997-10-01 00:00:00

abstract：:The alpha (62,000-dalton) and beta (49,000-dalton) subunits of Methanosarcina barkeri ATPase were purified to homogeneity. The subunits and ATPase complex were trypsinized in the presence of various nucleotides. ATP and ADP changed the trypsin sensitivity of the alpha subunit in the complex and isolated forms, suggest...

journal_title：Journal of bacteriology

authors： Inatomi K,Maeda M

更新日期：1988-12-01 00:00:00

abstract：:CTXphi is a filamentous bacteriophage that encodes cholera toxin. CTXphi infection of its host bacterium, Vibrio cholerae, requires the toxin-coregulated pilus (TCP) and the products of the V. cholerae tolQRA genes. Here, we have explored the role of OrfU, a predicted CTXphi minor coat protein, in CTXphi infection. Pr...

journal_title：Journal of bacteriology

authors： Heilpern AJ,Waldor MK

更新日期：2003-02-01 00:00:00

abstract：:The essential function of penicillin-binding protein 2 (PBP2) in methicillin-susceptible Staphylococcus aureus RN4220 was clearly established by placing the pbp2 gene under control of the inducible P(spac) promoter; the resulting bacteria were unable to grow in the absence of inducer. In contrast, the deficit in PBP2 ...

journal_title：Journal of bacteriology

authors： Pinho MG,Filipe SR,de Lencastre H,Tomasz A

更新日期：2001-11-01 00:00:00

abstract：:Application of in vivo expression technology (IVET) to Yersinia ruckeri, an important fish pathogen, allowed the identification of two adjacent genes that represent a novel bacterial system involved in the uptake and degradation of l-cysteine. Analysis of the translational products of both genes showed permease domain...

journal_title：Journal of bacteriology

authors： Méndez J,Reimundo P,Pérez-Pascual D,Navais R,Gómez E,Guijarro JA

更新日期：2011-02-01 00:00:00

abstract：:The site of integration and the molecular orientation of a prophage Mu integrated within the resistance transfer factor component of plasmid R100-1 have been determined on the physical map of the plasmid. This allowed us (i) to determine the direction of deoxyribonucleic acid transfer from oriT during conjugation and ...

journal_title：Journal of bacteriology

authors： Legrand P,Bouche JP,Louarn JM

更新日期：1979-12-01 00:00:00

abstract：:Two strains of Staphylococcus aureus (PC1 and 196E), when grown in medium containing 0.002% sodium dodecyl sulfate (SDS), showed from 96.1 to 100% loss of the ability to produce penicillinase. Resistance to cadmium and zinc was lost concomitantly with the ability to produce penicillinase. A comparison of the rate of c...

journal_title：Journal of bacteriology

authors： Sonstein SA,Baldwin JN

更新日期：1972-01-01 00:00:00

abstract：:Entry into intestinal epithelial cells is an essential step in the pathogenesis of Salmonella infections. Our laboratory has previously identified a genetic locus, inv, that is necessary for efficient entry of Salmonella typhimurium into cultured epithelial cells. We have carried out a molecular and functional analysi...

journal_title：Journal of bacteriology

authors： Eichelberg K,Ginocchio CC,Galán JE

更新日期：1994-08-01 00:00:00

abstract：:Biofilm formation on catheters is thought to contribute to persistence of catheter-associated urinary tract infections (CAUTI), which represent the most frequent nosocomial infections. Knowledge of genetic factors for catheter colonization is limited, since their role has not been assessed using physicochemical condit...

journal_title：Journal of bacteriology

authors： Reisner A,Maierl M,Jörger M,Krause R,Berger D,Haid A,Tesic D,Zechner EL

更新日期：2014-03-01 00:00:00

abstract：:At least six phenotypically distinct classes of mutants of Escherichia coli which require serine or pyridoxine or both can be isolated. Three of the six classes lack 3-phosphoserine-2-oxoglutarate aminotransferase. One of these classes contains WG5, a mutant previously characterized as containing the pdxF5 allele. The...

journal_title：Journal of bacteriology

authors： Dempsey WB,Ito H

更新日期：1970-11-01 00:00:00

abstract：:Expression levels of Bacillus amyloliquefaciens alpha-amylase, Escherichia coli TEM-beta-lactamase, and Semliki Forest virus glycoprotein E1 genes were compared in Bacillus subtilis. All three model genes were expressed by using a secretion vector, constructed by joining the B. amyloliquefaciens alpha-amylase promoter...

journal_title：Journal of bacteriology

authors： Ulmanen I,Lundström K,Lehtovaara P,Sarvas M,Ruohonen M,Palva I

更新日期：1985-04-01 00:00:00

abstract：:Klein, Dolph (Rutgers, The State University, New Brunswick, N. J.), and David Pramer. Bacterial dissimilation of streptomycin. J. Bacteriol. 82:505-510. 1961.-The influence of various nutritional and environmental factors on the dissimilation of streptomycin by a pseudomonad isolated from soil was investigated, and co...

journal_title：Journal of bacteriology

authors： KLEIN D,PRAMER D

更新日期：1961-10-01 00:00:00

abstract：:An assay modeled on a known polymorphism in the PE_PGRS9 gene of Mycobacterium tuberculosis was designed to assess the mutability of a sequence containing interspersed PGRS repeats. Application of the assay in Mycobacterium smegmatis revealed sequence plasticity: in addition to recapitulating the mutation on which it ...

journal_title：Journal of bacteriology

authors： Machowski EE,Barichievy S,Springer B,Durbach SI,Mizrahi V

更新日期：2007-03-01 00:00:00

abstract：:Birnbaum, Jerome (University of Cincinnati, Cincinnati, Ohio), and Herman C. Lichstein. Metabolism of biotin and analogues of biotin by microorganisms. IV. Degradation of biotin, oxybiotin, and desthiobiotin by Lactobacillus casei. J. Bacteriol. 92:925-930. 1966.-Lactobacillus casei degrades biotin when it is present ...

journal_title：Journal of bacteriology

authors： Birnbaum J,Lichstein HC

更新日期：1966-10-01 00:00:00

abstract：:Gartner, T. K. (University of California, Santa Barbara), and E. Orias. Effects of mutations to streptomycin resistance on the rate of translation of mutant genetic information. J. Bacteriol. 91:1021-1028. 1966.-The effects of mutations to streptomycin resistance of independent origin upon the translation of suppressi...

journal_title：Journal of bacteriology

authors： Gartner TK,Orias E

更新日期：1966-03-01 00:00:00

abstract：:This study investigated the apparent genetic redundancy in the biosynthesis of polyhydroxyalkanoates (PHAs) in the Rhodospirillum rubrum genome revealed by the occurrence of three homologous PHA polymerase genes (phaC1, phaC2, and phaC3). In vitro biochemical assays established that each gene product encodes PHA polym...

journal_title：Journal of bacteriology

authors： Jin H,Nikolau BJ

更新日期：2012-10-01 00:00:00

abstract：:FNR is an Escherichia coli transcription factor that regulates the transcription of many genes in response to anaerobiosis. We have constructed a series of artificial FNR-dependent promoters, based on the melR promoter, in which a consensus FNR binding site was centered at position -41.5 relative to the transcription ...

journal_title：Journal of bacteriology

authors： Barnard AM,Green J,Busby SJ

更新日期：2003-10-01 00:00:00

abstract：:Upon cold shock, Escherichia coli cell growth transiently stops. During this acclimation phase, specific cold shock proteins (CSPs) are highly induced. At the end of the acclimation phase, their synthesis is reduced to new basal levels, while the non-cold shock protein synthesis is resumed, resulting in cell growth re...

journal_title：Journal of bacteriology

authors： Yamanaka K,Inouye M

更新日期：2001-05-01 00:00:00

abstract：:The antigenic specificity of Listeria monocytogenes types I, II, III, IVa, and IVb was studied by immunochemical techniques. Immunologically active carbohydrates of the various types were extracted from cell walls and were chemically analyzed. Types I and II contained predominantly glucosamine and rhamnose; type III, ...

journal_title：Journal of bacteriology

authors： Ullmann WW,Cameron JA

更新日期：1969-05-01 00:00:00

abstract：:Vegetative cells and cysts of Azotobacter vinelandii 12837 were prepared for electron microscopy by several methods assumed to preserve structural details destroyed by techniques previously reported in the literature. Examination of large numbers of cells and cysts by these methods revealed four structural details not...

journal_title：Journal of bacteriology

authors： Vela GR,Cagle GD,Holmgren PR

更新日期：1970-11-01 00:00:00

abstract：:The penultimate step in the biosynthesis of riboflavin (vitamin B2) involves the condensation of 3,4-dihydroxy-2-butanone 4-phosphate with 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione, which is catalyzed by 6,7-dimethyl-8-ribityllumazine synthase (lumazine synthase). Pathogenic Brucella species adapted to an intr...

journal_title：Journal of bacteriology

authors： Zylberman V,Klinke S,Haase I,Bacher A,Fischer M,Goldbaum FA

更新日期：2006-09-01 00:00:00

abstract：:A cosmid library of copper-resistant (Cur) Pseudomonas syringae pv. tomato PT23 plasmid DNA was constructed and mobilized into the copper-sensitive recipient P. syringae pv. syringae PS61. One resultant cosmid clone, pCOP1 (46 kilobases), conferred copper resistance. The PT23 Cur gene(s) was located on pCOP1 by subclo...

journal_title：Journal of bacteriology

authors： Bender CL,Cooksey DA

更新日期：1987-02-01 00:00:00

abstract：:An open reading frame (slr0899) on the genome of Synechocystis sp. strain PCC 6803 encodes a polypeptide of 149 amino acid residues, the sequence of which is 40% identical to that of cyanase from Escherichia coli. Introduction into a cyanase-deficient E. coli strain of a plasmid-borne slr0899 resulted in expression of...

journal_title：Journal of bacteriology

authors： Harano Y,Suzuki I,Maeda S,Kaneko T,Tabata S,Omata T

更新日期：1997-09-01 00:00:00

abstract：:The methanol-insoluble, heat-stable enterotoxin of Escherichia coli synthesized by clinical strains or strains that harbor the cloned gene was shown to be an extracellular polypeptide. The toxin (STB) was first detected as an 8,100-Mr precursor (pre-STB) that was converted to a transiently cell-associated 5,200-Mr for...

journal_title：Journal of bacteriology

authors： Kupersztoch YM,Tachias K,Moomaw CR,Dreyfus LA,Urban R,Slaughter C,Whipp S

更新日期：1990-05-01 00:00:00

abstract：:We isolated a new recF mutant of Escherichia coli K-12 by insertion of transposon Tn5 into the recF gene. This recF400::Tn5 allele displayed the same phenotypic characteristics as the classic recF143 mutation. By using Mu d(Ap lac) fusions, the induction of nine SOS genes, including recA, umuC, dinA, dinB, dinD, dinF,...

journal_title：Journal of bacteriology

authors： Thoms B,Wackernagel W

更新日期：1987-04-01 00:00:00

abstract：:Ellar, D. J. (Syracuse University, Syracuse, N.Y.), and D. G. Lundgren. Fine structure of sporulation in Bacillus cereus grown in a chemically defined medium. J. Bacteriol. 92:1748-1764. 1966.-A study was made of the fine structure of sporulating cells of Bacillus cereus grown in a chemically defined medium. The devel...

journal_title：Journal of bacteriology

authors： Ellar DJ,Lundgren DG

更新日期：1966-12-01 00:00:00

abstract：:Ethanolamine (EA) metabolism is a trait associated with enteric pathogens, including enterohemorrhagic Escherichia coli O157:H7 (EHEC). EHEC causes severe bloody diarrhea and hemolytic uremic syndrome. EHEC encodes the ethanolamine utilization (eut) operon that allows EHEC to metabolize EA and gain a competitive advan...

journal_title：Journal of bacteriology

authors： Luzader DH,Clark DE,Gonyar LA,Kendall MM

更新日期：2013-11-01 00:00:00

abstract：:Seven mutants of Haemophilus influenzae were isolated by the criterion of sensitivity to ultraviolet (UV) inactivation of colony formation. These mutants and the wild type were characterized with regard to X-ray inactivation of colony formation, UV induction of division inhibition, the ability of the eight strains to ...

journal_title：Journal of bacteriology

authors： Setlow JK,Brown DC,Boling ME,Mattingly A,Gordon MP

更新日期：1968-02-01 00:00:00

abstract：:The frizzy (frz) genes of Myxococcus xanthus are required to control directed motility during vegetative growth and fruiting body formation. FrzCD, a protein homologous to the methyl-accepting chemotaxis proteins from enteric bacteria, is modified by methylation in response to environmental conditions. Transfer of cel...

journal_title：Journal of bacteriology

authors： McBride MJ,Zusman DR

更新日期：1993-08-01 00:00:00

abstract：:When Agrobacterium tumefaciens cells grown in the presence of tritiated thymidine to label specifically the bacterial deoxyribonucleic acid (DNA) are incubated with carrot root tissue for short periods of time, an appreciable fraction of the label becomes firmly associated with the root tissue. Such association is not...

journal_title：Journal of bacteriology

authors： Yajko DM,Hegeman GD

更新日期：1971-12-01 00:00:00