Complementation of the essential peptidoglycan transpeptidase function of penicillin-binding protein 2 (PBP2) by the drug resistance protein PBP2A in Staphylococcus aureus.

Abstract:

:The essential function of penicillin-binding protein 2 (PBP2) in methicillin-susceptible Staphylococcus aureus RN4220 was clearly established by placing the pbp2 gene under control of the inducible P(spac) promoter; the resulting bacteria were unable to grow in the absence of inducer. In contrast, the deficit in PBP2 caused by inhibition of transcription of the pbp2 gene did not block growth of a methicillin-resistant S. aureus strain expressing the extra penicillin-binding protein PBP2A, a protein of extraspecies origin that is central to the mechanism of methicillin resistance. Several lines of evidence indicate that the essential function of PBP2 that can be compensated for by PBP2A is the transpeptidase activity. This provides direct genetic evidence that PBP2A has transpeptidase activity.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Pinho MG,Filipe SR,de Lencastre H,Tomasz A

doi

10.1128/JB.183.22.6525-6531.2001

keywords:

subject

Has Abstract

pub_date

2001-11-01 00:00:00

pages

6525-31

issue

22

eissn

0021-9193

issn

1098-5530

journal_volume

183

pub_type

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