The rep mutation. III. Altered structure of the replicating Escherichia coli chromosome.

Abstract:

:The rep gene function of Escherichia coli is essential for the replication of P2 and phiX174 double-stranded deoxyribonucleic acid (DNA). Compared with isogenic rep(+) strains, rep mutants show the following characteristics: larger cell size, more DNA per cell, and a slightly lower DNA/mass ratio. The replicating rep chromosomes show a steeper gradient of marker frequencies and contain more replicating forks per chromosome. The nucleoid body of rep mutants sediments faster and contains more DNA. We deduce that the rep function is required for the "normal" replication of the E. coli chromosome and that in its absence the E. coli chromosome replicates in an altered manner, perhaps involving slower-moving replicating forks.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Lane HE,Denhardt DT

doi

10.1128/JB.120.2.805-814.1974

subject

Has Abstract

pub_date

1974-11-01 00:00:00

pages

805-14

issue

2

eissn

0021-9193

issn

1098-5530

journal_volume

120

pub_type

杂志文章
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