Abstract:
:The rep gene function of Escherichia coli is essential for the replication of P2 and phiX174 double-stranded deoxyribonucleic acid (DNA). Compared with isogenic rep(+) strains, rep mutants show the following characteristics: larger cell size, more DNA per cell, and a slightly lower DNA/mass ratio. The replicating rep chromosomes show a steeper gradient of marker frequencies and contain more replicating forks per chromosome. The nucleoid body of rep mutants sediments faster and contains more DNA. We deduce that the rep function is required for the "normal" replication of the E. coli chromosome and that in its absence the E. coli chromosome replicates in an altered manner, perhaps involving slower-moving replicating forks.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Lane HE,Denhardt DTdoi
10.1128/JB.120.2.805-814.1974subject
Has Abstractpub_date
1974-11-01 00:00:00pages
805-14issue
2eissn
0021-9193issn
1098-5530journal_volume
120pub_type
杂志文章abstract::Certain group VIIIB transition metal compounds were found to inhibit cell division in Escherichia coli, causing marked filamentous growth. Gram-negative bacilli were the most sensitive to this effect, whereas gram-positive bacilli responded only at near-toxic levels of the metal. None of the cocci tested showed any ap...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.93.2.716-721.1967
更新日期:1967-02-01 00:00:00
abstract::Sinorhizobium meliloti is a soil-dwelling alphaproteobacterium that engages in a nitrogen-fixing root nodule symbiosis with leguminous plants. Cell surface polysaccharides are important both for adapting to stresses in the soil and for the development of an effective symbiotic interaction. Among the polysaccharides ch...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00501-17
更新日期:2018-01-10 00:00:00
abstract::Bacillus subtilis cells cannot sporulate in the presence of catabolites such as glucose. During the analysis of Tn10-generated mutants, we found that deletion of the C-terminal region of the tnrA gene, which encodes a global regulator that positively regulates a number of genes in response to nitrogen limitation, resu...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.17.5009-5012.2000
更新日期:2000-09-01 00:00:00
abstract::Feldman, L. A. (Baylor University College of Medicine, Houston, Tex.), J. L. Melnick, and F. Rapp. Influence of SV40 genome on the replication of an adenovirus-SV40 "hybrid" population. J. Bacteriol. 90:778-782. 1965.-Replication of a type 7 adenovirus-SV40 hybrid population in primary African green monkey kidney cell...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.90.3.778-782.1965
更新日期:1965-09-01 00:00:00
abstract::Strain DCB-1 is a strict anaerobe capable of reductive dehalogenation. We elucidated metabolic processes in DCB-1 which may be related to dehalogenation and which further characterize the organism physiologically. Sulfoxy anions and CO2 were used by DCB-1 as catabolic electron acceptors. With suitable electron donors,...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.4.2065-2070.1990
更新日期:1990-04-01 00:00:00
abstract::Tracer studies of pyrimidine biosynthesis in Lactobacillus leichmannii (ATCC 7830) indicated that, while aspartate is utilized in the usual manner, the guanido carbon of arginine, rather than carbon dioxide, is utilized as a pyrimidine precursor. The guanido carbon of arginine also contributes, to some extent, to the ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.96.4.1249-1254.1968
更新日期:1968-10-01 00:00:00
abstract::Mutants for 9 of the 10 steps in histidine biosynthesis have been isolated and identified by enzyme assay. Each locus has been mapped in relation to the aro cluster and to other histidine loci by deoxyribonucleic acid-mediated transformation. The genes which code for enzymes 3, 6, and 8 of the pathway are linked to th...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.97.3.1444-1448.1969
更新日期:1969-03-01 00:00:00
abstract::Single-celled organisms must survive exposure to environmental extremes. Perhaps one of the most variable and potentially life-threatening changes that can occur is that of a rapid and acute decrease in external osmolarity. This easily translates into several atmospheres of additional pressure that can build up within...
journal_title:Journal of bacteriology
pub_type: 杂志文章,评审
doi:10.1128/JB.00576-12
更新日期:2012-09-01 00:00:00
abstract::Listeria monocytogenes has caused numerous human outbreaks. Here we report draft genomes of L. monocytogenes J1816 and J1-220, which belong to epidemic clones II and IV, respectively. Whole-genome sequence analysis of these strains provides a tool for studying the short-term evolution of these epidemic clones. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.05048-11
更新日期:2011-07-01 00:00:00
abstract::The tpr gene family of Treponema pallidum subsp. pallidum, the causative agent of syphilis, has recently become the focus of intensive investigation. TprF and TprI sequences are highly conserved among different isolates and are the targets of strong humoral and cellular immune responses of the host, and immunization w...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.17.6084-6093.2005
更新日期:2005-09-01 00:00:00
abstract::The Hly region from the chromosome of Vibrio cholerae El Tor strain RV79(Hly-) and the nonhemolytic classical strain 569B were cloned into plasmid vector pBR322. Escherichia coli K-12 transformants possessing these recombinant plasmids were nonhemolytic and were detected with a 32P-labeled hly-specific DNA probe. Rest...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.162.1.35-41.1985
更新日期:1985-04-01 00:00:00
abstract::Small nuclear ribonucleoprotein (snRNP) particles play an important role in the processing of pre-mRNA. snRNPs have been identified immunologically in a variety of cells, but none have ever been observed in prokaryotic systems. This report provides the first evidence for the presence of snRNP-like components in two ty...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.7.1871-1878.1993
更新日期:1993-04-01 00:00:00
abstract::Both Salmonella enterica serovar Typhimurium and Escherichia coli contain the cspH gene encoding CspH, one of the cold shock proteins (CSPs). In this study, we investigated the expression of cspH in S. enterica serovar Typhimurium and found that it was induced in response to a temperature downshift during exponential ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.19.5580-5588.2001
更新日期:2001-10-01 00:00:00
abstract::Harper, M. K. (U.S. Department of Agriculture, Washington, D.C.), H. R. Curran, and M. J. Pallansch. Properties of electrodialyzed bacterial spores. J. Bacteriol. 88:1338-1340. 1964.-Washed spores of Bacillus cereus, B. megaterium, and B. stearothermophilis suspended in distilled water were electrodialyzed at a potent...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.88.5.1338-1340.1964
更新日期:1964-11-01 00:00:00
abstract::A primer extension inhibition (toeprint) assay was developed using ribosomes and ribosomal subunits from Streptomyces lividans. This assay allowed the study of ribosome binding to streptomycete leaderless and leadered mRNA. Purified 30S subunits were unable to form a ternary complex on aph leaderless mRNA, whereas 70S...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.186.20.6864-6875.2004
更新日期:2004-10-01 00:00:00
abstract::Protein and mRNA levels of heat-labile enterotoxin (LT) of Escherichia coli are highest at 37 degrees C, and they decrease gradually as temperature is decreased. This temperature effect is eliminated in an Hns- mutant. Deletion of portions of DNA coding for the LT A subunit also results in an increase in LT expression...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.14.3715-3718.1998
更新日期:1998-07-01 00:00:00
abstract::Acinetobacter baumannii is a severe threat to human health as a frequently multidrug-resistant hospital-acquired pathogen. Part of the danger from this bacterium comes from its genome plasticity and ability to evolve quickly by taking up and recombining external DNA into its own genome in a process called natural comp...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00034-21
更新日期:2021-01-25 00:00:00
abstract::A series of mutations causing alterations in ribosomal protein S21 was mapped close to dnaG, around 66.5 min on the Escherichia coli chromosomal linkage map. They cotransduced about 95% with this marker. These mutations define gene rpsU, which is very likely the structural gene for protein S21. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.144.2.603-607.1980
更新日期:1980-11-01 00:00:00
abstract::Staphylococcus aureus was shown to transport iron complexed to a variety of hydroxamate type siderophores, including ferrichrome, aerobactin, and desferrioxamine. An S. aureus mutant defective in the ability to transport ferric hydroxamate complexes was isolated from a Tn917-LTV1 transposon insertion library after sel...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.16.4394-4400.2000
更新日期:2000-08-01 00:00:00
abstract::The mecA-27r gene from Staphylococcus aureus 27r encodes penicillin-binding protein 2a (PBP2a-27r), which causes this strain to be methicillin resistant. Removal or replacement of the N-terminal transmembrane domain had no effect on binding of penicillin, but removal of portions of the putative transglycosylase domain...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.2.443-449.1994
更新日期:1994-01-01 00:00:00
abstract::An ADP-ribosylating toxin named Aeromonas salmonicida exoenzyme T (AexT) in A. salmonicida subsp. salmonicida, the etiological agent of furunculosis in fish, was characterized. Gene aexT, encoding toxin AexT, was cloned and characterized by sequence analysis. AexT shows significant sequence similarity to the ExoS and ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.7.1851-1858.2002
更新日期:2002-04-01 00:00:00
abstract::Oligoribonuclease, an exoribonuclease specific for small oligoribonucleotides, was initially characterized 20 years ago (S. K. Niyogi and A. K. Datta, J. Biol. Chem. 250:7307-7312, 1975) and shown to be different from RNase II and polynucleotide phosphorylase. Here we demonstrate, using mutant strains and purified enz...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.14.4137-4139.1995
更新日期:1995-07-01 00:00:00
abstract::The production of extracellular alpha-amylase and protease by protoplasts of Bacillus amyloliquefaciens has been achieved. The production of enzymically active protease was totally dependent on a high concentration of either Mg2+, Ca2+, or spermidine, but production of active alpha-amylase was not. This cation depende...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.123.3.806-814.1975
更新日期:1975-09-01 00:00:00
abstract::Bacterial lysis induced by the expression of the cloned lysis gene of the RNA bacteriophage MS2 in Escherichia coli was shown to be under the same regulatory control mechanisms as penicillin-induced lysis. It was controlled by the stringent response and showed the phenomenon of tolerance when E. coli was grown at pH 5...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.11.5027-5033.1988
更新日期:1988-11-01 00:00:00
abstract::Despite a large number of studies on the role of oxygen in cellular processes, there is no consensus as to how oxygen availability to the cell should be defined, let alone how it should be quantified. Here, a quantitative definition for oxygen availability (perceived aerobiosis) is presented; the definition is based o...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.5.1402-1406.2002
更新日期:2002-03-01 00:00:00
abstract::A large-scale fractionation scheme purified the major molybdenum(Mo)-binding protein (Mop) from crude extracts of Clostridium pasteurianum, with a 10 and 0.2% yield of Mo and protein, respectively. The apparent molecular weight of the purified molybdoprotein is 5,700, as determined by sodium dodecyl sulfate-polyacryla...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.168.2.688-693.1986
更新日期:1986-11-01 00:00:00
abstract::Pasteurella multocida was grown in iron-free chemically defined medium supplemented with hemoglobin, transferrin, ferritin, and ferric citrate as iron sources. Whole-genome DNA microarrays were used to monitor global gene expression over seven time points after the addition of the defined iron source to the medium. Th...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.23.6714-6720.2002
更新日期:2002-12-01 00:00:00
abstract::The genes for the complete pathways for two polycyclic aromatic polyketides of the angucyclinone class have been cloned and heterologously expressed. Genomic DNAs of Streptomyces rimosus NRRL 3016 and Streptomyces strain WP 4669 were partially digested with MboI, and libraries (ca. 40-kb fragments) in Escherichia coli...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.2.470-476.1997
更新日期:1997-01-01 00:00:00
abstract::The region of the Rhizobium leguminosarum biovar viciae Sym plasmid pRL1JI, responsible for the production and secretion of a previously described 50-kilodalton protein (R. A. de Maagd, C. A. Wijffelman, E. Pees, and B. J. J. Lugtenberg, J. Bacteriol. 170:4424-4427, 1988), was cloned and its nucleotide sequence was de...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.12.6764-6770.1989
更新日期:1989-12-01 00:00:00
abstract::The sequences of the capsular biosynthetic (cps) loci of 90 serotypes of Streptococcus pneumoniae have recently been determined. Bioinformatic procedures were used to predict the general functions of 1,973 of the 1,999 gene products and to identify proteins within the same homology group, Pfam family, and CAZy glycosy...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00837-07
更新日期:2007-11-01 00:00:00