Tetracycline-inducible transfer of tetracycline resistance in Bacteroides fragilis in the absence of detectable plasmid DNA.

abstract：:Bacillus subtilis cwlD and dacB mutants produce spore peptidoglycan (PG) with increased cross-linking but with little change in spore core hydration compared to the wild type. A cwlD dacB double mutant produced spores with a two- to fourfold greater increase in PG cross-linking and novel muropeptides containing glycin...

journal_title：Journal of bacteriology

authors： Popham DL,Meador-Parton J,Costello CE,Setlow P

更新日期：1999-10-01 00:00:00

abstract：:NifA is the general transcriptional activator of nitrogen fixation genes in diazotrophic bacteria. In Rhizobium leguminosarum bv. viciae UPM791, the nifA gene is part of a gene cluster (orf71 orf79 fixW orf5 fixABCX nifAB) separated by 896 bp from an upstream and divergent truncated duplication of nifH (DeltanifH). Sy...

journal_title：Journal of bacteriology

authors： Martínez M,Palacios JM,Imperial J,Ruiz-Argüeso T

更新日期：2004-10-01 00:00:00

abstract：:Clostridium difficile is a gram-positive, spore-forming enteric anaerobe which can infect humans and a wide variety of animal species. Recently, the incidence and severity of human C. difficile infection has markedly increased. In this study, we evaluated the genomic content of 73 C. difficile strains isolated from hu...

journal_title：Journal of bacteriology

authors： Janvilisri T,Scaria J,Thompson AD,Nicholson A,Limbago BM,Arroyo LG,Songer JG,Gröhn YT,Chang YF

更新日期：2009-06-01 00:00:00

abstract：:The sequence (2,700 bp) between the aldH and pspF genes of Escherichia coli was determined. The pspF gene encodes a sigma54 transcriptional activator of the phage shock protein (psp) operon (pspA to pspE). Downstream of the pspF transcribed region are two open reading frames (ORFs), ordL and goaG, convergently oriente...

journal_title：Journal of bacteriology

authors： Jovanovic G,Model P

更新日期：1997-05-01 00:00:00

abstract：:Certain mutations in the C-terminal region of the Escherichia coli division inhibitor protein MinC cause loss of function of the division inhibitor by making MinC more sensitive to degradation by Lon protease, implying a possible role for the C-terminal region in regulating the stability and cellular concentration of ...

journal_title：Journal of bacteriology

authors： Sen M,Rothfield LI

更新日期：1998-01-01 00:00:00

abstract：:A comparison of the distribution of CO oxidation activity between soluble and particulate protein fractions obtained after disruption of CO-grown Rhodopseudomonas gelatinosa 1 by French pressure cell breakage and osmotic lysis of spheroplasts suggested that, in situ, the enzyme complex was associated with the cell mem...

journal_title：Journal of bacteriology

authors： Wakim BT,Uffen RL

更新日期：1983-01-01 00:00:00

abstract：:The replication order of markers was studied in Bacillus subtilis strains bearing the trpE26 mutation by the use of the density transfer technique. An important difference in this order was observed in comparison with that of strain 168 T-. All markers tested of a chromosome segment extending from trpD to ilvA replica...

journal_title：Journal of bacteriology

authors： Trowsdale J,Anagnostopoulos C

更新日期：1975-06-01 00:00:00

abstract：:Double-stranded, 1.9-kilobase-pair (kbp) DNA molecules were found in 18 strains representing three pathogenic races of Fusarium oxysporum f. sp. conglutinans. The DNA element (pFOXC1) from a race 1 strain and the DNA element (pFOXC2) from a race 2 strain were shown by restriction endonuclease mapping to be linear. pFO...

journal_title：Journal of bacteriology

authors： Kistler HC,Leong SA

更新日期：1986-08-01 00:00:00

abstract：:The ToxR protein of Vibrio cholerae is an integral membrane protein that coordinately regulates the expression of virulence genes required for successful infection. ToxR has been shown to bind directly to and activate transcription of the cholera toxin (ctx) promoter. Within the amino-terminal cytoplasmic region of To...

journal_title：Journal of bacteriology

authors： Ottemann KM,DiRita VJ,Mekalanos JJ

更新日期：1992-11-01 00:00:00

abstract：:A general, genetic technique for determining the direction of transcription for bacterial genes is presented. By comparing the phenotype of Mu-Mud1 dilysogens with the phenotype of deletion-containing derivatives, the direction of transcription for the gene containing Mud1 can be unambiguously determined. This method ...

journal_title：Journal of bacteriology

authors： MacNeil D

更新日期：1981-04-01 00:00:00

abstract：:A compound, isolated from the culture fluid of a nadC auxotroph of Escherichia coli grown in a minimal medium, supports the growth of both a nadA and a nadB mutant. This metabolite exhibits an ultraviolet light absorption spectrum and a mass spectrum, different from quinolinic acid. This compound may be the precursor ...

journal_title：Journal of bacteriology

authors： Chen JL,Tritz GJ

更新日期：1975-01-01 00:00:00

abstract：:The tar locus of Escherichia coli specifies one of the major species of methyl-accepting proteins involved in the chemotactic behavior of this organism. The physical and genetic organization of the tar region was investigated with a series of specialized lambda transducing phages and plasmid clones. The tar gene was m...

journal_title：Journal of bacteriology

authors： Slocum MK,Parkinson JS

更新日期：1983-08-01 00:00:00

abstract：:Transcription of the archaeal trkA gene homolog in Methanosarcina mazei S-6 was studied at the optimal growth temperature of 37 degrees C and after heat shock at 45 degrees C. Northern (RNA) blotting results (transcript size) and data from primer extension experiments to map the transcription initiation site indicate ...

journal_title：Journal of bacteriology

authors： Conway de Macario E,Macario AJ

更新日期：1995-11-01 00:00:00

abstract：:The proteome of Haemophilus influenzae strain Rd KW20 was analyzed by liquid chromatography (LC) coupled with ion trap tandem mass spectrometry (MS/MS). This approach does not require a gel electrophoresis step and provides a rapidly developed snapshot of the proteome. In order to gain insight into the central metabol...

journal_title：Journal of bacteriology

authors： Kolker E,Purvine S,Galperin MY,Stolyar S,Goodlett DR,Nesvizhskii AI,Keller A,Xie T,Eng JK,Yi E,Hood L,Picone AF,Cherny T,Tjaden BC,Siegel AF,Reilly TJ,Makarova KS,Palsson BO,Smith AL

更新日期：2003-08-01 00:00:00

abstract：:Anabaenopeptins (AP) are bioactive cyclic hexapeptides synthesized nonribosomally in cyanobacteria. APs are characterized by several conserved motifs, including the ureido bond, N-methylation in position 5, and d-Lys in position 2. All other positions of the AP molecule are variable, resulting in numerous structural v...

journal_title：Journal of bacteriology

authors： Christiansen G,Philmus B,Hemscheidt T,Kurmayer R

更新日期：2011-08-01 00:00:00

abstract：:Yersinia pestis, which causes bubonic and pneumonic plague, forms pigmented red colonies on Congo red (CR) dye agar. The hmsHFRS genes required for CR binding (Crb(+)) are genetically linked to virulence-associated genes encoding a siderophore uptake system. These genes are contained in a 102-kb chromosomal pgm locus ...

journal_title：Journal of bacteriology

authors： Hare JM,McDonough KA

更新日期：1999-08-01 00:00:00

abstract：:Cerulenin is a fungal mycotoxin that potently inhibits fatty acid synthesis by covalent modification of the active site thiol of the chain-elongation subtypes of beta-ketoacyl-acyl carrier protein (ACP) synthases. The Bacillus subtilis fabF (yjaY) gene (fabF(b)) encodes an enzyme that catalyzes the condensation of mal...

journal_title：Journal of bacteriology

authors： Schujman GE,Choi KH,Altabe S,Rock CO,de Mendoza D

更新日期：2001-05-01 00:00:00

abstract：:The levels of transfer ribonucleic acids (tRNAs) specific for 14 amino acids were almost identical in dormant spores and in spores germinated from 6 to 75 min. Germinated spore tRNAs specific for all amino acids tested were between 63 and 93% charged, and there was no significant change in this value from 6 to 75 min ...

journal_title：Journal of bacteriology

authors： Setlow P

更新日期：1974-06-01 00:00:00

abstract：:We report that transformation of Streptomyces lividans with cloned DNA of the SLP1 genetic element results in integration of the element at the same chromosomal locus (attB) normally occupied by SLP1 in its original host, Streptomyces coelicolor, and in S. lividans that has received SLP1 by mating. We constructed SLP1...

journal_title：Journal of bacteriology

authors： Omer CA,Stein D,Cohen SN

更新日期：1988-05-01 00:00:00

abstract：:Cell size at bud initiation shows the same relationship to growth rate in wild-type and petite yeast cells. The nutrient-modulated size control is related to growth rate rather than a change from fermentative to oxidative metabolism. ...

journal_title：Journal of bacteriology

authors： Bugeja VC,Carter BL

更新日期：1982-08-01 00:00:00

abstract：:Gram-negative Aggregatibacter actinomycetemcomitans can be distinguished (based on the promoter structure of the leukotoxin operon) into JP2 and non-JP2 genotypes, with the former found to be more pathogenic than the latter. Here we report the first complete genome sequence of a serotype b non-JP2 strain of A. actinom...

journal_title：Journal of bacteriology

authors： Chen C,Kittichotirat W,Chen W,Downey JS,Bumgarner R

更新日期：2012-04-01 00:00:00

abstract：:Several preparations of staphylococcal alpha-toxin and delta-lysin were studied in order to compare hemolytic activity with capacity to lyse bacterial protoplasts. delta-Lysin in relatively low concentration lysed protoplasts of Sarcina lutea, protoplasts of Streptococcus faecalis, and spheroplasts of Escherichia coli...

journal_title：Journal of bacteriology

authors： Bernheimer AW,Avigad LS,Grushoff P

更新日期：1968-08-01 00:00:00

abstract：:Linker insertion mutagenesis was used to modify the paracrystalline surface layer (S-layer) protein (RsaA) of the gram-negative bacterium Caulobacter crescentus. Eleven unique BamHI linker insertions in the cloned rsaA gene were identified; at the protein level, these linker insertions introduced 4 to 6 amino acids at...

journal_title：Journal of bacteriology

authors： Bingle WH,Nomellini JF,Smit J

更新日期：1997-02-01 00:00:00

abstract：:In recent work, we described the excision of a large genomic region from Enterococcus faecium D344R in which the sequence from "joint" regions suggested that excision resulted from the interaction of conjugative transposon Tn916 and the related mobile element Tn5386. In the present study, we examined the ability of in...

journal_title：Journal of bacteriology

authors： Rice LB,Carias LL,Hutton-Thomas R,Rudin S

更新日期：2007-05-01 00:00:00

abstract：:A cryptic promoter, designated P alpha, initiates transcription within the O(R) region of bacteriophage lambda. Transcription from P alpha proceeds in the direction of the cI repressor gene from sites 46 and 48 bp preceding the PRM transcription start site. P alpha is likely to compete with both PR and PRM for formati...

journal_title：Journal of bacteriology

authors： Woody ST,Fong RS,Gussin GN

更新日期：1993-09-01 00:00:00

abstract：:Strains of Bacillus thuringiensis such as B. thuringiensis subsp. israelensis (ONR-60A) and B. thuringiensis subsp. morrisoni (PG-14) pathogenic for mosquito larvae produce a complex parasporal body consisting of several protein endotoxins synthesized during sporulation that form an aggregate of crystalline inclusions...

journal_title：Journal of bacteriology

authors： Diaz-Mendoza M,Bideshi DK,Federici BA

更新日期：2012-03-01 00:00:00

abstract：:The aminophenol (AP) catabolic operon in Pseudomonas putida HS12 mineralizing nitrobenzene was found to contain all the enzymes responsible for the conversion of AP to pyruvate and acetyl coenzyme A via extradiol meta cleavage of 2-aminophenol. The sequence and functional analyses of the corresponding genes of the ope...

journal_title：Journal of bacteriology

authors： Park HS,Kim HS

更新日期：2001-09-01 00:00:00

abstract：:Melanin production is a major virulence factor for Cryptococcus neoformans, an organism causing life-threatening infections in an estimated 10% of AIDS patients. In order to characterize the events involved in melanin synthesis, an enzyme having diphenol oxidase activity was purified and its gene was cloned. The enzym...

journal_title：Journal of bacteriology

authors： Williamson PR

更新日期：1994-02-01 00:00:00

abstract：:Two nrdF genes, nrdF1 and nrdF2, encoding the small subunit (R2) of ribonucleotide reductase (RR) from Mycobacterium tuberculosis have 71% identity at the amino acid level and are both highly homologous with Salmonella typhimurium R2F. The calculated molecular masses of R2-1 and R2-2 are 36,588 (322 amino acids [aa]) ...

journal_title：Journal of bacteriology

authors： Yang F,Curran SC,Li LS,Avarbock D,Graf JD,Chua MM,Lu G,Salem J,Rubin H

更新日期：1997-10-01 00:00:00

abstract：:Pseudomonas aeruginosa is usually resistant to a wide variety of antibacterial agents, and it has been inferred, on the basis of indirect evidence, that this was due to the low permeability of its outer membrane. We determined the permeability of P. aeruginosa outer membrane directly, by measuring the rates of hydroly...

journal_title：Journal of bacteriology

authors： Yoshimura F,Nikaido H

更新日期：1982-11-01 00:00:00