Symbiotic autoregulation of nifA expression in Rhizobium leguminosarum bv. viciae.

Abstract:

:NifA is the general transcriptional activator of nitrogen fixation genes in diazotrophic bacteria. In Rhizobium leguminosarum bv. viciae UPM791, the nifA gene is part of a gene cluster (orf71 orf79 fixW orf5 fixABCX nifAB) separated by 896 bp from an upstream and divergent truncated duplication of nifH (DeltanifH). Symbiotic expression analysis of genomic nifA::lacZ fusions revealed that in strain UPM791 nifA is expressed mainly from a sigma54-dependent promoter (P(nifA1)) located upstream of orf71. This promoter contains canonical NifA upstream activating sequences located 91 bp from the transcription initiation site. The transcript initiated in P(nifA1) spans 5.1 kb and includes nifA and nifB genes. NifA from Klebsiella pneumoniae was able to activate transcription from P(nifA1) in a heterologous Escherichia coli system. In R. leguminosarum, the P(nifA1) promoter is essential for effective nitrogen fixation in symbiosis with peas. In its absence, partially efficient nitrogen-fixing nodules were produced, and the corresponding bacteroids exhibited only low levels of nifA gene expression. The basal level of nifA expression resulted from a promoter activity originating upstream of the fixX-nifA intergenic region and probably from an incomplete duplication of P(nifA1) located immediately upstream of fixA.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Martínez M,Palacios JM,Imperial J,Ruiz-Argüeso T

doi

10.1128/JB.186.19.6586-6594.2004

keywords:

subject

Has Abstract

pub_date

2004-10-01 00:00:00

pages

6586-94

issue

19

eissn

0021-9193

issn

1098-5530

pii

186/19/6586

journal_volume

186

pub_type

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