RelA inhibits Bacillus subtilis motility and chaining.

abstract：:The highly defective rho-15 mutant of Escherichia coli produces plasmid DNA that is 22% less negatively supercoiled than DNA from an isogenic wild-type strain (J. S. Fassler, G. F. Arnold, and I. Tessman, Mol. Gen. Genet. 204:424-429, 1986). We extended our measurements of plasmid superhelicity to additional rho mutan...

journal_title：Journal of bacteriology

authors： Arnold GF,Tessman I

更新日期：1988-09-01 00:00:00

abstract：:This paper describes the regulation of a transfer ribonucleic acid (tRNA) biosynthetic enzyme, the tRNA(m5U)methyltransferase (EC 2.1.1.35). This enzyme catalyzes the formation of 5-methyluridine (m5U, ribothymidine) in all tRNA chains of Escherichia coli. Partial deprivation of charged tRNAVal can be imposed by shift...

journal_title：Journal of bacteriology

authors： Ny T,Björk GR

更新日期：1977-05-01 00:00:00

abstract：:Lipopolysaccharide (LPS) was isolated from the unicellular blue-green bacterium Agmenellum quadruplicatum using the procedure of Westphal and Jann (1965). It was composed of a lipid A and polysaccharide region suggesting a similarity to other gram-negative LPSs. Chemical analyses demonstrated the presence of glucose, ...

journal_title：Journal of bacteriology

authors： Buttke TM,Ingram LO

更新日期：1975-12-01 00:00:00

abstract：:An alcohol dehydrogenase (ADH) from hyperthermophilic archaeon Thermococcus guaymasensis was purified to homogeneity and was found to be a homotetramer with a subunit size of 40 ± 1 kDa. The gene encoding the enzyme was cloned and sequenced; this gene had 1,095 bp, corresponding to 365 amino acids, and showed high seq...

journal_title：Journal of bacteriology

authors： Ying X,Ma K

更新日期：2011-06-01 00:00:00

abstract：:Phytoplasmas are plant-pathogenic bacteria that cause numerous diseases. This study shows a strong positive selection on the phytoplasma antigenic membrane protein (Amp). The ratio of nonsynonymous to synonymous substitutions was >1 with all the methods we tested. The clear positive selections imply an important biolo...

journal_title：Journal of bacteriology

authors： Kakizawa S,Oshima K,Jung HY,Suzuki S,Nishigawa H,Arashida R,Miyata S,Ugaki M,Kishino H,Namba S

更新日期：2006-05-01 00:00:00

abstract：:Freter, Rolf (Jefferson Medical College, Philadelphia, Pa.), and Atsushi Ozawa. Explanation for limitation of populations of Escherichia coli in broth cultures. J. Bacteriol. 86:904-910. 1963.-Veal Infusion Broth surrounding a cellophane bag containing a 24-hr culture of Escherichia coli was inhibitory to an inoculum ...

journal_title：Journal of bacteriology

authors： FRETER R,OZAWA A

更新日期：1963-11-01 00:00:00

abstract：:We used two-dimensional gel electrophoresis to quantitate the changes in rates of synthesis that follow phage lambda infection for 21 Escherichia coli proteins, including groE and dnaK proteins. Although total protein synthesis and the rates of synthesis of most individual E. coli proteins decreased after infection, s...

journal_title：Journal of bacteriology

authors： Drahos DJ,Hendrix RW

更新日期：1982-03-01 00:00:00

abstract：:The cell walls of a selected isolate of Staphylococcus aureus FDA 209P were observed undergoing progressive disintegration when exposed to lysostaphin (1 unit/ml) in 24% NaCl solution. Electron micrographs of ultrathin sections of test cells after exposure to lysostaphin for 2 min showed only superficial evidence of l...

journal_title：Journal of bacteriology

authors： Schuhardt VT,Huber TW,Pope LM

更新日期：1969-01-01 00:00:00

abstract：:Antibiotic resistance carried on R factors was transferred by conjugation from Escherichia coli B/r and Shigella flexneri 1a to Erwinia spp. Tetracycline resistance (TetR) carried on R factor R100 drd-56 was transferred from E. coli B/r to strains of Erwinia amylovora, E. aroideae, E. atroseptica, E. chrysanthemi, E. ...

journal_title：Journal of bacteriology

authors： Chatterjee AK,Starr MP

更新日期：1972-10-01 00:00:00

abstract：:Early log-phase cells of Mycoplasma gallisepticum A5969 were synchronized by holding in Eagle minimal essential medium (MEM) for 2 h. When transferred out of MEM into tryptose medium, the cells exhibited synchronous growth. Deoxyribonucleic acid (DNA) synthesis proceeded continuously during this growth but stopped dur...

journal_title：Journal of bacteriology

authors： Quinlan DC,Maniloff J

更新日期：1973-07-01 00:00:00

abstract：:A fusion constructed between the putative attenuator plus the first 219 nucleotides of the ermC (erythromycin resistance) structural gene and a 5' terminally deleted lacZ gene produced a moderate, basal level of beta-galactosidase which was increased by erythromycin addition. Another construction containing an intact ...

journal_title：Journal of bacteriology

authors： Kirsch DR,Lai MH

更新日期：1984-07-01 00:00:00

abstract：:A cyclic version of the Entner-Doudoroff pathway is used by Pseudomonas aeruginosa to metabolize carbohydrates. Genes encoding the enzymes that catabolize intracellular glucose to pyruvate and glyceraldehyde 3-phosphate are coordinately regulated, clustered at 39 min on the chromosome, and collectively form the hex re...

journal_title：Journal of bacteriology

authors： Hager PW,Calfee MW,Phibbs PV

更新日期：2000-07-01 00:00:00

abstract：:Type 1 pili of Escherichia coli are the prototype of the somatic class of pili found on many strains of bacteria. As a first step in the genetic analysis of type 1 piliation, an extensive series of nonpiliated derivatives of E. coli K-12 strain AW405, was characterized to produce attached or free pili when examined in...

journal_title：Journal of bacteriology

authors： Swaney LM,Liu YP,To CM,To CC,Ippen-Ihler K,Brinton CC Jr

更新日期：1977-04-01 00:00:00

abstract：:Here we have characterized the Rickettsia prowazekii RP534 protein, a homologue of the Pseudomonas aeruginosa ExoU phospholipase A (PLA) secreted cytotoxin. Our studies showed that purified recombinant RP534 PLA possessed the predicted PLA(2) and lyso-PLA(2) activities based on what has been published for P. aeruginos...

journal_title：Journal of bacteriology

authors： Housley NA,Winkler HH,Audia JP

更新日期：2011-09-01 00:00:00

abstract：:The Mg-2+-Sarkosyl crystals (M band) procedure was used to study the effect of ribonuclease (RNase) A on the association of Escherichia coli deoxyribonucleic acid (DNA) with membrane. Incubation of gently prepared cell extracts with RNase results in the release of DNA from membrane. This effect appears to result from ...

journal_title：Journal of bacteriology

authors： McIntosh MA,Earhart CF

更新日期：1975-05-01 00:00:00

abstract：:The region of the colicin E1 polypeptide that interacts with immunity protein has been localized to a 168-residue COOH-terminal peptide. This is the length of a proteolytically generated peptide fragment of colicin E1 against which imm+ function can be demonstrated in osmotically shocked cells. The role of particular ...

journal_title：Journal of bacteriology

authors： Bishop LJ,Bjes ES,Davidson VL,Cramer WA

更新日期：1985-10-01 00:00:00

abstract：:The response of Salmonella typhimurium to various forms of environmental stress was examined by using O'Farrell two-dimensional gel electrophoresis. Polypeptides (a total of 110) which quantitatively increased during various starvations, anaerobiosis, or heat shock were identified and cataloged in reference to a stand...

journal_title：Journal of bacteriology

authors： Spector MP,Aliabadi Z,Gonzalez T,Foster JW

更新日期：1986-10-01 00:00:00

abstract：:top mutations (formerly supX) eliminate DNA topoisomerase I activity and suppress the leu-500 promoter mutation in Salmonella typhimurium (K. M. Overbye, S. K. Basu, and P. Margolin, Cold Spring Harbor Symp. Quant. Biol. 47:785-791, 1983). Sublethal doses of coumermycin which reduce intracellular levels of supercoilin...

journal_title：Journal of bacteriology

authors： Pruss GJ,Drlica K

更新日期：1985-11-01 00:00:00

abstract：:Pseudomonas putida DOT-T1E is a solvent-resistant strain that is able to grow in the presence of high concentrations of toluene. We have cloned and sequenced the cti gene of this strain, which encodes the cis/trans isomerase, termed Cti, that catalyzes the cis-trans isomerization of esterified fatty acids in phospholi...

journal_title：Journal of bacteriology

authors： Junker F,Ramos JL

更新日期：1999-09-01 00:00:00

abstract：:The vir-type IV secretion system of Agrobacterium is assembled from 12 proteins encoded by the virB operon and virD4. VirB1 is one of the least-studied proteins encoded by the virB operon. Its N terminus is a lytic transglycosylase. The C-terminal third of the protein, VirB1*, is cleaved from VirB1 and secreted to the...

journal_title：Journal of bacteriology

authors： Zupan J,Hackworth CA,Aguilar J,Ward D,Zambryski P

更新日期：2007-09-01 00:00:00

abstract：:A mutant of Escherichia coli is described which is defective in the conversion of arginine to putrescine. The activity of the enzyme agmatine ureohydrolase is greatly reduced, whereas the activity of the other two enzymes of the pathway, the constitutive arginine decarboxylase and the inducible arginine decarboxylase,...

journal_title：Journal of bacteriology

authors： Hirshfield IN,Rosenfeld HJ,Leifer Z,Maas WK

更新日期：1970-03-01 00:00:00

abstract：:The major outer membrane protein of molecular weight 40,000 (the 40K protein) of a virulent isolate of Bordetella pertussis was purified to apparent homogeneity. The purified protein formed an oligomer band (of apparent molecular weight 90,000) on sodium dodecyl sulfate-polyacrylamide gels after solubilization at low ...

journal_title：Journal of bacteriology

authors： Armstrong SK,Parr TR Jr,Parker CD,Hancock RE

更新日期：1986-04-01 00:00:00

abstract：:Azotobacter vinelandii is a bacterium which undergoes a differentiation process leading to the formation of metabolically dormant cysts. During the encystment process, A. vinelandii produces alkylresorcinol lipids (ARs) that replace the membrane phospholipids and are also components of the layers covering the cyst. Th...

journal_title：Journal of bacteriology

authors： Romero Y,Moreno S,Guzmán J,Espín G,Segura D

更新日期：2013-04-01 00:00:00

abstract：:Aerobic gram-negative methylotrophs oxidize methanol to formaldehyde by using a methanol dehydrogenase that has pyrroloquinoline quinone (PQQ) as a prosthetic group. Seventy-two mutants which are unable to grow on methanol unless the growth medium is supplemented with PQQ have been isolated in the facultative methanol...

journal_title：Journal of bacteriology

authors： Morris CJ,Biville F,Turlin E,Lee E,Ellermann K,Fan WH,Ramamoorthi R,Springer AL,Lidstrom ME

更新日期：1994-03-01 00:00:00

abstract：:We have constructed a series of broad-host-range plasmids which use "visual screens" to detect promoter activity. These plasmids contain the pMB1 and pRO1600 origins of replication and are capable of replicating in a wide range of gram-negative bacteria. The genes encoding beta-galactosidase and alkaline phosphatase f...

journal_title：Journal of bacteriology

authors： Farinha MA,Kropinski AM

更新日期：1990-06-01 00:00:00

abstract：:Early stages of nodulation involve the exchange of signals between the bacterium and the host plant. Bacterial nodulation (nod) genes are required for Rhizobium spp. to synthesize lipooligosaccharide morphogens, termed Nod factors. The common nod genes encode enzymes that synthesize the factor core structure, which is...

journal_title：Journal of bacteriology

authors： Ehrhardt DW,Atkinson EM,Faull KF,Freedberg DI,Sutherlin DP,Armstrong R,Long SR

更新日期：1995-11-01 00:00:00

abstract：:Phosphorylation of a major 57-kilodalton protein substrate was observed in cell lysates of Spiroplasma melliferum BC3 incubated with [gamma-32P]ATP. Only serine phosphates have been isolated from the acid hydrolysate of the phosphorylated protein. The 57-kilodalton protein substrate was found, to a large extent, in th...

journal_title：Journal of bacteriology

authors： Platt MW,Reizer J,Rottem S

更新日期：1990-05-01 00:00:00

abstract：:The synthesis of proteins necessary for the respiratory reduction of nitrate to dinitrogen is induced in most denitrifying bacteria by a shift to anaerobiosis. A homolog of the fur gene, which encodes a redox-active transcriptional activator in Escherichia coli, was isolated from Pseudomonas stutzeri by using the anr ...

journal_title：Journal of bacteriology

authors： Cuypers H,Zumft WG

更新日期：1993-11-01 00:00:00

abstract：:Dehalogenases are key enzymes in the metabolism of halo-organic compounds. This paper describes a systematic approach to the isolation and molecular analysis of two families of bacterial alpha-halocarboxylic acid (alphaHA) dehalogenase genes, called group I and group II deh genes. The two families are evolutionarily u...

journal_title：Journal of bacteriology

authors： Hill KE,Marchesi JR,Weightman AJ

更新日期：1999-04-01 00:00:00

abstract：:Selection for chlorate resistance yields mol (formerly chl) mutants with defects in molybdenum cofactor synthesis. Complementation and genetic mapping analyses indicated that the Klebsiella pneumoniae mol genes are functionally homologous to those of Escherichia coli and occupy analogous genetic map positions. Hypoxan...

journal_title：Journal of bacteriology

authors： Garzón A,Li J,Flores A,Casadesus J,Stewart V

更新日期：1992-10-01 00:00:00