Molybdenum cofactor (chlorate-resistant) mutants of Klebsiella pneumoniae M5al can use hypoxanthine as the sole nitrogen source.

Abstract:

:Selection for chlorate resistance yields mol (formerly chl) mutants with defects in molybdenum cofactor synthesis. Complementation and genetic mapping analyses indicated that the Klebsiella pneumoniae mol genes are functionally homologous to those of Escherichia coli and occupy analogous genetic map positions. Hypoxanthine utilization in other organisms requires molybdenum cofactor as a component of xanthine dehydrogenase, and thus most chlorate-resistant mutants cannot use hypoxanthine as a sole source of nitrogen. Surprisingly, the K. pneumoniae mol mutants and the mol+ parent grew equally well with hypoxanthine as the sole nitrogen source, suggesting that K. pneumoniae has a molybdenum cofactor-independent pathway for hypoxanthine utilization.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Garzón A,Li J,Flores A,Casadesus J,Stewart V

doi

10.1128/jb.174.19.6298-6302.1992

keywords:

subject

Has Abstract

pub_date

1992-10-01 00:00:00

pages

6298-302

issue

19

eissn

0021-9193

issn

1098-5530

journal_volume

174

pub_type

杂志文章
  • Transposition of Tn7 occurs at a single site on the Caulobacter crescentus chromosome.

    abstract::Insertion of Tn7 in nine independently derived isolates occurred in a single small region of the Caulobacter crescentus chromosome, suggesting that Tn7 insertion occurs at or near a single site. The order of markers in this region of the Caulobacter chromosome was shown to be flaA-argG-Tn7. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.151.2.1056-1058.1982

    authors: Ely B

    更新日期:1982-08-01 00:00:00

  • Isolation and characterization of the Escherichia coli htrD gene, whose product is required for growth at high temperatures.

    abstract::Those genes in Escherichia coli defined by mutations which result in an inability to grow at high temperatures are designated htr, indicating a high temperature requirement. A new htr mutant of E. coli was isolated and characterized and is designated htrD. The htrD gene has been mapped to 19.3 min on the E. coli chrom...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.4.1240-1247.1992

    authors: Delaney JM,Ang D,Georgopoulos C

    更新日期:1992-02-01 00:00:00

  • A Rhodobacter capsulatus member of a universal permease family imports molybdate and other oxyanions.

    abstract::Molybdenum (Mo) is an important trace element that is toxic at high concentrations. To resolve the mechanisms underlying Mo toxicity, Rhodobacter capsulatus mutants tolerant to high Mo concentrations were isolated by random transposon Tn5 mutagenesis. The insertion sites of six independent isolates mapped within the s...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00742-10

    authors: Gisin J,Müller A,Pfänder Y,Leimkühler S,Narberhaus F,Masepohl B

    更新日期:2010-11-01 00:00:00

  • Complete genomic sequence of the lytic bacteriophage phiYeO3-12 of Yersinia enterocolitica serotype O:3.

    abstract::phiYeO3-12 is a T3-related lytic bacteriophage of Yersinia enterocolitica serotype O:3. The nucleotide sequence of the 39,600-bp linear double-stranded DNA (dsDNA) genome was determined. The phage genome has direct terminal repeats of 232 bp, a GC content of 50.6%, and 54 putative genes, which are all transcribed from...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.6.1928-1937.2001

    authors: Pajunen MI,Kiljunen SJ,Söderholm ME,Skurnik M

    更新日期:2001-03-01 00:00:00

  • Changes in Escherichia coli rRNA promoter activity correlate with changes in initiating nucleoside triphosphate and guanosine 5' diphosphate 3'-diphosphate concentrations after induction of feedback control of ribosome synthesis.

    abstract::rRNA synthesis is the rate-limiting step in ribosome synthesis in Escherichia coli. Its regulation has been described in terms of a negative-feedback control loop in which rRNA promoter activity responds to the amount of translation. The feedback nature of this control system was demonstrated previously by artificiall...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.185.20.6185-6191.2003

    authors: Schneider DA,Gourse RL

    更新日期:2003-10-01 00:00:00

  • Complete genome sequence of Lactobacillus plantarum JDM1.

    abstract::Lactobacillus plantarum is a lactic acid bacterium (LAB) species commonly used as a probiotic. We have sequenced the genome of Lactobacillus plantarum JDM1, which is a Chinese commercial LAB with several probiotic functions, using a GS 20 system. We recommend that each commercial probiotic strain should undergo comple...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00587-09

    authors: Zhang ZY,Liu C,Zhu YZ,Zhong Y,Zhu YQ,Zheng HJ,Zhao GP,Wang SY,Guo XK

    更新日期:2009-08-01 00:00:00

  • Whole-genome sequence of Streptococcus pseudopneumoniae isolate IS7493.

    abstract::Streptococcus pseudopneumoniae is a member of the viridans group streptococci (VGS) whose pathogenic significance is unclear. We announce the complete genome sequence of S. pseudopneumoniae IS7493. The genome sequence will assist in the characterization of this new organism and facilitate the development of accurate d...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.06075-11

    authors: Shahinas D,Tamber GS,Arya G,Wong A,Lau R,Jamieson F,Ma JH,Alexander DC,Low DE,Pillai DR

    更新日期:2011-11-01 00:00:00

  • Photosynthetic sulfide oxidation by Chloroflexus aurantiacus, a filamentous, photosynthetic, gliding bacterium.

    abstract::Chloroflexus, a newly described genus of filamentous, photosynthetic, gliding bacteria, oxidizes sulfide anaerobically under photoautotrophic or photoheterotrophic growh conditions and deposits elemental sulfur outside the cell. The formation of sulfur granules outside the cell supports the idea that this organism is ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.122.2.782-784.1975

    authors: Madigan MT,Brock TD

    更新日期:1975-05-01 00:00:00

  • A third beta-galactosidase in a strain of Klebsiella that possesses two lac genes.

    abstract::Klebsiella strain RE1544 contains two lac operons, one on the chromosome and one on a lac plasmid. A mutant of RE1544, in which the lacZ genes of both operons produce no active enzyme, was found to synthesize a beta-galactosidase that hydrolyzes ortho-nitrophenyl-beta-D-galactopyranoside but not lactose. Synthesis of ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.132.1.219-223.1977

    authors: Hall BG,Reeve EC

    更新日期:1977-10-01 00:00:00

  • Structural model for 12-helix transporters belonging to the major facilitator superfamily.

    abstract::The major facilitator superfamily includes a large collection of evolutionarily related proteins that have been implicated in the transport of a variety of solutes and metabolites across the membranes of organisms ranging from bacteria to humans. We have recently reported the three-dimensional structure, at 6.5 A reso...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.185.5.1712-1718.2003

    authors: Hirai T,Heymann JA,Maloney PC,Subramaniam S

    更新日期:2003-03-01 00:00:00

  • Nutritional requirements for vegetative growth of Myxococcus xanthus.

    abstract::Dworkin, Martin (Indiana University Medical Center, Indianapolis, Ind.). Nutritional requirements for vegetative growth of Myxococcus xanthus. J. Bacteriol. 84:250-257. 1962.-This investigation was part of a program to clarify the environmental regulation of fruiting-body formation in a fruiting myxobacterium. By use ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.84.2.250-257.1962

    authors: DWORKIN M

    更新日期:1962-08-01 00:00:00

  • Isolation of Neurospora crassa bradytrophs.

    abstract::A method was developed for the isolation of Neurospora bradytrophs. The bradytrophs (representing lesions in a number of pathways) were resistant to DL-p-fluorophenylalanine when growing in a leaky fashion but were sensitive when grown in the presence of their stimulating supplement. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.140.3.1133-1136.1979

    authors: Kinsey JA

    更新日期:1979-12-01 00:00:00

  • Identification of a global repressor gene, rsmA, of Erwinia carotovora subsp. carotovora that controls extracellular enzymes, N-(3-oxohexanoyl)-L-homoserine lactone, and pathogenicity in soft-rotting Erwinia spp.

    abstract::The production of extracellular enzymes such as pectate lyase (Pel), polygalacturonase (Peh), cellulase (Cel), and protease (Prt) is activated by the cell density (quorum)-sensing signal, N-(3-oxohexanoyl)-L-homoserine lactone (HSL); plant signals; and aep genes during postexponential growth of Erwinia carotovora subs...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.17.5108-5115.1995

    authors: Cui Y,Chatterjee A,Liu Y,Dumenyo CK,Chatterjee AK

    更新日期:1995-09-01 00:00:00

  • The Bacillus subtilis signaling protein SpoIVB defines a new family of serine peptidases.

    abstract::The protein SpoIVB plays a key role in signaling in the final sigma(K) checkpoint of Bacillus subtilis. This regulatory mechanism coordinates late gene expression during development in this organism and we have recently shown SpoIVB to be a serine peptidase. SpoIVB signals by transiting a membrane, undergoing self-cle...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.184.1.191-199.2002

    authors: Hoa NT,Brannigan JA,Cutting SM

    更新日期:2002-01-01 00:00:00

  • Efficiency of thymidine incorporation in Escherichia coli B-r as a function of growth rate.

    abstract::Short-term labeling with radioactive thymidine led to inaccurate estimates of deoxyribonucleic acid synthesis at some growth rates in steady-state cultures of Escherichia coli B/r. Estimates were corrected in chemostat cultures by adding adenosine, a known inhibitor of thymidine phosphorylase. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.110.3.1208-1210.1972

    authors: Kubitschek HE,Valdez D,Freedman ML

    更新日期:1972-06-01 00:00:00

  • Antipain lethality to Escherichia coli: dependence upon cyclic adenosine 3',5'-monophosphate and its receptor protein.

    abstract::Antipain kills Escherichia coli K-12 cells in an exponential manner beginning 1 h after its addition. Mutant strains, delta cya and crp, which are unable to synthesize cyclic adenosine 3',5'-monophosphate (cAMP) and the cAMP receptor protein, respectively, are not affected. Addition of cAMP (5 mM) to antipain-treated ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.139.2.690-693.1979

    authors: Swenson PA

    更新日期:1979-08-01 00:00:00

  • Electron transport in the pathway of acetate conversion to methane in the marine archaeon Methanosarcina acetivorans.

    abstract::A liquid chromatography-hybrid linear ion trap-Fourier transform ion cyclotron resonance mass spectrometry approach was used to determine the differential abundance of proteins in acetate-grown cells compared to that of proteins in methanol-grown cells of the marine isolate Methanosarcina acetivorans metabolically lab...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.188.2.702-710.2006

    authors: Li Q,Li L,Rejtar T,Lessner DJ,Karger BL,Ferry JG

    更新日期:2006-01-01 00:00:00

  • Transport of 5-aminolevulinic acid by the dipeptide permease in Salmonella typhimurium.

    abstract::In a previous search for mutants of Salmonella typhimurium that are defective in heme synthesis, one class that is apparently defective in 5-aminolevulinic acid (ALA) uptake (alu) was found. Here, I describe the characterization of these mutations. The mutations all map to a single locus near 77.5 min on the genetic m...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.2.325-331.1993

    authors: Elliott T

    更新日期:1993-01-01 00:00:00

  • Promoters of the CATG-specific methyltransferase gene hpyIM differ between iceA1 and iceA2 Helicobacter pylori strains.

    abstract::Helicobacter pylori strains can be divided into two groups, based on the presence of two unrelated genes, iceA1 and iceA2, that occupy the same genomic locus. hpyIM, located immediately downstream of either gene, encodes a functional CATG-specific methyltransferase. Despite the strong conservation of the hpyIM open re...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.13.3875-3884.2001

    authors: Xu Q,Blaser MJ

    更新日期:2001-07-01 00:00:00

  • The dimerization function of MinC resides in a structurally autonomous C-terminal domain.

    abstract::Limited proteolysis of the Escherichia coli cell division inhibitor MinC reveals that its dimerization function resides in a structurally autonomous C-terminal domain. We show that cytoplasmic MinC is poised near the monomer-dimer equilibrium and propose that it only becomes entirely dimeric once recruited to the memb...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.22.6684-6687.2001

    authors: Szeto TH,Rowland SL,King GF

    更新日期:2001-11-01 00:00:00

  • Isolation and characterization of a selenium metabolism mutant of Salmonella typhimurium.

    abstract::Selenium is a constituent in Escherichia coli of the anaerobic enzyme formate dehydrogenase in the form of selenocysteine. Selenium is also present in the tRNA of E. coli in the modified base 5-methylaminomethyl-2-selenouracil (mnm5Se2U). The pathways of bacterial selenium metabolism are largely uncharacterized, and i...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.170.2.736-743.1988

    authors: Kramer GF,Ames BN

    更新日期:1988-02-01 00:00:00

  • Bacteriophage Mu-induced modification of DNA is dependent upon a host function.

    abstract::The DNA of bacteriophage Mu, extracted from induced lysates, is partially resistant to digestion by the endonuclease BalI. This modification of DNA is controlled by the Mu modification function (mom), which acts in conjunction with the dam (DNA-adenine methylation) function of Escherichia coli. Since the BalI recognit...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.136.1.423-428.1978

    authors: Khatoon H,Bukhari AI

    更新日期:1978-10-01 00:00:00

  • Gene cluster of Pseudomonas syringae pv. "phaseolicola" controls pathogenicity of bean plants and hypersensitivity of nonhost plants.

    abstract::Loss of the ability of Pseudomonas syringae pv. "phaseolicola" NPS3121 to elicit a hypersensitive response on tobacco and other nonhost plants was associated with loss of pathogenicity on the susceptible host bean. Eight independent, prototrophic transposon Tn5 insertion mutants which had lost the ability to elicit a ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.168.2.512-522.1986

    authors: Lindgren PB,Peet RC,Panopoulos NJ

    更新日期:1986-11-01 00:00:00

  • Platinum-induced filamentous growth in Escherichia coli.

    abstract::Certain group VIIIB transition metal compounds were found to inhibit cell division in Escherichia coli, causing marked filamentous growth. Gram-negative bacilli were the most sensitive to this effect, whereas gram-positive bacilli responded only at near-toxic levels of the metal. None of the cocci tested showed any ap...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.93.2.716-721.1967

    authors: Rosenberg B,Renshaw E,Vancamp L,Hartwick J,Drobnik J

    更新日期:1967-02-01 00:00:00

  • Characterization of the F-plasmid conjugative transfer gene traU.

    abstract::We characterized the traU gene of the Escherichia coli K-12 conjugative plasmid F. Plasmids carrying segments of the F transfer operon were tested for their capacity to complement F lac traU526. The protein products of TraU+ clones were identified, and the nucleotide sequence of traU was determined. traU mapped betwee...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.8.4263-4270.1990

    authors: Moore D,Maneewannakul K,Maneewannakul S,Wu JH,Ippen-Ihler K,Bradley DE

    更新日期:1990-08-01 00:00:00

  • Novel xylose dehydrogenase in the halophilic archaeon Haloarcula marismortui.

    abstract::During growth of the halophilic archaeon Haloarcula marismortui on D-xylose, a specific D-xylose dehydrogenase was induced. The enzyme was purified to homogeneity. It constitutes a homotetramer of about 175 kDa and catalyzed the oxidation of xylose with both NADP+ and NAD+ as cosubstrates with 10-fold higher affinity ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.186.18.6198-6207.2004

    authors: Johnsen U,Schönheit P

    更新日期:2004-09-01 00:00:00

  • Influences of various amino acids on tryptophan-mediated control of the tryptophan biosynthetic enzymes in Escherichia coli.

    abstract::Lysates of Escherichia coli Ymel obtained from cultures grown in the absence of tryptophan in minimal medium supplemented with 0.1% casein hydrolysate show an approximate fivefold increase in steady-state specific activity of both anthranilate synthetase and tryptophan synthetase A protein relative to cultures grown i...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.108.3.1181-1191.1971

    authors: Stubbs JD,Stubbs EA

    更新日期:1971-12-01 00:00:00

  • Quantitative measurements of proton motive force and motility in Bacillus subtilis.

    abstract::The protein motive force of metabolizing Bacillus subtilis cells was only slightly affected by changes in the external pH between 5 and 8, although the electrical component and the chemical component of the proton motive force contributed differently at different external pH. The electrical component of the proton mot...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.144.3.891-897.1980

    authors: Shioi JI,Matsuura S,Imae Y

    更新日期:1980-12-01 00:00:00

  • Transcript analysis of multiple copies of amo (encoding ammonia monooxygenase) and hao (encoding hydroxylamine oxidoreductase) in Nitrosomonas europaea.

    abstract::The genes encoding ammonia monooxygenase (amoCAB), hydroxylamine oxidoreductase (hao), and the c-type cytochrome c-554 (hcy) are present in multiple copies in the genome of Nitrosomonas europaea. The upstream regions of the two copies of amoC, the three copies of hao, and one copy of hcy were cloned and sequenced. Pri...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.3.1096-1100.2001

    authors: Hommes NG,Sayavedra-Soto LA,Arp DJ

    更新日期:2001-02-01 00:00:00

  • phs Locus of Escherichia coli, a mutation causing pleiotropic lesions in metabolism, is an rpoA allele.

    abstract::The phs mutation, which causes a pleiotropic growth defect, has been mapped and shown to be an allele of rpoA, the gene for the alpha subunit of RNA polymerase. The mutation is shown to cause a transcription defect in the arabinose operon, araBAD. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.164.2.972-975.1985

    authors: Rowland GC,Giffard PM,Booth IR

    更新日期:1985-11-01 00:00:00