Abstract:
:We characterized the traU gene of the Escherichia coli K-12 conjugative plasmid F. Plasmids carrying segments of the F transfer operon were tested for their capacity to complement F lac traU526. The protein products of TraU+ clones were identified, and the nucleotide sequence of traU was determined. traU mapped between traW and trbC. It encodes a 330-amino-acid, Mr36,786 polypeptide that is processed. Ethanol caused accumulation of a precursor polypeptide; removal of ethanol permitted processing of the protein to occur. Because F lac traU526 strains appear to be resistant to F-pilus-specific phages, traU has been considered an F-pilus assembly gene. However, electron microscopic analysis indicated that the traU526 amber mutation caused only a 50% reduction in F-piliation. Since F lac traU526 strains also retain considerable transfer proficiency, new traU mutations were constructed by replacing a segment of traU with a kanamycin resistance gene. Introduction of these mutations into a transfer-proficient plasmid caused a drastic reduction in transfer proficiency, but pilus filaments remained visible at approximately 20% of the wild-type frequency. Like traU526 strains, such mutants were unable to plaque F-pilus-specific phages but exhibited a slight sensitivity on spot tests. Complementation with a TraU+ plasmid restored the wild-type transfer and phage sensitivity phenotypes. Thus, an intact traU product appears to be more essential to conjugal DNA transfer than to assembly of pilus filaments.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Moore D,Maneewannakul K,Maneewannakul S,Wu JH,Ippen-Ihler K,Bradley DEdoi
10.1128/jb.172.8.4263-4270.1990subject
Has Abstractpub_date
1990-08-01 00:00:00pages
4263-70issue
8eissn
0021-9193issn
1098-5530journal_volume
172pub_type
杂志文章abstract::Methods were devised to purify the cell-associated, iron-binding compounds known as mycobactins from the closely related species Mycobacterium avium, Mycobacterium intracellulare, and Mycobacterium scrofulaceum (i.e., the MAIS complex of organisms). The mycobactins from these three species showed a structure that is c...
journal_title:Journal of bacteriology
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journal_title:Journal of bacteriology
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doi:10.1128/JB.94.3.691-699.1967
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:1992-08-01 00:00:00
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更新日期:1982-09-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2008-07-01 00:00:00
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pub_type: 杂志文章
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更新日期:1996-06-01 00:00:00
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更新日期:1992-01-01 00:00:00
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更新日期:2003-05-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.137.3.1386-1394.1979
更新日期:1979-03-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1996-01-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.1.593-595.1989
更新日期:1989-01-01 00:00:00
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journal_title:Journal of bacteriology
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doi:10.1128/JB.95.1.43-46.1968
更新日期:1968-01-01 00:00:00
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journal_title:Journal of bacteriology
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更新日期:2011-09-01 00:00:00
abstract::The current work by Jain et al. (S. Jain, A. M. Chang, M. Singh, J. S. McLean, et al., J Bacteriol 201:e00683-18, 2019, https://doi.org/10.1128/JB.00683-18) reports the cloning of the lipid A deacylase gene of Porphyromonas gingivalis and the phenotypic characterization of the enzyme. Attempts to clone the gene and th...
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pub_type: 评论,杂志文章
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更新日期:2019-05-08 00:00:00
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pub_type: 杂志文章
doi:10.1128/jb.175.3.779-784.1993
更新日期:1993-02-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.19.5579-5585.1996
更新日期:1996-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.159.1.199-205.1984
更新日期:1984-07-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.16.5286-5288.1993
更新日期:1993-08-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/jb.182.13.3870-3873.2000
更新日期:2000-07-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.133.2.492-498.1978
更新日期:1978-02-01 00:00:00
abstract::A method was devised for the isolation of yeast monomorphic (LEV) mutants of Candida albicans. By this procedure, about 20 stable yeast-like mutants were isolated after mutagenesis with ethyl methane sulfonate. The growth rate of the mutants in different carbon sources, both fermentable and not, was indistinguishable ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.8.2318-2325.1994
更新日期:1994-04-01 00:00:00
abstract::Cells of Pseudomonas stutzeri are naturally transformed by homologous chromosomal DNA; they do not require chemical treatment to become competent. This capacity to undergo natural transformation was found to be shared by the closely related species P. mendocina, P. alcaligenes, and P. pseudoalcaligenes, but was not de...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.153.1.93-99.1983
更新日期:1983-01-01 00:00:00
abstract::Niederpruem, Donald J. (Indiana University Medical Center, Indianapolis). Respiration of basidiospores of Schizophyllum commune. J. Bacteriol. 88:210-215. 1964.-The aerobic metabolism of basidiospores of the wood-rotting mushroom Schizophyllum commune was investigated by use of manometric techniques and specific respi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.88.1.210-215.1964
更新日期:1964-07-01 00:00:00