Thermostable chemotaxis proteins from the hyperthermophilic bacterium Thermotoga maritima.

Abstract:

:An expressed sequence tag homologous to cheA was previously isolated by random sequencing of Thermotoga maritima cDNA clones (C. W. Kim, P. Markiewicz, J. J. Lee, C. F. Schierle, and J. H. Miller, J. Mol. Biol. 231: 960-981, 1993). Oligonucleotides complementary to this sequence tag were synthesized and used to identify a clone from a T. maritima lambda library by using PCR. Two partially overlapping restriction fragments were subcloned from the lambda clone and sequenced. The resulting 5,251-bp sequence contained five open reading frames, including cheA, cheW, and cheY. In addition to the chemotaxis genes, the fragment also encodes a putative protein isoaspartyl methyltransferase and an open reading frame of unknown function. Both the cheW and cheY genes were individually cloned into inducible Escherichia coli expression vectors. Upon induction, both proteins were synthesized at high levels. T. maritima CheW and CheY were both soluble and were easily purified from the bulk of the endogenous E. coli protein by heat treatment at 80 degrees C for 10 min. CheY prepared in this way was shown to be active by the demonstration of Mg(2+)-dependent autophosphorylation with [32P]acetyl phosphate. In E. coli, CheW mediates the physical coupling of the receptors to the kinase CheA. The availability of a thermostable homolog of CheW opens the possibility of structural characterization of this small coupling protein, which is among the least well characterized proteins in the bacterial chemotaxis signal transduction pathway.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Swanson RV,Sanna MG,Simon MI

doi

10.1128/jb.178.2.484-489.1996

subject

Has Abstract

pub_date

1996-01-01 00:00:00

pages

484-9

issue

2

eissn

0021-9193

issn

1098-5530

journal_volume

178

pub_type

杂志文章
  • New antibiotic substance produced by Salmonella typhimurium LT2.

    abstract::Salmonella typhimurium LT2 excreted under certain conditions an antibiotic substance designated typhimuricin. It is suggested that the LT2 "cryptic" plasmid is involved in its production and in the immunity to it. Preliminary characterization of typhimuricin is presented. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:

    authors: Ben-Gurion R,Flashner Y

    更新日期:1982-10-01 00:00:00

  • Involvement of a cytochrome P450 monooxygenase in thaxtomin A biosynthesis by Streptomyces acidiscabies.

    abstract::The biosynthesis of the thaxtomin cyclic dipeptide phytotoxins proceeds nonribosomally via the thiotemplate mechanism. Acyladenylation, thioesterification, N-methylation, and cyclization of two amino acid substrates are catalyzed by the txtAB-encoded thaxtomin synthetase. Nucleotide sequence analysis of the region 3' ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.184.7.2019-2029.2002

    authors: Healy FG,Krasnoff SB,Wach M,Gibson DM,Loria R

    更新日期:2002-04-01 00:00:00

  • Mutagenesis of Neisseria gonorrhoeae: absence of error-prone repair.

    abstract::The lethal and mutagenic effects of various mutagens on Neisseria gonorrhoeae were investigated. Lethality studies demonstrated that N. gonorrhoeae was relatively sensitive to ethyl methanesulfonate, UV light, and methyl methanesulfonate. Although N. gonorrhoeae was readily mutated by ethyl methanesulfonate and N-meth...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.160.1.288-293.1984

    authors: Campbell LA,Yasbin RE

    更新日期:1984-10-01 00:00:00

  • Discontinuity and limited linkage in the homologous recombination system of a hyperthermophilic archaeon.

    abstract::Genetic transformation of Sulfolobus acidocaldarius by a multiply marked pyrE gene provided a high-resolution assay of homologous recombination in a hyperthermophilic archaeon. Analysis of 100 Pyr(+) transformants revealed that this recombination system could transfer each of 23 nonselected base pair substitutions to ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00447-10

    authors: Grogan DW,Rockwood J

    更新日期:2010-09-01 00:00:00

  • Immunofluorescence of green monkey kidney cells infected with adenovirus 12 and with adenovirus 12 plus simian virus 40.

    abstract::Malmgren, Richard A. (National Cancer Institute; Bethesda, Md.), Alan S. Rabson, Paula G. Carney, and Frances J. Paul. Immunofluorescence of green monkey kidney cells infected with adenovirus 12 and with adenovirus 12 plus simian virus 40. J. Bacteriol. 91:262-265. 1966.-Immunofluorescence studies of the viral antigen...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.91.1.262-265.1966

    authors: Malmgren RA,Rabson AS,Carney PG,Paul FJ

    更新日期:1966-01-01 00:00:00

  • COLORADO TICK FEVER VIRUS IN CELL CULTURE. I. CELL-TYPE SUSCEPTIBILITY AND INTERACTION WITH L CELLS.

    abstract::Trent, Dennis W. (University of Oklahoma School of Medicine, Oklahoma City) and L. Vernon Scott. Colorado tick fever virus in cell culture. I. Cell-type susceptibility and interaction with L cells. J. Bacteriol. 88:702-708. 1964.-Colorado tick fever (CTF) virus was serially propagated in monolayer cultures of L and FL...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.88.3.702-708.1964

    authors: TRENT DW,SCOTT LV

    更新日期:1964-09-01 00:00:00

  • Escherichia coli outer membrane protein K is a porin.

    abstract::Protein K is an outer membrane protein found in pathogenic encapsulated strains of Escherichia coli. We present evidence here that protein K is structurally and functionally related to the E. coli K-12 porin proteins (OmpF, OmpC, and PhoE). Protein K was found to cross-react with antibody to OmpF protein and to share ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.156.2.867-872.1983

    authors: Sutcliffe J,Blumenthal R,Walter A,Foulds J

    更新日期:1983-11-01 00:00:00

  • Quorum sensing in Vibrio fischeri: evidence that S-adenosylmethionine is the amino acid substrate for autoinducer synthesis.

    abstract::Synthesis of the autoinducer signal involved in the cell density-dependent activation of Vibrio fischeri luminescence is directed by luxI. The autoinducer is N-(3-oxohexanoyl)homoserine lactone, and little is known about its synthesis. We have measured autoinducer synthesis by amino acid auxotrophs of Escherichia coli...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.17.5291-5294.1996

    authors: Hanzelka BL,Greenberg EP

    更新日期:1996-09-01 00:00:00

  • Localization of P42 and F(1)-ATPase α-subunit homolog of the gliding machinery in Mycoplasma mobile revealed by newly developed gene manipulation and fluorescent protein tagging.

    abstract::Mycoplasma mobile has a unique mechanism that enables it to glide on solid surfaces faster than any other gliding mycoplasma. To elucidate the gliding mechanism, we developed a transformation system for M. mobile based on a transposon derived from Tn4001. Modification of the electroporation conditions, outgrowth time,...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01418-13

    authors: Tulum I,Yabe M,Uenoyama A,Miyata M

    更新日期:2014-05-01 00:00:00

  • Genomic organization and molecular characterization of SM1, a temperate bacteriophage of Streptococcus mitis.

    abstract::The direct binding of Streptococcus mitis to human platelets is mediated in part by two proteins (PblA and PblB) encoded by a lysogenic bacteriophage (SM1). Since SM1 is the first prophage of S. mitis that has been identified and because of the possible role of these phage-encoded proteins in virulence, we sought to c...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.185.23.6968-6975.2003

    authors: Siboo IR,Bensing BA,Sullam PM

    更新日期:2003-12-01 00:00:00

  • Novel toluene elimination system in a toluene-tolerant microorganism.

    abstract::In studies of Pseudomonas putida IH-2000, a toluene-tolerant microorganism, membrane vesicles (MVs) were found to be released from the outer membrane when toluene was added to the culture. These MVs were found to be composed of phospholipids, lipopolysaccharides (LPS), and very low amounts of outer membrane proteins. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.182.22.6451-6455.2000

    authors: Kobayashi H,Uematsu K,Hirayama H,Horikoshi K

    更新日期:2000-11-01 00:00:00

  • Organization and functions of genes in the upstream region of tyrT of Escherichia coli: phenotypes of mutants with partial deletion of a new gene (tgs).

    abstract::A delta tyrT::kan mutant from Escherichia coli K-12 (DTK-12) shows a transient growth lag that is caused by glycine starvation (U. Michelsen, M. Bösl, T. Dingermann, and H. Kersten, J. Bacteriol. 171:5987-5994, 1989). The same deletion, transduced into the relA1 spoT1 mutant CA274 to construct strain DTC274, caused co...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.1.221-231.1994

    authors: Bösl M,Kersten H

    更新日期:1994-01-01 00:00:00

  • Occurrence of D-rhamnan as the common antigen reactive against monoclonal antibody E87 in Pseudomonas aeruginosa IFO 3080 and other strains.

    abstract::S. Sawada and co-workers reported that a monoclonal antibody (MAb), E87, interacted with about 80% of Pseudomonas aeruginosa isolates, and they separated a rhamnose-rich polysaccharide as the probable antigen for MAb E87 from P. aeruginosa IFO 3080 (S. Sawada, T. Kawamura, Y. Masuho, and K. Tomibe, J. Infec. Dis. 152:...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.10.6162-6164.1990

    authors: Yokota S,Kaya S,Araki Y,Ito E,Kawamura T,Sawada S

    更新日期:1990-10-01 00:00:00

  • A Bacillus cereus cytolytic determinant, cereolysin AB, which comprises the phospholipase C and sphingomyelinase genes: nucleotide sequence and genetic linkage.

    abstract::A cloned cytolytic determinant from the genome of Bacillus cereus GP-4 has been characterized at the molecular level. Nucleotide sequence determination revealed the presence of two open reading frames. Both open reading frames were found by deletion and complementation analysis to be necessary for expression of the he...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.2.744-753.1989

    authors: Gilmore MS,Cruz-Rodz AL,Leimeister-Wächter M,Kreft J,Goebel W

    更新日期:1989-02-01 00:00:00

  • Surface viscoelasticity of individual gram-negative bacterial cells measured using atomic force microscopy.

    abstract::The cell envelope of gram-negative bacteria is responsible for many important biological functions: it plays a structural role, it accommodates the selective transfer of material across the cell wall, it undergoes changes made necessary by growth and division, and it transfers information about the environment into th...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00132-08

    authors: Vadillo-Rodriguez V,Beveridge TJ,Dutcher JR

    更新日期:2008-06-01 00:00:00

  • Lysine catabolism in Streptomyces spp. is primarily through cadaverine: beta-lactam producers also make alpha-aminoadipate.

    abstract::Genetic and biochemical evidence was obtained for lysine catabolism via cadaverine and delta-aminovalerate in both the beta-lactam producer Streptomyces clavuligerus and the nonproducer Streptomyces lividans. This pathway is used when lysine is supplied as the sole source of nitrogen for the organism. A second pathway...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.1.299-302.1989

    authors: Madduri K,Stuttard C,Vining LC

    更新日期:1989-01-01 00:00:00

  • Extracellular hemolysins of aerobic sporogenic bacilli.

    abstract::Forty-five strains, representing 18 species of the genus Bacillus, were surveyed for production of hemolysin against rabbit erythrocytes. Broth cultures of B. cereus, B. alvei, and B. laterosporus contained lysins that closely resembled streptolysin O. B. subtilis and a single strain of B. cereus may produce lysins ha...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.93.5.1541-1543.1967

    authors: Bernheimer AW,Grushoff P

    更新日期:1967-05-01 00:00:00

  • Massetolide A biosynthesis in Pseudomonas fluorescens.

    abstract::Massetolide A is a cyclic lipopeptide (CLP) antibiotic produced by various Pseudomonas strains from diverse environments. Cloning, sequencing, site-directed mutagenesis, and complementation showed that massetolide A biosynthesis in P. fluorescens SS101 is governed by three nonribosomal peptide synthetase (NRPS) genes,...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01563-07

    authors: de Bruijn I,de Kock MJ,de Waard P,van Beek TA,Raaijmakers JM

    更新日期:2008-04-01 00:00:00

  • Sequence diversity of Treponema pallidum subsp. pallidum tprK in human syphilis lesions and rabbit-propagated isolates.

    abstract::The tprK gene of Treponema pallidum subsp. pallidum, the causative agent of venereal syphilis, belongs to a 12-member gene family and encodes a protein with a predicted cleavable signal sequence and predicted transmembrane domains. Except for the Nichols type strain, all rabbit-propagated isolates of T. pallidum exami...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.185.21.6262-6268.2003

    authors: LaFond RE,Centurion-Lara A,Godornes C,Rompalo AM,Van Voorhis WC,Lukehart SA

    更新日期:2003-11-01 00:00:00

  • Life cycle of Neurospora crassa viewed by scanning electron microscopy.

    abstract::Scanning electron microscopy was used to examine the major stages of the life cycle of two wild-type strains of Neurospora crassa Shear and Dodge (St. Lawrence 3.1a and 74A): mycelia, protoperithecium formation, perithecia, ascospores, ascospore germination and outgrowth, macro and microconidia, and germination and ou...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.113.2.1015-1025.1973

    authors: Seale T

    更新日期:1973-02-01 00:00:00

  • Adhesive activity of the haemophilus cryptic genospecies cha autotransporter is modulated by variation in tandem Peptide repeats.

    abstract::The Haemophilus cryptic genospecies is an important cause of maternal genital tract and neonatal systemic infections and initiates infection by colonizing the genital or respiratory epithelium. In recent work, we identified a unique Haemophilus cryptic genospecies protein called Cha, which mediates efficient adherence...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00933-10

    authors: Sheets AJ,St Geme JW 3rd

    更新日期:2011-01-01 00:00:00

  • Physiology of lysine permeases in Saccharomycopsis lipolytica.

    abstract::Two active lysine transport systems were detected in Saccharomycopsis lipolytica. No excretion of lysine out of the cells could be obtained, even by chasing with L-lysine or by poisoning with sodium azide. The kinetic properties of one of the permeases, the high-affinity lysine permease, were studied in detail. Its Km...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.133.2.492-498.1978

    authors: Beckerich JM,Heslot H

    更新日期:1978-02-01 00:00:00

  • Construction of broad-host-range plasmid vectors for easy visible selection and analysis of promoters.

    abstract::We have constructed a series of broad-host-range plasmids which use "visual screens" to detect promoter activity. These plasmids contain the pMB1 and pRO1600 origins of replication and are capable of replicating in a wide range of gram-negative bacteria. The genes encoding beta-galactosidase and alkaline phosphatase f...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.6.3496-3499.1990

    authors: Farinha MA,Kropinski AM

    更新日期:1990-06-01 00:00:00

  • Identification of repressor binding sites controlling expression of tetracycline resistance encoded by Tn10.

    abstract::The regulatory region controlling the expression of tetracycline resistance and repressor genes contains two nearly identical regions of dyad symmetry. Deletions of this control region were isolated by digestion with S1 nuclease. The ability of these deletions to bind the tet repressor was determined by an in vivo rep...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.156.3.1188-1191.1983

    authors: Wray LV Jr,Reznikoff WS

    更新日期:1983-12-01 00:00:00

  • Outer membrane proteins and cell surface structure of Selenomonas ruminantium.

    abstract::The protein compositions of the membrane preparations from Selenomonas ruminantium grown in glucose or lactate medium were determined by sodium dodecyl sulfate- and two-dimensional (first, isoelectric focusing; second, sodium dodecyl sulfate) polyacrylamide slab gel electrophoresis. The outer membrane from both glucos...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.141.2.899-907.1980

    authors: Kamio Y,Takahashi H

    更新日期:1980-02-01 00:00:00

  • Regulation and function of cytochrome c' in Rhodobacter sphaeroides 2.4.3.

    abstract::Cytochrome c' (Cyt c') is a c-type cytochrome with a pentacoordinate heme iron. The gene encoding this protein in Rhodobacter sphaeroides 2.4.3, designated cycP, was isolated and sequenced. Northern blot analysis and beta-galactosidase assays demonstrated that cycP transcription increased as oxygen levels decreased an...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.187.12.4077-4085.2005

    authors: Choi PS,Grigoryants VM,Abruña HD,Scholes CP,Shapleigh JP

    更新日期:2005-06-01 00:00:00

  • Mutants suppressing novobiocin hypersensitivity of a mukB null mutation.

    abstract::The mukB gene is essential for the partitioning of sister chromosomes in Escherichia coli. A mukB null mutant is hypersensitive to the DNA gyrase inhibitor novobiocin. In this work, we isolated mutants suppressing the novobiocin hypersensitivity of the mukB null mutation. All suppressor mutations are localized in or n...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.185.13.3690-3695.2003

    authors: Adachi S,Hiraga S

    更新日期:2003-07-01 00:00:00

  • Secretion of nuclease across the outer membrane of Serratia marcescens and its energy requirements.

    abstract::Extracellular secretion of Serratia marcescens nuclease occurs as a two-step process via a periplasmic intermediate. Unlike other extracellular proteins secreted by gram-negative bacteria by the general secretory pathway, nuclease accumulates in the periplasm in its active form for an unusually long time before its ex...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.3.677-683.1997

    authors: Suh Y,Benedik MJ

    更新日期:1997-02-01 00:00:00

  • Preparations and properties of ribonucleic acid polymerase from Acinetobacter calcoaceticus.

    abstract::Deoxyribonucleic acid (DNA)-dependent ribonucleic acid (RNA) polymerase (EC 2.7.7.6) from Acinetobacter calcoaceticus was purified to apparent homogeneity and its properties were compared with those of the Escherichia coli B enzyme. The molecular weights of the two native active enzymes as well as their alpha and beta...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.125.2.435-443.1976

    authors: Kleppe RK,Kleppe K

    更新日期:1976-02-01 00:00:00

  • Characterization of two tetracycline resistance determinants in Streptococcus faecalis JH1.

    abstract::Streptococcus faecalis strain JH1 harbors two conjugative plasmids: pJH1, an R plasmid mediating resistance to kanamycin, streptomycin, gentamicin, erythromycin, and tetracycline, and pJH2, a hemolysin-bacteriocin plasmid. Studies of plasmid-cured derivatives of strain JH1 and of transconjugates obtained after mixed i...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.150.2.835-843.1982

    authors: LeBlanc DJ,Lee LN

    更新日期:1982-05-01 00:00:00