A Bacillus cereus cytolytic determinant, cereolysin AB, which comprises the phospholipase C and sphingomyelinase genes: nucleotide sequence and genetic linkage.

Abstract:

:A cloned cytolytic determinant from the genome of Bacillus cereus GP-4 has been characterized at the molecular level. Nucleotide sequence determination revealed the presence of two open reading frames. Both open reading frames were found by deletion and complementation analysis to be necessary for expression of the hemolytic phenotype by Bacillus subtilis and Escherichia coli hosts. The 5' open reading frame was found to be nearly identical to a recently reported phospholipase C gene derived from a mutant B. cereus strain which overexpresses the respective protein, and it conferred a lecithinase-positive phenotype to the B. subtilis host. The 3' open reading frame encoded a sphingomyelinase. The two tandemly encoded activities, phospholipase C and sphingomyelinase, constitute a biologically functional cytolytic determinant of B. cereus termed cereolysin AB.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Gilmore MS,Cruz-Rodz AL,Leimeister-Wächter M,Kreft J,Goebel W

doi

10.1128/jb.171.2.744-753.1989

subject

Has Abstract

pub_date

1989-02-01 00:00:00

pages

744-53

issue

2

eissn

0021-9193

issn

1098-5530

journal_volume

171

pub_type

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