Abstract:
:A cloned cytolytic determinant from the genome of Bacillus cereus GP-4 has been characterized at the molecular level. Nucleotide sequence determination revealed the presence of two open reading frames. Both open reading frames were found by deletion and complementation analysis to be necessary for expression of the hemolytic phenotype by Bacillus subtilis and Escherichia coli hosts. The 5' open reading frame was found to be nearly identical to a recently reported phospholipase C gene derived from a mutant B. cereus strain which overexpresses the respective protein, and it conferred a lecithinase-positive phenotype to the B. subtilis host. The 3' open reading frame encoded a sphingomyelinase. The two tandemly encoded activities, phospholipase C and sphingomyelinase, constitute a biologically functional cytolytic determinant of B. cereus termed cereolysin AB.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Gilmore MS,Cruz-Rodz AL,Leimeister-Wächter M,Kreft J,Goebel Wdoi
10.1128/jb.171.2.744-753.1989subject
Has Abstractpub_date
1989-02-01 00:00:00pages
744-53issue
2eissn
0021-9193issn
1098-5530journal_volume
171pub_type
杂志文章abstract::ZoBell, Claude E. (University of California, La Jolla) and Andre B. Cobet. Growth, reproduction, and death rates of Escherichia coli at increased hydrostatic pressures. J. Bacteriol. 84:1228-1236. 1962.-Pressures ranging from 100 to 500 atm were found to retard the growth and reproduction of Escherichia coli in nutrie...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.84.6.1228-1236.1962
更新日期:1962-12-01 00:00:00
abstract:UNLABELLED:The ubiquitous bacterial second messenger cyclic di-GMP (c-di-GMP) has recently become prominent as a trigger for biofilm formation in many bacteria. It is generated by diguanylate cyclases (DGCs; with GGDEF domains) and degraded by specific phosphodiesterases (PDEs; containing either EAL or HD-GYP domains)....
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00520-15
更新日期:2015-08-24 00:00:00
abstract::The genome sequence analysis of a clinical Vibrio cholerae VC35 strain from an outbreak case in Malaysia indicates multiple genes involved in host adaptation and a novel Na(+)-driven multidrug efflux pump-coding gene in the genome of Vibrio cholerae with the highest similarity to VMA_001754 of Vibrio mimicus VMA223. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01832-12
更新日期:2012-12-01 00:00:00
abstract::Cultural characteristics and serological relationships of pathogenic marine vibrios isolated from fish in the Pacific Northwest were studied. These organisms were compared with cultures of Vibrio anguillarum, a known fish pathogen. On the basis of morphological and cultural characteristics, the Pacific Northwest strai...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.100.3.1242-1247.1969
更新日期:1969-12-01 00:00:00
abstract::The genetic requirements for the excision repair of thymine glycols, urea residues, and apurinic (AP) sites were examined by measuring the survival in Escherichia coli mutants of phi X174 replicative form (RF) I transfecting DNA containing selectively introduced lesions. phi X RF I DNA containing thymine glycols was i...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.8.3359-3366.1988
更新日期:1988-08-01 00:00:00
abstract::The reduction of bisulfite by Desulfovibrio vulgaris was investigated. Crude extracts reduced bisulfite to sulfide without the formation (detection) of any intermediates such as trithionate or thiosulfate. When the particulate fractions was removed from crude extracts by high-speed centrifugation, the soluble supernat...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.136.3.916-923.1978
更新日期:1978-12-01 00:00:00
abstract::The genetics of antibiotic resistance in mutant strains of Streptococcus pyrogenes was studied. Utilizing a type 6 strain (9440) primarily resistant to strepttomycin (Strr), classes of mutant strains were isolated that were resistant to one of the following antibiotics: rifampin (Rifr), erythromycin (Eryr), thiostrept...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.133.2.852-859.1978
更新日期:1978-02-01 00:00:00
abstract::The expression of any given bacterial protein is predicted to depend on (i) the transcriptional regulation of the promoter and the translational regulation of its mRNA and (ii) the synthesis and translation of total (bulk) mRNA. This is because total mRNA acts as a competitor to the specific mRNA for the binding of in...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.11.3037-3044.2000
更新日期:2000-06-01 00:00:00
abstract::Hall, Herbert E. (Robert A. Taft Sanitary Engineering Center, Cincinnati, Ohio), Robert Angelotti, Keith H. Lewis, and Milton J. Foter. Characteristics of Clostridium perfringens strains associated with food and food-borne disease. J. Bacteriol. 85:1094-1103. 1963.-A total of 83 strains of Clostridium perfringens-30 f...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.85.5.1094-1103.1963
更新日期:1963-05-01 00:00:00
abstract::Well-characterized Salmonella mutants formerly used in biosynthetic studies of lipopolysaccharides were used to study the toxic portion of the complex endotoxin. Endotoxins prepared from wild types and their mutants were tested for their biological activities, including pyrogenicity, lethality, and immunogenicity. The...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.94.5.1320-1326.1967
更新日期:1967-11-01 00:00:00
abstract::Biofilms contribute to virulence of Staphylococcus aureus. Formation of biofilms is multifactorial, involving polysaccharide, protein, and DNA components, which are controlled by various regulators. Here we report that deletion of the rsp gene resulted in an increase in biofilm formation in strain MW2, suggesting that...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.05454-11
更新日期:2011-10-01 00:00:00
abstract::When 100 group A streptococcus isolates were screened, drs, a variant of sic, was identified in emm12 and emm55 isolates. Molecular characterization showed that the drs gene sequence is highly conserved, unlike the sic gene sequence. However, the variation in gene size observed was due to the presence of extra interna...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01619-06
更新日期:2007-04-01 00:00:00
abstract::Despite the considerable knowledge of bacterial high-molecular-weight (HMW) polycyclic aromatic hydrocarbon (PAH) metabolism, the key enzyme(s) and its pleiotropic and epistatic behavior(s) responsible for low-molecular-weight (LMW) PAHs in HMW PAH-metabolic networks remain poorly understood. In this study, a phenotyp...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01945-14
更新日期:2014-10-01 00:00:00
abstract::The prokaryotic transposable element Tn5 has been found to promote the formation of adjacent deletions. The frequency of adjacent deletion formation is much lower than that of normal transposition events. Like normal transposition, however, adjacent deletion formation requires the activity of the transposase protein. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.5.1264-1271.1993
更新日期:1993-03-01 00:00:00
abstract::The chromosomally mediated penicillinase present in three strains of Escherichia coli K-12 has been purified and characterized. Two of the strains carried the ampA gene and the third the wild-type allele. The purification involves release of the enzyme by spheroplast formation, dialysis, chromatography on sulfoethyl c...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.101.1.218-231.1970
更新日期:1970-01-01 00:00:00
abstract::The mutB gene of Salmonella typhimurium is involved in a methylation-independent repair pathway specific for A/G or A/C mismatches and is the homolog of the Escherichia coli mutY gene. The mutB gene of S. typhimurium was cloned and sequenced. The isolated mutB clone reduced the mutation rate of the mutB mutant to wild...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.2.541-543.1993
更新日期:1993-01-01 00:00:00
abstract::Listeria monocytogenes has caused numerous human outbreaks. Here we report draft genomes of L. monocytogenes J1816 and J1-220, which belong to epidemic clones II and IV, respectively. Whole-genome sequence analysis of these strains provides a tool for studying the short-term evolution of these epidemic clones. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.05048-11
更新日期:2011-07-01 00:00:00
abstract::The pattern of transcription of the rplKAJLrpoBC gene cluster of Escherichia coli appears to be complex. At least four different promoters and a transcriptional attenuator have been identified. To compare the relative effect of each of the putative promoters and the attenuator on transcription of these genes, we fused...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.158.1.279-285.1984
更新日期:1984-04-01 00:00:00
abstract::DNA helicase I, encoded on the Escherichia coli F plasmid, catalyzes a site- and strand-specific nicking reaction within the F plasmid origin of transfer (oriT) to initiate conjugative DNA strand transfer. The product of the nicking reaction contains a single phosphodiester bond interruption as determined by single-nu...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.9.2599-2606.1993
更新日期:1993-05-01 00:00:00
abstract::Sinorhizobium meliloti can live as a soil saprophyte and can engage in a nitrogen-fixing symbiosis with plant roots. To succeed in such diverse environments, the bacteria must continually adjust gene expression. Transcriptional plasticity in eubacteria is often mediated by alternative sigma (σ) factors interacting wit...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00449-12
更新日期:2012-09-01 00:00:00
abstract::It previously has been proposed that the Escherichia coli SecB protein promotes the export of the maltose-binding protein (MBP) from the cytoplasm by preventing the folding of the precursor MBP (preMBP) into a translocation-incompetent conformation. The export of wild-type MBP is only partially blocked in SecB- cells....
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.9.4640-4647.1989
更新日期:1989-09-01 00:00:00
abstract::The fate of 35-S during anaerobic metabolism of [35-S]sulfate, [35-S]thiosulfate, and [35-S]sulfate plus unlabeled thiosulfate by washed cell suspensions of Desulfovibrio spp, and of [35-S]thiosulfate by growing D. desulfuricans was examined. The results appear to be inconsistent with the hypothesis that thiosulfate i...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.123.1.36-40.1975
更新日期:1975-07-01 00:00:00
abstract::Agrobacterium radiobacter NCIB 11883 was grown in lactose-limited continuous culture at a dilution rate of 0.045/h. Washed cells transported [14C]lactose and [methyl-14C]beta-D-thiogalactoside, a nonmetabolisable analog of lactose, at similar rates and with similar affinities (Km for transport, less than 1 microM). Tr...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.4.1703-1710.1990
更新日期:1990-04-01 00:00:00
abstract::The amounts of glycogen and trehalose have been measured in cells of a prototrophic diploid yeast strain subjected to a variety of nutrient limitations. Both glycogen and trehalose were accumulated in cells deprived specifically of nirogen, sulfur, or phosphorus, suggesting that reserve carbohydrate accumulation is a ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.143.3.1384-1394.1980
更新日期:1980-09-01 00:00:00
abstract::Transcriptional expression of the puc operon in Rhodobacter sphaeroides 2.4.1 is dependent on the partial pressure of oxygen. By using transcriptional fusions in trans of a promoterless fragment derived from the aminoglycoside-3'-phosphotransferase gene of Tn903 to puc operon-specific DNA containing a 629-bp 5' cis-ac...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.4.1158-1171.1992
更新日期:1992-02-01 00:00:00
abstract::The DNAs of strains of three cyanobacterial genera (Anabaena, Plectonema, and Synechococcus) were found to be partially or fully resistant to many restriction endonucleases. This could be due to the absence of specific sequences or to modifications, rendering given sequences resistant to cleavage. The latter explanati...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.4.1934-1939.1988
更新日期:1988-04-01 00:00:00
abstract::One of the most puzzling results from the complete genome sequence of the methanogenic archaeon Methanococcus jannaschii was that the organism may have only one DNA polymerase gene. This is because no other DNA polymerase-like open reading frames (ORFs) were found besides one ORF having the typical alpha-like DNA poly...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.8.2232-2236.1998
更新日期:1998-04-01 00:00:00
abstract::It previously has been shown that lkyD mutants of Salmonella typhimurium form large blebs of outer membrane over the septal and polar regions of dividing cells. To determine whether the outer membrane blebs are formed over potential sites of division even in the absence of septal ingrowth, lkyD strains were studied un...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.143.2.1019-1024.1980
更新日期:1980-08-01 00:00:00
abstract::A systematic analysis of the type III secretion (T3S) genes of Pseudomonas aeruginosa strain PAO1 revealed that they are under quorum-sensing control. This observation was supported by the down-regulation of the T3S regulon in the presence of RhlR-C4HSL and the corresponding advanced secretion of ExoS in a rhlI mutant...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.11.3898-3902.2005
更新日期:2005-06-01 00:00:00
abstract::Although yeast cannot normally incorporate exogenous deoxythymidine 5'-monophosphate (dTMP) into deoxyribonucleic acid, mutants able to do so have been isolated. We have characterized a recessive suppressor of dTMP uptake (sot1) that prevents strains carrying either tup1, tup2, or tup4 from growing on selective medium...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.138.2.638-641.1979
更新日期:1979-05-01 00:00:00