A novel DNA polymerase family found in Archaea.

Abstract:

:One of the most puzzling results from the complete genome sequence of the methanogenic archaeon Methanococcus jannaschii was that the organism may have only one DNA polymerase gene. This is because no other DNA polymerase-like open reading frames (ORFs) were found besides one ORF having the typical alpha-like DNA polymerase (family B). Recently, we identified the genes of DNA polymerase II (the second DNA polymerase) from the hyperthermophilic archaeon Pyrococcus furiosus, which has also at least one alpha-like DNA polymerase (T. Uemori, Y. Sato, I. Kato, H. Doi, and Y. Ishino, Genes Cells 2:499-512, 1997). The genes in M. jannaschii encoding the proteins that are homologous to the DNA polymerase II of P. furiosus have been located and cloned. The gene products of M. jannaschii expressed in Escherichia coli had both DNA polymerizing and 3'-->5' exonuclease activities. We propose here a novel DNA polymerase family which is entirely different from other hitherto-described DNA polymerases.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Ishino Y,Komori K,Cann IK,Koga Y

doi

10.1128/JB.180.8.2232-2236.1998

subject

Has Abstract

pub_date

1998-04-01 00:00:00

pages

2232-6

issue

8

eissn

0021-9193

issn

1098-5530

journal_volume

180

pub_type

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