Abstract:
:One of the most puzzling results from the complete genome sequence of the methanogenic archaeon Methanococcus jannaschii was that the organism may have only one DNA polymerase gene. This is because no other DNA polymerase-like open reading frames (ORFs) were found besides one ORF having the typical alpha-like DNA polymerase (family B). Recently, we identified the genes of DNA polymerase II (the second DNA polymerase) from the hyperthermophilic archaeon Pyrococcus furiosus, which has also at least one alpha-like DNA polymerase (T. Uemori, Y. Sato, I. Kato, H. Doi, and Y. Ishino, Genes Cells 2:499-512, 1997). The genes in M. jannaschii encoding the proteins that are homologous to the DNA polymerase II of P. furiosus have been located and cloned. The gene products of M. jannaschii expressed in Escherichia coli had both DNA polymerizing and 3'-->5' exonuclease activities. We propose here a novel DNA polymerase family which is entirely different from other hitherto-described DNA polymerases.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Ishino Y,Komori K,Cann IK,Koga Ydoi
10.1128/JB.180.8.2232-2236.1998subject
Has Abstractpub_date
1998-04-01 00:00:00pages
2232-6issue
8eissn
0021-9193issn
1098-5530journal_volume
180pub_type
杂志文章abstract::Screening of derivatives of Rhizobium etli KIM5s randomly mutagenized with mTn5SSgusA30 resulted in the identification of strain KIM-G1. Its rough colony appearance, flocculation in liquid culture, and Ndv(-) Fix(-) phenotype were indicative of a lipopolysaccharide (LPS) defect. Electrophoretic analysis of cell-associ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.18.5606-5614.1999
更新日期:1999-09-01 00:00:00
abstract::Membrane fractions from a lon strain of Escherichia coli but not a wild-type strain catalyze the incorporation of fucose from guanosine 5'-diphosphate-fucose into a lipid and into polymeric material. Both incorporation reactions specifically require only uridine 5'-diphosphate (UDP)-glucose. The sugar lipid was shown ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.129.1.225-236.1977
更新日期:1977-01-01 00:00:00
abstract::Twenty-four-hour-old, aerobically grown, Luria-Bertani broth cultures of Salmonella typhimurium F98 suppressed the growth of a spectinomycin-resistant (Spcr) derivative of the same strain inoculated at 10(3) CFU ml(-1). This growth suppression is genus specific and RpoS independent, and it is not solely a result of nu...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.22.7186-7190.1997
更新日期:1997-11-01 00:00:00
abstract::Sbarra, Anthony J. (St. Margaret's Hospital, Boston, Mass.), William Shirley, and John S. Baumstark. Effect of osmolarity on phagocytosis. J. Bacteriol. 85:306-313. 1963.-The effect of a number of different compounds on phagocytosis was studied. Phagocytosis was monitored by morphological and biochemical means. It was...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.85.2.306-313.1963
更新日期:1963-02-01 00:00:00
abstract::Herbaspirillum sp. strain GW103 was isolated from rhizosphere soil of the reed Phragmites australis on reclaimed land. Here we report the 5.05-Mb draft genome sequence of the strain, providing bioinformation about the agronomic benefits of this strain, such as multiple traits relevant to plant root colonization and pl...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00806-12
更新日期:2012-08-01 00:00:00
abstract::Radiorespirometric and enzymatic analyses reveal that glucose-grown cells of Rhizobium japonicum isolates I-110 and L1-110, both derivatives of R. japonicum strain 3I1b110, possess an active tricarboxylic acid cycle and metabolize glucose by simultaneous operation of the Embden-Meyerhof-Parnas and Entner-Doudoroff pat...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.131.1.179-187.1977
更新日期:1977-07-01 00:00:00
abstract::Vibrio vulnificus is an estuarine bacterium and potent opportunistic human pathogen. It enters the food chain by asymptomatically colonizing a variety of marine organisms, most notably oysters. Expression of the brp-encoded extracellular polysaccharide, which enhances cell-surface adherence, is regulated by cyclic di-...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00344-17
更新日期:2017-09-05 00:00:00
abstract::The genetic organization of the polypeptides required for the biosynthesis of the F41 adhesin of enterotoxigenic Escherichia coli strains was investigated. Maxicell analysis demonstrated that a recombinant plasmid which mediated mannose-resistant hemagglutination and F41 antigen production encoded four polypeptides of...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.10.4890-4896.1988
更新日期:1988-10-01 00:00:00
abstract::The region upstream of the Escherichia coli bgl operon is an insertion hot spot for several transposons. Elements as distantly related as Tn1, Tn5, and phage Mu home in on this location. To see what characteristics result in a high-affinity site for transposition, we compared in vivo and in vitro Mu transposition patt...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.11.3328-3335.2001
更新日期:2001-06-01 00:00:00
abstract::Agrobacterium radiobacter NCIB 11883 was grown in lactose-limited continuous culture at a dilution rate of 0.045/h. Washed cells transported [14C]lactose and [methyl-14C]beta-D-thiogalactoside, a nonmetabolisable analog of lactose, at similar rates and with similar affinities (Km for transport, less than 1 microM). Tr...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.4.1703-1710.1990
更新日期:1990-04-01 00:00:00
abstract::Some strains of Streptomyces produce sialidases. Two sialidases were purified over 1,000-fold from a culture filtrate of two Streptomyces species. They had the same properties in molecular weight, behavior to ions and other reagents, and substrate specificity. They showed very small differences in kinetic properties, ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.119.2.394-400.1974
更新日期:1974-08-01 00:00:00
abstract::The nylon oligomer (6-aminohexanoic acid cyclic dimer) degradation genes on plasmid pOAD2 of Flavobacterium sp. KI72 were cloned into Escherichia coli vector pBR322. The locus of one of the genes, the structural gene of 6-aminohexanoic acid linear oligomer hydrolase, was determined by constructing various deletion pla...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.155.1.22-31.1983
更新日期:1983-07-01 00:00:00
abstract::In Pseudomonas aeruginosa, flagellar genes are regulated in a cascade headed by FleQ, an NtrC/NifA-type activator. FleQ and RpoN positively regulate expression of flhA, fliE, fliL, and fleSR genes, among others. Direct interaction of FleQ with flhA, fliE, fliL, and fleSR promoters was demonstrated by gel shift assay, ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.19.5251-5260.2002
更新日期:2002-10-01 00:00:00
abstract::A modified transformation procedure that is effective for the introduction of plasmid deoxyribonucleic acid at high frequency into Salmonella typhimurium, as well as into Escherichia coli, is described. Transformed bacteria acquire a circular deoxyribonucleic acid species having the genetic and molecular characteristi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.119.3.1072-1074.1974
更新日期:1974-09-01 00:00:00
abstract::Burkholderia cepacia AC1100 metabolizes 2,4,5-trichlorophenoxyacetic acid (2,4,5-T) via formation of 5-chlorohydroxyquinol (5-CHQ), hydroxyquinol (HQ), maleylacetate, and beta-oxoadipate. The step(s) leading to the dechlorination of 5-CHQ to HQ has remained unidentified. We demonstrate that a dechlorinating enzyme, Tf...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.17.4667-4675.1998
更新日期:1998-09-01 00:00:00
abstract::We show that hisT function is required for normal growth of Escherichia coli K-12, since a lack of hisT-mediated pseudouridine tRNA modification causes a uracil requirement that interferes with cell division. We also show that hisT transcription is positively growth rate regulated in exponentially growing bacteria and...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.22.7395-7400.1991
更新日期:1991-11-01 00:00:00
abstract::A novel phenotype is described for Escherichia coli K-12 carrying the prlA4 allele determining a membrane component of the protein export mechanism. It is manifest as transformation deficiency for plasmids containing the cloned group C streptococcal streptokinase gene, skc. Streptokinase plasmid mutations relieving th...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.4.2202-2208.1989
更新日期:1989-04-01 00:00:00
abstract::We developed a procedure for isolating membrane vesicles from the homolactic fermentative bacterium Streptococcus cremoris. The membrane vesicles were shown to have a right-side-out orientation by freeze-etch electron microscopy and to be free of cytoplasmic constituents. The membrane vesicles retained their functiona...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.149.2.733-738.1982
更新日期:1982-02-01 00:00:00
abstract::Expression of the hurIR bhuRSTUV heme utilization locus in Bordetella bronchiseptica is coordinately controlled by the global iron-dependent regulator Fur and the extracytoplasmic function sigma factor HurI. Activation of HurI requires transduction of a heme-dependent signal via HurI, HurR, and BhuR, a three-component...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00629-07
更新日期:2007-09-01 00:00:00
abstract::A strain of Veillonella parvula M4, which grows readily in lactate broth without a requirement for carbon dioxide, has been isolated from the oral cavity. Anaerobic, washed cells of this organism fermented sodium lactate to the following products (moles/100 moles of lactate): propionate, 66; acetate, 40; carbon dioxid...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.105.3.999-1005.1971
更新日期:1971-03-01 00:00:00
abstract::Temperature-sensitive nalA mutants of Escherichia coli have been used to investigate the structure and functions of deoxyribonucleic acid (DNA) gyrase. Extracts of one such mutant (nalA43) had thermosensitive DNA gyrase subunit A activity but normal gyrase subunit B activity, proving definitively that nalA is the stru...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.140.2.424-435.1979
更新日期:1979-11-01 00:00:00
abstract::The aquatic bacterium Caulobacter crescentus attaches to solid surfaces through an adhesive holdfast located at the tip of its polar stalk, a thin cylindrical extension of the cell membrane. In this paper, the elastic properties of the C. crescentus stalk and holdfast assembly were studied by using video light microsc...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.1.257-265.2005
更新日期:2005-01-01 00:00:00
abstract::An extremely thermophilic bacterium, Thermus thermophilus HB8, is one of the model organisms for systems biology. Its genome consists of a chromosome (1.85 Mb), a megaplasmid (0.26 Mb) designated pTT27, and a plasmid (9.3 kb) designated pTT8, and the complete sequence is available. We show here that T. thermophilus is...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00662-10
更新日期:2010-10-01 00:00:00
abstract::Strains of Bacillus thuringiensis such as B. thuringiensis subsp. israelensis (ONR-60A) and B. thuringiensis subsp. morrisoni (PG-14) pathogenic for mosquito larvae produce a complex parasporal body consisting of several protein endotoxins synthesized during sporulation that form an aggregate of crystalline inclusions...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.06095-11
更新日期:2012-03-01 00:00:00
abstract::Bacillus subtilis, which grows under aerobic conditions, employs fatty acid desaturase (Des) to fluidize its membrane when subjected to temperature downshift. Des requires molecular oxygen for its activity, and its expression is regulated by DesK-DesR, a two-component system. Transcription of des is induced by the tem...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00384-10
更新日期:2010-08-01 00:00:00
abstract::In both prokaryotes and eukaryotes, hydroxyurea is suggested to inhibit DNA replication by inactivating ribonucleotide reductase and depleting deoxyribonucleoside triphosphate pools. In this study, we show that the inhibition of replication in Escherichia coli is transient even at concentrations of 0.1 M hydroxyurea a...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00713-17
更新日期:2018-02-23 00:00:00
abstract::Hurwitz, Charles (Veterans Administration Hospital, Albany, N.Y.) and Carmen L. Rosano. Chloramphenicol-sensitive and -insensitive phases of the lethal action of streptomycin. J. Bacteriol. 83:1202-1209. 1962.-If chloramphenicol is added to sensitive Escherichia coli cells at the same time as streptomycin, the lethal ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.83.6.1202-1209.1962
更新日期:1962-06-01 00:00:00
abstract::We previously described a gene, lpt3, required for the addition of phosphoethanolamine (PEtn) at the 3 position on the beta-chain heptose (HepII) of the inner-core Neisseria meningitidis lipopolysaccharide (LPS), but it has long been recognized that the inner-core LPS of some strains possesses PEtn at the 6 position (...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.186.20.6970-6982.2004
更新日期:2004-10-01 00:00:00
abstract::Isolated membrane vesicles from the obligately acidophilic bacterium Bacillus acidocaldarius generated an electrochemical gradient of protons (delta mu- H+) upon energization with ascorbate-phenazine methosulfate at pH 6.0 or 3.0. At pH 6.0, there was little or no transmembrane pH gradient (delta pH), but a transmembr...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.159.2.448-452.1984
更新日期:1984-08-01 00:00:00
abstract::The expression pattern of the Escherichia coli genome is controlled in part by regulating the utilization of a limited number of RNA polymerases among a total of its approximately 4,600 genes. The distribution pattern of RNA polymerase changes from modulation of two types of protein-protein interactions: the interacti...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01579-14
更新日期:2014-08-01 00:00:00