Streptokinase mutations relieving Escherichia coli K-12 (prlA4) of detriments caused by the wild-type skc gene.


:A novel phenotype is described for Escherichia coli K-12 carrying the prlA4 allele determining a membrane component of the protein export mechanism. It is manifest as transformation deficiency for plasmids containing the cloned group C streptococcal streptokinase gene, skc. Streptokinase plasmid mutations relieving the prlA4 strain of this deficiency fell into three classes. Class 1 included skc::IS5 insertions, with IS5 integrated in a region encoding the Skc signal sequence and inactivating skc. Class 2 included IS1 insertions leaving skc intact but reducing skc expression, presumably by altering the function of the skc promoter as judged by an insertion site close to the -35 region. The most interesting class, 3, included skc deletions removing the entire signal sequence or a tetrapeptide from its hydrophobic core. The tetrapeptide deletion reduced the size, hydrophobicity, and predicted alpha-helicity of the central region of the Skc signal sequence but facilitated the export of mature Skc in both the wild type and the prlA4 mutant. These findings indicate that the incompatibility between prlA4 and skc is related to deleterious effects of the Skc signal sequence. The tetrapeptide deletion may function by altering the conformation of the signal sequence so as to render interaction with both the PrlA wild-type protein and the PrlA4 mutant protein less detrimental to the export mechanism. These findings also provide an explanation for the difficulties encountered in cloning streptokinase genes in E. coli plasmids and maintaining their structural stability.


J Bacteriol


Journal of bacteriology


Müller J,Reinert H,Malke H




Has Abstract


1989-04-01 00:00:00












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    abstract::The genome sequence of the enteric bacterial pathogen Campylobacter jejuni NCTC 11168 (11168-GS) was published in 2000, providing a valuable resource for the identification of C. jejuni-specific colonization and virulence factors. Surprisingly, the 11168-GS clone was subsequently found to colonize 1-day-old chicks fol...

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    authors: Gaynor EC,Cawthraw S,Manning G,MacKichan JK,Falkow S,Newell DG

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  • Plasmid-determined enzymatic degradation of nylon oligomers.

    abstract::The nylon oligomer (6-aminohexanoic acid cyclic dimer) degradation genes on plasmid pOAD2 of Flavobacterium sp. KI72 were cloned into Escherichia coli vector pBR322. The locus of one of the genes, the structural gene of 6-aminohexanoic acid linear oligomer hydrolase, was determined by constructing various deletion pla...

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    authors: Negoro S,Taniguchi T,Kanaoka M,Kimura H,Okada H

    更新日期:1983-07-01 00:00:00

  • Identification of Zoogloea species and the relationship to zoogloeal matrix and floc formation.

    abstract::Three floc-forming, gram-negative, polarly flagellated rods were isolated and characterized. Our isolates were compared to four similar floc-forming organisms previously isolated in another laboratory and classified as two species of Zoogloea, one of Pseudomonas, and as one unidentified gram-negative rod. Possession o...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Friedman BA,Dugan PR

    更新日期:1968-05-01 00:00:00

  • Mutational analysis of the Shiga toxin and Shiga-like toxin II enzymatic subunits.

    abstract::The A-subunit polypeptides of Shiga toxin, the Shiga-like toxins (SLTs), and the plant lectin ricin inactivate eucaryotic ribosomes by enzymatically depurinating 28S rRNA. Comparison of the amino acid sequences of the members of the Shiga toxin family and ricin revealed two regions of significant homology that lie wit...

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    authors: Jackson MP,Deresiewicz RL,Calderwood SB

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  • Stability of beta-galactosidase messenger ribonucleic acid in Escherichia coli.

    abstract::Ben-Hamida, Fakher (Washington University School of Medicine, St. Louis, Mo.), and David Schlessinger. Stability of beta-galactosidase messenger ribonucleic acid in Escherichia coli. J. Bacteriol. 90:1611-1616. 1965.-Synthesis of beta-galactosidase stops within several minutes when preinduced, permeaseless cultures ar...

    journal_title:Journal of bacteriology

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    authors: Ben-Hamida F,Schlessinger D

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  • Exchange of genetic markers at extremely high temperatures in the archaeon Sulfolobus acidocaldarius.

    abstract::When cells of two auxotrophic mutants of Sulfolobus acidocaldarius are mixed and incubated on solid medium, they form stable genetic recombinants which can be selected, enumerated, and characterized. Any of a variety of auxotrophic markers can recombine with each other, and the phenomenon has been observed at temperat...

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  • A HilA-independent pathway to Salmonella typhimurium invasion gene transcription.

    abstract::Salmonella typhimurium invasion of nonphagocytic cells requires the expression of a type III secretion system (TTSS) encoded within Salmonella pathogenicity island 1 (SPI1). TTSS gene transcription is activated in response to environmental signals and requires transcriptional regulators encoded within (HilA) and outsi...

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    authors: Rakeman JL,Bonifield HR,Miller SI

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  • Structure of the low-affinity penicillin-binding protein 5 PBP5fm in wild-type and highly penicillin-resistant strains of Enterococcus faecium.

    abstract::Among its penicillin-binding proteins (PBPs), Enterococcus faecium possesses a low-affinity PBP5, PBP5fm, which is the main target involved in beta-lactam resistance. A 7.7-kb EcoRI chromosomal fragment of E. faecium D63r containing the pbp5fm gene was cloned and sequenced. Two open reading frames (ORFs) were found. A...

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    authors: Zorzi W,Zhou XY,Dardenne O,Lamotte J,Raze D,Pierre J,Gutmann L,Coyette J

    更新日期:1996-08-01 00:00:00

  • Kasugamycin-resistant mutants of Bacillus subtilis.

    abstract::Kasugamycin-resistant mutants of Bacillus subtilis were isolated and classified into two groups, one of which had resistance to kasugamycin in in vitro protein synthesis and mapped in the ribosomal region. The other group had no resistance to kasugamycin in in vitro protein synthesis and had weak cross-resistance to g...

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    pub_type: 杂志文章


    authors: Tominaga A,Kobayashi Y

    更新日期:1978-09-01 00:00:00

  • Genetic reconstitution of the high-affinity L-arabinose transport system.

    abstract::Expression plasmids containing various portions of araFGH operon sequences were assayed for their ability to facilitate the high-affinity L-arabinose transport process in a strain lacking the chromosomal copy of this operon. Accumulation studies demonstrated that the specific induction of all three operon coding seque...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Horazdovsky BF,Hogg RW

    更新日期:1989-06-01 00:00:00

  • Characterization of the signaling domain of the NO-responsive regulator NorR from Ralstonia eutropha H16 by site-directed mutagenesis.

    abstract::In Ralstonia eutropha H16, the nitric oxide (NO)-responsive transcriptional activator NorR controls the expression of a dicistronic operon that encodes a membrane-bound NO reductase, NorB, and a protein of unknown function, NorA. The N-terminal domain (NTD) of NorR is responsible for perception of the signal molecule,...

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    pub_type: 杂志文章


    authors: Klink A,Elsner B,Strube K,Cramm R

    更新日期:2007-04-01 00:00:00


    abstract::Domingue, Gerald J. (Tulane University, New Orleans, La.), and William A. Pierce, Jr. Effect of partially purified streptococcal M protein on the in vitro phagocytosis of Streptococcus pyogenes. J. Bacteriol. 89:583-588. 1965.-M protein from Streptococcus pyogenes strain Bailey type 12 was adsorbed onto cells of a glo...

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    更新日期:1965-03-01 00:00:00

  • The thiol:disulfide oxidoreductase DsbB mediates the oxidizing effects of the toxic metalloid tellurite (TeO32-) on the plasma membrane redox system of the facultative phototroph Rhodobacter capsulatus.

    abstract::The highly toxic oxyanion tellurite (TeO3(2-)) is a well known pro-oxidant in mammalian and bacterial cells. This work examines the effects of tellurite on the redox state of the electron transport chain of the facultative phototroph Rhodobacter capsulatus, in relation to the role of the thiol:disulfide oxidoreductase...

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    authors: Borsetti F,Francia F,Turner RJ,Zannoni D

    更新日期:2007-02-01 00:00:00

  • Evolutionary relationships among pathogenic Candida species and relatives.

    abstract::Small subunit rRNA sequences have been determined for 10 of the most clinically important pathogenic species of the yeast genus Candida (including Torulopsis [Candida] glabrata and Yarrowia [Candida] lipolytica) and for Hansenula polymorpha. Phylogenetic analyses of these sequences and those of Saccharomyces cerevisia...

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    authors: Barns SM,Lane DJ,Sogin ML,Bibeau C,Weisburg WG

    更新日期:1991-04-01 00:00:00

  • Phenotypic expression of mutations in a wide-host-range R plasmid in Escherichia coli and Rhizobium meliloti.

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  • Identification of genes encoding exported Mycobacterium tuberculosis proteins using a Tn552'phoA in vitro transposition system.

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  • Two trans-acting regulatory genes (vir and mod) control antigenic modulation in Bordetella pertussis.

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    authors: Knapp S,Mekalanos JJ

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  • Characterization of the Synechocystis strain PCC 6803 penicillin-binding proteins and cytokinetic proteins FtsQ and FtsW and their network of interactions with ZipN.

    abstract::Because very little is known about cell division in noncylindrical bacteria and cyanobacteria, we investigated 10 putative cytokinetic proteins in the unicellular spherical cyanobacterium Synechocystis strain PCC 6803. Concerning the eight penicillin-binding proteins (PBPs), which define three classes, we found that S...

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    authors: Marbouty M,Mazouni K,Saguez C,Cassier-Chauvat C,Chauvat F

    更新日期:2009-08-01 00:00:00

  • Opposite and Coordinated Rotation of Amphitrichous Flagella Governs Oriented Swimming and Reversals in a Magnetotactic Spirillum.

    abstract:UNLABELLED:Current knowledge regarding the mechanism that governs flagellar motor rotation in response to environmental stimuli stems mainly from the study of monotrichous and peritrichous bacteria. Little is known about how two polar flagella, one at each cell pole of the so-called amphitrichous bacterium, are coordin...

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  • NIC, a conjugative nicotine-nicotinate degradative plasmid in Pseudomonas convexa.

    abstract::The plasmid nature of genes specifying degradation of nicotine and nicotinate in Pseudomas convexa strain 1 (Pc1) is indicated by mitomycin curing and conjugational transfer to other strains. The NIC plasmid appears to be compatible with other metabolic plasmids in Pseudomonas putida. ...

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    authors: Thacker R,Rørvig O,Kahlon P,Gunsalus IC

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    journal_title:Journal of bacteriology

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    authors: Aslanidis C,Schmitt R

    更新日期:1990-04-01 00:00:00

  • Streptococcus salivarius fimbriae are composed of a glycoprotein containing a repeated motif assembled into a filamentous nondissociable structure.

    abstract::Streptococcus salivarius, a gram-positive bacterium found in the human oral cavity, expresses flexible peritrichous fimbriae. In this paper, we report purification and partial characterization of S. salivarius fimbriae. Fimbriae were extracted by shearing the cell surface of hyperfimbriated mutant A37 (a spontaneous m...

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    pub_type: 杂志文章


    authors: Lévesque C,Vadeboncoeur C,Chandad F,Frenette M

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  • Polymer-Producing Species of Arthrobacter.

    abstract::Gasdorf, Helen J. (Northern Regional Research Laboratory, Peoria, Ill.), R. G. Benedict, M. C. Cadmus, R. F. Anderson, and R. W. Jackson. Polymer-producing species of Arthrobacter. J. Bacteriol. 90:147-150.1965.-Two slime-producing microorganisms, designated as NRRL B-1973 and NRRL B-1797, were isolated from a Guatema...

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    pub_type: 杂志文章


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    pub_type: 杂志文章


    authors: Bleves S,Soscia C,Nogueira-Orlandi P,Lazdunski A,Filloux A

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    pub_type: 杂志文章


    authors: Oulmi L,Gorlas A,Gimenez G,Robert C,Boulahrouf A,Raoult D,Roux V

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    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: de Lorenzo V,Neilands JB

    更新日期:1986-07-01 00:00:00

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    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Canada KA,Iwashita S,Shim H,Wood TK

    更新日期:2002-01-01 00:00:00

  • Effects of cycloheximide and puromycin on synthesis of simian virus 40 T antigen in green monkey kidney cells.

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    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Gilden RV,Carp RI

    更新日期:1966-03-01 00:00:00

  • Estimation of gene expression in heterocysts of Anabaena variabilis by using DNA-RNA hybridization.

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    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Lynn ME,Bantle JA,Ownby JD

    更新日期:1986-09-01 00:00:00