Abstract:
:Uracil-DNA glycosylase activity was found in Streptococcus pneumoniae, and the enzyme was partially purified. An ung mutant lacking the activity was obtained by positive selection of cells transformed with a plasmid containing uracil in its DNA. The effects of the ung mutation on mutagenic processes in S. pneumoniae were examined. The sequence of several malM mutations revertible by nitrous acid showed them to correspond to A.T----G.C transitions. This confirmed a prior deduction that nitrous acid action on transforming DNA gave only G.C----A.T mutations. Examination of malM mutant reversion frequencies in ung strains indicated that G.C----A.T mutation rates generally were 10-fold higher than in wild-type strains, presumably owing to lack of repair of deaminated cytosine residues in DNA. No effect of ung on mutation avoidance by the Hex mismatch repair system was observed, which means that uracil incorporation and removal from nascent DNA cannot be solely responsible for producing strand breaks that target nascent DNA for correction after replication. One malM mutation corresponding to an A.T----G.C transition showed a 10-fold-higher spontaneous reversion frequency than other such transitions in a wild-type background. This "hot spot" was located in a directly repeated DNA sequence; it is proposed that transient slippage to the wild-type repeat during replication accounts for the higher reversion frequency.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Chen JD,Lacks SAdoi
10.1128/jb.173.1.283-290.1991subject
Has Abstractpub_date
1991-01-01 00:00:00pages
283-90issue
1eissn
0021-9193issn
1098-5530journal_volume
173pub_type
杂志文章abstract::Thin pili of the closely related IncI1 plasmids ColIb-P9 and R64 are required only for liquid mating and belong to the type IV family of pili. They were sedimented by ultracentrifugation from culture medium in which Escherichia coli cells harboring ColIb-P9- or R64-derived plasmids had been grown, and then the pili we...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.11.2842-2848.1998
更新日期:1998-06-01 00:00:00
abstract::The nucleotide sequence of tnaB of the tryptophanase operon of Escherichia coli is presented. TnaB is a tryptophan-specific permease that is homologous to Mtr, a second tryptophan-specific permease, and to TyrP, a tyrosine-specific permease. Each member of this family appears to contain 11 membrane-spanning domains. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.10.3231-3234.1991
更新日期:1991-05-01 00:00:00
abstract::The Rhizobium phaseoli recA gene has been cloned by interspecific complementation of the Fec phenotype of bacteriophage lambda. The cloned gene restored the recombination proficiency and conferred resistance to DNA-damaging agents (methyl methanesulfonate and nitrofurantoin) to an Escherichia coli recA mutant. The dir...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.10.3035-3040.1991
更新日期:1991-05-01 00:00:00
abstract::Between 59 and 60 min on the Escherichia coli genetic map, there is a highly conserved sequence of 29 base pairs, containing an inverted repeat of seven base pairs that appears 14 times, 32 or 33 base pairs apart, downstream of the iap gene coding region. About 24 kilobase pairs downstream of the 14 repeats, a similar...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.6.3553-3556.1989
更新日期:1989-06-01 00:00:00
abstract::Expression of the catalase-peroxidase of Caulobacter crescentus, a gram-negative member of the alpha subdivision of the Proteobacteria, is 50-fold higher in stationary-phase cultures than in exponential cultures. To identify regulators of the starvation response, Tn5 insertion mutants were isolated with reduced expres...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.19.6152-6159.1999
更新日期:1999-10-01 00:00:00
abstract::The porin composition of the Escherichia coli cell envelope was analyzed during growth at different external pHs (pHo) as a function of the acetyl phosphate (AcP) level (DeltaackA pta or ackA mutant, pyruvate or glucose as the carbon source) in the presence or absence of EnvZ. Our results indicate that the AcP level i...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.1.198-202.2000
更新日期:2000-01-01 00:00:00
abstract::Day, L. E. (Chas. Pfizer & Co., Inc., Groton, Conn.). Tetracycline inhibition of cell-free protein synthesis. II. Effect of the binding of tetracycline to the components of the system. J. Bacteriol. 92:197-203. 1966.-When tetracycline, an inhibitor of cell-free protein synthesis, was preincubated with each component o...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.92.1.197-203.1966
更新日期:1966-07-01 00:00:00
abstract::Staphylococcus aureus responds to changing extracellular environments in part by adjusting its proteome through alterations of transcriptional priorities and selective degradation of the preexisting pool of proteins. In Bacillus subtilis, the proteolytic adaptor protein MecA has been shown to play a role in assisting ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.02222-14
更新日期:2014-12-01 00:00:00
abstract::Comprehensive analysis of genome-wide expression patterns during growth of the hyperthermophilic bacterium Thermotoga maritima on 14 monosaccharide and polysaccharide substrates was undertaken with the goal of proposing carbohydrate specificities for transport systems and putative transcriptional regulators. Saccharid...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.21.7267-7282.2005
更新日期:2005-11-01 00:00:00
abstract::Bacterial carboxyl-terminal processing proteases (CTPs) are widely conserved and have been linked to important processes including signal transduction, cell wall metabolism, and virulence. However, the features that target proteins for CTP-dependent cleavage are unclear. Studies of the Escherichia coli CTP Prc suggest...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00174-20
更新日期:2020-06-01 00:00:00
abstract::Most bacteriophages (phages) release their progeny through the action of holins that form lesions in the cytoplasmic membrane and lysins that degrade the bacterial peptidoglycan. Although the function of each protein is well established in phages infecting Streptococcus pneumoniae, the role--if any--of the powerful ba...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00477-09
更新日期:2009-09-01 00:00:00
abstract::Bladen, Howard A. (National Institute of Dental Health, Bethesda, Md.), and Edward G. Hampp. Ultrastructure of Treponema microdentium and Borrelia vincentii. J. Bacteriol. 87:1180-1191.-A small oral Treponema (FM) and Borrelia vincentii (N9) were harvested after 3 to 7 days of incubation and either embedded in Vestopa...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.87.5.1180-1191.1964
更新日期:1964-05-01 00:00:00
abstract::A key step in the regulation of heat shock genes in Escherichia coli is the stress-dependent degradation of the heat shock promoter-specific sigma(32) subunit of RNA polymerase by the AAA protease, FtsH. Previous studies implicated the C termini of protein substrates, including sigma(32), as degradation signals for AA...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.20.5911-5917.2001
更新日期:2001-10-01 00:00:00
abstract::The O antigen is an important cell wall antigen of gram-negative bacteria, and the genes responsible for its biosynthesis are located in a gene cluster. We have cloned and sequenced the DNA segment unique to the O-antigen gene cluster of Salmonella enterica group D3. This segment includes a novel O-antigen polymerase ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.4.1002-1007.1998
更新日期:1998-02-01 00:00:00
abstract::Interest in finding small RNAs (sRNAs) in bacteria has significantly increased in recent years due to their regulatory functions. Development of high-throughput methods and more sophisticated computational algorithms has allowed rapid identification of sRNA candidates in different species. However, given their various...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.02359-14
更新日期:2015-01-01 00:00:00
abstract::Mutans of Pseudomonas testosteroni were isolated for their inability to grow on m-hydroxybenzoate as sole carbon source. These mutants hydroxylated m-hydroxybenzoate for form 2,3-dihydroxybenzoate in high yeilds. The bioconversion described in this report represents the first reported example of 3-hydroxybenzoate 2-hy...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.141.1.293-296.1980
更新日期:1980-01-01 00:00:00
abstract::Erythrobacter litoralis has been known as a bacteriochlorophyll a-containing, aerobic, anoxygenic, phototrophic bacterium. Here we announce the complete genome sequence of E. litoralis HTCC2594, which is devoid of phototrophic potential. E. litoralis HTCC2594, isolated by dilution-to-extinction culturing from seawater...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00026-09
更新日期:2009-04-01 00:00:00
abstract::The penicillin-binding proteins (PBPs) of 209 cell division (or growth) temperature-sensitive mutants of Streptococcus faecium were analyzed in this study. A total of nine strains showed either constitutive or temperature-sensitive conditional damage in the PBPs. Analysis of these nine strains yielded the following re...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.6.2432-2439.1987
更新日期:1987-06-01 00:00:00
abstract::This paper describes the major transmutations of donor deoxyribonucleic acid (DNA) after uptake by competent Bacillus subtilis cells. Kinetic experiments confirm that after exposure to competent cells, donor DNA is converted to double-stranded fragments (DSF) which can be isolated as early as 30 s from the beginning o...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.116.1.154-162.1973
更新日期:1973-10-01 00:00:00
abstract::Microtubular and lamellate membrane structures were observed at the log phase of growth in the stable L-forms of Escherichia coli cultured in the absence of penicillin. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.127.3.1564-1567.1976
更新日期:1976-09-01 00:00:00
abstract::The Ssa subfamily of Hsp70 molecular chaperones in the budding yeast Saccharomyces cerevisiae has four members, encoded by SSA1, SSA2, SSA3, and SSA4. Deletion of the two constitutively expressed genes, SSA1 and SSA2, results in cells which are slow growing and temperature sensitive. In this study, we demonstrate that...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.24.6484-6492.1998
更新日期:1998-12-01 00:00:00
abstract::Activation of the late prespore-specific RNA polymerase sigma factor sigma(G) during Bacillus subtilis sporulation coincides with completion of the engulfment process, when the prespore becomes a protoplast fully surrounded by the mother cell cytoplasm and separated from it by a double membrane system. Activation of s...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00715-08
更新日期:2008-12-01 00:00:00
abstract::Sinorhizobium meliloti is a soil-dwelling alphaproteobacterium that engages in a nitrogen-fixing root nodule symbiosis with leguminous plants. Cell surface polysaccharides are important both for adapting to stresses in the soil and for the development of an effective symbiotic interaction. Among the polysaccharides ch...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00501-17
更新日期:2018-01-10 00:00:00
abstract::Analysis of the Escherichia coli K10 capsule gene cluster identified two regions, regions 1 and 3, conserved between different group III capsule gene clusters. Region 1 encodes homologues of KpsD, KpsM, KpsT, and KpsE proteins, and region 3 encodes homologues of the KpsC and KpsS proteins. An rfaH mutation abolished K...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.7.2279-2285.1999
更新日期:1999-04-01 00:00:00
abstract::Escherichia coli LD0181 is sensitive to 15 micrograms of 2',3'-dideoxythymidine per ml. A derivative that was resistant to 40 micrograms of the same chemical per ml at 30 degrees C and that had lost the ability to grow on enriched medium at 42 degrees C was isolated after nitroso-guanidine mutagenesis. This mutant, TD...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.157.2.440-444.1984
更新日期:1984-02-01 00:00:00
abstract::In contrast to canonical phage endolysins, which require holin-mediated disruption of the membrane to gain access to attack the cell wall, signal anchor release (SAR) endolysins are secreted by the host sec system, where they accumulate in an inactive form tethered to the membrane by their N-terminal SAR domains. SAR ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00674-10
更新日期:2010-11-01 00:00:00
abstract::Biofilm formation was repressed by glucose in several species of Enterobacteriaceae. In Escherichia coli, this effect was mediated at least in part by cyclic AMP (cAMP)-cAMP receptor protein. A temporal role for cAMP in biofilm development was indicated by the finding that glucose addition after approximately 24 h fai...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.12.3406-3410.2002
更新日期:2002-06-01 00:00:00
abstract::To determine whether homologous recombination could be used to inactivate selected genes in Spiroplasma citri, plasmid constructs were designed to disrupt the motility gene scm1. An internal scm1 gene fragment was inserted into plasmid pKT1, which replicates in Escherichia coli but not in S. citri, and into the S. cit...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.24.7449-7456.1999
更新日期:1999-12-01 00:00:00
abstract::HetR plays a key role in regulation of heterocyst differentiation and patterning in Anabaena It directly regulates genes involved in heterocyst differentiation (such as hetP and hetZ), genes involved in pattern formation (patA), and many others. In this study, we investigated the functional relationship of hetP and he...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00707-17
更新日期:2018-04-09 00:00:00
abstract::Gram-negative Aggregatibacter actinomycetemcomitans can be distinguished (based on the promoter structure of the leukotoxin operon) into JP2 and non-JP2 genotypes, with the former found to be more pathogenic than the latter. Here we report the first complete genome sequence of a serotype b non-JP2 strain of A. actinom...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.06770-11
更新日期:2012-04-01 00:00:00