The C terminus of sigma(32) is not essential for degradation by FtsH.

Abstract:

:A key step in the regulation of heat shock genes in Escherichia coli is the stress-dependent degradation of the heat shock promoter-specific sigma(32) subunit of RNA polymerase by the AAA protease, FtsH. Previous studies implicated the C termini of protein substrates, including sigma(32), as degradation signals for AAA proteases. We investigated the role of the C terminus of sigma(32) in FtsH-dependent degradation by analysis of C-terminally truncated sigma(32) mutant proteins. Deletion of the 5, 11, 15, and 21 C-terminal residues of sigma(32) did not affect degradation in vivo or in vitro. Furthermore, a peptide comprising the C-terminal 21 residues of sigma(32) was not degraded by FtsH in vitro and thus did not serve as a recognition sequence for the protease, while an unrelated peptide of similar length was efficiently degraded. The truncated sigma(32) mutant proteins remained capable of associating with DnaK and DnaJ in vitro but showed intermediate (5-amino-acid deletion) and strong (11-, 15-, and 21-amino-acid deletions) defects in association with RNA polymerase in vitro and biological activity in vivo. These results indicate an important role for the C terminus of sigma(32) in RNA polymerase binding but no essential role for FtsH-dependent degradation and association of chaperones.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Tomoyasu T,Arsène F,Ogura T,Bukau B

doi

10.1128/JB.183.20.5911-5917.2001

keywords:

subject

Has Abstract

pub_date

2001-10-01 00:00:00

pages

5911-7

issue

20

eissn

0021-9193

issn

1098-5530

journal_volume

183

pub_type

杂志文章
  • Methanogenesis and ATP synthesis in a protoplast system of Methanobacterium thermoautotrophicum.

    abstract::When Methanobacterium thermoautotrophicum cells were incubated in 50 mM potassium phosphate buffer (pH 7.0) containing 1 M sucrose and autolysate from Methanobacterium wolfei, they were transformed into protoplasts. The protoplasts, which possessed no cell wall, lysed in buffer without sucrose. Unlike whole cells, the...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.168.2.892-900.1986

    authors: Mountfort DO,Mörschel E,Beimborn DB,Schönheit P

    更新日期:1986-11-01 00:00:00

  • DNA primase of plasmid ColIb is involved in conjugal DnA synthesis in donor and recipient bacteria.

    abstract::The sog gene of the IncI alpha group plasmid ColIb is known to encode a DNA primase that can substitute for defective host primase in dnaG mutants of Escherichia coli during discontinuous DNA replication. The biological significance of this enzyme was investigated by using sog mutants, constructed from a derivative of...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:

    authors: Chatfield LK,Orr E,Boulnois GJ,Wilkins BM

    更新日期:1982-12-01 00:00:00

  • Photooxidative death in blue-green algae.

    abstract::When incubated in the light under 100% oxygen, wild-type blue-green algae (Anacystis nidulans, Synechococcus cedrorum) die out rapidly at temperatures of 4 to 15 C, and at 35 C (or at 26 C in the case of S. cedrorum) in the absence of CO(2). Photosynthesis is impaired in these cells long before they die. Blocking of p...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.111.3.682-689.1972

    authors: Abeliovich A,Shilo M

    更新日期:1972-09-01 00:00:00

  • The YSIRK-G/S motif of staphylococcal protein A and its role in efficiency of signal peptide processing.

    abstract::Many surface proteins of pathogenic gram-positive bacteria are linked to the cell wall envelope by a mechanism requiring a C-terminal sorting signal with an LPXTG motif. Surface proteins of Streptococcus pneumoniae harbor another motif, YSIRK-G/S, which is positioned within signal peptides. The signal peptides of some...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.185.9.2910-2919.2003

    authors: Bae T,Schneewind O

    更新日期:2003-05-01 00:00:00

  • Cloning of the Pseudomonas aeruginosa outer membrane porin protein P gene: evidence for a linked region of DNA homology.

    abstract::The gene encoding the outer membrane phosphate-selective porin protein P from Pseudomonas aeruginosa was cloned into Escherichia coli. The protein product was expressed and transported to the outer membrane of an E. coli phoE mutant and assembled into functional trimers. Expression of a product of the correct molecula...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.170.5.2312-2318.1988

    authors: Siehnel RJ,Worobec EA,Hancock RE

    更新日期:1988-05-01 00:00:00

  • Functions in outer and inner membranes of Escherichia coli for ferrichrome transport.

    abstract::Mutants of the fhuA gene of Escherichia coli K-12, which encodes a receptor protein in the outer membrane, took up ferrichrome after exposure to pronase, whereas fhuB mutants remained transport negative. The latter finding supports our previous proposal that fhuB mutants are defective in a function that residues in th...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.146.3.1158-1161.1981

    authors: Wookey PJ,Hussein S,Braun V

    更新日期:1981-06-01 00:00:00

  • Inhibitory effect of diphtheria toxin on amino acid incorporation in Escherichia coli cell-free system.

    abstract::The mechanism of action of diphtheria toxin in an Escherichia coli cell-free protein-synthesizing system was examined. When the washed ribosomes were incubated with toxin before addition of messenger ribonucleic acid (RNA), peptide syntheses of (14)C-phenylalanine directed by polyuridylic acid or phage R17 RNA were st...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.104.1.152-157.1970

    authors: Tsugawa A,Ohsumi Y,Kato I

    更新日期:1970-10-01 00:00:00

  • Escherichia coli strains blocked in Tat-dependent protein export exhibit pleiotropic defects in the cell envelope.

    abstract::The Tat system is a recently discovered protein export pathway that serves to translocate folded proteins, often containing redox cofactors, across the bacterial cytoplasmic membrane. Here we report that tat strains are associated with a mutant cell septation phenotype, where chains of up to 10 cells are evident. Muta...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.1.139-144.2001

    authors: Stanley NR,Findlay K,Berks BC,Palmer T

    更新日期:2001-01-01 00:00:00

  • Peptidoglycan synthesis in cocci and rods of a pH-dependent, morphologically conditional mutant of Klebsiella pneumoniae.

    abstract::Mir M7 is a spontaneous morphologically conditional mutant of Klebsiella pneumoniae which grows as round cells (cocci) at pH 7 and as normal rods at pH 5.8. We studied the rates of peptidoglycan synthesis of cocci and rods growing at pH values of 7 and 5.8, respectively. It was found that exponentially growing cocci p...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.137.2.727-734.1979

    authors: Satta G,Fontana R,Canepari P,Botta G

    更新日期:1979-02-01 00:00:00

  • Roles of DctA and DctB in signal detection by the dicarboxylic acid transport system of Rhizobium leguminosarum.

    abstract::The dctA gene, coding for the dicarboxylate transport protein, has an inducible promoter dependent on activation by the two-component sensor-regulator pair DctB and DctD. LacZ fusion analysis indicates that there is a single promoter for dctB and dctD. The dctA promoter is also induced by nitrogen limitation, an effec...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.10.2660-2669.1998

    authors: Reid CJ,Poole PS

    更新日期:1998-05-01 00:00:00

  • Response and specificity of antibodies for Shigella flexneri: demonstration of type-specific factors in immunoglobulin G fraction of antiserum.

    abstract::Considerable amounts of immunoglobulin G (IgG) antibody appeared in hyperimmune rabbit serum at a late period during a course of immunization with several injections of Shigella flexneri O antigens. High yields of IgG antibody possessing homogenous specificity could be fractionated from crude gamma-globulin solution o...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.98.3.1128-1134.1969

    authors: Fukazawa Y,Shinoda T,Tsuchiya T

    更新日期:1969-06-01 00:00:00

  • Draft genome sequence of Virgibacillus halodenitrificans 1806.

    abstract::Virgibacillus halodenitrificans 1806 is an endospore-forming halophilic bacterium isolated from salterns in Korea. Here, we report the draft genome sequence of V. halodenitrificans 1806, which may reveal the molecular basis of osmoadaptation and insights into carbon and anaerobic metabolism in moderate halophiles. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01280-12

    authors: Lee SJ,Lee YJ,Jeong H,Lee SJ,Lee HS,Pan JG,Kim BC,Lee DW

    更新日期:2012-11-01 00:00:00

  • Evidence that TET protein functions as a multimer in the inner membrane of Escherichia coli.

    abstract::The inner membrane TET (TetA) protein, which is involved in Tn10-mediated microbial tetracycline resistance, consists of two domains, alpha and beta, both of which are needed for tetracycline resistance and efflux (M.S. Curiale, L.M. McMurry, and S.B. Levy, J. Bacteriol. 157:211-217, 1984). Since tetracycline-sensitiv...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.170.4.1715-1720.1988

    authors: Hickman RK,Levy SB

    更新日期:1988-04-01 00:00:00

  • Bacteria of the genus Bacillus have a hydrolase stereospecific to the D isomer of benzoyl-arginine-p-nitroanilide.

    abstract::A stereospecific enzyme activity capable of cleaving the amide bond of the synthetic substrate N-benzoyl-D-arginine-p-nitroanilide (D-BAPA) has been found in all aerobic and anaerobic members of the family Bacillaceae tested by us. Cells of nonsporeforming gram-positive or gram-negative bacteria contain a hydrolase ac...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.170.12.5895-5900.1988

    authors: Gofshtein-Gandman LV,Keynan A,Milner Y

    更新日期:1988-12-01 00:00:00

  • Growth pattern of Rickettsia tsutsugamushi in irradiated L cells.

    abstract::Irradiated L cells infected with Rickettsia tsutsugamushi were studied under the electron microscope to define the morphological growth pattern of the organism. For 2 days after inoculation, no rickettsiae were found either extra- or intracellularly; after 2 days multiple rickettsiae appeared within the host cells wit...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.154.2.879-892.1983

    authors: Hase T

    更新日期:1983-05-01 00:00:00

  • Osmotic remedial response in a galactose-negative mutant of Saccharomyces cerevisiae.

    abstract::We investigated an osmotic remedial mutant of Saccharomyces which was deficient in galactose-1-phosphate uridyl transferase. Both the rate of growth and the transferase activity of the mutant culture were dependent on the osmotic activity of the growth medium. Organic and ionic solutes proved to be equally effective i...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.95.3.1103-1110.1968

    authors: Bassel J,Douglas HC

    更新日期:1968-03-01 00:00:00

  • Functional and biochemical analysis of the Chlamydia trachomatis ligase MurE.

    abstract::Chlamydiae are unusual obligately intracellular bacteria that do not synthesize detectable peptidoglycan. However, they possess genes that appear to encode products with peptidoglycan biosynthetic activity. Bioinformatic analysis predicts that chlamydial MurE possesses UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelic acid (...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01029-09

    authors: Patin D,Bostock J,Blanot D,Mengin-Lecreulx D,Chopra I

    更新日期:2009-12-01 00:00:00

  • Regulation of bacterial cell walls: correlation between autolytic activity and cell wall turnover in Staphylococcus aureus.

    abstract::Cell wall turnover was examined in parent and mutant strains of Staphylococcus aureus. Peptidoglycan and teichoic acid were observed to undergo turnover in the wild-type strain during exponential growth; however, the rate of turnover did not decrease when the growth rate slowed, as the culture entered stationary phase...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.134.2.555-561.1978

    authors: Wong W,Chatterjee AN,Young FE

    更新日期:1978-05-01 00:00:00

  • Selection of araB and araC mutants of Escherichia coli B-r by resistance to ribitol.

    abstract::The growth of strain araC(c)67, which produces the enzymes of the ara operon constitutively, is inhibited by the addition of ribitol. Isolation of strains resistant to ribitol yields mutants of either the araB or araC genes. A model to account for the inhibition by ribitol is discussed. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.102.2.593-595.1970

    authors: Katz L

    更新日期:1970-05-01 00:00:00

  • Patterns of sequence conservation in the S-Layer proteins and related sequences in Clostridium difficile.

    abstract::Clostridium difficile is the etiological agent of antibiotic-associated diarrhea. Among the factors that may play a role in infection are S-layer proteins (SLPs). Previous work has shown these to consist mainly of two components, resulting from the cleavage of a precursor encoded by the slpA gene. The high-molecular-w...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.184.14.3886-3897.2002

    authors: Calabi E,Fairweather N

    更新日期:2002-07-01 00:00:00

  • Presence of polyphosphate of low molecular weight in zygomycetes.

    abstract::Polyphosphate of average chain length corresponding to 10 phosphate units was detected in the mycelial extract of zygomycetes. Gel electrophoresis techniques commonly used for the separation and characterization of acidic mucopolysaccharides were successfully used for the detection, purification, and characterization ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.127.3.1408-1413.1976

    authors: Dietrich SM

    更新日期:1976-09-01 00:00:00

  • Activation of spo0A transcription by sigma H is necessary for sporulation but not for competence in Bacillus subtilis.

    abstract::spo0A and spo0H are needed for the initiation of sporulation and for the development of genetic competence in Bacillus subtilis. Transcription of spo0A initiates from two promoters, Pv and Ps. Pv is active during vegetative growth and is recognized by RNA polymerase containing sigma A. Expression from Ps increases dur...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.12.3812-3815.1994

    authors: Siranosian KJ,Grossman AD

    更新日期:1994-06-01 00:00:00

  • Conservation of plasmid maintenance functions between linear and circular plasmids in Borrelia burgdorferi.

    abstract::The Lyme disease agent Borrelia burgdorferi maintains both linear and circular plasmids that appear to be essential for mammalian infection. Recent studies have characterized the circular plasmid regions that confer autonomous replication, but the genetic elements necessary for linear plasmid maintenance have not been...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.185.10.3202-3209.2003

    authors: Stewart PE,Chaconas G,Rosa P

    更新日期:2003-05-01 00:00:00

  • INFLUENCE OF ROUTE OF INFECTION AND OTHER FACTORS ON GROWTH AND DISTRIBUTION OF LISTERIA MONOCYTOGENES IN ORGANS OF MICE.

    abstract::Silverman, Sidney J. (U.S. Army Biological Laboratories, Fort Detrick, Frederick, Md.), Lynn P. Elwell, and James F. Drawdy. Influence of route of infection and other factors on growth and distribution of Listeria monocytogenes in organs of mice. J. Bacteriol. 86:355-362. 1963.-The growth of Listeria monocytogenes in ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.86.3.355-362.1963

    authors: SILVERMAN SJ,ELWELL LP,DRAWDY JF

    更新日期:1963-09-01 00:00:00

  • Transformability of streptomycin-resistant group H streptococci.

    abstract::Several resistant mutants of a transformable group H streptococcus, strain Challis, were isolated from media containing high concentrations of streptomycin. Mutants SR5a and SR5 exhibited high and low transformability, respectively, when exposed to deoxyribonucleic acid (DNA) from a novobiocin-resistant Challis strain...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.95.1.132-138.1968

    authors: Perry D

    更新日期:1968-01-01 00:00:00

  • Phage-associated mutator phenotype in group A streptococcus.

    abstract::Defects in DNA mismatch repair (MMR) occur frequently in natural populations of pathogenic and commensal bacteria, resulting in a mutator phenotype. We identified a unique genetic element in Streptococcus pyogenes strain SF370 that controls MMR via a dynamic process of prophage excision and reintegration in response t...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01569-07

    authors: Scott J,Thompson-Mayberry P,Lahmamsi S,King CJ,McShan WM

    更新日期:2008-10-01 00:00:00

  • Cyanobacterial transposons Tn5469 and Tn5541 represent a novel noncomposite transposon family.

    abstract::A noncomposite transposon, designated Tn5541, was isolated from strain Fd33 of the filamentous cyanobacterium Fremyella diplosiphon UTEX 481. Sequence analysis showed that Tn5541 is structurally and genetically very similar to Tn5469, which is also endogenous to F. diplosiphon. Both Tn5469 and Tn5541 encode homologous...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.22.6059-6063.1998

    authors: Schaefer MR,Kahn K

    更新日期:1998-11-01 00:00:00

  • Control of diaminopimelate decarboxylase by L-lysine during growth and sporulation of Bacilluscereus.

    abstract::l-Lysine caused repression of diaminopimelate decarboxylase synthesis in Bacillus cereus when grown in either a minimal defined medium (CDGS medium) or a complex defined medium (a modified lysine assay medium). When cells were grown in either of the two media, variations in the specific activity of the enzyme as a fun...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.106.2.551-560.1971

    authors: Grandgenett DP,Stahly DP

    更新日期:1971-05-01 00:00:00

  • Regulation and timing of deoxyribonucleic acid synthesis in hyphae of Aspergillus nidulans.

    abstract::Pulse labeling of deoxyribonucleic acid (DNA) and radioautography have been used to study the effect of growth rate on nuclear replication in Aspergillus nidulans. When conidia were germinated in media supporting a fast growth rate, the radioactive pulse labeled either all of the nuclei in a cell or none of them. At s...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.95.6.2275-2281.1968

    authors: Kessel M,Rosenberger RF

    更新日期:1968-06-01 00:00:00

  • Participation of an extracellular deaminase in amino acid utilization by Neurospora crassa.

    abstract::A strain of Neurospora crassa defective in amino acid transport can utilize a variety of amino acids for growth when readily metabolizable nitrogen is limiting. Growth is accompanied by the production of an extracellular deaminase that converts the amino acid to its respective keto acid plus equimolar quantities of ut...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.159.2.583-589.1984

    authors: DeBusk RM,Ogilvie S

    更新日期:1984-08-01 00:00:00