Processing of a membrane protein required for cell-to-cell signaling during endospore formation in Bacillus subtilis.

Abstract:

:Activation of the late prespore-specific RNA polymerase sigma factor sigma(G) during Bacillus subtilis sporulation coincides with completion of the engulfment process, when the prespore becomes a protoplast fully surrounded by the mother cell cytoplasm and separated from it by a double membrane system. Activation of sigma(G) also requires expression of spoIIIJ, coding for a membrane protein translocase of the YidC/Oxa1p/Alb3 family, and of the mother cell-specific spoIIIA operon. Here we present genetic and biochemical evidence indicating that SpoIIIAE, the product of one of the spoIIIA cistrons, and SpoIIIJ interact in the membrane, thereby linking the function of the spoIIIJ and spoIIIA loci in the activation of sigma(G). We also show that SpoIIIAE has a functional Sec-type signal peptide, which is cleaved during sporulation. Furthermore, mutations that reduce or eliminate processing of the SpoIIIAE signal peptide arrest sporulation following engulfment completion and prevent activation of sigma(G). SpoIIIJ-type proteins can function in cooperation with or independently of the Sec system. In one model, SpoIIIJ interacts with SpoIIIAE in the context of the Sec translocon to promote its correct localization and/or topology in the membrane, so that it can signal the activation of sigma(G) following engulfment completion.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Serrano M,Vieira F,Moran CP Jr,Henriques AO

doi

10.1128/JB.00715-08

subject

Has Abstract

pub_date

2008-12-01 00:00:00

pages

7786-96

issue

23

eissn

0021-9193

issn

1098-5530

pii

JB.00715-08

journal_volume

190

pub_type

杂志文章
  • Motility and chemotaxis of Spirochaeta aurantia: computer-assisted motion analysis.

    abstract::A computer program has been designed to study behavior in populations of Spirochaeta aurantia cells, and this program has been used to analyze changes in behavior in response to chemoattractants. Three kinds of behavior were distinguished: smooth swimming, flexing, and reversals in direction of swimming after a short ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.170.4.1768-1774.1988

    authors: Fosnaugh K,Greenberg EP

    更新日期:1988-04-01 00:00:00

  • Conditional lethality of deletions which include uvrB in strains of Escherichia coli lacking deoxyribonucleic acid polymerase I.

    abstract::Deletions of the uvrB gene were not obtained in polA1 strains of Escherichia coli either by selecting for spontaneous deletions or by transduction from strains carrying such deletions. A strain forming a temperature-sensitive deoxyribonucleic acid polymerase I and carrying a deletion of the uvrB gene is inviable at th...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.112.2.676-681.1972

    authors: Shizuya H,Dykhuizen D

    更新日期:1972-11-01 00:00:00

  • Bacterial-type ferredoxin genes in the nitrogen fixation regions of the cyanobacterium Anabaena sp. strain PCC 7120 and Rhizobium meliloti.

    abstract::The nucleotide sequence of a region located downstream of the nifB gene, both in the cyanobacterium Anabaena sp. strain PCC 7120 and in Rhizobium meliloti, has been determined. This region contains a gene (fdxN) whose predicted polypeptide product strongly resembles typical bacterial ferredoxins. Cyanobacteria have no...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.170.9.4406-4410.1988

    authors: Mulligan ME,Buikema WJ,Haselkorn R

    更新日期:1988-09-01 00:00:00

  • Draft genome sequence of Mycobacterium bolletii strain M24, a rapidly growing mycobacterium of contentious taxonomic status.

    abstract::The whole-genome sequence of Mycobacterium bolletii M24, isolated from the bronchoalveolar lavage fluid of a Malaysian patient, is reported here. The circular chromosome of 5,507,730 bp helped to clarify the taxonomic position of this organism within the M. abscessus complex and revealed the presence of proteins poten...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00916-12

    authors: Wong YL,Choo SW,Tan JL,Ong CS,Ng KP,Ngeow YF

    更新日期:2012-08-01 00:00:00

  • SAL-TOL in vivo recombinant plasmid pKF439.

    abstract::SAL-TOL in vivo recombinant plasmid pKF439 was characterized in a strain from a mixed culture of bacteria harboring various degradative plasmids. Analysis of the gene organization of pKF439 revealed that the 57-kilobase TOL fragment, including the 40-kilobase TOL metabolic region, was inserted into the complete SAL re...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.162.3.1325-1328.1985

    authors: Furukawa K,Miyazaki T,Tomizuka N

    更新日期:1985-06-01 00:00:00

  • Metabolism of dibutylphthalate and phthalate by Micrococcus sp. strain 12B.

    abstract::Micrococcus sp. strain 12B was isolated by enriching for growth with dibutylphthalate as the sole carbon and energy source. A pathway for the metabolism of dibutylphthalate and phthalate by micrococcus sp. strain 12B is proposed: dibutylphthalate leads to monobutylphthalate leads to phthalate leads to 3,4-dihydro-3,4-...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.151.1.48-57.1982

    authors: Eaton RW,Ribbons DW

    更新日期:1982-07-01 00:00:00

  • Two roles for the DNA recognition site of the Klebsiella aerogenes nitrogen assimilation control protein.

    abstract::The nitrogen assimilation control protein (NAC) binds to a site within the promoter region of the histidine utilization operon (hutUH) of Klebsiella aerogenes, and NAC bound at this site activates transcription of hutUH. This NAC-binding site was characterized by a combination of random and directed DNA mutagenesis. M...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.3.578-585.1998

    authors: Pomposiello PJ,Janes BK,Bender RA

    更新日期:1998-02-01 00:00:00

  • Myxospore induction in a nondispersed growing mutant of Myxococcus xanthus.

    abstract::Myxococcus xanthus RB5, a rough-colony-forming, nondispersed growing mutant of strain FBt, forms macroscopic, multicellular masses of radially oriented cells in shake cultures. The cells appear to be held together by slime fibrils. Physical and enzymatic methods to disrupt the spheres were unsuccessful as were attempt...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.122.1.302-306.1975

    authors: Burchard RP

    更新日期:1975-04-01 00:00:00

  • ArcB1, a homolog of Escherichia coli ArcB, regulates dimethyl sulfoxide reduction in Shewanella oneidensis MR-1.

    abstract::Shewanella oneidensis is a metal reducer that uses the cyclic AMP receptor protein, CRP, to regulate anaerobic respiration. In addition, ArcA(So) is required for anaerobic growth with dimethyl sulfoxide (DMSO) and plays a role in aerobic respiration. The sensor kinase that activates ArcA(So) in S. oneidensis is not kn...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01695-09

    authors: Shroff NP,Charania MA,Saffarini DA

    更新日期:2010-06-01 00:00:00

  • Cloning and sequencing of a 2,5-dichlorohydroquinone reductive dehalogenase gene whose product is involved in degradation of gamma-hexachlorocyclohexane by Sphingomonas paucimobilis.

    abstract::Sphingomonas (formerly Pseudomonas) paucimobilis UT26 utilizes gamma-hexachlorocyclohexane (gamma-HCH), a halogenated organic insecticide, as a sole carbon and energy source. In a previous study, we showed that gamma-HCH is degraded to 2,5-dichlorohydroquinone (2,5-DCHQ) (Y. Nagata, R. Ohtomo, K. Miyauchi, M. Fukuda, ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.6.1354-1359.1998

    authors: Miyauchi K,Suh SK,Nagata Y,Takagi M

    更新日期:1998-03-01 00:00:00

  • The eutD gene of Salmonella enterica encodes a protein with phosphotransacetylase enzyme activity.

    abstract::The EutD protein of Salmonella enterica is homologous to the catalytic domain of the phosphotransacetylase (Pta) enzyme. The Pta-like activity level of the EutD enzyme compared favorably to that of other Pta enzymes. High-pressure liquid chromatography and mass spectrometry verified that acetyl-coenzyme A was the prod...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.186.6.1890-1892.2004

    authors: Brinsmade SR,Escalante-Semerena JC

    更新日期:2004-03-01 00:00:00

  • Preferential selection of deletion mutations of the outer membrane lipoprotein gene of Escherichia coli by globomycin.

    abstract::Globomycin is an antibiotic which inhibits the processing of the prolipoprotein. Eighty globomycin-resistant mutants were independently isolated from Escherichia coli K-12 which had a deletion mutation in chromosomal lipoprotein gene (lpp), but contained a plasmid carrying the wild-type lpp gene. Twenty-six of the mut...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.145.1.654-656.1981

    authors: Zwiebel LJ,Inukai M,Nakamura K,Inouye M

    更新日期:1981-01-01 00:00:00

  • The Escherichia coli Lpt transenvelope protein complex for lipopolysaccharide export is assembled via conserved structurally homologous domains.

    abstract::Lipopolysaccharide is a major glycolipid component in the outer leaflet of the outer membrane (OM), a peculiar permeability barrier of Gram-negative bacteria that prevents many toxic compounds from entering the cell. Lipopolysaccharide transport (Lpt) across the periplasmic space and its assembly at the Escherichia co...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.02057-12

    authors: Villa R,Martorana AM,Okuda S,Gourlay LJ,Nardini M,Sperandeo P,Dehò G,Bolognesi M,Kahne D,Polissi A

    更新日期:2013-03-01 00:00:00

  • Construction of a tyrP-lac operon fusion strain and its use in the isolation and analysis of mutants derepressed for tyrP expression.

    abstract::The gene tyrP, which codes for a component of the tyrosine-specific transport system, has been localized on the Escherichia coli K-12 chromosome at min 42. A tyrP-lac operon fusion was constructed and used to isolate mutants that have altered expression from the tyrP promoter. All putative tyrP operator mutations were...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.160.1.175-183.1984

    authors: Kasian PA,Pittard J

    更新日期:1984-10-01 00:00:00

  • Escherichia coli fimbriae recognizing sialyl galactosides.

    abstract::Fimbriae recognizing sialyl galactosides (S fimbriae) were purified from an Escherichia coli strain. The S fimbriae were morphologically identical to type 1 and P fimbriae of E. coli and showed a hemagglutination that was abolished when erythrocytes were treated with neuraminidase. Hemagglutination by the purified fim...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.159.2.762-766.1984

    authors: Korhonen TK,Väisänen-Rhen V,Rhen M,Pere A,Parkkinen J,Finne J

    更新日期:1984-08-01 00:00:00

  • Mutagenesis of specificity and toxicity regions of a Bacillus thuringiensis protoxin gene.

    abstract::Two different 30-nucleotide regions of the cryIAc insecticidal protoxin gene from Bacillus thuringiensis were randomly mutagenized. One region was within one of seven amphipathic helices believed to be important for the formation of ion channels. There was no loss of toxicity for three test insects by any of 27 mutant...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.14.4059-4065.1995

    authors: Aronson AI,Wu D,Zhang C

    更新日期:1995-07-01 00:00:00

  • Characteristics and osmoregulatory roles of uptake systems for proline and glycine betaine in Lactococcus lactis.

    abstract::Lactococcus lactis subsp. lactis ML3 contains high pools of proline or betaine when grown under conditions of high osmotic strength. These pools are created by specific transport systems. A high-affinity uptake system for glycine betaine (betaine) with a Km of 1.5 microM is expressed constitutively. The activity of th...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.17.5438-5444.1993

    authors: Molenaar D,Hagting A,Alkema H,Driessen AJ,Konings WN

    更新日期:1993-09-01 00:00:00

  • Genomic insights into the convergence and pathogenicity factors of Campylobacter jejuni and Campylobacter coli species.

    abstract::Whether or not bacteria form coherent evolutionary groups via means of genetic exchange and, hence, elicit distinct species boundaries remains an unsettled issue. A recent report implied that not only may the former be true but also, in fact, the clearly distinct Campylobacter jejuni and Campylobacter coli species may...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00519-09

    authors: Caro-Quintero A,Rodriguez-Castaño GP,Konstantinidis KT

    更新日期:2009-09-01 00:00:00

  • Glycerol metabolism and PrfA activity in Listeria monocytogenes.

    abstract::Listeria monocytogenes is able to efficiently utilize glycerol as a carbon source. In a defined minimal medium, the growth rate (during balanced growth) in the presence of glycerol is similar to that in the presence of glucose or cellobiose. Comparative transcriptome analyses of L. monocytogenes showed high-level tran...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00259-08

    authors: Joseph B,Mertins S,Stoll R,Schär J,Umesha KR,Luo Q,Müller-Altrock S,Goebel W

    更新日期:2008-08-01 00:00:00

  • Structural characterization and assembly of the distal tail structure of the temperate lactococcal bacteriophage TP901-1.

    abstract::The tail structures of bacteriophages infecting gram-positive bacteria are largely unexplored, although the phage tail mediates the initial interaction with the host cell. The temperate Lactococcus lactis phage TP901-1 of the Siphoviridae family has a long noncontractile tail with a distal baseplate. In the present st...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.187.12.4187-4197.2005

    authors: Vegge CS,Brøndsted L,Neve H,Mc Grath S,van Sinderen D,Vogensen FK

    更新日期:2005-06-01 00:00:00

  • Membrane association of conjugally transferred deoxyribonucleic acid in Escherichia coli minicells.

    abstract::The conjugally acquired deoxyribonucleic acid (DNA) of small, anucleate cells ("minicells") of a mutant strain of Escherichia coli K-12 was found to be predominantly associated with the bacterial membrane. Evidence from X-irradiation studies in vivo shows that there is no decrease in DNA-membrane association under con...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.106.2.626-633.1971

    authors: Shull FW Jr,Fralick JA,Stratton LP,Fisher WD

    更新日期:1971-05-01 00:00:00

  • Properties of adenyl cyclase and cyclic adenosine 3',5'-monophosphate receptor protein-deficient mutants of Escherichia coli.

    abstract::Several spontaneous cya and crp mutants of Escherichia coli have been selected as clones simultaneously resistant to phage lambda and nalidixic acid and characterized. Both cya and crp mutants have been found to grow as cocci with increased doubling times. They have increased resistance to some mutagens (methylmethane...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.125.2.545-555.1976

    authors: Kumar S

    更新日期:1976-02-01 00:00:00

  • Staphylococcal bacteriophage-associated lysin: a lytic agent active against Staphylococcus aureus.

    abstract::A lytic enzyme active against viable, intact staphylococci is released into culture fluids upon lysis of bacteriophage-infected Staphylococcus aureus PS53 cells. This enzyme, staphylococcal phage-associated lysin (PAL), was partially purified by ammonium sulfate precipitation and gel filtration through Sephadex G-200....

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.107.2.499-504.1971

    authors: Sonstein SA,Hammel JM,Bondi A

    更新日期:1971-08-01 00:00:00

  • GENETIC INSTABILITY IN ESCHERICHIA COLI.

    abstract::Schwartz, Norman M. (Yale University, New Haven, Conn.). Genetic instability in Escherichia coli. J. Bacteriol. 89:712-717. 1965.-Results obtained from a transductional analysis demonstrated that an extremely unstable reversion of lac(-) is a suppressor mutation. This mutation suppresses 2 of 22 different lac(-) allel...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.89.3.712-717.1965

    authors: SCHWARTZ NM

    更新日期:1965-03-01 00:00:00

  • Glucose catabolism in Micrococcus sodonensis.

    abstract::The inability of Micrococcus sodonensis to grow on glucose as the sole source of carbon and energy was investigated. Estimation of pathways of glucose catabolism indicated that both the glycolytic and hexose monophosphate pathways are present in this organism. Comparative studies with Escherichia coli demonstrated tha...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.93.6.1839-1846.1967

    authors: Perry JJ,Evans JB

    更新日期:1967-06-01 00:00:00

  • Functional characterization of a novel xylanase from a corn strain of Erwinia chrysanthemi.

    abstract::A beta-1,4-xylan hydrolase (xylanase A) produced by Erwinia chrysanthemi D1 isolated from corn was analyzed with respect to its secondary structure and enzymatic function. The pH and temperature optima for the enzyme were found to be pH 6.0 and 35 degrees C, with a secondary structure under those conditions that consi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.6.2093-2100.2001

    authors: Hurlbert JC,Preston JF 3rd

    更新日期:2001-03-01 00:00:00

  • Expression of a Saccharomyces cerevisiae photolyase gene in Escherichia coli.

    abstract::A 3.3-kilobase PvuII fragment carrying the PHR1 gene of Saccharomyces cerevisiae has been cloned into an Escherichia coli expression vector and introduced into E. coli strains deficient in DNA photolyase. Complementation of the E. coli phr-1 mutation was observed, strongly suggesting that the yeast PHR1 gene encodes a...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.161.2.769-771.1985

    authors: Sancar GB

    更新日期:1985-02-01 00:00:00

  • The Tn10-encoded tetracycline resistance mRNA contains a translational silencer in the 5' nontranslated region.

    abstract::We performed a mutational analysis of the left half of Tn10-encoded tet operator O2, located in the 5' nontranslated region of the mRNA for the resistance protein TetA, and determined the importance of that region for translation efficiency and mRNA stability. Transcriptional fusions of 17 mutants to lacZ expressed th...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.8.2478-2484.1992

    authors: Flache P,Baumeister R,Hillen W

    更新日期:1992-04-01 00:00:00

  • Gene inactivation in Lactococcus lactis: branched-chain amino acid biosynthesis.

    abstract::The Lactococcus lactis subsp. lactis strains isolated from dairy products are auxotrophs for branched-chain amino acids (leucine, isoleucine, and valine), while most strains isolated from nondairy media are prototrophs. We have cloned and sequenced the leu genes from one auxotroph, IL1403. The sequence is 99% homologo...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.14.4383-4390.1993

    authors: Godon JJ,Delorme C,Bardowski J,Chopin MC,Ehrlich SD,Renault P

    更新日期:1993-07-01 00:00:00

  • METHICILLIN-INDUCED LYSOZYME-SENSITIVE FORMS OF STAPHYLOCOCCI.

    abstract::Aldrich, K. M. (The University of Kansas, Lawrence), and C. P. Sword. Methicillin-induced lysozyme-sensitive forms of staphylococci. J. Bacteriol. 87:690-695. 1964.-Staphylococcus aureus and S. epidermidis grown in the presence of sublethal amounts of methicillin were converted to enlarged spheres within 2 to 4 hr, as...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.87.3.690-695.1964

    authors: ALDRICH KM,SWORD CP

    更新日期:1964-03-01 00:00:00