Structural characterization and assembly of the distal tail structure of the temperate lactococcal bacteriophage TP901-1.

Abstract:

:The tail structures of bacteriophages infecting gram-positive bacteria are largely unexplored, although the phage tail mediates the initial interaction with the host cell. The temperate Lactococcus lactis phage TP901-1 of the Siphoviridae family has a long noncontractile tail with a distal baseplate. In the present study, we investigated the distal tail structures and tail assembly of phage TP901-1 by introducing nonsense mutations into the late transcribed genes dit (orf46), tal(TP901-1) (orf47), bppU (orf48), bppL (orf49), and orf50. Transmission electron microscopy examination of mutant and wild-type TP901-1 phages showed that the baseplate consisted of two different disks and that a central tail fiber is protruding below the baseplate. Evaluation of the mutant tail morphologies with protein profiles and Western blots revealed that the upper and lower baseplate disks consist of the proteins BppU and BppL, respectively. Likewise, Dit and Tal(TP901-1) were shown to be structural tail proteins essential for tail formation, and Tal(TP901-1) was furthermore identified as the tail fiber protein by immunogold labeling experiments. Determination of infection efficiencies of the mutant phages showed that the baseplate is fundamental for host infection and the lower disk protein, BppL, is suggested to interact with the host receptor. In contrast, ORF50 was found to be nonessential for tail assembly and host infection. A model for TP901-1 tail assembly, in which the function of eight specific proteins is considered, is presented.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Vegge CS,Brøndsted L,Neve H,Mc Grath S,van Sinderen D,Vogensen FK

doi

10.1128/JB.187.12.4187-4197.2005

keywords:

subject

Has Abstract

pub_date

2005-06-01 00:00:00

pages

4187-97

issue

12

eissn

0021-9193

issn

1098-5530

pii

187/12/4187

journal_volume

187

pub_type

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