Abstract:
:Lactococcus lactis subsp. lactis ML3 contains high pools of proline or betaine when grown under conditions of high osmotic strength. These pools are created by specific transport systems. A high-affinity uptake system for glycine betaine (betaine) with a Km of 1.5 microM is expressed constitutively. The activity of this system is not stimulated by high osmolarities of the growth or assay medium but varies strongly with the medium pH. A low-affinity proline uptake system (Km, > 5 mM) is expressed at high levels only in chemically defined medium (CDM) with high osmolarity. This transport system is also stimulated by high osmolarity. The expression of this proline uptake system is repressed in rich broth with low or high osmolarity and in CDM with low osmolarity. The accumulated proline can be exchanged for betaine. Proline uptake is also effectively inhibited by betaine (Ki of between 50 and 100 microM). The proline transport system therefore probably also transports betaine. The inhibition of proline transport by betaine results in low proline pools in cells grown in high-osmotic-strength, betaine-containing CDM. The energy and pH dependency and the influence of ionophores on the activity of both transport systems suggest that these systems are not proton motive force driven. At low osmolarities, proline uptake is low but significant. This low proline uptake is also inhibited by betaine, although to a lesser extent than in cells grown in high-osmotic-strength CDM. These data indicate that proline uptake in L. lactis is enzyme mediated and is not dependent on passive diffusion, as was previously believed.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Molenaar D,Hagting A,Alkema H,Driessen AJ,Konings WNdoi
10.1128/jb.175.17.5438-5444.1993subject
Has Abstractpub_date
1993-09-01 00:00:00pages
5438-44issue
17eissn
0021-9193issn
1098-5530journal_volume
175pub_type
杂志文章abstract::We have identified three new Haemophilus influenzae mutations causing cells to exhibit extreme hypercompetence at all stages of growth. The mutations are in murE, which encodes the meso-diaminopimelate-adding enzyme of peptidoglycan synthesis. All are point mutations causing nonconservative amino acid substitutions, t...
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更新日期:1984-05-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1964-06-01 00:00:00
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pub_type: 杂志文章
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更新日期:1998-11-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JB.145.1.145-155.1981
更新日期:1981-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:1970-04-01 00:00:00
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更新日期:1963-02-01 00:00:00
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更新日期:1989-02-01 00:00:00
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更新日期:1976-04-01 00:00:00
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更新日期:2006-09-01 00:00:00
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更新日期:2000-02-01 00:00:00
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更新日期:2000-05-01 00:00:00
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pub_type: 杂志文章
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更新日期:1979-09-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.122.1.59-65.1975
更新日期:1975-04-01 00:00:00
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pub_type: 杂志文章
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更新日期:1999-02-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1978-06-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.122.3.810-817.1975
更新日期:1975-06-01 00:00:00