The Escherichia coli Lpt transenvelope protein complex for lipopolysaccharide export is assembled via conserved structurally homologous domains.

abstract：:The levels of reduced and oxidized nicotinamide adenine dinucleotides were determined in Xanthobacter flavus during a transition from heterotrophic to autotrophic growth. Excess reducing equivalents are rapidly dissipated following induction of the Calvin cycle, indicating that the Calvin cycle serves as a sink for ex...

journal_title：Journal of bacteriology

authors： van Keulen G,Dijkhuizen L,Meijer WG

更新日期：2000-08-01 00:00:00

abstract：:NADPH is an intermediate in the oxidation of organic compounds coupled to Fe(III) reduction in Geobacter species, but Fe(III) reduction with NADPH as the electron donor has not been studied in these organisms. Crude extracts of Geobacter sulfurreducens catalyzed the NADPH-dependent reduction of Fe(III)-nitrilotriaceti...

journal_title：Journal of bacteriology

authors： Kaufmann F,Lovley DR

更新日期：2001-08-01 00:00:00

abstract：:The inducible temperate bacteriophage phi75 and a clear-plaque-forming variant, phi75C1, mediated transduction of a 4.4 X 10(6)-dalton multicopy Bacillus pumilus plasmid, pPL10, at frequencies of 10(-5) to 10(-6) transductants per plaque-forming unit. phi75- and phi75C1-mediated transduction of several chromosome mark...

journal_title：Journal of bacteriology

authors： Bramucci MG,Lovett PS

更新日期：1977-09-01 00:00:00

abstract：:Mycobacterium tuberculosis utilizes the methylerythritol phosphate (MEP) pathway for biosynthesis of isopentenyl diphosphate and its isomer, dimethylallyl diphosphate, precursors of all isoprenoid compounds. This pathway is of interest as a source of new drug targets, as it is absent from humans and disruption of the ...

journal_title：Journal of bacteriology

authors： Eoh H,Brown AC,Buetow L,Hunter WN,Parish T,Kaur D,Brennan PJ,Crick DC

更新日期：2007-12-01 00:00:00

abstract：:Mycobacterium tuberculosis has a clonal population structure, and the Latin American-Mediterranean (LAM) family is one of the largest and most widespread within this species, showing evidence for remarkable pathobiology and a confusing phylogeny. Here, we applied robust phylogenetic markers to study the evolution of t...

journal_title：Journal of bacteriology

authors： Mokrousov I,Vyazovaya A,Narvskaya O

更新日期：2014-05-01 00:00:00

abstract：:The host range of an octopine Ti plasmid is limited to Rhizobiaceae. This has been extended also to Escherichia coli in the form of a stable cointegrate with the wide-host-range plasmid R772. Its structure was studied by constructing a physical map of R772 and of the R772::pTiB6 cointegrate. An insertion sequence pres...

journal_title：Journal of bacteriology

authors： Hille J,van Kan J,Klasen I,Schilperoort R

更新日期：1983-05-01 00:00:00

abstract：:During growth of the halophilic archaeon Haloarcula marismortui on D-xylose, a specific D-xylose dehydrogenase was induced. The enzyme was purified to homogeneity. It constitutes a homotetramer of about 175 kDa and catalyzed the oxidation of xylose with both NADP+ and NAD+ as cosubstrates with 10-fold higher affinity ...

journal_title：Journal of bacteriology

authors： Johnsen U,Schönheit P

更新日期：2004-09-01 00:00:00

abstract：:A cluster of Bacillus subtilis fatty acid synthetic genes was isolated by complementation of an Escherichia coli fabD mutant encoding a thermosensitive malonyl coenzyme A-acyl carrier protein transacylase. The B. subtilis genomic segment contains genes that encode three fatty acid synthetic proteins, malonyl coenzyme ...

journal_title：Journal of bacteriology

authors： Morbidoni HR,de Mendoza D,Cronan JE Jr

更新日期：1996-08-01 00:00:00

abstract：:The role of the proton gradient and external pH in the motility and chemotaxis of Bacillus subtilis was investigated. Presence of a substantial proton gradient is not necessary for motility or chemotaxis, as long as the electrical potential is sufficient to maintain motility. Changes in the proton gradient do, however...

journal_title：Journal of bacteriology

authors： Miller JB,Koshland DE Jr

更新日期：1980-01-01 00:00:00

abstract：:Lipopolysaccharide (LPS) was isolated from the unicellular blue-green bacterium Agmenellum quadruplicatum using the procedure of Westphal and Jann (1965). It was composed of a lipid A and polysaccharide region suggesting a similarity to other gram-negative LPSs. Chemical analyses demonstrated the presence of glucose, ...

journal_title：Journal of bacteriology

authors： Buttke TM,Ingram LO

更新日期：1975-12-01 00:00:00

abstract：:In response to molecular oxygen and/or fixed nitrogen, the product of the Klebsiella pneumoniae nitrogen fixation L (nifL) gene inhibits NifA-mediated transcriptional activation. Nitrogen regulation of NifL function occurs at two levels: transcription of the nifLA operon is regulated by the general Ntr system, and the...

journal_title：Journal of bacteriology

authors： He L,Soupene E,Kustu S

更新日期：1997-12-01 00:00:00

abstract：:A temperature-sensitive Schizosaccharomyces pombe mutant, cdc16-116, has been isolated which undergoes uncontrolled septation during its cell division cycle. The mutant accumulates two types of cells after 3 h of growth at the restrictive temperature: (i) type I cells (85% of the population), which complete nuclear di...

journal_title：Journal of bacteriology

authors： Minet M,Nurse P,Thuriaux P,Mitchison JM

更新日期：1979-01-01 00:00:00

abstract：:Reich, Paul R. (National Institutes of Health, Bethesda, Md.), Norman L. Somerson, James A. Rose, and Sherman M. Weissman. Genetic relatedness among mycoplasmas as determined by nucleic acid homology. J. Bacteriol. 91:153-160. 1966.-A sensitive membrane filter method to detect nucleic acid homology was used to determi...

journal_title：Journal of bacteriology

authors： Reich PR,Somerson NL,Rose JA,Weissman SM

更新日期：1966-01-01 00:00:00

abstract：:Twenty-four Lactobacillus plantarum supermotifs (LPSMs) with lengths from approximately 800 to 1,000 nucleotides were identified in the L. plantarum genome. LPSMs were conserved in other L. plantarum strains but not in other species. Secondary structure analysis predicted that LPSMs may fold into cruciform-like struct...

journal_title：Journal of bacteriology

authors： Wels M,Bongers RS,Boekhorst J,Molenaar D,Sturme M,de Vos WM,Siezen RJ,Kleerebezem M

更新日期：2009-05-01 00:00:00

abstract：:The gene encoding the fimbrial subunit protein of Bacteroides gingivalis 381, fimbrilin, has been cloned and sequenced. The gene was present as a single copy on the bacterial chromosome, and the codon usage in the gene conformed closely to that expected for an abundant protein. The predicted size of the mature protein...

journal_title：Journal of bacteriology

authors： Dickinson DP,Kubiniec MA,Yoshimura F,Genco RJ

更新日期：1988-04-01 00:00:00

abstract：:The homotrimeric enzyme Mt-Cam from Methanosarcina thermophila is the archetype of the gamma class of carbonic anhydrases. A search of databases queried with Mt-Cam revealed that a majority of the homologs comprise a putative subclass (CamH) in which there is major conservation of all of the residues essential for the...

journal_title：Journal of bacteriology

authors： Zimmerman SA,Tomb JF,Ferry JG

更新日期：2010-03-01 00:00:00

abstract：:2-Carboxybenzaldehyde dehydrogenase from the phenanthrene-degrading bacterium Nocardioides sp. strain KP7 was purified and characterized. The purified enzyme had a molecular mass of 53 kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and 205 kDa by gel filtration chromatography. Thus, the homotetramer ...

journal_title：Journal of bacteriology

authors： Iwabuchi T,Harayama S

更新日期：1997-10-01 00:00:00

abstract：:Aspartate transcarbamylase is synthesized during exponential growth of Bacillus subtilis and is inactivated when the cells enter the stationary phase. This work is a study of the regulation of aspartate transcarbamylase synthesis during growth and the stationary phase. Using specific immunoprecipitation of aspartate t...

journal_title：Journal of bacteriology

authors： Maurizi MR,Switzer RL

更新日期：1978-09-01 00:00:00

abstract：:Hydrolytic deamination of cytosine to uracil in DNA is increased in organisms adapted to high temperatures. Hitherto, the uracil base excision repair (BER) pathway has only been described in two archaeons, the crenarchaeon Pyrobaculum aerophilum and the euryarchaeon Archaeoglobus fulgidus, which are hyperthermophiles ...

journal_title：Journal of bacteriology

authors： Moen MN,Knævelsrud I,Haugland GT,Grøsvik K,Birkeland NK,Klungland A,Bjelland S

更新日期：2011-09-01 00:00:00

abstract：:A procedure is presented for the rapid screening of bacterial colonies to detect mutants unable to produce 14CO2 from a labeled precursor. The method is especially useful for mass screening for mutants that cannot be easily detected by their phenotypic characteristics. ...

journal_title：Journal of bacteriology

authors： Tabor H,Tabor CW,Hafner EW

更新日期：1976-10-01 00:00:00

abstract：:The DNAs of strains of three cyanobacterial genera (Anabaena, Plectonema, and Synechococcus) were found to be partially or fully resistant to many restriction endonucleases. This could be due to the absence of specific sequences or to modifications, rendering given sequences resistant to cleavage. The latter explanati...

journal_title：Journal of bacteriology

authors： Padhy RN,Hottat FG,Coene MM,Hoet PP

更新日期：1988-04-01 00:00:00

abstract：:Sequencing the insertion sites of 8,865 Tn917 insertions in Enterococcus faecalis strain OG1RF identified a hot spot in the replication terminus region corresponding to 6% of the genome where 65% of the transposons had inserted. In E. faecalis, Tn917 preferentially inserted at a 29-bp consensus sequence centered on TA...

journal_title：Journal of bacteriology

authors： Garsin DA,Urbach J,Huguet-Tapia JC,Peters JE,Ausubel FM

更新日期：2004-11-01 00:00:00

abstract：:The alginate lyase-encoding gene (algL) of Azotobacter chroococcum was localized to a 3.1-kb EcoRI DNA fragment that revealed an open reading frame of 1,116 bp. This open reading frame encodes a protein of 42.98 kDa, in agreement with the value previously reported by us for this protein. The deduced protein has a pote...

journal_title：Journal of bacteriology

authors： Peciña A,Pascual A,Paneque A

更新日期：1999-03-01 00:00:00

abstract：:Mutational analysis of the carboxy-terminal region of Escherichia coli HlyC was performed by site-directed mutagenesis. Replacement of residue Val-127 or Lys-129 reduced the activity of HlyC to about 30 or 60%, respectively, of that of the wild type, while replacement of Gly-128 reduced the activity to less than 1% of...

journal_title：Journal of bacteriology

authors： Guzmán-Verri C,García F,Arvidson S

更新日期：1997-09-01 00:00:00

abstract：:The region of plasmid NR1 concerned with resistance to Hg2+ and organomercurials consists of sequences found on restriction endonuclease fragments EcoRI-H and EcoRI-I. When both fragments were cloned together into a derivative of plasmid ColE1, the hybrid plasmid conferred properties indistinguishable from those of th...

journal_title：Journal of bacteriology

authors： Nakahara H,Silver S,Miki T,Rownd RH

更新日期：1979-10-01 00:00:00

abstract：:The Escherichia coli lacZ gene was used as a model system to identify specific sequence elements affecting mRNA stability. Various insertions and substitutions at the ribosome-binding site increased or decreased the rate of mRNA inactivation by up to fourfold. Deletion of a dyad symmetry, which may give rise to a very...

journal_title：Journal of bacteriology

authors： Petersen C

更新日期：1991-04-01 00:00:00

abstract：:Gene III (gIII) of phiLf, a filamentous phage specifically infecting Xanthomonas campestris pv. campestris, was previously shown to encode a virion-associated protein (pIII) required for phage adsorption. In this study, the transcription start site for the gene and the N-terminal sequence of the protein were determine...

journal_title：Journal of bacteriology

authors： Lin NT,Liu TJ,Lee TC,You BY,Yang MH,Wen FS,Tseng YH

更新日期：1999-04-01 00:00:00

abstract：:Certain group VIIIB transition metal compounds were found to inhibit cell division in Escherichia coli, causing marked filamentous growth. Gram-negative bacilli were the most sensitive to this effect, whereas gram-positive bacilli responded only at near-toxic levels of the metal. None of the cocci tested showed any ap...

journal_title：Journal of bacteriology

authors： Rosenberg B,Renshaw E,Vancamp L,Hartwick J,Drobnik J

更新日期：1967-02-01 00:00:00

abstract：:The opportunistic pathogen Pseudomonas aeruginosa utilizes a type III secretion system (T3SS) to evade phagocytosis and damage eukaryotic cells. Transcription of the T3SS regulon is controlled by ExsA, a member of the AraC/XylS family of transcriptional regulators. These family members generally consist of an approxim...

journal_title：Journal of bacteriology

authors： Brutinel ED,Vakulskas CA,Yahr TL

更新日期：2009-06-01 00:00:00

abstract：:A lipoteichoic acid (LTA) was extracted from Listeria monocytogenes (serotype 1) by phenol-water partition and isolated by gel-filtration chromatography. The LTA exhibited amphiphilic properties by changes in gel-filtration mobility in the presence of detergent buffers and after mild base hydrolysis. In a hemagglutina...

journal_title：Journal of bacteriology

authors： Hether NW,Jackson LL

更新日期：1983-11-01 00:00:00