Abstract:
:Mutational analysis of the carboxy-terminal region of Escherichia coli HlyC was performed by site-directed mutagenesis. Replacement of residue Val-127 or Lys-129 reduced the activity of HlyC to about 30 or 60%, respectively, of that of the wild type, while replacement of Gly-128 reduced the activity to less than 1% of the wild-type level. Complete inactivation of HlyC was caused by a double mutation, replacement of Gly-128 with valine and of Lys-129 with isoleucine. Analysis of culture supernatants from mutants with reduced hemolytic activity by two-dimensional gel electrophoresis revealed the production and simultaneous secretion of nonacylated, monoacylated, and fully acylated HlyA forms, demonstrating impairment of the acylation reaction, possibly due to a decreased affinity of HlyC for the individual HlyA acylation sites.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Guzmán-Verri C,García F,Arvidson Sdoi
10.1128/jb.179.18.5959-5962.1997subject
Has Abstractpub_date
1997-09-01 00:00:00pages
5959-62issue
18eissn
0021-9193issn
1098-5530journal_volume
179pub_type
杂志文章abstract::A 12.6-kb DNA fragment from Streptomyces glaucescens GLA.0 containing the 12 genes for tetracenomycin (TCM) C biosynthesis and resistance enabled Streptomyces lividans to produce TCM C. Transcriptional analysis of the tcmPG intergenic region in this cluster established the presence of two divergent promoters. The tcmI...
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journal_title:Journal of bacteriology
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.6.2843-2846.1987
更新日期:1987-06-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.115.3.1103-1107.1973
更新日期:1973-09-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.150.1.122-131.1982
更新日期:1982-04-01 00:00:00
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journal_title:Journal of bacteriology
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doi:10.1128/jb.179.3.919-927.1997
更新日期:1997-02-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.96.3.706-712.1968
更新日期:1968-09-01 00:00:00
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journal_title:Journal of bacteriology
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doi:10.1128/JB.137.1.461-468.1979
更新日期:1979-01-01 00:00:00
abstract::The ligated loop of the rabbit intestine was investigated as a possible experimental model for the study of Clostridium perfringens food poisoning. The method of preparation of the challenge inoculum was important in determining whether a given strain would provoke a response. When cultures were grown for 4 hr at 37 C...
journal_title:Journal of bacteriology
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doi:10.1128/JB.95.5.1560-1566.1968
更新日期:1968-05-01 00:00:00
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更新日期:1980-05-01 00:00:00
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journal_title:Journal of bacteriology
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.12.4023-4029.1997
更新日期:1997-06-01 00:00:00
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journal_title:Journal of bacteriology
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doi:10.1128/JB.99.2.389-394.1969
更新日期:1969-08-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2002-09-01 00:00:00
abstract::The pink yeast Rhodotorula rubra of marine origin was found to be capable of extended growth at very low phosphate concentrations (K(0.5) = 10.8 nm). Average intracellular phosphate concentrations, based on isotope exchange techniques, were 15 to 200 nm, giving concentration gradients across the cell envelope of about...
journal_title:Journal of bacteriology
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doi:10.1128/JB.113.2.599-611.1973
更新日期:1973-02-01 00:00:00
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journal_title:Journal of bacteriology
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更新日期:1976-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2005-03-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2002-09-01 00:00:00
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journal_title:Journal of bacteriology
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更新日期:1984-05-01 00:00:00
abstract::The UspA1 and UspA2 proteins of Moraxella catarrhalis are structurally related, are exposed on the bacterial cell surface, and migrate as very high-molecular-weight complexes in sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Previous analysis of uspA1 and uspA2 mutants of M. catarrhalis strain 035E indicat...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.5.1364-1373.2000
更新日期:2000-03-01 00:00:00