Abstract:
:A mutant (designated mec(-)) of Escherichia coli F(+) 100 endo I(-)su(+) r(K) (-)m(K) (+) has been isolated which is defective in cytosine-specific deoxyribonucleic acid (DNA) methylase activity. The DNA of this mutant, as well as the DNA of phages lambda and fd propagated in it, is virtually devoid of 5-methyl-cytosine (MeC); in contrast, the mutation has no significant effect on the level of N(6)-methyladenine in DNA. Phage lambda grown on the mec(-) mutant is more strongly restricted by N-3-containing cells than is lambda grown on the mec(+) parent. These results suggest that methylation of certain cytosine residues by the E. coli K-12 enzyme partially protects lambda DNA from either the N-3 restriction nuclease or against secondary degradation subsequent to N-3-specific degradation. Analysis of the MeC level in viral and cellular DNA obtained from mec(+), mec(+) (m(N3) (+)), and mec(-) (m(N3) (+)) strains has led to the conclusion that the R-factor controlled DNA-cytosine methylase may be capable of methylating a sequence(s) which is a substrate for the K-12 enzyme.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Hattman S,Schlagman S,Cousens Ldoi
10.1128/JB.115.3.1103-1107.1973subject
Has Abstractpub_date
1973-09-01 00:00:00pages
1103-7issue
3eissn
0021-9193issn
1098-5530journal_volume
115pub_type
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.122.3.1394-1395.1975
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journal_title:Journal of bacteriology
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更新日期:1962-05-01 00:00:00
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更新日期:2010-02-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.166.1.38-43.1986
更新日期:1986-04-01 00:00:00
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更新日期:1977-05-01 00:00:00
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pub_type: 杂志文章
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更新日期:2000-06-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.2.303-316.1998
更新日期:1998-01-01 00:00:00
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pub_type: 杂志文章
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.188.3.1205-1210.2006
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.167.1.407-410.1986
更新日期:1986-07-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2003-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1961-12-01 00:00:00
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pub_type: 杂志文章
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更新日期:1972-11-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1988-03-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.151.2.777-782.1982
更新日期:1982-08-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2011-02-01 00:00:00
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pub_type: 杂志文章
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更新日期:1980-07-01 00:00:00
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pub_type: 杂志文章
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更新日期:1992-12-01 00:00:00
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更新日期:2012-04-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.149.2.426-433.1982
更新日期:1982-02-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.23.7727-7731.1993
更新日期:1993-12-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2002-01-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.159.2.805-807.1984
更新日期:1984-08-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1967-10-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JB.136.1.414-418.1978
更新日期:1978-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2010-05-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.87.3.690-695.1964
更新日期:1964-03-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.4.1831-1836.1988
更新日期:1988-04-01 00:00:00