Characterization of Pseudomonas aeruginosa derepressed R-plasmids.

Abstract:

:A genetic study of conjugal transmissibility of two R-plasmids was undertaken in Pseudomonas aeruginosa. Conjugally derepressed mutants of the R-plasmids were isolated, and examination of 11 independent mutants revealed that 10 were recessive to the wild-type transfer repressor, whereas 1 mutant was cis dominant. Cross-repression was observed between the two R-plasmids, suggesting that they have functionally equivalent systems for regulating the expression of tra loci. The derepressed R-plasmid mutants exhibited several characteristics, in addition to derepressed transfer, that were not expressed by the parental plasmids. These included sensitivity to certain donor-specific phages, inhibition of multiplication of a transducing phage, and, in the one case examined, a high degree of entry exclusion. The coexpression of these different functions suggests that their respective genetic loci are controlled by the same regulatory system as that of tra, or else that they are part of the tra complex.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Chandler PM,Krishnapillai V

doi

10.1128/JB.130.2.596-603.1977

keywords:

subject

Has Abstract

pub_date

1977-05-01 00:00:00

pages

596-603

issue

2

eissn

0021-9193

issn

1098-5530

journal_volume

130

pub_type

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