Abstract:
:Investigation of the chromosomal region downstream of the lacZ gene from Lactobacillus delbrueckii subsp. bulgaricus revealed the presence of a gene (prtB) encoding a proteinase of 1,946 residues with a predicted molecular mass of 212 kDa. The deduced amino acid sequence showed that PrtB proteinase displays significant homology with the N termini and catalytic domains of lactococcal PrtP cell surface proteinases and is probably synthesized as a preproprotein. However, the presence of a cysteine near the histidine of the PrtB active site suggests that PrtB belongs to the subfamily of cysteine subtilisins. The C-terminal region strongly differs from those of PrtP proteinases by having a high lysine content, an imperfect duplication of 41 residues, and a degenerated sequence compared with the consensus sequence for proteins anchoring in the cell walls of gram-positive bacteria. Finally, the product of the truncated prtM-like gene located immediately upstream of the prtB gene seems too short to be involved in the maturation of PrtB.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Gilbert C,Atlan D,Blanc B,Portailer R,Germond JE,Lapierre L,Mollet Bdoi
10.1128/jb.178.11.3059-3065.1996subject
Has Abstractpub_date
1996-06-01 00:00:00pages
3059-65issue
11eissn
0021-9193issn
1098-5530journal_volume
178pub_type
杂志文章abstract::The DnaK protein of Zymomonas mobilis (DnaKz) was identified and found to be 80% identical to the DnaK protein of Escherichia coli on the basis of the sequence of the N-terminal 21 amino acids. The dnaKz gene was cloned and found to be expressed in a thermosensitive dnaK mutant of Escherichia coli. Expression of the f...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.10.3228-3231.1993
更新日期:1993-05-01 00:00:00
abstract::Streptococcus faecalis transposon Tn917 was cloned in Escherichia coli on plasmid vector pBR325. The erythromycin resistance determinant of Tn917 was not expressed in the E. coli background. The nucleotide sequence of Tn917 was determined and found to be 5,257 base pairs in length. Six open reading frames (ORFs) were ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.164.2.782-796.1985
更新日期:1985-11-01 00:00:00
abstract::A mutant (pgi) of Coprinus macrorhizus deficient in phosphoglucose isomerase did not grow on fructose and grew poorly on glucose. The pgi mutation inhibited the formation of monokaryotic and dikaryotic fruiting bodies. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.144.1.432-434.1980
更新日期:1980-10-01 00:00:00
abstract::tRNAs play a critical role in mRNA decoding, and posttranscriptional modifications within tRNAs drive decoding efficiency and accuracy. The types and positions of tRNA modifications in model bacteria have been extensively studied, and tRNA modifications in a few eukaryotic organisms have also been characterized and lo...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00690-18
更新日期:2019-04-09 00:00:00
abstract::To examine as randomly as possible the role of the beta-ketoacyl and acyl carrier protein (ACP) components of bacterial type II polyketide synthases (PKSs), homologs of the chain-length-factor (CLF) genes were cloned from the environmental community of microorganisms. With PCR primers derived from conserved regions of...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.23.7360-7368.1997
更新日期:1997-12-01 00:00:00
abstract::MurG is a peripheral membrane protein that is one of the key enzymes in peptidoglycan biosynthesis. The crystal structure of Escherichia coli MurG (S. Ha, D. Walker, Y. Shi, and S. Walker, Protein Sci. 9:1045-1052, 2000) contains a hydrophobic patch surrounded by basic residues that may represent a membrane associatio...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.13.3773-3779.2003
更新日期:2003-07-01 00:00:00
abstract::A 7.5-kbp fragment of chromosomal DNA downstream of the Vibrio cholerae vibriobactin outer membrane receptor, viuA, and the vibriobactin utilization gene, viuB, was recovered from a Sau3A lambda library of O395 chromosomal DNA. By analogy with the genetic organization of the Escherichia coli enterobactin gene cluster,...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.6.1731-1738.2000
更新日期:2000-03-01 00:00:00
abstract::Catabolism of nonphenolic arenes is frequently initiated by dioxygenases, yielding single isomer products with two adjacent hydroxylated asymmetric centers. The next enzymic reaction dehydrogenates these cyclic cis-diols, with aromatization yielding catechols for ring cleavage. There are two stereochemical questions t...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.12.4023-4029.1997
更新日期:1997-06-01 00:00:00
abstract::The colicin E2 immunity (ceiB) and lysis (celB) genes of colicin plasmid ColE2-P9 were cloned as a 900-base-pair insert under the control of the lac promoter in high-copy-number plasmid pUR222. Hosts carrying this plasmid were immune to colicin E2, produced increased amounts of immunity protein (molecular weight, 9,00...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.156.1.109-114.1983
更新日期:1983-10-01 00:00:00
abstract::The glpD (MSMEG_6761) gene encoding glycerol-3-phosphate dehydrogenase was shown to be crucial for M. smegmatis to utilize glycerol as the sole carbon source. The glpD gene likely forms the glpFKD operon together with glpF and glpK, encoding a glycerol facilitator and glycerol kinase, respectively. The gylR (MSMEG_675...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00511-19
更新日期:2019-11-20 00:00:00
abstract::Elevation of active sigma(E) levels in Escherichia coli by either repressing the expression of rseA encoding an anti-sigma(E) factor or cloning rpoE in a multicopy plasmid, led to a large decrease in the number of dead cells and the accumulation of cellular proteins in the medium in the stationary phase. The numbers o...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.18.5231-5237.2000
更新日期:2000-09-01 00:00:00
abstract::A combined physical and genetic map of the genome of strain SG24 of Vibrio cholerae O139 Bengal, a novel non-O1 strain having epidemic potential, has been constructed by using the enzymes NotI, SfiI, and CeuI. The genome of SG24 is circular, and the genome size is about 3. 57 Mb. The linkages between 47 NotI and 32 Sf...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.17.4516-4522.1998
更新日期:1998-09-01 00:00:00
abstract::Cells of the cyanobacterium Anabaena variabilis starved for phosphate for 3 days took up phosphate at about 100 times the rate of unstarved cells. Kinetic data suggested that a new transport system had been induced by starvation for phosphate. The inducible phosphate transport system was quickly repressed by addition ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.3.1143-1147.1988
更新日期:1988-03-01 00:00:00
abstract::Mycobacterium tuberculosis can persist for many years within host lung tissue without causing clinical disease. Little is known about the state in which the bacilli survive, although it is frequently referred to as dormancy. Some evidence suggests that cells survive in nutrient-deprived stationary phase. Therefore, we...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.1.270-283.1999
更新日期:1999-01-01 00:00:00
abstract::Escherichia coli produces linoleic acid in the late stationary phase. This was the case whether the cultures were grown aerobically or anaerobically on a supplemented glucose-salts medium. The linoleic acid was detected by thin-layer chromatography and was measured as the methyl ester by gas chromatography. The linole...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.17.5324-5328.1993
更新日期:1993-09-01 00:00:00
abstract::The gene encoding a 45-kDa protein (45K) of Chlamydia trachomatis serovar F was cloned, sequenced, and overexpressed in Escherichia coli. Alignment of the deduced peptide sequence with E. coli elongation factor Tu (EF-Tu) demonstrated 69% identity. The 45K was recognized by a Chlamydia genus-specific monoclonal antibo...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.4.1184-1187.1994
更新日期:1994-02-01 00:00:00
abstract::Massetolide A is a cyclic lipopeptide (CLP) antibiotic produced by various Pseudomonas strains from diverse environments. Cloning, sequencing, site-directed mutagenesis, and complementation showed that massetolide A biosynthesis in P. fluorescens SS101 is governed by three nonribosomal peptide synthetase (NRPS) genes,...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01563-07
更新日期:2008-04-01 00:00:00
abstract::Methionine sulfoxide reductase (Msr) catalyzes the thioredoxin-dependent reduction and repair of methionine sulfoxide (MetO). Although Msr genes are not present in most hyperthermophile genomes, an Msr homolog encoding an MsrA-MsrB fusion protein (MsrAB(Tk)) was present on the genome of the hyperthermophilic archaeon ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00751-06
更新日期:2007-10-01 00:00:00
abstract::Lornitzo, Frank A. (Veterans Administration Hospital, Madison, Wis.), and Dexter S. Goldman. Reversible effect of bicarbonate on the inhibition of mycobacterial and yeast transglucosylases by mycoribnin. J. Bacteriol. 89:1086-1091. 1965.-The transglucosylase which catalyzes the formation of trehalose-6-phosphate from ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.89.4.1086-1091.1965
更新日期:1965-04-01 00:00:00
abstract::Proteus rettgeri and Escherichia coli W were shown to express structurally different penicillin G acylases. The enzymes had similar substrate specificity but differed in molecular weight, isoelectric point, and electrophoretic mobility in polyacrylamide gels and did not antigenically cross-react. When the organisms we...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.163.3.925-932.1985
更新日期:1985-09-01 00:00:00
abstract::The bacterioferritin (BFR) of Escherichia coli K-12 is an iron-storage hemoprotein, previously identified as cytochrome b1. The bacterioferritin gene (bfr) has been cloned, sequenced, and located in the E. coli linkage map. Initially a gene fusion encoding a BFR-lambda hybrid protein (Mr 21,000) was detected by immuno...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.7.3940-3947.1989
更新日期:1989-07-01 00:00:00
abstract::The growth of Bacillus subtilis mutant betaA177 can be inhibited under special conditions in which not enough autolytic enzymes are produced for optimal growth. Electron microscopy studies show that during growth inhibition there is localized thickening of the cell wall at positions where cells bend. A model is propos...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.109.3.1247-1257.1972
更新日期:1972-03-01 00:00:00
abstract::A spontaneously arising regulatory mutant of the gluconate system in Escherichia coli was isolated. This mutant became constitutive, probably in one step, for gluconate high-affinity transport, gluconokinase, and gluconate-6-P dehydrase. The mutation involved (gntR18) is cotransducible with asd. Pseudorevertants, deri...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.114.2.469-473.1973
更新日期:1973-05-01 00:00:00
abstract::Spo0A is a DNA binding protein in Bacillus subtilis required for the activation of spoIIG and other promoters at the onset of endospore formation. Activation of some of these promoters may involve interaction of Spo0A and the sigmaA subunit of RNA polymerase. Previous studies identified two single-amino-acid substitut...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.14.3578-3583.1998
更新日期:1998-07-01 00:00:00
abstract::We identified several promoters responsible for the expression of regA, which encodes a global virulence regulator in Citrobacter rodentium. Expression of some of the promoters was strongly autoactivated by RegA in conjunction with bicarbonate. Biochemical and mutational analyses were used to determine the consensus s...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01224-10
更新日期:2011-04-01 00:00:00
abstract::Three spontaneous derivatives of Streptococcus faecium ATCC 9790, originally isolated as conditionally Triton X-100 detergent-resistant at 25 degrees C, displayed normal penicillin-induced rates of lysis at 37 degrees C but substantially reduced rates of lysis and killing at 25 degrees C. The addition of exogenous uns...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.159.2.805-807.1984
更新日期:1984-08-01 00:00:00
abstract::FpvR is a presumed cytoplasmic membrane-associated anti-sigma factor that controls the activities of extracytoplasmic function sigma factors PvdS and FpvI responsible for transcription of pyoverdine biosynthetic genes and the ferric pyoverdine receptor gene, fpvA, respectively. Using deletion analysis and an in vivo b...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.16.5648-5657.2005
更新日期:2005-08-01 00:00:00
abstract::The function of SufI, a well-studied substrate of the TatABC translocase in Escherichia coli, is not known. It was earlier implicated in cell division, based on the finding that multiple copies of sufI suppressed the phenotypes of cells with mutations in ftsI (ftsI23), which encodes a divisomal transpeptidase. Recentl...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00773-07
更新日期:2007-11-01 00:00:00
abstract::Pseudomonas savastanoi pv. savastanoi strains harbor native plasmids belonging to the pPT23A plasmid family (PFPs) which are detected in all pathovars of the related species Pseudomonas syringae examined and contribute to the ecological and pathogenic fitness of their host. However, there is a general lack of informat...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01067-07
更新日期:2008-01-01 00:00:00
abstract::The par genes of unit-copy plasmids P1 and F promote equitable distribution of plasmid copies to daughter cells and can be considered to be functional analogs of eucaryotic centromeres. Composite plasmids were constructed which carry either two functional P1 par regions or one F and one P1 region. Unlike dicentric chr...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.158.2.742-745.1984
更新日期:1984-05-01 00:00:00