Ultrastructural studies on a mutant of Bacillus subtilis whose growth is inhibited due to insufficient autolysin production.

Abstract:

:The growth of Bacillus subtilis mutant betaA177 can be inhibited under special conditions in which not enough autolytic enzymes are produced for optimal growth. Electron microscopy studies show that during growth inhibition there is localized thickening of the cell wall at positions where cells bend. A model is proposed to explain this result. Rapid growth can be restored by adding lysozyme or a B. subtilis autolysin mixture to a growth-inhibited betaA177 culture. Such addition reduces the localized wall thickening and causes other changes in surface morphology which are described and discussed. Septum formation seems to be relatively less inhibited than cell elongation when lytic enzyme levels are reduced. Measurements were made demonstrating that walls at ends of cells are morphologically different from walls at sides of cells in cultures of betaA177 growing at 51 C.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Fan DP,Beckman MM,Cunningham WP

doi

10.1128/JB.109.3.1247-1257.1972

subject

Has Abstract

pub_date

1972-03-01 00:00:00

pages

1247-57

issue

3

eissn

0021-9193

issn

1098-5530

journal_volume

109

pub_type

杂志文章
  • Borrelia burgdorferi bba74 is expressed exclusively during tick feeding and is regulated by both arthropod- and mammalian host-specific signals.

    abstract::Although BBA74 initially was described as a 28-kDa virulence-associated outer-membrane-spanning protein with porin-like function, subsequent studies revealed that it is periplasmic and downregulated in mammalian host-adapted spirochetes. To further elucidate the role of this protein in the Borrelia burgdorferi tick-ma...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01802-08

    authors: Mulay VB,Caimano MJ,Iyer R,Dunham-Ems S,Liveris D,Petzke MM,Schwartz I,Radolf JD

    更新日期:2009-04-01 00:00:00

  • Insertion of transposons through the major cotransduction gap of Escherichia coli K-12.

    abstract::The major cotransduction gap of the Escherichia coli chromosome extends from mini 31 to 34. We have inserted transposons through this gap which, by sequential transduction, link sbcA (min 29.8) with manA (min 35.7) and thus eliminate the gap. These results indicate that the length of DNA in the region, as measured by ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.149.1.106-113.1982

    authors: Fouts KE,Barbour SD

    更新日期:1982-01-01 00:00:00

  • Tyrosine 106 of CheY plays an important role in chemotaxis signal transduction in Escherichia coli.

    abstract::CheY is the response regulator in the signal transduction pathway of bacterial chemotaxis. Position 106 of CheY is occupied by a conserved aromatic residue (tyrosine or phenylalanine) in the response regulator superfamily. A number of substitutions at position 106 have been made and characterized by both behavioral an...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.14.4208-4215.1996

    authors: Zhu X,Amsler CD,Volz K,Matsumura P

    更新日期:1996-07-01 00:00:00

  • Experimental observations consistent with a surface tension model of gliding motility of Myxococcus xanthus.

    abstract::We have presented experimental evidence to support the model that gliding motility of Myxococcus xanthus is driven by surface tension. (i) Motility is inhibited by the addition of sufficient exogenous, nontoxic surfactants to swamp out the cells' own surfactant gradient. (ii) M. xanthus does not move polystyrene latex...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.155.3.1367-1371.1983

    authors: Dworkin M,Keller KH,Weisberg D

    更新日期:1983-09-01 00:00:00

  • Conjugative plasmids in Neisseria gonorrhoeae.

    abstract::A conjugation system initially discovered in beta-lactamase-producing gonococci mobilized small non-selftransmissible R plasmids encoding beta-lactamase (penicillinase) production into other gonococci, Neisseria, and Escherichia coli. This conjugation system was mediated by a separate selftransmissible plasmid of 23.9...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.134.1.278-286.1978

    authors: Sox TE,Mohammed W,Blackman E,Biswas G,Sparling PF

    更新日期:1978-04-01 00:00:00

  • Outer membrane proteins of Escherichia coli. VII. Evidence that bacteriophage-directed protein 2 functions as a pore.

    abstract::Protein 1, a major protein of the outer membrane of Escherichia coli, has been shown to be the pore allowing the passage of small hydrophilic solutes across the outer membrane. In E. coli K-12 protein 1 consists of two subspecies, 1a and 1b, whereas in E. coli B it consists of a single species which has an electrophor...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.133.3.1181-1189.1978

    authors: Pugsley AP,Schnaitman CA

    更新日期:1978-03-01 00:00:00

  • Interaction of Copper Toxicity and Oxidative Stress in Campylobacter jejuni.

    abstract::Copper is both a required micronutrient and a source of toxicity in most organisms, including Campylobacter jejuni Two proteins expressed in C. jejuni (termed CopA and CueO) have been shown to be a copper transporter and multicopper oxidase, respectively. We have isolated strains with mutations in these genes, and her...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00208-18

    authors: Gardner SP,Olson JW

    更新日期:2018-10-10 00:00:00

  • A cytochrome cbb3 (cytochrome c) terminal oxidase in Azospirillum brasilense Sp7 supports microaerobic growth.

    abstract::Spectral analysis indicated the presence of a cytochrome cbb3 oxidase under microaerobic conditions in Azospirillum brasilense Sp7 cells. The corresponding genes (cytNOQP) were isolated by using PCR. These genes are organized in an operon, preceded by a putative anaerobox. The phenotype of an A. brasilense cytN mutant...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.21.5689-5696.1998

    authors: Marchal K,Sun J,Keijers V,Haaker H,Vanderleyden J

    更新日期:1998-11-01 00:00:00

  • Induction of pigmentation in nonproliferating cells of Serratia marcescens by addition of single amino acids.

    abstract::Addition of casein hydrolysate to suspensions of washed, nonpigmented, nonproliferating Serratia marcescens incubating at 27 C induced biosynthesis of prodigiosin. Four amino acids of casein hydrolysate, dl-aspartic acid, l-glutamic acid, l-proline, and l-alanine caused formation of pigment when added individually. dl...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.106.2.444-448.1971

    authors: Williams RP,Gott CL,Qadri SM

    更新日期:1971-05-01 00:00:00

  • Limited role for the DsrA and RprA regulatory RNAs in rpoS regulation in Salmonella enterica.

    abstract::RpoS, the sigma factor of enteric bacteria that responds to stress and stationary phase, is subject to complex regulation acting at multiple levels, including transcription, translation, and proteolysis. Increased translation of rpoS mRNA during growth at low temperature, after osmotic challenge, or with a constitutiv...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00206-06

    authors: Jones AM,Goodwill AG,Elliott T

    更新日期:2006-07-01 00:00:00

  • Identification of the PhoB Regulon and Role of PhoU in the Phosphate Starvation Response of Caulobacter crescentus.

    abstract:UNLABELLED:An ability to sense and respond to changes in extracellular phosphate is critical for the survival of most bacteria. For Caulobacter crescentus, which typically lives in phosphate-limited environments, this process is especially crucial. Like many bacteria, Caulobacter responds to phosphate limitation throug...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00658-15

    authors: Lubin EA,Henry JT,Fiebig A,Crosson S,Laub MT

    更新日期:2015-10-19 00:00:00

  • Adaptation of Xanthobacter autotrophicus GJ10 to bromoacetate due to activation and mobilization of the haloacetate dehalogenase gene by insertion element IS1247.

    abstract::Monobromoacetate (MBA) is toxic for the 1,2-dichloroethane-degrading bacterium Xanthobacter autotrophicus GJ10 at concentrations higher than 5 mM. Mutants which are able to grow on higher concentrations of MBA were isolated and found to overexpress haloacid dehalogenase, which is encoded by the dhlB gene. In mutant GJ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.5.1348-1356.1995

    authors: van der Ploeg J,Willemsen M,van Hall G,Janssen DB

    更新日期:1995-03-01 00:00:00

  • A topological model for the general aromatic amino acid permease, AroP, of Escherichia coli.

    abstract::The general aromatic amino acid permease, AroP, of Escherichia coli is responsible for the active transport of phenylalanine, tyrosine, and tryptophan. A proposed topological model for the AroP permease, consisting of 12 hydrophobic transmembrane spans connected by hydrophilic loops, is very similar to that of the clo...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.10.3317-3323.1997

    authors: Cosgriff AJ,Pittard AJ

    更新日期:1997-05-01 00:00:00

  • Role of transmembrane pH gradient and membrane binding in nisin pore formation.

    abstract::Nisin is a cationic antimicrobial peptide that belongs to the group of lantibiotics. It is thought to form oligomeric pores in the target membrane by a mechanism that requires the transmembrane electrical potential delta psi and that involves local pertubation of the lipid bilayer structure. Here we show that nisin do...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.1.135-140.1997

    authors: Moll GN,Clark J,Chan WC,Bycroft BW,Roberts GC,Konings WN,Driessen AJ

    更新日期:1997-01-01 00:00:00

  • Sigma factor RpoS controls alkylresorcinol synthesis through ArpR, a LysR-type regulatory protein, during encystment of Azotobacter vinelandii.

    abstract::Azotobacter vinelandii is a bacterium which undergoes a differentiation process leading to the formation of metabolically dormant cysts. During the encystment process, A. vinelandii produces alkylresorcinol lipids (ARs) that replace the membrane phospholipids and are also components of the layers covering the cyst. Th...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01946-12

    authors: Romero Y,Moreno S,Guzmán J,Espín G,Segura D

    更新日期:2013-04-01 00:00:00

  • Identification and overexpression in Escherichia coli of a Mycobacterium leprae gene, pon1, encoding a high-molecular-mass class A penicillin-binding protein, PBP1.

    abstract::Cosmid B577, a member of the collection of ordered clones corresponding to the genome of Mycobacterium leprae, contains a gene, provisionally called pon1, that encodes an 821-amino-acid-residue high-molecular-mass class A penicillin-binding protein, provisionally called PBP1. With similar amino acid sequences and modu...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.6.1707-1711.1996

    authors: Basu J,Mahapatra S,Kundu M,Mukhopadhyay S,Nguyen-Distèche M,Dubois P,Joris B,Van Beeumen J,Cole ST,Chakrabarti P,Ghuysen JM

    更新日期:1996-03-01 00:00:00

  • Propanediol utilization genes (pdu) of Salmonella typhimurium: three genes for the propanediol dehydratase.

    abstract::The propanediol utilization (pdu) operon of Salmonella typhimurium encodes proteins required for the catabolism of propanediol, including a coenzyme B12-dependent propanediol dehydratase. A clone that expresses propanediol dehydratase activity was isolated from a Salmonella genomic library. DNA sequence analysis showe...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.21.6633-6639.1997

    authors: Bobik TA,Xu Y,Jeter RM,Otto KE,Roth JR

    更新日期:1997-11-01 00:00:00

  • Anaerobic electron transport in anaerobic flagellum formation in Escherichia coli.

    abstract::Flagellum formation by ubiquinone- and menaquinone-deficient mutant strains of Escherichia coli K-12 was studied under both aerobic and anaerobic growth conditions. Ubiquinone was found to be obligatory for aerobic flagellum formation but could be replaced by menaquinone for anaerobic flagellum formation. A mutant dev...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.132.3.1034-1035.1977

    authors: Hertz R,Bar-Tana J

    更新日期:1977-12-01 00:00:00

  • Expressed genes for plant-type ribulose 1,5-bisphosphate carboxylase/oxygenase in the photosynthetic bacterium Chromatium vinosum, which possesses two complete sets of the genes.

    abstract::Two sets of genes for the large and small subunits of ribulose 1,5-bisphosphate carboxylase/oxygenase (RuBisCO) were detected in the photosynthetic purple sulfur bacterium Chromatium vinosum by hybridization analysis with RuBisCO gene probes, cloned by using the lambda Fix vector, and designated rbcL-rbcS and rbcA-rbc...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.5.2391-2400.1989

    authors: Viale AM,Kobayashi H,Akazawa T

    更新日期:1989-05-01 00:00:00

  • Identification of a novel operon in Lactococcus lactis encoding three enzymes for lactic acid synthesis: phosphofructokinase, pyruvate kinase, and lactate dehydrogenase.

    abstract::The discovery of a novel multicistronic operon that encodes phosphofructokinase, pyruvate kinase, and lactate dehydrogenase in the lactic acid bacterium Lactococcus lactis is reported. The three genes in the operon, designated pfk, pyk, and ldh, contain 340, 502, and 325 codons, respectively. The intergenic distances ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.9.2541-2551.1993

    authors: Llanos RM,Harris CJ,Hillier AJ,Davidson BE

    更新日期:1993-05-01 00:00:00

  • Conditional synthesis and utilization of 1,5-anhydroglucitol in Escherichia coli.

    abstract::A cyclic polyol, 1,5-anhydro-D-glucitol (AG), is widely detected in most organisms, although little is known about its metabolism and physiological roles. The present study demonstrates the synthesis of AG in Escherichia coli C600. The major portion of the synthesized AG was indicated to be derived from glucose retain...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.22.7138-7141.1993

    authors: Shiga Y,Mizuno H,Akanuma H

    更新日期:1993-11-01 00:00:00

  • UDP-glucuronic acid decarboxylases of Bacteroides fragilis and their prevalence in bacteria.

    abstract::Xylose is rarely described as a component of bacterial glycans. UDP-xylose is the nucleotide-activated form necessary for incorporation of xylose into glycans and is synthesized by the decarboxylation of UDP-glucuronic acid (UDP-GlcA). Enzymes with UDP-GlcA decarboxylase activity include those that lead to the formati...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.05337-11

    authors: Coyne MJ,Fletcher CM,Reinap B,Comstock LE

    更新日期:2011-10-01 00:00:00

  • Nucleotide sequences of the gnd genes from nine natural isolates of Escherichia coli: evidence of intragenic recombination as a contributing factor in the evolution of the polymorphic gnd locus.

    abstract::Nine natural isolates of Escherichia coli were examined, and the sequence of the entire 1,404 bases of the gnd gene (6-phosphogluconate dehydrogenase, EC 1.1.1.44) was determined. These isolates, along with E. coli K-12, constitute 10 strains for analysis. (The sequence of the E. coli K-12 gnd gene is known.) A total ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.12.3894-3900.1991

    authors: Bisercić M,Feutrier JY,Reeves PR

    更新日期:1991-06-01 00:00:00

  • An n-alkane-responsive promoter element found in the gene encoding the peroxisomal protein of Candida tropicalis does not contain a C(6) zinc cluster DNA-binding motif.

    abstract::When an asporogenic diploid yeast, Candida tropicalis, is cultivated on n-alkane, the expression of the genes encoding enzymes of the peroxisomal beta-oxidation pathway is highly induced. An upstream activation sequence (UAS) which can induce transcription in response to n-alkane (UAS(ALK)) was identified on the promo...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.182.9.2492-2497.2000

    authors: Kanai T,Hara A,Kanayama N,Ueda M,Tanaka A

    更新日期:2000-05-01 00:00:00

  • Substrate specificity of naphthalene dioxygenase: effect of specific amino acids at the active site of the enzyme.

    abstract::The three-component naphthalene dioxygenase (NDO) enzyme system carries out the first step in the aerobic degradation of naphthalene by Pseudomonas sp. strain NCIB 9816-4. The three-dimensional structure of NDO revealed that several of the amino acids at the active site of the oxygenase are hydrophobic, which is consi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.182.6.1641-1649.2000

    authors: Parales RE,Lee K,Resnick SM,Jiang H,Lessner DJ,Gibson DT

    更新日期:2000-03-01 00:00:00

  • HexR Controls Glucose-Responsive Genes and Central Carbon Metabolism in Neisseria meningitidis.

    abstract:UNLABELLED:Neisseria meningitidis, an exclusively human pathogen and the leading cause of bacterial meningitis, must adapt to different host niches during human infection. N. meningitidis can utilize a restricted range of carbon sources, including lactate, glucose, and pyruvate, whose concentrations vary in host niches...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00659-15

    authors: Antunes A,Golfieri G,Ferlicca F,Giuliani MM,Scarlato V,Delany I

    更新日期:2015-12-07 00:00:00

  • Purification and characterization of acid phosphatase V from Aspergillus nidulans.

    abstract::Acid phosphatase V of Aspergillus nidulans was purified by ammonium sulfate precipitation, gel filtration, and ion-exchange chromatography. The enzyme demonstrated a charge microheterogeneity on starch and acrylamide gel electrophoresis, but proved to be homogeneous on ultracentrifugation and gel filtration. Phosphata...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.110.1.246-255.1972

    authors: Harsanyi Z,Dorn GL

    更新日期:1972-04-01 00:00:00

  • Genetic mapping of the chromosomal replication origin of Salmonella typhimurium.

    abstract::Two hundred strains of Escherichia coli harboring Filv+ plasmids which carry a segment of the Salmonella typhimurium chromosome were isolated independently. Among them, two strains were found to harbor F' plasmids that are able to replicate in Hfr cells of E. coli; i.e., they carry a site designated poh (permissive on...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.138.2.297-304.1979

    authors: Joh K,Hiraga S

    更新日期:1979-05-01 00:00:00

  • Mapping of a structural gene for valyl-transfer ribonucleic acid synthetase in Escherichia coli by transduction.

    abstract::A structural gene, valS, for the valyl-transfer ribonucleic acid synthetase of Escherichia coli has been mapped on the clockwise side of pyrB and is closely linked to it. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.98.2.837-839.1969

    authors: Tingle MA,Neidhardt FC

    更新日期:1969-05-01 00:00:00

  • Glucose-triggered germination of Bacillus megaterium spores.

    abstract::Triggering of germination in Bacillus megaterium QM B1551 spores with D-glucose was studied. First, the interaction of glucose with spores for less than 1 min resulted in triggering almost 90% of the spores after the glucose was removed by dilution. Therefore only a brief time is needed for glucose to trigger germinat...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.138.2.442-445.1979

    authors: Racine FM,Dills SS,Vary JC

    更新日期:1979-05-01 00:00:00