Abstract:
:Copper is both a required micronutrient and a source of toxicity in most organisms, including Campylobacter jejuni Two proteins expressed in C. jejuni (termed CopA and CueO) have been shown to be a copper transporter and multicopper oxidase, respectively. We have isolated strains with mutations in these genes, and here we report that they were more susceptible to both the addition of copper in the growth media and to induced oxidative stress. Both mutant strains were defective in colonization of an avian host, and copper in the feed exacerbated the colonization deficiency. Overexpression of a cytoplasmic peptide derived from the normally periplasmic copper-binding region of CueO also caused copper intolerance compared to nonexpressing strains or strains expressing the non-copper-binding versions of the peptide. Taken together, the results indicate that copper toxicity in C. jejuni is due to a failure to effectively sequester cytoplasmic copper, resulting in an increase in copper-mediated oxidative damage.IMPORTANCE Copper is a required micronutrient for most aerobic organisms, but it is universally toxic at elevated levels. These organisms use homeostatic mechanisms that allow for cells to acquire enough of the element to sustain metabolic requirements while ensuring that lethal levels cannot build up in the cell. Campylobacter jejuni is an important foodborne pathogen that typically makes its way into the food chain through contaminated poultry. C. jejuni has a metabolic requirement for copper and encodes a copper detoxification system. In the course of studying this system, we have learned that it is important for avian colonization. We have also gained insight into how copper exerts its toxic effects in C. jejuni by promoting oxidative stress.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Gardner SP,Olson JWdoi
10.1128/JB.00208-18subject
Has Abstractpub_date
2018-10-10 00:00:00issue
21eissn
0021-9193issn
1098-5530pii
JB.00208-18journal_volume
200pub_type
杂志文章abstract::The chromatophores of Chromatium vinosum, as well as six other photosynthetic bacteria, contained two or more proteins which were insoluble when heated in the presence of sodium dodecyl sulfate (SDS) and 2-mercaptoethanol (beta-ME). When the chromatophores were dissolved at room temperature in SDS-beta-ME, these prote...
journal_title:Journal of bacteriology
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doi:10.1128/JB.138.1.207-217.1979
更新日期:1979-04-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JB.147.2.500-508.1981
更新日期:1981-08-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:
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pub_type: 杂志文章
doi:10.1128/JB.127.2.961-972.1976
更新日期:1976-08-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:1982-02-01 00:00:00
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pub_type: 杂志文章
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更新日期:1995-11-01 00:00:00
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pub_type: 杂志文章
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更新日期:1969-01-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JB.93.1.237-244.1967
更新日期:1967-01-01 00:00:00
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更新日期:1994-03-01 00:00:00
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pub_type: 杂志文章
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更新日期:1984-03-01 00:00:00
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pub_type: 杂志文章
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.167.3.805-808.1986
更新日期:1986-09-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1128/JB.135.2.490-497.1978
更新日期:1978-08-01 00:00:00
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pub_type: 杂志文章
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2013-10-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JB.133.3.1527-1529.1978
更新日期:1978-03-01 00:00:00
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