Mutational analysis of Burkholderia thailandensis quorum sensing and self-aggregation.

abstract：:Polynucleotide phosphorylase (PNP) plays a central role in RNA degradation, generating a pool of ribonucleoside diphosphates (rNDPs) that can be converted to deoxyribonucleoside diphosphates (dNDPs) by ribonucleotide reductase. We report here that spontaneous mutations resulting from replication errors, which are norm...

journal_title：Journal of bacteriology

authors： Becket E,Tse L,Yung M,Cosico A,Miller JH

更新日期：2012-10-01 00:00:00

abstract：:Glutamine phosphoribosylpyrophosphate amidotransferase is stable in growing cells, but is inactivated in an oxygen-dependent process at various rates in starving or antibiotic-treated cells. On the basis of studies of the purified enzyme, we suggested (D.A. Bernlohr and R.L. Switzer, Biochemistry 20:5675-5681, 1981) t...

journal_title：Journal of bacteriology

authors： Bernlohr DA,Switzer RL

更新日期：1983-02-01 00:00:00

abstract：:The genes for biosynthesis of the branched-chain amino acids leucine, isoleucine, and valine in Lactococcus lactis subsp. lactis NCDO2118 were characterized by cloning, complementation in Escherichia coli and Bacillus subtilis, and nucleotide sequence analysis. Nine structural genes are clustered on a 12-kb DNA fragme...

journal_title：Journal of bacteriology

authors： Godon JJ,Chopin MC,Ehrlich SD

更新日期：1992-10-01 00:00:00

abstract：:The genes encoding ammonia monooxygenase (amoCAB), hydroxylamine oxidoreductase (hao), and the c-type cytochrome c-554 (hcy) are present in multiple copies in the genome of Nitrosomonas europaea. The upstream regions of the two copies of amoC, the three copies of hao, and one copy of hcy were cloned and sequenced. Pri...

journal_title：Journal of bacteriology

authors： Hommes NG,Sayavedra-Soto LA,Arp DJ

更新日期：2001-02-01 00:00:00

abstract：:The replication order of markers was studied in Bacillus subtilis strains bearing the trpE26 mutation by the use of the density transfer technique. An important difference in this order was observed in comparison with that of strain 168 T-. All markers tested of a chromosome segment extending from trpD to ilvA replica...

journal_title：Journal of bacteriology

authors： Trowsdale J,Anagnostopoulos C

更新日期：1975-06-01 00:00:00

abstract：:The Rhizobium phaseoli recA gene has been cloned by interspecific complementation of the Fec phenotype of bacteriophage lambda. The cloned gene restored the recombination proficiency and conferred resistance to DNA-damaging agents (methyl methanesulfonate and nitrofurantoin) to an Escherichia coli recA mutant. The dir...

journal_title：Journal of bacteriology

authors： Martínez-Salazar JM,Romero D,Girard ML,Dávila G

更新日期：1991-05-01 00:00:00

abstract：:Periplasmic nitrate reductase (NapABC enzyme) has been characterized from a variety of proteobacteria, especially Paracoccus pantotrophus. Whole-genome sequencing of Escherichia coli revealed the structural genes napFDAGHBC, which encode NapABC enzyme and associated electron transfer components. E. coli also expresses...

journal_title：Journal of bacteriology

authors： Stewart V,Lu Y,Darwin AJ

更新日期：2002-03-01 00:00:00

abstract：:In Pseudomonas aeruginosa, flagellar genes are regulated in a cascade headed by FleQ, an NtrC/NifA-type activator. FleQ and RpoN positively regulate expression of flhA, fliE, fliL, and fleSR genes, among others. Direct interaction of FleQ with flhA, fliE, fliL, and fleSR promoters was demonstrated by gel shift assay, ...

journal_title：Journal of bacteriology

authors： Jyot J,Dasgupta N,Ramphal R

更新日期：2002-10-01 00:00:00

abstract：:The effect of a mutation in luxI (autoinducer synthetase gene) on transcription of luxR in the cloned Vibrio fischeri lux system (luxR, luxICDABE) was examined in Escherichia coli. For the luxI mutant, transcription from the luxR promoter (monitored with beta-galactosidase levels from a luxR::lacZ fusion, with LuxR su...

journal_title：Journal of bacteriology

authors： Dunlap PV,Ray JM

更新日期：1989-06-01 00:00:00

abstract：:Bacteria within the Mycobacterium avium complex are prominent in the environment and are a source of serious disseminated infections in patients with AIDS. Serovars of the M. avium complex are distinguished from all other mycobacteria and from one another by the presence of highly antigenic glycolipids, the glycopepti...

journal_title：Journal of bacteriology

authors： Belisle JT,Pascopella L,Inamine JM,Brennan PJ,Jacobs WR Jr

更新日期：1991-11-01 00:00:00

abstract：:A strain of Bacillus subtilis, UVSSP-42-1, which produces ultraviolet (UV)-sensitive spores and vegetative cells, was found to possess germinated spores 25 times more UV resistant than the resting spores. This relative resistance achieved upon germination was associated with the transition of the heat-resistant refrac...

journal_title：Journal of bacteriology

authors： Munakata N

更新日期：1974-10-01 00:00:00

abstract：:There are two closely related hik31 operons involved in signal transduction on the chromosome and the pSYSX plasmid in the cyanobacterium Synechocystis sp. strain PCC 6803. We studied the growth, cell morphology, and gene expression in operon and hik mutants for both copies, under different growth conditions, to exami...

journal_title：Journal of bacteriology

authors： Nagarajan S,Sherman DM,Shaw I,Sherman LA

更新日期：2012-01-01 00:00:00

abstract：:In Escherichia coli cultures maximally inhibited with chloramphenicol, formation of polypeptides still continued at a slow, constant rate for at least 90 min. The rate of leucine incorporation was reduced to 0.5%, but methionine was only reduced to 2%, suggesting that chains are normally initiated with methionine but ...

journal_title：Journal of bacteriology

authors： Cremer K,Silengo L,Schlessinger D

更新日期：1974-05-01 00:00:00

abstract：:The herpes simplex virus thymidine kinase gene has been cloned into a chimeric yeast plasmid cloning vehicle and transformed into appropriate yeast strains. Plasmids carrying the herpes simplex virus thymidine kinase gene can be propagated as autonomously replicating plasmids, but no RNA specific to the thymidine kina...

journal_title：Journal of bacteriology

authors： Kiss GB,Pearlman RE,Cornish KV,Friesen JD,Chan VL

更新日期：1982-02-01 00:00:00

abstract：:In Escherichia coli anaerobic growth lowers the basal or induced levels of numerous enzymes associated with aerobic metabolism. Mutations in arcA (dye) at min 0 relieve this pleiotropic anaerobic repression and render the cell sensitive to the redox dye toluidine blue. In this study we identified a second pleiotropic ...

journal_title：Journal of bacteriology

authors： Iuchi S,Cameron DC,Lin EC

更新日期：1989-02-01 00:00:00

abstract：:Balish, Edward (Argonne National Laboratory, Argonne, Ill.), and George Svihla. Ultraviolet microscopy of Candida albicans. J. Bacteriol. 92:1812-1820. 1966.-Yeast and mycelial strains of Candida albicans were grown in medium supplemented with sulfur amino acids in an effort to determine factors that control the morph...

journal_title：Journal of bacteriology

authors： Balish E,Svihla G

更新日期：1966-12-01 00:00:00

abstract：:Sixteen species of enterobacteria have been screened for mutagenic DNA repair activity. In Escherichia coli, mutagenic DNA repair is encoded by the umuDC operon. Synthesis of UmuD and UmuC proteins is induced as part of the SOS response to DNA damage, and after induction, the UmuD protein undergoes an autocatalytic cl...

journal_title：Journal of bacteriology

authors： Sedgwick SG,Ho C,Woodgate R

更新日期：1991-09-01 00:00:00

abstract：:We have sequenced the entire region of DNA required for the biosynthesis of CS5 pili from enterotoxigenic Escherichia coli O115:H40 downstream of the major subunit gene, designated csfA (for coli surface factor five A). Five more open reading frames (ORFs) (csfB, csfC, csfE, csfF, and csfD) which are transcribed in th...

journal_title：Journal of bacteriology

authors： Duthy TG,Staendner LH,Manning PA,Heuzenroeder MW

更新日期：1999-09-01 00:00:00

abstract：:The complete sequences of the RhsB and RhsC elements of Escherichia coli K-12 have been determined. These sequence data reveal a new repeated sequence, called H-rpt (Hinc repeat), which is distinct from the Rhs core repetition that is found in all five Rhs elements. H-rpt is found in RhsB, RhsC, and RhsE. Characteriza...

journal_title：Journal of bacteriology

authors： Zhao S,Sandt CH,Feulner G,Vlazny DA,Gray JA,Hill CW

更新日期：1993-05-01 00:00:00

abstract：:Caldwell, D. R. (U.S. Department of Agriculture, Beltsville, Md.), D. C. White, M. P. Bryant, and R. N. Doetsch. Specificity of the heme requirement for growth of Bacteroides ruminicola. J. Bacteriol. 90:1645-1654. 1965.-Previous studies suggested that most strains of Bacteroides ruminicola subsp. ruminicola require h...

journal_title：Journal of bacteriology

authors： Caldwell DR,White DC,Bryant MP,Doetsch RN

更新日期：1965-12-01 00:00:00

abstract：:The deacetylcephalosporin C synthetase (hydroxylase) gene from Streptomyces clavuligerus has been cloned and sequenced. The open reading frame codes for a protein with an Mr of 34,584. The hydroxylase gene (cefF) is closely linked to the epimerase gene (cefD) and the expandase gene (cefE) and is transcribed in the opp...

journal_title：Journal of bacteriology

authors： Kovacevic S,Miller JR

更新日期：1991-01-01 00:00:00

abstract：:The metD D-methionine transporter locus of Escherichia coli was identified as the abc-yaeE-yaeC cluster (now renamed metNIQ genes). The abc open reading frame is preceded by tandem MET boxes bracketed by the -10 and -35 boxes of a promoter. The expression driven by this promoter is controlled by the MetJ repressor and...

journal_title：Journal of bacteriology

authors： Gál J,Szvetnik A,Schnell R,Kálmán M

更新日期：2002-09-01 00:00:00

abstract：:It was previously reported that unlike the other obg/cgtA GTPases, the Vibrio harveyi cgtAV is not essential. Here we show that cgtAV was not disrupted in these studies and is, in fact, essential for viability. Depletion of CgtAV did not result in cell elongation. CgtAV is associated with the large ribosomal particle....

journal_title：Journal of bacteriology

authors： Sikora AE,Zielke R,Datta K,Maddock JR

更新日期：2006-02-01 00:00:00

abstract：:Plasmid ColIb-P9 of the I alpha incompatibility group is known to encode a DNA primase that acts in the conjugal transfer of the plasmid and can substitute for mutant dnaG gene product in vegetative replication of the Escherichia coli chromosome. The relevant genetic determinant (sog) has previously been cloned into a...

journal_title：Journal of bacteriology

authors： Dalrymple BP,Boulnois GJ,Wilkins BM,Orr E,Williams PH

更新日期：1982-07-01 00:00:00

abstract：:Partial denaturation of Saccharomyces cerevisiae chromosomal DNA was found to occur spontaneously during meiosis. Short regions of strand separation (300 base pairs long) were seen in DNA molecules prepared for electron microscopy by the aqueous spreading technique. These regions were clustered along the DNA. The time...

journal_title：Journal of bacteriology

authors： Klein HL,Byers B

更新日期：1978-05-01 00:00:00

abstract：:The Streptomyces coelicolor absA two-component system was initially identified through analysis of mutations in the sensor kinase absA1 that caused inhibition of all four antibiotics synthesized by this strain. Previous genetic analysis had suggested that the phosphorylated form of AbsA2 acted as a negative regulator ...

journal_title：Journal of bacteriology

authors： Ryding NJ,Anderson TB,Champness WC

更新日期：2002-02-01 00:00:00

abstract：:A cell permeabilization procedure is described that reduces viability less than 10% and does not significantly reduce the rates of ribonucleic acid and protein synthesis when appropriately supplemented. Permeabilization abolishes the normal stringent coupling of protein and ribonucleic acid synthesis. ...

journal_title：Journal of bacteriology

authors： Atherly AG

更新日期：1974-06-01 00:00:00

abstract：:An 8,883-bp mini-pXO1 plasmid containing a replicon from Bacillus anthracis pXO1 (181.6 kb) was identified by making large deletions in the original plasmid using a newly developed Cre-loxP system. Portions of the truncated mini-pXO1 were cloned into an Escherichia coli vector unable to replicate in B. anthracis. A 5....

journal_title：Journal of bacteriology

authors： Pomerantsev AP,Camp A,Leppla SH

更新日期：2009-08-01 00:00:00

abstract：:We have used a plasmid-based transcriptional reporter system to examine the transcriptional effects of 33 single point mutations in the box A region (TATA-like sequence) of the Haloferax volcanii tRNA(Lys) promoter. The most pronounced effects on transcriptional efficiency were found when the nucleotides corresponding...

journal_title：Journal of bacteriology

authors： Palmer JR,Daniels CJ

更新日期：1995-04-01 00:00:00

abstract：:The two 23S rRNA-5S rRNA-tRNAGly operons from the extreme thermophilic eubacterium Thermus thermophilus HB8 were used to characterized the in vivo processing and termination of 23S rRNA-5S rRNA-tRNAGly primary transcripts in this organism by nuclease S1 mapping. A processing site in the pre-23S rRNA 3'-flanking region...

journal_title：Journal of bacteriology

authors： Hartmann RK,Toschka HY,Erdmann VA

更新日期：1991-04-01 00:00:00