Isolation and expression of a gene cluster responsible for biosynthesis of the glycopeptidolipid antigens of Mycobacterium avium.

abstract：:Desulfurococcus kamchatkensis is an anaerobic organotrophic hyperthermophilic crenarchaeon isolated from a terrestrial hot spring. Its genome consists of a single circular chromosome of 1,365,223 bp with no extrachromosomal elements. A total of 1,474 protein-encoding genes were annotated, among which 205 are exclusive...

journal_title：Journal of bacteriology

authors： Ravin NV,Mardanov AV,Beletsky AV,Kublanov IV,Kolganova TV,Lebedinsky AV,Chernyh NA,Bonch-Osmolovskaya EA,Skryabin KG

更新日期：2009-04-01 00:00:00

abstract：:The crystal structure of Escherichia coli PdxY, the protein product of the pdxY gene, has been determined to a 2.2-A resolution. PdxY is a member of the ribokinase superfamily of enzymes and has sequence homology with pyridoxal kinases that phosphorylate pyridoxal at the C-5' hydroxyl. The protein is a homodimer with ...

journal_title：Journal of bacteriology

authors： Safo MK,Musayev FN,Hunt S,di Salvo ML,Scarsdale N,Schirch V

更新日期：2004-12-01 00:00:00

abstract：:We investigated the capacity of the hyperthermophile Pyrococcus furiosus for DNA repair by measuring survival at high levels of 60Co gamma-irradiation. The P. furiosus 2-Mb chromosome was fragmented into pieces ranging from 500 kb to shorter than 30 kb at a dose of 2,500 Gy and was fully restored upon incubation at 95...

journal_title：Journal of bacteriology

authors： DiRuggiero J,Santangelo N,Nackerdien Z,Ravel J,Robb FT

更新日期：1997-07-01 00:00:00

abstract：:The recent discovery that the protein DksA acts as a coregulator of genes controlled by ppGpp led us to investigate the similarities and differences between the relaxed phenotype of a ppGpp-deficient mutant and the phenotype of a strain lacking DksA. We demonstrate that the absence of DksA and ppGpp has similar effect...

journal_title：Journal of bacteriology

authors： Magnusson LU,Gummesson B,Joksimović P,Farewell A,Nyström T

更新日期：2007-07-01 00:00:00

abstract：:Parnas, Józef (Academy of Medicine, Lublin, Poland). Differentiation of brucellae by the aid of phages. J. Bacteriol. 82:319-325. 1961.-There appears to be no absolute specificity of phages for the three species of the genus Brucella. The phage test is only supplementary to biochemical and serological methods for spec...

journal_title：Journal of bacteriology

authors： PARNAS J

更新日期：1961-09-01 00:00:00

abstract：:Pseudomonas aeruginosa is an opportunistic bacterial pathogen which poses a major threat to long-term-hospitalized patients and individuals with cystic fibrosis. The capacity of P. aeruginosa to form biofilms is an important requirement for chronic colonization of human tissues and for persistence in implanted medical...

journal_title：Journal of bacteriology

authors： Vallet I,Diggle SP,Stacey RE,Cámara M,Ventre I,Lory S,Lazdunski A,Williams P,Filloux A

更新日期：2004-05-01 00:00:00

abstract：:In Escherichia coli, the following genes are involved in motility and chemotaxis. The H gene is the structural gene for flagellin. Mutation in the mot gene results in paralysis of the flagella, and mutation in the fla genes leads to an absence of flagella. The cheA, cheB, and cheC genes are required for chemotaxis. Th...

journal_title：Journal of bacteriology

authors： Armstrong JB,Adler J

更新日期：1969-01-01 00:00:00

abstract：:When cells of Escherichia coli THU were starved for thymine, they continued to grow without division for at least two successive volume doublings at their initial rate. Within experimental error this average rate of volume increase, 0.21 mum(3) per hr, was identical with that observed in control cultures during two ge...

journal_title：Journal of bacteriology

authors： Kubitschek HE

更新日期：1971-02-01 00:00:00

abstract：:The respiratory metabolism of Schizosaccharomyces pombe 972h(-), a fission, haplontic, "petite negative" yeast, was studied. Glucose and glycerol are good growth substrates and are oxidized under appropriate conditions. l-Lactate, ethanol, malate, and succinate are oxidized but are poor substrates for growth. d-Lactat...

journal_title：Journal of bacteriology

authors： Heslot H,Goffeau A,Louis C

更新日期：1970-10-01 00:00:00

abstract：:Lockart, Royce Z., Jr. (The University of Texas, Austin). Variations in the abilities of several lines of L cells to produce and to be affected by interferon. J. Bacteriol. 89:117-122. 1965.-The amounts of interferon produced by three different lines of L cells and mouse embryonic cells when inoculated with two strain...

journal_title：Journal of bacteriology

authors： LOCKART RZ Jr

更新日期：1965-01-01 00:00:00

abstract：:The folded chromosome of Escherichia coli has been investigated under various lysis and physiological conditions. A new gradient system was devised that allows excellent separation between unlysed cells and envelope-associated and envelope-free chromosomes. Isotope incorporation experiments showed that the fraction of...

journal_title：Journal of bacteriology

authors： Korch C,Ovrebo S,Kleppe K

更新日期：1976-08-01 00:00:00

abstract：:The metE gene of Salmonella typhimurium was cloned into plasmid pACYC184 by using a lambda gt7 -metE transducing phage as a source of metE DNA. The recombinant plasmid, designated pGS41 , carries a 12.8-kilobase-pair EcoRI insert fragment. The metE gene was subcloned from pGS41 into plasmid pBR322 on a 4.2-kilobase-pa...

journal_title：Journal of bacteriology

authors： Schulte LL,Stauffer LT,Stauffer GV

更新日期：1984-06-01 00:00:00

abstract：:We report the draft genome sequence of Paenisporosarcina sp. strain TG-20, which is 4.12 Mb in size and consists of 4,071 protein-coding genes and 76 RNA genes. The genome sequence of Paenisporosarcina sp. TG-20 may provide useful information about molecular adaptations that enhance survival in icy subsurface environm...

journal_title：Journal of bacteriology

authors： Lee JH,Koh HY,Lee SG,Doyle S,Christner BC,Kim HJ

更新日期：2012-12-01 00:00:00

abstract：:Chlamydia spp. express a functional type III secretion system (T3SS) necessary for pathogenesis and intracellular growth. However, certain essential components of the secretion apparatus have diverged to such a degree as to preclude their identification by standard homology searches of primary protein sequences. One e...

journal_title：Journal of bacteriology

authors： Betts HJ,Twiggs LE,Sal MS,Wyrick PB,Fields KA

更新日期：2008-03-01 00:00:00

abstract：:When Legionella pneumophila grows on agar plates, it secretes a surfactant that promotes flagellum- and pilus-independent "sliding" motility. We isolated three mutants that were defective for surfactant. The first two had mutations in genes predicted to encode cytoplasmic enzymes involved in lipid metabolism. These ge...

journal_title：Journal of bacteriology

authors： Stewart CR,Burnside DM,Cianciotto NP

更新日期：2011-11-01 00:00:00

abstract：:The bacteriophage T4 MotA protein is a transcriptional activator of T4-modified host RNA polymerase and is required for activation of the middle class of T4 promoters. MotA alone binds to the -30 region of T4 middle promoters, a region that contains the MotA box consensus sequence [(t/a)(t/a)TGCTT(t/c)A]. We report th...

journal_title：Journal of bacteriology

authors： Gerber JS,Hinton DM

更新日期：1996-11-01 00:00:00

abstract：:Spendlove, Rex S. (California State Department of Public Health, Berkeley), Edwin H. Lennette, Charles O. Knight, and Jean N. Chin. Production in FL cells of infectious and potentially infectious reovirus. J. Bacteriol. 92:1036-1040. 1966.-A comparative study was made of the development in, and release from, FL cells ...

journal_title：Journal of bacteriology

authors： Spendlove RS,Lennette EH,Knight CO,Chin JN

更新日期：1966-10-01 00:00:00

abstract：:A mutant (pgi) of Coprinus macrorhizus deficient in phosphoglucose isomerase did not grow on fructose and grew poorly on glucose. The pgi mutation inhibited the formation of monokaryotic and dikaryotic fruiting bodies. ...

journal_title：Journal of bacteriology

authors： Nyunoya H,Ishikawa T

更新日期：1980-10-01 00:00:00

abstract：:There are two closely related hik31 operons involved in signal transduction on the chromosome and the pSYSX plasmid in the cyanobacterium Synechocystis sp. strain PCC 6803. We studied the growth, cell morphology, and gene expression in operon and hik mutants for both copies, under different growth conditions, to exami...

journal_title：Journal of bacteriology

authors： Nagarajan S,Sherman DM,Shaw I,Sherman LA

更新日期：2012-01-01 00:00:00

abstract：:A series of mutations of the P1 plasmid prophage that lead to increased copy number was isolated and analyzed. The copy number of the mutants was elevated at least five- to eightfold relative to wild-type P1, as determined by single-cell resistance to antibiotics, activity of enzymes, content of superhelical DNA, and ...

journal_title：Journal of bacteriology

authors： Scott JR,Kropf MM,Padolsky L,Goodspeed JK,Davis R,Vapnek D

更新日期：1982-06-01 00:00:00

abstract：:A 3.3-kilobase PvuII fragment carrying the PHR1 gene of Saccharomyces cerevisiae has been cloned into an Escherichia coli expression vector and introduced into E. coli strains deficient in DNA photolyase. Complementation of the E. coli phr-1 mutation was observed, strongly suggesting that the yeast PHR1 gene encodes a...

journal_title：Journal of bacteriology

authors： Sancar GB

更新日期：1985-02-01 00:00:00

abstract：:The force required to rupture bonds between individual Staphylococcus aureus MSCRAMMs and surfaces coated with extracellular matrix molecules has been quantified by using optical tweezers. The observed binding forces between fibrinogen or fibronectin and S. aureus MSCRAMMs occurred as an approximate integer multiple o...

journal_title：Journal of bacteriology

authors： Simpson KH,Bowden G,Höök M,Anvari B

更新日期：2003-03-01 00:00:00

abstract：:Cell walls of 19 Micromonospora species were analyzed for their components. All the cell walls had xylose and arabinose, but the presence of glucose, galactose, mannose, or rhamnose depended on the strain. Amino acids present in the walls consisted of glycine, glutamic acid, diaminopimelic acid, and alanine, in a mola...

journal_title：Journal of bacteriology

authors： Kawamoto I,Oka T,Nara T

更新日期：1981-05-01 00:00:00

abstract：:Rhizobium sp. strain NGR234 produces a large family of lipochitooligosaccharide Nod factors carrying specific substituents. Among them are 3-O- (or 4-O-) and 6-O-carbamoyl groups, an N-methyl group, and a 2-O-methylfucose residue which may bear either 3-O-sulfate or 4-O-acetyl substitutions. Investigations on the gene...

journal_title：Journal of bacteriology

authors： Quesada-Vincens D,Fellay R,Nasim T,Viprey V,Burger U,Prome JC,Broughton WJ,Jabbouri S

更新日期：1997-08-01 00:00:00

abstract：:We have compared the amino acid incorporating activities of extracts of Escherichia coli and Salmonella typhimurium in in vitro protein-synthesizing systems directed by bacterial messenger ribonucleic acid (mRNA) of both species and by the genomes of coliphages Qbeta and f2. E. coli and S. typhimurium extracts transla...

journal_title：Journal of bacteriology

authors： Bassel BA,Curry ME

更新日期：1973-11-01 00:00:00

abstract：:Cells of the Escherichia coli dnaE(Ts) dnaE74 and dnaE486 mutants die after 4 h of incubation at 40 degrees C in Luria-Bertani medium. Cell death is preceded by elongation, is inhibited by chloramphenicol, tetracycline, or rifampin, and is dependent on cell density. Cells survive at 40 degrees C when they are incubate...

journal_title：Journal of bacteriology

authors： Strauss B,Kelly K,Dincman T,Ekiert D,Biesieda T,Song R

更新日期：2004-04-01 00:00:00

abstract：:A modified gel electrophoresis technique provided a reproducible way of detecting and isolating plasmids with molecular weights ranging from 12 X 10(6) to 370 X 10(6) for Azospirillum species. Analysis with the nifHD region of Rhizobium trifolii showed that the Azospirillum nif genes were chromosomally located in all ...

journal_title：Journal of bacteriology

authors： Plazinski J,Dart PJ,Rolfe BG

更新日期：1983-09-01 00:00:00

abstract：:The expression pattern of the Escherichia coli genome is controlled in part by regulating the utilization of a limited number of RNA polymerases among a total of its approximately 4,600 genes. The distribution pattern of RNA polymerase changes from modulation of two types of protein-protein interactions: the interacti...

journal_title：Journal of bacteriology

authors： Ishihama A,Kori A,Koshio E,Yamada K,Maeda H,Shimada T,Makinoshima H,Iwata A,Fujita N

更新日期：2014-08-01 00:00:00

abstract：:Bishop, D. G. (University of Sydney, Sydney, Australia), and J. L. Still. Fatty acid metabolism in Serratia marcescens. J. Bacteriol. 82:370-375. 1961.-A study has been made of the oxidation of saturated fatty acids containing between two and 18 carbon atoms by whole cells of the bacterium, Serratia marcescens. This o...

journal_title：Journal of bacteriology

authors： Bishop DG,Still JL

更新日期：1961-09-01 00:00:00

abstract：:Escherichia coli possesses three distinct formate dehydrogenase enzymes encoded by the fdnGHI, fdhF, and fdoGHI operons. To examine how two of the formate dehyrogenase operons (fdnGHI and fdhF) are expressed anaerobically in the presence of low, intermediate, and high levels of nitrate, nitrite, and formate, chemostat...

journal_title：Journal of bacteriology

authors： Wang H,Gunsalus RP

更新日期：2003-09-01 00:00:00